LOCUS Ple88\(pEMS1375) 18678 bp DNA circular 29-APR-2008 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2364 - GCGTATCACGAGGCCCTTTC Tm=56.0 Deg C Antisense primer: oEMS2731 - GCTCAAGTGATCCTTCCACATCAG Tm=56.6 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 290bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|480858506| COMMENT VNTDBDATE|480858506| COMMENT LSOWNER| COMMENT VNTNAME|Ple88 (pEMS1375)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|George Yang| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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2 0 0 Nil -604 100) COMMENT (CObjectList COMMENT #182=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #0# 0) 1 2 0 0 #109# COMMENT 1667321128 100) (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 0.781209 COMMENT 2.06151 1 0.082322) 0.8 1.8 0) COMMENT #183=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #105# 1128481607 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "5' arm of Hprt homology" "@N" 1 -16.8739 COMMENT 1.74233 0 -10 11.2144 1.17389 #182#) COMMENT #184=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #3# 0) 1 2 0 0 #109# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.54716 4.50253 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #185=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #105# 20883460 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "3' arm of Hprt homology" "@N" 1 11.4827 COMMENT -10.454 0 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COMMENT #192=(AnlzFontBackColorItem 322 2367 40304639) COMMENT #193=(AnlzFontBackColorItem 298 321 43633766) COMMENT #194=(AnlzFontBackColorItem 53 72 43633766))) (CObList) 543453793 COMMENT (CStringList) 1380863812 825242196 (CObList))) FEATURES Location/Qualifiers misc_recomb 12541..16352 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker 17672..18672 /vntifkey="22" /label=AmpR misc_marker 11090..12515 /vntifkey="22" /label=AmpR misc_recomb 5273..11094 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 3495..5272 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 12522..12539 /vntifkey="21" /label=I-Sce\I misc_feature 2377..2410 /vntifkey="21" /label=FRT\(F5mutant) misc_feature 2411..2421 /vntifkey="21" /label=Kozak\consensus misc_feature 2418..3137 /vntifkey="21" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 3138..3173 /vntifkey="21" /label=Tetracysteine\residue misc_feature 3174..3246 /vntifkey="21" /label=NLS misc_feature 3252..3285 /vntifkey="21" /label=FRT misc_feature 3439..3489 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..2368 /vntifkey="21" /label=GFAP-A /note="pEMS1306" primer 299..322 /vntifkey="27" /label=oEMS2731 /note="antisense primer for GFAP-A MiniP construct" primer 54..73 /vntifkey="27" /label=oEMS2364 /note="Sense primer located in vector backbone" misc_feature 139..2368 /vntifkey="21" /label=LongProm\region BASE COUNT 4938 a 4296 c 4330 g 5114 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccca gatgtgacta gagcctaagg agctcccacc tccctctctg 181 tgctgggact cacagaggga gacctcagga ggcagtctgt ccatcacatg tccaaatgca 241 gagcataccc tgggctgggc gcagtggcgc acaactgtaa ttccagcact ttgggaggct 301 gatgtggaag gatcacttga gcccagaagt tctagaccag cctgggcaac atggcaagac 361 cctatctcta caaaaaaagt taaaaaatca gccacgtgtg gtgacacaca cctgtagtcc 421 cagctattca ggaggctgag gtgaggggat cacttaaggc tgggaggttg aggctgcagt 481 gagtcgtggt tgcgccactg cactccagcc tgggcaacag tgagaccctg tctcaaaaga 