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Step 1: Select Comparison OptionsUse these options to refine parameters used for the comparisons (applies as long as at least 1 non-zero value is entered).
There are two types of comparison methods.
The first, sum of squares, does the following: For each previously performed blot, it calculates the sum of squares of the differences between user-entered intensity and previously determined intensity for all lanes. It then takes the square root of that value. Of the two comparison methods, this tends to yield more accurate results.
The second method, dot product, simpy calculates the dot product between the user-inputted intensities and previously determined intensities.
Step 2: Enter Blot Intensity ValuesDirectly below are three forms that correspond to three different multiple tissue northern blot types.
Each box in the form corresponds to a lane in the blot. Intensity values (integers or real numbers are accepted) must be entered for all lanes. These values will be normalized before being compared with database results.
Please complete the form that corresponds to the correct blot type and submit the query by pressing the button directly below the form that you have completed.
Alternatively, if you know the Gene Lynx ID of the gene that you are interested in, you may retrieve the mtn blot data for that gene directly by entering the ID into the form at the very bottom of the page.
Once the values are submitted, you will be presented with a table of resulting matches with scores.
Please note that scores are weighted differently for each comparison method. Eg. A score of 12.5 using the dot product method is not equivalent to a 12.5 using the sum of squares method. The maximum score for the dot product method is dependent on the inputted values. For completely uniform intensities (0.125 in each lane), for example, a perfect score would be 12.5. For the sum of squares method, the maximum score will always be 100.
Lastly, when an intensity of 0 is entered in all lanes, only exact matches will be reported.
Human 12-Lane (7780-1)
Human II (7759-1)
Find all bands for a particular gene (Enter a Gene Lynx ID)
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