LOCUS Ple151\(pEMS1172 19490 bp DNA circular 28-JUL-2008 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2683 - TCAGATGGCAACTTCAAAGTGGA Tm=56.2 Deg C Antisense primer: oEMS2674 - TGGTCAGGCTGCAACTCACG Tm=56.9 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 577bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|489076323| COMMENT VNTDBDATE|489076323| COMMENT LSOWNER| COMMENT VNTNAME|Ple151 (pEMS1172)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|George Yang| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#205=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -255 100) COMMENT (CObjectList) (CObjectList)) COMMENT #206=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -325 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #207=(AnlzFontBackColorItem 2709 2806 40304639) COMMENT #208=(AnlzFontBackColorItem 138 1154 40304639) COMMENT #209=(AnlzFontBackColorItem 1155 1520 50318387) COMMENT #210=(AnlzFontBackColorItem 1521 2244 33567743) COMMENT #211=(AnlzFontBackColorItem 2268 2708 33567743) COMMENT #212=(AnlzFontBackColorItem 2245 2267 43633766) COMMENT #213=(AnlzFontBackColorItem 2827 3180 40304639) COMMENT #214=(AnlzFontBackColorItem 2807 2826 43633766))) (CObList) 543453793 COMMENT (CStringList) 1380863812 825242196 (CObList))) FEATURES Location/Qualifiers misc_recomb 13353..17164 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker 18484..19484 /vntifkey="22" /label=AmpR misc_marker 11902..13327 /vntifkey="22" /label=AmpR misc_recomb 6085..11906 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4307..6084 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 13334..13351 /vntifkey="21" /label=I-Sce\I misc_feature 3189..3222 /vntifkey="21" /label=FRT\(F5mutant) misc_feature 3223..3233 /vntifkey="21" /label=Kozak\consensus misc_feature 3230..3949 /vntifkey="21" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 3950..3985 /vntifkey="21" /label=Tetracysteine\residue misc_feature 3986..4058 /vntifkey="21" /label=NLS misc_feature 4064..4097 /vntifkey="21" /label=FRT misc_feature 4251..4301 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..3180 /vntifkey="21" /label=Ple151 /note="originally named "OLIG1-D"" primer 2246..2268 /vntifkey="27" /label=oEMS2683 /note="sense primer in region 11" primer 2808..2827 /vntifkey="27" /label=oEMS2674 /note="antisense primer in region Prom" misc_feature 2710..3181 /vntifkey="21" /label=Prom\region misc_feature 139..1155 /vntifkey="21" /label=region\8 misc_feature 1156..1521 /vntifkey="21" /label=region\10 misc_feature 1522..2709 /vntifkey="21" /label=region\11 BASE COUNT 5220 a 4434 c 4375 g 5461 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggcctg ttcaactcgg atagtcctca ccactgagtt aatatttcca 181 ggtcagagcg cctccgagag aaaaaggcgg tggccttgga gctctggtct ccttaactgc 241 accctgggaa gtcttcacac ggcctttctt cccctggtgg tctaatctgg agtcagatcc 301 agccgtcagc tcttacagac ggggaaggac cgggagccaa cgggaaaaca acaggtgagc 361 ttgtgaacaa gtccccttct gtgtctcggg cagggtgtct gcccgggcaa ccgtggggct 421 gcactgactt ccagaacctt ctgcctctgc aggtgtttta ggaacttggc ctcagcgctt 481 cagtgaccca tattttatta cctttcaggt