541 caaaaaaaaa aaaaaaaaaa aaaagaacat atcctggtgt ggagtagggg acgctgctct 601 gacagaggct cgggggcctg agctggctct gtgagctggg gaggaggcag acagccaggc 661 cttgtctgca agcagacctg gcagcattgg gctggccgcc ccccagggcc tcctcttcat 721 gcccagtgaa tgactcacct tggcacagac acaatgttcg gggtgggcac agtgcctgct 781 tcccgccgca ccccagcccc cctcaaatgc cttccgagaa gcccattgag cagggggctt 841 gcattgcacc ccagcctgac agcctggcat cttgggataa aagcagcaca gccccctagg 901 ggctgccctt gctgtgtggc gccaccggcg gtggagaaca aggctctatt cagcctgtgc 961 ccaggaaagg ggatcagggg atgcccaggc atggacagtg ggtggcaggg ggggagagga 1021 gggctgtctg cttcccagaa gtccaaggac acaaatgggt gaggggactg ggcagggttc 1081 tgaccctgtg ggaccagagt ggagggcgta gatggacctg aagtctccag ggacaacagg 1141 gcccaggtct caggctccta gttgggccca gtggctccag cgtttccaaa cccatccatc 1201 cccagaggtt cttcccatct ctccaggctg atgtgtggga actcgaggaa ataaatctcc 1261 agtgggagac ggaggggtgg ccagggaaac ggggcgctgc aggaataaag acgagccagc 1321 acagccagct catgtgtaac ggctttgtgg agctgtcaag gcctggtctc tgggagagag 1381 gcacagggag gccagacaag gaaggggtga cctggaggga cagatccagg ggctaaagtc 1441 ctgataaggc aagagagtgc cggccccctc ttgccctatc aggacctcca ctgccacata 1501 gaggccatga ttgaccctta gacaaagggc tggtgtccaa tcccagcccc cagccccaga 1561 actccaggga atgaatgggc agagagcagg aatgtgggac atctgtgttc aagggaagga 1621 ctccaggagt ctgctgggaa tgaggcctag taggaaatga ggtggccctt gagggtacag 1681 aacaggttca ttcttcgcca aattcccagc accttgcagg cacttacagc tgagtgagat 1741 aatgcctggg ttatgaaatc aaaaagttgg aaagcaggtc agaggtcatc tggtacagcc 1801 cttccttccc tttttttttt ttttttttgt gagacaaggt ctctctctgt tgcccaggct 1861 ggagtggcgc aaacacagct cactgcagcc tcaacctact gggctcaagc aatcctccag 1921 cctcagcctc ccaaagtgct gggattacaa gcatgagcca ccccactcag ccctttcctt 1981 cctttttaat tgatgcataa taattgtaag tattcatcat ggtccaacca accctttctt 2041 gacccacctt cctagagaga gggtcctctt gcttcagcgg tcagggcccc agacccatgg 2101 tctggctcca ggtaccacct gcctcatgca ggagttggcg tgcccaggaa gctctgcctc 2161 tgggcacagt gacctcagtg gggtgagggg agctctcccc atagctgggc tgcggcccaa 2221 ccccaccccc tcaggctatg ccagggggtg ttgccagggg cacccgggca tcgccagtct 2281 agcccactcc ttcataaagc cctcgcatcc caggagcgag cagagccaga gcaggttgga 2341 gaggagacgc atcacctccg ctgctcgcgg cgcgccgaag ttcctattct tcaaaaggta 2401 taggaacttc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 2461 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 2521 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 2581 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct 2641 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 2701 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 2761 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 2821 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 2881 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 2941 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 3001 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga 3061 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 3121 acgagctgta caagttattt ctgaactgct gcccgggctg ctgcatggaa ccggatccaa 3181 aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa 3241 aggtaatata agaagttcct attctctaga aagtatagga acttcagcgg ccgcgactct 3301 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 3361 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 3421 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 3481 tttcactgcg aattctcgta aaactcttca tggcagtagt tattattctc tctctctctc 3541 tttttctttt ttcttgagac aggatatttc tctgttgccc aggctggagt gcagtggcac 3601 agtcttggct cactgcagcc tggacctcct gggctcaagc catcctccca cctcagcctc 3661 ccaagtagct ggggctacag gcacatggcc accaggccca gataattttt catttttgta 3721 gagactgagt ctcaccatgt tacccaggtt tattattctc attttttaga tgaagagact 3781 gaggtccaga gaagctcaat gacttgccta gttttacaaa tctcctgcca tcacataccc 3841 ctcagcgtcc ttaataagag ggaggccacc aactatgtgc tgggcactgt ggtggatgct 3901 ggagctatag ggttgagtat ataagaaatg gtgttgctgg agcaactgtt gcttgcttac 3961 ctgacctatc tgagaattaa ttagcagggg aacatatttt tgttttcaga ttcaatataa 4021 gaacttgtgt gggcaaaaat aaagatcagt agtaataaca gtagttccca tttgctgact 4081 gtactgtcct aagtgcatat atatatacat acacacacgc atacctatac tcctctaata 4141 ctcaaaatga tcctgtttat gtattgttaa tatgctcatt ttatttttaa atttttattt 4201 atttttattt ttatttattt ttgagacgga gtctcattct gtcgcggagg ctgaagtgca 4261 gtggtgcgat ctcagctcag tgcgacctcc gcctcccggg ttcaagtgat tctcctgcct 4321 cagcctccgg actagctggg attacaggcg cccgcctcca cgcccagcta atttttgtat 4381 ttttagtaga gatggggttt cgccatgttg gccaggctgg tctcgtactc ctgaccttga 4441 gtgatccacc tgcctcggcc tcccaaagtg ctgggattac aggcatgagc caccgcgccg 4501 ggctaatatg ctcattttag tgaggcaaaa atagaggctc agagtctgat ttgtacaaaa 4561 ctacagagca gttaagtgtc ctctcagatg tgtaccctga tctgggtgac tctaggactc 4621 taggtctcaa ctgttacaac cagttaaggg tttggggaag cactgggcca agagtcagga 4681 aaatggaagc cacaggtagt gcaaggtctt gggaatggga cgtctggtcc aaggattcac 4741 gcgatgactg gaacccgaag agccggggcc cggtttacgg ccgccatgaa gcaacgcgcg 4801 ccggtaggtt tgggaatcag ggagccctct gaataggaga ctgagttggg agggaaaggg 4861 gcttcgctgg gggagcctcg gcttcttctg ggagaaaatt cccacggcta cctagtgagc 4921 ctgcaaactg gtaggcgccg gcgtaggcgc gcgggcgggg ccgggggcgg ggcctgcggg 4981 gcgtggcggg gcgggcagag ggcggggcct gcttctcctc agcttcaggc ggctgcgacg 5041 agccctcagg cgaacctctc ggctttcccg cgcggcgccg cctcttgctg cgcctccgcc 5101 tcctcctctg ctccgccacc ggcttcctcc tcctgagcag tcagcccgcg cgccggccgg 5161 ctccgttatg gcgacccgca gccctggcgt cgtggtgagc agctcggcct gccggccctg 5221 gccggttcag gcccacgcgg caggtggcgg ccgggccctg aggcgcggga tcgatccatg 5281 acaccccccc gccccccata tctcactata attcactgtc aaatggcttt tatgatttgg 5341 agtttgggga tacttttaaa gacagggtct tggtagctcc tagatggctt gggattcact 5401 tagtaaccct tgctggccta aaattcagga acttctacct catcttccca agtactgggt 5461 tagtagggat gtccagcata tttagcttgc tttaatgatt ttgctgtact tttttctgtg 5521 tgtacaggtg catgtatatt tataaaacta acataatata ttttaccccc aaagtaccat 5581 aaccctaatt aatgctatta aatgttttgt atgctgggct ctgggcatac taatataacc 5641 aatattatac aatgcaaaac aattttggaa tatcttctgt aaaattatct tattagtaag 5701 ttgtaaccat ctcagacttc taagtttgag tttgtaaagg atcagttttt tttggtgacc 5761 tacacacaca cacacacaca cacacacaca cacacacaca ggggggtgga ttctgagaat 5821 ttagagaatt tagatggtta ataccagttt ttatttttat tctgtgtgat atttatttat 5881 taagatttat ttatttatta catgtaggta cactgtatct gtcttcagac acaccagaag 5941 agggcatcag atctcgttac agatggttgt gagtcaccat gtggttgctg ggatttgaac 6001 tcaggacctc tggaagaaca gtcagtgctc ttaaccgttg agccatctca ccagccccgt 6061 gatattttat ttaacttact agaatatact gtgttaaaac atattttagg ggattgggga 6121 gatggcccat tctgtaaagt gtctgccatg tcatcttcag gacctgcgtt tgtaacccca 6181 gcatggagaa gacagagaca gggagacccc tgggcttcct ggcaagctag actagcaaat 6241 ttggttaact aggttcaatg agagactcag tctcaaaaca taagaagtga attttttaga 6301 cccaatttca atctctgaac tccatacgta tgcacatgta cctgcatata aatatatata 6361 cacacccata aacacatgca cacacaaaaa atggttcaaa agttcttaaa atgtataaga 6421 gtagaaagtg tagagccggg agtggtggcg cacgccttta atcccagcac ttgggagcag 6481 aggcaggtgg atttctgagt tcgaggccag cctggtctac agagtgagtt