ttctataatt catcattcac tcctttctga 541 aaacctagaa tgaaaaagtt tagcagctca gcgccgcggc ctctctccat tctctcccca 601 aacacatcgg cctgtttgtt ctctttcatc cccgctcttc ctgttgcatt ctgccggttc 661 aatggggaag gagtgaaacc ggatggctgg acccagtgcg gggaaatgac ttcagagcca 721 cctttgtttc cctcattcct ttgagcgcac ttggccctgc gtctctatga aagggcttgt 781 ttgaaccatt cggaacatcc agcaagccag agatgctgcg gccagaaccg ctcgcatctt 841 ttcaggctgc ctgagactct gggttaaact gccaaatttt tgcaacaaaa ctaaccatga 901 cattggacat tgtgttataa attagccaca gccttccaag caaattgtct ctttttattg 961 tatcagtgta agcctcggaa acatttggct tcagctacta ccttcaggga ggctcagagg 1021 atatttttgt tctgccccaa accaactctt cagacaccga gttgcatgtg ctaataccca 1081 tatcttcatt cacagttatt cttatcacac tccctctgag aacctctgtc agttatgttc 1141 tatttctctg cctcccagaa ccttccttgt cctcacgctt gactagcaga caatgggttc 1201 ttcagaagcc acagcatttc agctgttttg gccccagagg ccacaagctg actgcatgtc 1261 attctccacc agcagagcgt cacctcgggg tagctccaaa cagtatcaac cggtttgtgg 1321 tgagtggata aacaccaggc tgggtgaatg aagtcacagg ctgagtcatc ctctgcacat 1381 gggggctgaa tggggctcag tcaggcccag aggagcctcc ccggcaaggt gctgggggcc 1441 aggctctccc tgccagtgag gctggggtcc gtccccaata gtcattcctt tggccaacaa 1501 acacttattg agcgcccacc tagctcttca agtcgcagat aacagtgaag gccctaagac 1561 tcaccatcca aaacagtttt ggaaaaaaac caaagaagag aaaataattt caaaccttaa 1621 agcaatgcat ggtgattctc tggggggtct aagaatttct cccagagaaa tgataaggca 1681 gacaaatgga agatcaatga agcaacatcg tggcaataat gcaattggtc ctgccgtagt 1741 catgaacatg taaataacaa gacagcaata agaagacccc agactctgcc tagaaacaaa 1801 atattcagcc tgttgttgtt gtttcttatt gcaggatttt gagtggaaaa gtagttccac 1861 ttgtcaatca gctacgttgc aggggctaat agattctggt gtttagggag gtcaacacgg 1921 aatttaattt aacatctcac aatacaccca catgcccaga aaatgtggac agcttgatag 1981 ggttcactga gccaaagaag caagacccaa acagtgcatt aatgtatagg ggaagaatag 2041 ttgcaatcag ccccttgtga attggacaga gggcttggag atgaagcaat tatcagccat 2101 cggggattaa tagtaaagtg tgaataccaa gctgtatatg ttaggggcag aatatgttgc 2161 ctatagaaaa caacaaagga cgtttcttac tattaagtga gattaaacgt aatggcctga 2221 tttaatcaca aaggcagaat taaattcaga tggcaacttc aaagtggaca caggaacaca 2281 ggccactgct tacatttaca tatagctggc atagcttggc ctgcaacaca ataggataaa 2341 gtttctttgg ccacttaaac aattttctat tcttcacctt tttctttttt tctttttttt 2401 ttcttttgtg ggtgtgtgtg tgtgtgtgtg tgtgtgtgaa cactcactgt atttattccc 2461 caattgcatt tagttagtaa gaaggagcta agtgttatat ttattcatgg aggctataac 2521 tgttaattct tccaaattgt gaaaaaaaag cctcataggg tcaataagaa cagagtagtt 2581 ataatcagat tgtcaccaaa aaagaaaaag aagaaaaaca attaaacaaa acaaacaaac 2641 aaaactttct agagtgaaca gtgtcttcta actttctctc ccttcaactt aagaattcag 2701 ctcttgagga cataagacgg taaattttat gttatgaata tgtgatcaca gttaaaaaaa 2761 aatatgaggg aggttgtttt tgagtagaca gttgctggag gaaggcacgt gagttgcagc 2821 ctgaccagtg cgaccagtcc tccttcaaca gccccgcttg ccttgaaaag gcgcccttac 2881 tccgctcagg gggcctgtcc cgcgcggctg gcacaggcgc tcacaataac ctcctaaagc 2941 ggtgccagcc gcacctccgc gcggccccgg cacaagcagc caatgaacac gcggctgcgc 3001 ccggcctcgc gcctccattg gctgcgcccc gccacccgct gccccgcagg ttcccaagcc 3061 gggtttaaag gggtagggcg cgggccaggg ccccaccatc gtttccccgc gcgcaggtcc 3121 gcggggaggg gcggcctgcc