ccaggacagc 6541 cagagctata cagagaaatc ctgcctcaaa aaaccaaaaa taaagtgtat atactaatct 6601 ttcatgccta aaatatttat ttttgccaca tttttcatac ctctttaata tttttctttt 6661 tctctttgtg cttcaaagga aattttgtgt catgttttac ttttcattct tgactctatt 6721 cagcagtaag acgcagcatg gctttcttag agaagtataa tctattacaa aacggtaaca 6781 tttaactgct ctacataccc tcttgtagct aaattcctct gttaaactaa gacttattat 6841 ctaaacgtaa ttaaatgttg atatcttata atgctccact ttgaaacagc tgtctttctt 6901 ttgcagatta gcgatgatga accaggttat gacctagatt tgttttgtat acctaatcat 6961 tatgccgagg atttggaaaa agtgtttatt cctcatggac tgattatgga caggttagta 7021 agacctcgat tgaagttatt tttagagggt gtgtgtgtgg tgtgtgtgtg tgtgtgtgtg 7081 tgtgtgtgtg tgtgtgtgta agagagagag gagaggcatg tgtgatatat atgtgaagtg 7141 tattgcatgt acatggaatc cagagaagga cattaggtgt cctactctgt tgctctctta 7201 ttcccttgaa tcagtgtctc ttgctgaacc tggggcatac atactgattt ggctaggctg 7261 cctggctagc aagacccagg gatctgcttg tctctgcccc ccagtgctga cgttacaagc 7321 aggtgcaacc acatctggtt tttatatggg tactggggga tctgaactta gggcctgatg 7381 cgtgcccaag tgctctcacc cgccaagcca cctctccagc caaagggttt tttttcttgt 7441 attaattcta acttagcaac tgttgagtag ttgatgtttg aatataagct aagccaactt 7501 attagaataa atgttttcta ttttttactt gtatgtgtga gtgcctgcat gtatgtatgt 7561 gaagcatatg tattccagga tgtcagaatt gtgatggatt ccctggaact ggagttacag 7621 accattgtga accactgtgt atgggtgctg ggaactgaac tttaggtcct ctgcaaaaac 7681 agtaaggatc ttaactgata aactatcttt ctagccctaa ggctttctac ttttaaagtt 7741 cgtttctctt tctctctctc tctctctctc tctctctctc tctccctctc cctccctcct 7801 cctcctcctc ctctttcccc cctctcgcct cctctttctc tctcccctcc ctctctctct 7861 ctcacacaca cacacagaca gagagaggag agagaaagga agccttacta attaattacc 7921 tagttttaga cataactagt gggaatccat agtaagtaaa tcaacattct tgaagagagg 7981 ttagaagaat tggctctcgt tgtccatcga ggctgtataa gattattctt ctgttttctc 8041 agcttaccat acatattttc tacgtagaca ataccaattc aaatacactg tacgtaaagt 8101 atatgcgcac acacacacac acacacacac acacacacac acaagttaca tattgtcata 8161 tagtactaaa atttatggac tttttcatat taatacatct agagctatca tgttttttat 8221 cttttaaaaa ttactttacg gggctacaga gatgtctcag cagttaggag cactgactgc 8281 tcttccagag gtcctgagtt caattcccag caaccacatg gtggctcaca accatctgta 8341 atgggatctg atccactctt atggtgtgtc tgaagacagt gacagtgtac tcatatacat 8401 gaaataaatc tttaaaaaat tactttacca tcagggtata tatatatata tatatgtgtg 8461 tgtgtgtgtg tgtgtgtatg aagtatttgc tatttgagag ggtctcactg tttagctcag 8521 tctggcttgg aacgttctgt atagcccaga ctggcctcaa ctcagcagtc cccctgaaag 8581 gctctcatat gtggagatta caggtatgtg ctactgtgcc cagccctaca tgttactttg 8641 tttttgaaat agggtatcaa atagcttaga ctggcttcta agtcagtgtg tagctaagga 8701 tggtctgagc ttctgaacct gctacttcaa ctcctggtgt gccatcatgg ccagtttgca 8761 cagttgtgag agtagaactc aggacctcct atgtgttact agacatgttc tctaccagct 8821 gagctacatc ccaattcgcc tacatttact tttatttttt aaaagattta tttatgtata 8881 cagcactctg cctgcatgta catctgcatg ccagaagagg gcaccagatc tcatcataca 8941 tagttgtgag ccaccatgtg gttgctgggg attgaactca gggcctctgg aagagcagtc 9001 agtgctctta acctctgagc catctctgta gccccctaca tttattttaa agattatagg 9061 ctattctatt catagatgtg ccataactga tttaaccaga tcttcattat tttgtactta 9121 gtagagtata ttagagggaa tgaatcttaa atacacattt tgacacttgc tcagctttag 9181 acaagtaaaa atcactggtc aaggaaatgg tgcttagtgg atgcttctga actgtcaaat 9241 tgtcctccaa caagttacat cattttatac ttccaacgtt atgtgagagt tcatttttct 9301 ttaggctctt ctagtatttt atatttgaga aataaaattt aaccaaaatt tgccaatcta 9361 gtaagcttgt ttttatttgt atctttaaat tttatgatag atgttatagt gtactctcct 9421 ctcccctccc ctcccctctc ctcctcagca ggatcttgtt atgtagccct aagggatcta 9481 atacttactc tgtagtccag gctgtccttc agctcatgag acacaccatc atgcttggca 9541 tgtttcttgt tctgaaaggt atctggtttt aattttggtt gagctggtgt gttggtgtat 9601 atctgtaatc tcagcacagg caagtagaga caaggattat gattacagtc cagcctgggc 9661 tatttagagg gattctcttt aatctttcct catgccccaa aatcttacct ttggtatatg 9721 aaaaatagtc tccacttctg caaaatattg ctttatgaag taagaattaa ttcccttcat 9781 agagacaagg aatgtgtcct gtaaaagttt aatgtgtaag aagtatttgt tataaaagat 9841 aaatattcag aatcttcttt ttaattcctg attttatttc tataggactg aaagacttgc 9901 tcgagatgtc atgaaggaga tgggaggcca tcacattgtg gccctctgtg tgctcaaggg 9961 gggctataag ttctttgctg acctgctgga ttacattaaa gcactgaata gaaatagtga 10021 tagatccatt cctatgactg tagattttat cagactgaag agctactgtg taagtataat 10081 taacttataa ttaaaaaaat agggccattc tagttttatc tatatttttt ttaaacttgt 10141 gcaaactatg ctacataatc aacttacttg attatgtaat atatatagta atagttggaa 10201 ttactattat tacatattta tttttctgtg ctggggtaga acccagggaa ctaagtactg 10261 taataaccga gcttcattca tcctagtcat ttttaatttt tttttttttt tttgtttttt 10321 cgagacaagg tttctctgtg tagccctggc tgtcctggaa ctcactctgt agaccaggct 10381 ggcctcgaac tcagaaatct gcctgcctct gcctcccagt gctgagatta atgacgtgca 10441 ccaccactgc ctggcatttt tttaaagttt taatttgaga ccctttctca ctaacttgct 10501 cacactatcc tcgaactcat tacgtgggct ggtctagaaa tttcagtcaa cttgcctcag 10561 cttctcaagt agctgggatt acaggcaggt gctaccactt ctggccatga tcattgttgt 10621 tgttgctatt attattatta ttatttcact gatacttcat atcacaacac agaatagtaa 10681 ctgtagttca tgaccaacaa aaaagttgta cagtttctct gttgtcttat tttgatctgg 10741 attttgattt gtttcatggc actgggcagg ctaatctcaa actgcatggc tcaaatagtc 10801 cttctgtctt aatctcccat gttctggaag tataggtatc actacaccta gctgtatttt 10861 catattttaa aaaaaacgcc taagaattgc tattgaatga ggcggagaga tggctcagca 10921 gtcacaagta cttgtagagg actggggttt ggttactagc acctcaaaga tggcttacaa 10981 ttatctgtag ctccaattcc agaggatctg tgtggtaaag ctgcatgtat atgggcatgc 11041 aggtaaaact gctaaacata taaaaatagc tcagtcttaa gagaattggt cgactttttc 11101 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 11161 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 11221 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 11281 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 11341 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 11401 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 11461 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 11521 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 11581 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 11641 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 11701 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 11761 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 11821 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 11881 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 11941 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 12001 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 12061 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 12121 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 12181 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 12241 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 12301 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 12361 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 12421 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 12481 ctgctgcttg caaacaaaaa aaccaccgct accaggtcga gtagggataa cagggtaatg 12541 gatcctcgtt