gaccggccca ccccagggcg ttcctgaagg gcgtcctcgg 3181 ggcgcgccga agttcctatt cttcaaaagg tataggaact tccgccacca tggtgagcaa 3241 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 3301 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 3361 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 3421 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 3481 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 3541 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 3601 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 3661 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 3721 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 3781 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 3841 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 3901 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagttat ttctgaactg 3961 ctgcccgggc tgctgcatgg aaccggatcc aaaaaagaag agaaaggtag atccaaaaaa 4021 gaagagaaag gtagatccaa aaaagaagag aaaggtaata taagaagttc ctattctcta 4081 gaaagtatag gaacttcagc ggccgcgact ctagatcata atcagccata ccacatttgt 4141 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 4201 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 4261 tagcatcaca aatttcacaa ataaagcatt tttttcactg cgaattctcg taaaactctt 4321 catggcagta gttattattc tctctctctc tctttttctt ttttcttgag acaggatatt 4381 tctctgttgc ccaggctgga gtgcagtggc acagtcttgg ctcactgcag cctggacctc 4441 ctgggctcaa gccatcctcc cacctcagcc tcccaagtag ctggggctac aggcacatgg 4501 ccaccaggcc cagataattt ttcatttttg tagagactga gtctcaccat gttacccagg 4561 tttattattc tcatttttta gatgaagaga ctgaggtcca gagaagctca atgacttgcc 4621 tagttttaca aatctcctgc catcacatac ccctcagcgt ccttaataag agggaggcca 4681 ccaactatgt gctgggcact gtggtggatg ctggagctat agggttgagt atataagaaa 4741 tggtgttgct ggagcaactg ttgcttgctt acctgaccta tctgagaatt aattagcagg 4801 ggaacatatt tttgttttca gattcaatat aagaacttgt gtgggcaaaa ataaagatca 4861 gtagtaataa cagtagttcc catttgctga ctgtactgtc ctaagtgcat atatatatac 4921 atacacacac gcatacctat actcctctaa tactcaaaat gatcctgttt atgtattgtt 4981 aatatgctca ttttattttt aaatttttat ttatttttat ttttatttat ttttgagacg 5041 gagtctcatt ctgtcgcgga ggctgaagtg cagtggtgcg atctcagctc agtgcgacct 5101 ccgcctcccg ggttcaagtg attctcctgc ctcagcctcc ggactagctg ggattacagg 5161 cgcccgcctc cacgcccagc taatttttgt atttttagta gagatggggt ttcgccatgt 5221 tggccaggct ggtctcgtac tcctgacctt gagtgatcca cctgcctcgg cctcccaaag 5281 tgctgggatt acaggcatga gccaccgcgc cgggctaata tgctcatttt agtgaggcaa 5341 aaatagaggc tcagagtctg atttgtacaa aactacagag cagttaagtg tcctctcaga 5401 tgtgtaccct gatctgggtg actctaggac tctaggtctc aactgttaca accagttaag 5461 ggtttgggga agcactgggc caagagtcag gaaaatggaa gccacaggta gtgcaaggtc 5521 ttgggaatgg gacgtctggt ccaaggattc acgcgatgac tggaacccga agagccgggg 5581 cccggtttac ggccgccatg aagcaacgcg cgccggtagg tttgggaatc agggagccct 5641 ctgaatagga gactgagttg ggagggaaag gggcttcgct gggggagcct cggcttcttc 5701 tgggagaaaa ttcccacggc tacctagtga gcctgcaaac tggtaggcgc cggcgtaggc 5761 gcgcgggcgg ggccgggggc ggggcctgcg gggcgtggcg gggcgggcag agggcggggc 5821 ctgcttctcc tcagcttcag gcggctgcga cgagccctca ggcgaacctc tcggctttcc 5881 cgcgcggcgc cgcctcttgc