gggcacctgc actcatgtac gcaggcccac atatagatgt acacacgcat 12601 atcattcaaa atagaaatta tttatacaag tagtgcgttg gagagctggc tcaggagttg 12661 ttaatgcttg ctacccttgc aaaggaccca agttcaattt ccagcacctg tgttgggcat 12721 ctcacaacca catgtaaatc tagtttcagg ggatttcacc cctcttctga actcttcagg 12781 cacttatacc cacaaatgga catctccaca tagagacaca tacttttaaa aaagtaatct 12841 agggtctaga gaaatggtta tagaccacgg actgggatca gttcccagaa gccacatcct 12901 gtggctcaca attgcctgtg aatcaagttc tagatctggt ggcctcgtct agcctgcagt 12961 catcaacaaa cacatgcaca catccataca cacataattt aaaagaaagc gaaagtaacc 13021 tataatgcta aacaaagcac attctcagtg tagactgcag tggtgatgaa ccttgaaaat 13081 atattaatag aagccagaca cgtaagtcac aaatggtata actcaattta tatgaaattc 13141 ccagagagtc caaagttaaa aaaaacaaaa aggcagatta ttggttgtca gtagctacag 13201 agatgggaca ttgaagatat taataggtat agggttgagg attcatggcc tttccctctt 13261 ccatgttagc atgtctattg ttgttaacct tattcaactc atgttagggc aatcatattg 13321 atgagactta atgagtatag cttgacattc ctagaagaca gagtctcaca tcagactctc 13381 tgatcctctg actcttagaa tctttcaaca ccctcttctg aaatgatctc tgagcctttg 13441 gttgtgactg aattatgaga aagaatttta tgttgaatga attttacttc agcattttta 13501 caaaactgaa gctggggatg gcgtttcatg cgtgcagtcc ctaccatttg cctataagcc 13561 tggaggaggc agaagtagaa ttaggctttt cagggacatc ttcagctgca tcatgagctt 13621 gaggacaact tgagctacat aagattccca tccccacccc cacacacaca cacacatcta 13681 gtatgtacct agtatgcagt cttttcatat gtttaaaatg tagaaaggta tatgtgaagt 13741 ctcccttttc cccacaactt agtccctcca aagtattgca tcagtttcct tgtacatgat 13801 tttatgcata tataagatac actatttctc tctctctctc tctccttccc tccttccctc 13861 caacctctgg gtatgagttg agggttatgt atgtttcaag ttgtaataag aattgccact 13921 caaacatttc atcccatttt aagttaagtt tgtctgattt tccactttag aagggcagcc 13981 atgatggctc atgcctataa tcccatccta gcacttgtaa gcctaataca taagactgga 14041 ggattgtcat cagcttgagg ctagcctggt ctacctaatg agttccagcc agccaagagt 14101 agaatataag accctgtctc caaaaaatgt accaaagggg ctggaggggt aactcagtgg 14161 ttaagaacag tagctgctct tccagaggac ctgggttcaa ttcccagcac ccacatggca 14221 gcttgcaact atctgtaact gtccagcttt gggatatccg acaccattat accaattaca 14281 taaaatgaaa aaattgtaaa atgtgctaaa atgagtgggg agggggagca tggtgtggtt 14341 ctgtgggctt gggattttgc catgaatcca tgactatatc tcagcaatat gtggattatt 14401 tctggaaacc tgtggggcat gggtgtatgg gaagagggga gagagagaga gcccgcatcc 14461 agccagagtt cctgtgctct gggcaggcgg acgtgggagg actgctggac gatttccact 14521 tggccctggg tgggcatctg gctgtgtgaa gccactgacc ccactcagcg gggggtagat 14581 aaggggcatt tcccatacca ggttctcaga ctccagcatt ccacgctgga tacagcagaa 14641 gagctgagaa cagaataggg gccccaggga gacactctcg tcccagagat aaagaaggca 14701 gagggagaga gattcctacg caggcgagag gccttagtct gggccttgat gaacagagac 14761 ggtctatggt tttagagctt tattgtagaa aggcaggggg aaagagagaa ggtagaaaga 14821 gagagaccag ccatggccaa gaggagagaa ggggggaaag gagagagaga agaaaggcta 14881 gagagtaaga aaggttaaag agacagaagt aaaagcttag agtaagagag agagagagag 14941 caagagggag agtaagaaaa gtaaaagctt aagagagcga ggaggggcca gcccctctaa 15001 tagtgggctt gttatcttgt tgttgctagg taactgtgaa gcgtctagcc tgaaggccag 15061 aagcttggga cattgtctat gtgactgaaa gccatgagcc tctactgtgg gggctgtgga 15121 ggtggtaact tggacaggag ccaggggttc caggagacat gagggaatgc cttctgtccc 15181 atgtaggtag gaattaccac caccggcttt caccgtagtt cactggagac tgtttgtaca 15241 tagcccattg ccccacagaa accaggcact cactgtttca tttgcataag aattagaagg 15301 ctttattttg gctgggattg gtgacttgcc ccagaaattc cagcttttgg gaagccgaat 15361 cctggagaac caggagttca aggtcatcct caggtacctc atttgttcaa gaccagcctg 15421 tgctaccatg