tgcgcctccg cctcctcctc tgctccgcca ccggcttcct 5941 cctcctgagc agtcagcccg cgcgccggcc ggctccgtta tggcgacccg cagccctggc 6001 gtcgtggtga gcagctcggc ctgccggccc tggccggttc aggcccacgc ggcaggtggc 6061 ggccgggccc tgaggcgcgg gatcgatcca tgacaccccc ccgcccccca tatctcacta 6121 taattcactg tcaaatggct tttatgattt ggagtttggg gatactttta aagacagggt 6181 cttggtagct cctagatggc ttgggattca cttagtaacc cttgctggcc taaaattcag 6241 gaacttctac ctcatcttcc caagtactgg gttagtaggg atgtccagca tatttagctt 6301 gctttaatga ttttgctgta cttttttctg tgtgtacagg tgcatgtata tttataaaac 6361 taacataata tattttaccc ccaaagtacc ataaccctaa ttaatgctat taaatgtttt 6421 gtatgctggg ctctgggcat actaatataa ccaatattat acaatgcaaa acaattttgg 6481 aatatcttct gtaaaattat cttattagta agttgtaacc atctcagact tctaagtttg 6541 agtttgtaaa ggatcagttt tttttggtga cctacacaca cacacacaca cacacacaca 6601 cacacacaca caggggggtg gattctgaga atttagagaa tttagatggt taataccagt 6661 ttttattttt attctgtgtg atatttattt attaagattt atttatttat tacatgtagg 6721 tacactgtat ctgtcttcag acacaccaga agagggcatc agatctcgtt acagatggtt 6781 gtgagtcacc atgtggttgc tgggatttga actcaggacc tctggaagaa cagtcagtgc 6841 tcttaaccgt tgagccatct caccagcccc gtgatatttt atttaactta ctagaatata 6901 ctgtgttaaa acatatttta ggggattggg gagatggccc attctgtaaa gtgtctgcca 6961 tgtcatcttc aggacctgcg tttgtaaccc cagcatggag aagacagaga cagggagacc 7021 cctgggcttc ctggcaagct agactagcaa atttggttaa ctaggttcaa tgagagactc 7081 agtctcaaaa cataagaagt gaatttttta gacccaattt caatctctga actccatacg 7141 tatgcacatg tacctgcata taaatatata tacacaccca taaacacatg cacacacaaa 7201 aaatggttca aaagttctta aaatgtataa gagtagaaag tgtagagccg ggagtggtgg 7261 cgcacgcctt taatcccagc acttgggagc agaggcaggt ggatttctga gttcgaggcc 7321 agcctggtct acagagtgag ttccaggaca gccagagcta tacagagaaa tcctgcctca 7381 aaaaaccaaa aataaagtgt atatactaat ctttcatgcc taaaatattt atttttgcca 7441 catttttcat acctctttaa tatttttctt tttctctttg tgcttcaaag gaaattttgt 7501 gtcatgtttt acttttcatt cttgactcta ttcagcagta agacgcagca tggctttctt 7561 agagaagtat aatctattac aaaacggtaa catttaactg ctctacatac cctcttgtag 7621 ctaaattcct ctgttaaact aagacttatt atctaaacgt aattaaatgt tgatatctta 7681 taatgctcca ctttgaaaca gctgtctttc ttttgcagat tagcgatgat gaaccaggtt 7741 atgacctaga tttgttttgt atacctaatc attatgccga ggatttggaa aaagtgttta 7801 ttcctcatgg actgattatg gacaggttag taagacctcg attgaagtta tttttagagg 7861 gtgtgtgtgt ggtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg taagagagag 7921 aggagaggca tgtgtgatat atatgtgaag tgtattgcat gtacatggaa tccagagaag 7981 gacattaggt gtcctactct gttgctctct tattcccttg aatcagtgtc tcttgctgaa 8041 cctggggcat acatactgat ttggctaggc tgcctggcta gcaagaccca gggatctgct 8101 tgtctctgcc ccccagtgct gacgttacaa gcaggtgcaa ccacatctgg tttttatatg 8161 ggtactgggg gatctgaact tagggcctga tgcgtgccca agtgctctca cccgccaagc 8221 cacctctcca gccaaagggt tttttttctt gtattaattc taacttagca actgttgagt 8281 agttgatgtt tgaatataag ctaagccaac ttattagaat aaatgttttc tattttttac 8341 ttgtatgtgt gagtgcctgc atgtatgtat gtgaagcata tgtattccag gatgtcagaa 8401 ttgtgatgga ttccctggaa ctggagttac agaccattgt gaaccactgt gtatgggtgc 8461 tgggaactga actttaggtc ctctgcaaaa acagtaagga tcttaactga taaactatct 8521 ttctagccct aaggctttct acttttaaag ttcgtttctc tttctctctc tctctctctc 8581 tctctctctc tctctccctc tccctccctc ctcctcctcc tcctctttcc cccctctcgc 8641 ctcctctttc tctctcccct ccctctctct ctctcacaca cacacacaga cagagagagg 8701 agagagaaag gaagccttac taattaatta cctagtttta gacataacta gtgggaatcc 8761 atagtaagta aatcaacatt cttgaagaga ggttagaaga attggctctc gttgtccatc 8821 gaggctgtat aagattattc ttctgttttc tcagcttacc atacatattt tctacgtaga 8881 caataccaat tcaaatacac tgtacgtaaa gtatatgcgc acacacacac acacacacac 8941 acacacacac acacaagtta catattgtca tatagtacta aaatttatgg actttttcat 9001 attaatacat ctagagctat catgtttttt atcttttaaa aattacttta cggggctaca 9061 gagatgtctc agcagttagg agcactgact gctcttccag aggtcctgag ttcaattccc 9121 agcaaccaca tggtggctca caaccatctg taatgggatc tgatccactc ttatggtgtg 9181 tctgaagaca gtgacagtgt actcatatac atgaaataaa tctttaaaaa attactttac 9241 catcagggta tatatatata tatatatgtg tgtgtgtgtg tgtgtgtgta tgaagtattt 9301 gctatttgag agggtctcac tgtttagctc agtctggctt ggaacgttct gtatagccca 9361 gactggcctc aactcagcag tccccctgaa aggctctcat atgtggagat tacaggtatg 9421 tgctactgtg cccagcccta catgttactt tgtttttgaa atagggtatc aaatagctta 9481 gactggcttc taagtcagtg tgtagctaag gatggtctga gcttctgaac ctgctacttc 9541 aactcctggt gtgccatcat ggccagtttg cacagttgtg agagtagaac tcaggacctc 9601 ctatgtgtta ctagacatgt tctctaccag ctgagctaca tcccaattcg cctacattta 9661 cttttatttt ttaaaagatt tatttatgta tacagcactc tgcctgcatg tacatctgca 9721 tgccagaaga gggcaccaga tctcatcata catagttgtg agccaccatg tggttgctgg 9781 ggattgaact cagggcctct ggaagagcag tcagtgctct taacctctga gccatctctg 9841 tagcccccta catttatttt aaagattata ggctattcta ttcatagatg tgccataact 9901 gatttaacca gatcttcatt attttgtact tagtagagta tattagaggg aatgaatctt 9961 aaatacacat tttgacactt gctcagcttt agacaagtaa aaatcactgg tcaaggaaat 10021 ggtgcttagt ggatgcttct gaactgtcaa attgtcctcc aacaagttac atcattttat 10081 acttccaacg ttatgtgaga gttcattttt ctttaggctc ttctagtatt ttatatttga 10141 gaaataaaat ttaaccaaaa tttgccaatc tagtaagctt gtttttattt gtatctttaa 10201 attttatgat agatgttata gtgtactctc ctctcccctc ccctcccctc tcctcctcag 10261 caggatcttg ttatgtagcc ctaagggatc taatacttac tctgtagtcc aggctgtcct 10321 tcagctcatg agacacacca tcatgcttgg catgtttctt gttctgaaag gtatctggtt 10381 ttaattttgg ttgagctggt gtgttggtgt atatctgtaa tctcagcaca ggcaagtaga 10441 gacaaggatt atgattacag tccagcctgg gctatttaga gggattctct ttaatctttc 10501 ctcatgcccc aaaatcttac ctttggtata tgaaaaatag tctccacttc tgcaaaatat 10561 tgctttatga agtaagaatt aattcccttc atagagacaa ggaatgtgtc ctgtaaaagt 10621 ttaatgtgta agaagtattt gttataaaag ataaatattc agaatcttct ttttaattcc 10681 tgattttatt tctataggac tgaaagactt gctcgagatg tcatgaagga gatgggaggc 10741 catcacattg tggccctctg tgtgctcaag gggggctata agttctttgc tgacctgctg 10801 gattacatta aagcactgaa tagaaatagt gatagatcca ttcctatgac tgtagatttt 10861 atcagactga agagctactg tgtaagtata attaacttat aattaaaaaa atagggccat 10921 tctagtttta tctatatttt ttttaaactt gtgcaaacta tgctacataa tcaacttact 10981 tgattatgta atatatatag taatagttgg aattactatt attacatatt tatttttctg 11041 tgctggggta gaacccaggg aactaagtac tgtaataacc gagcttcatt catcctagtc 11101 atttttaatt tttttttttt