agaccttgtc cctactcccc tcaaccccaa aacacgctgt tttcatttta 15481 taggttgtac taaccatgga ggatggtcat ttgcctttct cttgatttca gaagcgtagc 15541 gcgggctttg caccatgatc tagtgtttcc agataggctt gcctctccac agccaggctt 15601 atcctgcttc agtcccatgt ttggcaaggg caacatatga ctgaagaact tgcatagcct 15661 tatggtgtat catgactctt agccccagtt tataactgtc taacagctgg tggcttttgt 15721 gaaaaaggaa agcatcattt gaacttttaa cttttcagct gtttgttttg actttgcata 15781 aatactgaga ctagctagga aatgagcttt tgtttcattt gctaaaaatg attttttttt 15841 catgaggcaa tttcaagcaa tttgaattta gcaatctcta cttgtctgaa aagaagttat 15901 taattaaaaa aaaacaaaca aacaggaacc tattatgctg gctagtcaca tgaaagaatc 15961 caaatgaaca cagtcaaggc tgaatcaaca catgaaatta ttttctaatt ccccaaatta 16021 catttatgct aattagtatg tagtaaaaca ttttaattgt ttccaatgtt aaacattaca 16081 gaaataattt tgatgttttc ctaataaccc agcctttgat catgtttgca tttatttgat 16141 tgttgtcagt ttattaaata attaagtaag agcaacaaga taaaatgact tcatgagagt 16201 ccttttctgt aacttagaac gtcagtagtc ataggaactg cggtcgttcc cttacctttc 16261 agaagtaggc taaagagttg aacgcaaagg tggtttttcg aatcttcctg atccttaagc 16321 ccccactagg tttatattct atatttgata tcggattcga acccctcgag cttaggatcc 16381 ggaaccctta atataactaa gttattaggt ccctcgaaga ggttcactag tactggccat 16441 tgcggccgct tcgaggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 16501 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 16561 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 16621 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 16681 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 16741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 16801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 16861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 16921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 16981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 17041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 17101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 17161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 17221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 17281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 17341 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 17401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 17461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 17521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 17581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 17641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 17701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 17761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 17821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 17881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 17941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc 18001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 18061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 18121 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 18181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 18241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac 18301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 18361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 18421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 18481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 18541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 18601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 18661 aaaagtgcca cctgacgg //