tttttgtttt ttcgagacaa ggtttctctg tgtagccctg 11161 gctgtcctgg aactcactct gtagaccagg ctggcctcga actcagaaat ctgcctgcct 11221 ctgcctccca gtgctgagat taatgacgtg caccaccact gcctggcatt tttttaaagt 11281 tttaatttga gaccctttct cactaacttg ctcacactat cctcgaactc attacgtggg 11341 ctggtctaga aatttcagtc aacttgcctc agcttctcaa gtagctggga ttacaggcag 11401 gtgctaccac ttctggccat gatcattgtt gttgttgcta ttattattat tattatttca 11461 ctgatacttc atatcacaac acagaatagt aactgtagtt catgaccaac aaaaaagttg 11521 tacagtttct ctgttgtctt attttgatct ggattttgat ttgtttcatg gcactgggca 11581 ggctaatctc aaactgcatg gctcaaatag tccttctgtc ttaatctccc atgttctgga 11641 agtataggta tcactacacc tagctgtatt ttcatatttt aaaaaaaacg cctaagaatt 11701 gctattgaat gaggcggaga gatggctcag cagtcacaag tacttgtaga ggactggggt 11761 ttggttacta gcacctcaaa gatggcttac aattatctgt agctccaatt ccagaggatc 11821 tgtgtggtaa agctgcatgt atatgggcat gcaggtaaaa ctgctaaaca tataaaaata 11881 gctcagtctt aagagaattg gtcgactttt tcgccctttg acgttggagt ccacgttctt 11941 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 12001 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 12061 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 12121 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 12181 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 12241 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 12301 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 12361 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 12421 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 12481 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 12541 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 12601 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 12661 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 12721 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 12781 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 12841 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 12901 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 12961 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 13021 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 13081 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 13141 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 13201 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 13261 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 13321 ctaccaggtc gagtagggat aacagggtaa tggatcctcg ttgggcacct gcactcatgt 13381 acgcaggccc acatatagat gtacacacgc atatcattca aaatagaaat tatttataca 13441 agtagtgcgt tggagagctg gctcaggagt tgttaatgct tgctaccctt gcaaaggacc 13501 caagttcaat ttccagcacc tgtgttgggc atctcacaac cacatgtaaa tctagtttca 13561 ggggatttca cccctcttct gaactcttca ggcacttata cccacaaatg gacatctcca 13621 catagagaca catactttta aaaaagtaat ctagggtcta gagaaatggt tatagaccac 13681 ggactgggat cagttcccag aagccacatc ctgtggctca caattgcctg tgaatcaagt 13741 tctagatctg gtggcctcgt ctagcctgca gtcatcaaca aacacatgca cacatccata 13801 cacacataat ttaaaagaaa gcgaaagtaa cctataatgc taaacaaagc acattctcag 13861 tgtagactgc agtggtgatg aaccttgaaa atatattaat agaagccaga cacgtaagtc 13921 acaaatggta taactcaatt tatatgaaat tcccagagag tccaaagtta aaaaaaacaa 13981 aaaggcagat tattggttgt cagtagctac agagatggga cattgaagat attaataggt 14041 atagggttga ggattcatgg cctttccctc ttccatgtta gcatgtctat tgttgttaac 14101 cttattcaac tcatgttagg gcaatcatat tgatgagact taatgagtat agcttgacat 14161 tcctagaaga cagagtctca catcagactc tctgatcctc tgactcttag aatctttcaa 14221 caccctcttc tgaaatgatc tctgagcctt tggttgtgac tgaattatga gaaagaattt 14281 tatgttgaat gaattttact tcagcatttt tacaaaactg aagctgggga tggcgtttca 14341 tgcgtgcagt ccctaccatt tgcctataag cctggaggag gcagaagtag aattaggctt 14401 ttcagggaca tcttcagctg catcatgagc ttgaggacaa cttgagctac ataagattcc 14461 catccccacc cccacacaca cacacacatc tagtatgtac ctagtatgca gtcttttcat 14521 atgtttaaaa tgtagaaagg tatatgtgaa gtctcccttt tccccacaac ttagtccctc 14581 caaagtattg catcagtttc cttgtacatg attttatgca tatataagat acactatttc 14641 tctctctctc tctctccttc cctccttccc tccaacctct gggtatgagt tgagggttat 14701 gtatgtttca agttgtaata agaattgcca ctcaaacatt tcatcccatt ttaagttaag 14761 tttgtctgat tttccacttt agaagggcag ccatgatggc tcatgcctat aatcccatcc 14821 tagcacttgt aagcctaata cataagactg gaggattgtc atcagcttga ggctagcctg 14881 gtctacctaa tgagttccag ccagccaaga gtagaatata agaccctgtc tccaaaaaat 14941 gtaccaaagg ggctggaggg gtaactcagt ggttaagaac agtagctgct cttccagagg 15001 acctgggttc aattcccagc acccacatgg cagcttgcaa ctatctgtaa ctgtccagct 15061 ttgggatatc cgacaccatt ataccaatta cataaaatga aaaaattgta aaatgtgcta 15121 aaatgagtgg ggagggggag catggtgtgg ttctgtgggc ttgggatttt gccatgaatc 15181 catgactata tctcagcaat atgtggatta tttctggaaa cctgtggggc atgggtgtat 15241 gggaagaggg gagagagaga gagcccgcat ccagccagag ttcctgtgct ctgggcaggc 15301 ggacgtggga ggactgctgg acgatttcca cttggccctg ggtgggcatc tggctgtgtg 15361 aagccactga ccccactcag cggggggtag ataaggggca tttcccatac caggttctca 15421 gactccagca ttccacgctg gatacagcag aagagctgag aacagaatag gggccccagg 15481 gagacactct cgtcccagag ataaagaagg cagagggaga gagattccta cgcaggcgag 15541 aggccttagt ctgggccttg atgaacagag acggtctatg gttttagagc tttattgtag 15601 aaaggcaggg ggaaagagag aaggtagaaa gagagagacc agccatggcc aagaggagag 15661 aaggggggaa aggagagaga gaagaaaggc tagagagtaa gaaaggttaa agagacagaa 15721 gtaaaagctt agagtaagag agagagagag agcaagaggg agagtaagaa aagtaaaagc 15781 ttaagagagc gaggaggggc cagcccctct aatagtgggc ttgttatctt gttgttgcta 15841 ggtaactgtg aagcgtctag cctgaaggcc agaagcttgg gacattgtct atgtgactga 15901 aagccatgag cctctactgt gggggctgtg gaggtggtaa cttggacagg agccaggggt 15961 tccaggagac atgagggaat gccttctgtc ccatgtaggt aggaattacc accaccggct 16021 ttcaccgtag ttcactggag actgtttgta catagcccat tgccccacag aaaccaggca 16081 ctcactgttt catttgcata agaattagaa ggctttattt tggctgggat tggtgacttg 16141 ccccagaaat tccagctttt gggaagccga atcctggaga accaggagtt caaggtcatc 16201 ctcaggtacc tcatttgttc aagaccagcc tgtgctacca tgagaccttg tccctactcc 16261 cctcaacccc aaaacacgct gttttcattt tataggttgt actaaccatg gaggatggtc 16321 atttgccttt ctcttgattt cagaagcgta gcgcgggctt tgcaccatga tctagtgttt 16381 ccagataggc ttgcctctcc acagccaggc ttatcctgct tcagtcccat gtttggcaag 16441 ggcaacatat gactgaagaa cttgcatagc cttatggtgt atcatgactc ttagccccag 16501 tttataactg tctaacagct ggtggctttt gtgaaaaagg aaagcatcat ttgaactttt 16561 aacttttcag ctgtttgttt tgactttgca taaatactga gactagctag gaaatgagct 16621 tttgtttcat ttgctaaaaa tgattttttt ttcatgaggc aatttcaagc aatttgaatt 16681 tagcaatctc tacttgtctg aaaagaagtt attaattaaa aaaaaacaaa caaacaggaa 16741 cctattatgc tggctagtca catgaaagaa tccaaatgaa cacagtcaag gctgaatcaa 16801 cacatgaaat tattttctaa ttccccaaat tacatttatg ctaattagta tgtagtaaaa 16861 cattttaatt gtttccaatg ttaaacatta cagaaataat tttgatgttt tcctaataac 16921 ccagcctttg atcatgtttg catttatttg attgttgtca gtttattaaa taattaagta 16981 agagcaacaa gataaaatga cttcatgaga gtccttttct gtaacttaga acgtcagtag 17041 tcataggaac tgcggtcgtt cccttacctt tcagaagtag gctaaagagt tgaacgcaaa 17101 ggtggttttt cgaatcttcc tgatccttaa gcccccacta ggtttatatt ctatatttga 17161 tatcggattc gaacccctcg agcttaggat ccggaaccct taatataact aagttattag 17221 gtccctcgaa gaggttcact agtactggcc attgcggccg cttcgaggct gcctcgcgcg 17281 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 17341 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 17401 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 17461 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 17521 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 17581 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 17641 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 17701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 17761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 17821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 17881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 17941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 18001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 18061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 18121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 18181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 18241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 18301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 18361 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 18421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 18481 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 18541 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 18601 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 18661 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 18721 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 18781 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 18841 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 18901 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 18961 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 19021 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 19081 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga 19141 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 19201 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 19261 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 19321 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 19381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 19441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgg //