LOCUS pEMS1157_Public 18070 bp DNA circular 10-MAR-2008 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2364 - GCGTATCACGAGGCCCTTTC Tm=56.0 Deg C Antisense primer: oEMS2668 - GCCAAGTAGGAAAGTCCCATAAGG Tm=56.3 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 370 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|471457386| COMMENT VNTDBDATE|476443582| COMMENT LSOWNER| COMMENT VNTNAME|pEMS1157_Public| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|George Yang| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #198=(AnlzFontBackColorItem 53 72 43633766) COMMENT #199=(AnlzFontBackColorItem 378 401 43633766))) (CObList) 1953066364 COMMENT (CStringList) 1869112175 1769104494 (CObList))) FEATURES Location/Qualifiers misc_recomb 11933..15744 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker 17064..18064 /vntifkey="22" /label=AmpR misc_marker 10482..11907 /vntifkey="22" /label=AmpR misc_recomb 4665..10486 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 2887..4664 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 11914..11931 /vntifkey="21" /label=I-Sce\I misc_feature 1769..1802 /vntifkey="21" /label=FRT\(F5mutant) misc_feature 1803..1813 /vntifkey="21" /label=Kozak\consensus misc_feature 1810..2529 /vntifkey="21" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 2530..2565 /vntifkey="21" /label=Tetracysteine\residue misc_feature 2566..2638 /vntifkey="21" /label=NLS misc_feature 2644..2677 /vntifkey="21" /label=FRT misc_feature 2831..2881 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 128..1754 /vntifkey="21" /label=CAG primer 54..73 /vntifkey="27" /label=oEMS2364 primer 379..402 /vntifkey="27" /label=oEMS2668 BASE COUNT 4627 a 4158 c 4292 g 4993 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctctgc aggcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 181 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 241 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 301 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 361 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 421 tcatcgctat taacatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc 481 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg 541 gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg 601 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt 661 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggga 721 gtcgctgcga cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt 961 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg 1021 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg 1081 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg 1141 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc 1201 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg 1261 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg 1321 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg 1381 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca 1441 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc 1501 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg 1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg 1621 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac 1681 cggcggctct agacaattgt actaaccttc ttctctttcc tctcctgaca ggttggtgta 1741 cagtagcttc caccacgcgt ggcgcgccga agttcctatt cttcaaaagg tataggaact 1801 tccgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 1861 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 1921 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 1981 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac 2041 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 2101 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 2161 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 2221 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 2281 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 2341 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 2401 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 2461 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 2521 tacaagttat ttctgaactg ctgcccgggc tgctgcatgg aaccggatcc aaaaaagaag 2581 agaaaggtag atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtaata 2641 taagaagttc ctattctcta gaaagtatag gaacttcagc ggccgcgact ctagatcata 2701 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 2761 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 2821 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 2881 cgaattctcg taaaactctt catggcagta gttattattc tctctctctc tctttttctt 2941 ttttcttgag acaggatatt tctctgttgc ccaggctgga gtgcagtggc acagtcttgg 3001 ctcactgcag cctggacctc ctgggctcaa gccatcctcc cacctcagcc tcccaagtag 3061 ctggggctac aggcacatgg ccaccaggcc cagataattt ttcatttttg tagagactga 3121 gtctcaccat gttacccagg tttattattc tcatttttta gatgaagaga ctgaggtcca 3181 gagaagctca atgacttgcc tagttttaca aatctcctgc catcacatac ccctcagcgt 3241 ccttaataag agggaggcca ccaactatgt gctgggcact gtggtggatg ctggagctat 3301 agggttgagt atataagaaa tggtgttgct ggagcaactg ttgcttgctt acctgaccta 3361 tctgagaatt aattagcagg ggaacatatt tttgttttca gattcaatat aagaacttgt 3421 gtgggcaaaa ataaagatca gtagtaataa cagtagttcc catttgctga ctgtactgtc 3481 ctaagtgcat atatatatac atacacacac gcatacctat actcctctaa tactcaaaat 3541 gatcctgttt atgtattgtt aatatgctca ttttattttt aaatttttat ttatttttat 3601 ttttatttat ttttgagacg gagtctcatt ctgtcgcgga ggctgaagtg cagtggtgcg 3661 atctcagctc agtgcgacct ccgcctcccg ggttcaagtg attctcctgc ctcagcctcc 3721 ggactagctg ggattacagg cgcccgcctc cacgcccagc taatttttgt atttttagta 3781 gagatggggt ttcgccatgt tggccaggct ggtctcgtac tcctgacctt gagtgatcca 3841 cctgcctcgg cctcccaaag tgctgggatt acaggcatga gccaccgcgc cgggctaata 3901 tgctcatttt agtgaggcaa aaatagaggc tcagagtctg atttgtacaa aactacagag 3961 cagttaagtg tcctctcaga tgtgtaccct gatctgggtg actctaggac tctaggtctc 4021 aactgttaca accagttaag ggtttgggga agcactgggc caagagtcag gaaaatggaa 4081 gccacaggta gtgcaaggtc ttgggaatgg gacgtctggt ccaaggattc acgcgatgac 4141 tggaacccga agagccgggg cccggtttac ggccgccatg aagcaacgcg cgccggtagg 4201 tttgggaatc agggagccct ctgaatagga gactgagttg ggagggaaag gggcttcgct 4261 gggggagcct cggcttcttc tgggagaaaa ttcccacggc tacctagtga gcctgcaaac 4321 tggtaggcgc cggcgtaggc gcgcgggcgg ggccgggggc ggggcctgcg gggcgtggcg 4381 gggcgggcag agggcggggc ctgcttctcc tcagcttcag gcggctgcga cgagccctca 4441 ggcgaacctc tcggctttcc cgcgcggcgc cgcctcttgc tgcgcctccg cctcctcctc 4501 tgctccgcca ccggcttcct cctcctgagc agtcagcccg cgcgccggcc ggctccgtta 4561 tggcgacccg cagccctggc gtcgtggtga gcagctcggc ctgccggccc tggccggttc 4621 aggcccacgc ggcaggtggc ggccgggccc tgaggcgcgg gatcgatcca tgacaccccc 4681 ccgcccccca tatctcacta taattcactg tcaaatggct tttatgattt ggagtttggg 4741 gatactttta aagacagggt cttggtagct cctagatggc ttgggattca cttagtaacc 4801 cttgctggcc taaaattcag gaacttctac ctcatcttcc caagtactgg gttagtaggg 4861 atgtccagca tatttagctt gctttaatga ttttgctgta cttttttctg tgtgtacagg 4921 tgcatgtata tttataaaac taacataata tattttaccc ccaaagtacc ataaccctaa 4981 ttaatgctat taaatgtttt gtatgctggg ctctgggcat actaatataa ccaatattat 5041 acaatgcaaa acaattttgg aatatcttct gtaaaattat cttattagta agttgtaacc 5101 atctcagact tctaagtttg agtttgtaaa ggatcagttt tttttggtga cctacacaca 5161 cacacacaca cacacacaca cacacacaca caggggggtg gattctgaga atttagagaa 5221 tttagatggt taataccagt ttttattttt attctgtgtg atatttattt attaagattt 5281 atttatttat tacatgtagg tacactgtat ctgtcttcag acacaccaga agagggcatc 5341 agatctcgtt acagatggtt gtgagtcacc atgtggttgc tgggatttga actcaggacc 5401 tctggaagaa cagtcagtgc tcttaaccgt tgagccatct caccagcccc gtgatatttt 5461 atttaactta ctagaatata ctgtgttaaa acatatttta ggggattggg gagatggccc 5521 attctgtaaa gtgtctgcca tgtcatcttc aggacctgcg tttgtaaccc cagcatggag 5581 aagacagaga cagggagacc cctgggcttc ctggcaagct agactagcaa atttggttaa 5641 ctaggttcaa tgagagactc agtctcaaaa cataagaagt gaatttttta gacccaattt 5701 caatctctga actccatacg tatgcacatg tacctgcata taaatatata tacacaccca 5761 taaacacatg cacacacaaa aaatggttca aaagttctta aaatgtataa gagtagaaag 5821 tgtagagccg ggagtggtgg cgcacgcctt taatcccagc acttgggagc agaggcaggt 5881 ggatttctga gttcgaggcc agcctggtct acagagtgag ttccaggaca gccagagcta 5941 tacagagaaa tcctgcctca aaaaaccaaa aataaagtgt atatactaat ctttcatgcc 6001 taaaatattt atttttgcca catttttcat acctctttaa tatttttctt tttctctttg 6061 tgcttcaaag gaaattttgt gtcatgtttt acttttcatt cttgactcta ttcagcagta 6121 agacgcagca tggctttctt agagaagtat aatctattac aaaacggtaa catttaactg 6181 ctctacatac cctcttgtag ctaaattcct ctgttaaact aagacttatt atctaaacgt 6241 aattaaatgt tgatatctta taatgctcca ctttgaaaca gctgtctttc ttttgcagat 6301 tagcgatgat gaaccaggtt atgacctaga tttgttttgt atacctaatc attatgccga 6361 ggatttggaa aaagtgttta ttcctcatgg actgattatg gacaggttag taagacctcg 6421 attgaagtta tttttagagg gtgtgtgtgt ggtgtgtgtg tgtgtgtgtg tgtgtgtgtg 6481 tgtgtgtgtg taagagagag aggagaggca tgtgtgatat atatgtgaag tgtattgcat 6541 gtacatggaa tccagagaag gacattaggt gtcctactct gttgctctct tattcccttg 6601 aatcagtgtc tcttgctgaa cctggggcat acatactgat ttggctaggc tgcctggcta 6661 gcaagaccca gggatctgct tgtctctgcc ccccagtgct gacgttacaa gcaggtgcaa 6721 ccacatctgg tttttatatg ggtactgggg gatctgaact tagggcctga tgcgtgccca 6781 agtgctctca cccgccaagc cacctctcca gccaaagggt tttttttctt gtattaattc 6841 taacttagca actgttgagt agttgatgtt tgaatataag ctaagccaac ttattagaat 6901 aaatgttttc tattttttac ttgtatgtgt gagtgcctgc atgtatgtat gtgaagcata 6961 tgtattccag gatgtcagaa ttgtgatgga ttccctggaa ctggagttac agaccattgt 7021 gaaccactgt gtatgggtgc tgggaactga actttaggtc ctctgcaaaa acagtaagga 7081 tcttaactga taaactatct ttctagccct aaggctttct acttttaaag ttcgtttctc 7141 tttctctctc tctctctctc tctctctctc tctctccctc tccctccctc ctcctcctcc 7201 tcctctttcc cccctctcgc ctcctctttc tctctcccct ccctctctct ctctcacaca 7261 cacacacaga cagagagagg agagagaaag gaagccttac taattaatta cctagtttta 7321 gacataacta gtgggaatcc atagtaagta aatcaacatt cttgaagaga ggttagaaga 7381 attggctctc gttgtccatc gaggctgtat aagattattc ttctgttttc tcagcttacc 7441 atacatattt tctacgtaga caataccaat tcaaatacac tgtacgtaaa gtatatgcgc 7501 acacacacac acacacacac acacacacac acacaagtta catattgtca tatagtacta 7561 aaatttatgg actttttcat attaatacat ctagagctat catgtttttt atcttttaaa 7621 aattacttta cggggctaca gagatgtctc agcagttagg agcactgact gctcttccag 7681 aggtcctgag ttcaattccc agcaaccaca tggtggctca caaccatctg taatgggatc 7741 tgatccactc ttatggtgtg tctgaagaca gtgacagtgt actcatatac atgaaataaa 7801 tctttaaaaa attactttac catcagggta tatatatata tatatatgtg tgtgtgtgtg 7861 tgtgtgtgta tgaagtattt gctatttgag agggtctcac tgtttagctc agtctggctt 7921 ggaacgttct gtatagccca gactggcctc aactcagcag tccccctgaa aggctctcat 7981 atgtggagat tacaggtatg tgctactgtg cccagcccta catgttactt tgtttttgaa 8041 atagggtatc aaatagctta gactggcttc taagtcagtg tgtagctaag gatggtctga 8101 gcttctgaac ctgctacttc aactcctggt gtgccatcat ggccagtttg cacagttgtg 8161 agagtagaac tcaggacctc ctatgtgtta ctagacatgt tctctaccag ctgagctaca 8221 tcccaattcg cctacattta cttttatttt ttaaaagatt tatttatgta tacagcactc 8281 tgcctgcatg tacatctgca tgccagaaga gggcaccaga tctcatcata catagttgtg 8341 agccaccatg tggttgctgg ggattgaact cagggcctct ggaagagcag tcagtgctct 8401 taacctctga gccatctctg tagcccccta catttatttt aaagattata ggctattcta 8461 ttcatagatg tgccataact gatttaacca gatcttcatt attttgtact tagtagagta 8521 tattagaggg aatgaatctt aaatacacat tttgacactt gctcagcttt agacaagtaa 8581 aaatcactgg tcaaggaaat ggtgcttagt ggatgcttct gaactgtcaa attgtcctcc 8641 aacaagttac atcattttat acttccaacg ttatgtgaga gttcattttt ctttaggctc 8701 ttctagtatt ttatatttga gaaataaaat ttaaccaaaa tttgccaatc tagtaagctt 8761 gtttttattt gtatctttaa attttatgat agatgttata gtgtactctc ctctcccctc 8821 ccctcccctc tcctcctcag caggatcttg ttatgtagcc ctaagggatc taatacttac 8881 tctgtagtcc aggctgtcct tcagctcatg agacacacca tcatgcttgg catgtttctt 8941 gttctgaaag gtatctggtt ttaattttgg ttgagctggt gtgttggtgt atatctgtaa 9001 tctcagcaca ggcaagtaga gacaaggatt atgattacag tccagcctgg gctatttaga 9061 gggattctct ttaatctttc ctcatgcccc aaaatcttac ctttggtata tgaaaaatag 9121 tctccacttc tgcaaaatat tgctttatga agtaagaatt aattcccttc atagagacaa 9181 ggaatgtgtc ctgtaaaagt ttaatgtgta agaagtattt gttataaaag ataaatattc 9241 agaatcttct ttttaattcc tgattttatt tctataggac tgaaagactt gctcgagatg 9301 tcatgaagga gatgggaggc catcacattg tggccctctg tgtgctcaag gggggctata 9361 agttctttgc tgacctgctg gattacatta aagcactgaa tagaaatagt gatagatcca 9421 ttcctatgac tgtagatttt atcagactga agagctactg tgtaagtata attaacttat 9481 aattaaaaaa atagggccat tctagtttta tctatatttt ttttaaactt gtgcaaacta 9541 tgctacataa tcaacttact tgattatgta atatatatag taatagttgg aattactatt 9601 attacatatt tatttttctg tgctggggta gaacccaggg aactaagtac tgtaataacc 9661 gagcttcatt catcctagtc atttttaatt tttttttttt tttttgtttt ttcgagacaa 9721 ggtttctctg tgtagccctg gctgtcctgg aactcactct gtagaccagg ctggcctcga 9781 actcagaaat ctgcctgcct ctgcctccca gtgctgagat taatgacgtg caccaccact 9841 gcctggcatt tttttaaagt tttaatttga gaccctttct cactaacttg ctcacactat 9901 cctcgaactc attacgtggg ctggtctaga aatttcagtc aacttgcctc agcttctcaa 9961 gtagctggga ttacaggcag gtgctaccac ttctggccat gatcattgtt gttgttgcta 10021 ttattattat tattatttca ctgatacttc atatcacaac acagaatagt aactgtagtt 10081 catgaccaac aaaaaagttg tacagtttct ctgttgtctt attttgatct ggattttgat 10141 ttgtttcatg gcactgggca ggctaatctc aaactgcatg gctcaaatag tccttctgtc 10201 ttaatctccc atgttctgga agtataggta tcactacacc tagctgtatt ttcatatttt 10261 aaaaaaaacg cctaagaatt gctattgaat gaggcggaga gatggctcag cagtcacaag 10321 tacttgtaga ggactggggt ttggttacta gcacctcaaa gatggcttac aattatctgt 10381 agctccaatt ccagaggatc tgtgtggtaa agctgcatgt atatgggcat gcaggtaaaa 10441 ctgctaaaca tataaaaata gctcagtctt aagagaattg gtcgactttt tcgccctttg 10501 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 10561 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 10621 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca 10681 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 10741 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 10801 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 10861 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 10921 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 10981 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 11041 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 11101 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 11161 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 11221 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 11281 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 11341 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 11401 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 11461 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 11521 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 11581 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 11641 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 11701 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 11761 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 11821 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 11881 tgcaaacaaa aaaaccaccg ctaccaggtc gagtagggat aacagggtaa tggatcctcg 11941 ttgggcacct gcactcatgt acgcaggccc acatatagat gtacacacgc atatcattca 12001 aaatagaaat tatttataca agtagtgcgt tggagagctg gctcaggagt tgttaatgct 12061 tgctaccctt gcaaaggacc caagttcaat ttccagcacc tgtgttgggc atctcacaac 12121 cacatgtaaa tctagtttca ggggatttca cccctcttct gaactcttca ggcacttata 12181 cccacaaatg gacatctcca catagagaca catactttta aaaaagtaat ctagggtcta 12241 gagaaatggt tatagaccac ggactgggat cagttcccag aagccacatc ctgtggctca 12301 caattgcctg tgaatcaagt tctagatctg gtggcctcgt ctagcctgca gtcatcaaca 12361 aacacatgca cacatccata cacacataat ttaaaagaaa gcgaaagtaa cctataatgc 12421 taaacaaagc acattctcag tgtagactgc agtggtgatg aaccttgaaa atatattaat 12481 agaagccaga cacgtaagtc acaaatggta taactcaatt tatatgaaat tcccagagag 12541 tccaaagtta aaaaaaacaa aaaggcagat tattggttgt cagtagctac agagatggga 12601 cattgaagat attaataggt atagggttga ggattcatgg cctttccctc ttccatgtta 12661 gcatgtctat tgttgttaac cttattcaac tcatgttagg gcaatcatat tgatgagact 12721 taatgagtat agcttgacat tcctagaaga cagagtctca catcagactc tctgatcctc 12781 tgactcttag aatctttcaa caccctcttc tgaaatgatc tctgagcctt tggttgtgac 12841 tgaattatga gaaagaattt tatgttgaat gaattttact tcagcatttt tacaaaactg 12901 aagctgggga tggcgtttca tgcgtgcagt ccctaccatt tgcctataag cctggaggag 12961 gcagaagtag aattaggctt ttcagggaca tcttcagctg catcatgagc ttgaggacaa 13021 cttgagctac ataagattcc catccccacc cccacacaca cacacacatc tagtatgtac 13081 ctagtatgca gtcttttcat atgtttaaaa tgtagaaagg tatatgtgaa gtctcccttt 13141 tccccacaac ttagtccctc caaagtattg catcagtttc cttgtacatg attttatgca 13201 tatataagat acactatttc tctctctctc tctctccttc cctccttccc tccaacctct 13261 gggtatgagt tgagggttat gtatgtttca agttgtaata agaattgcca ctcaaacatt 13321 tcatcccatt ttaagttaag tttgtctgat tttccacttt agaagggcag ccatgatggc 13381 tcatgcctat aatcccatcc tagcacttgt aagcctaata cataagactg gaggattgtc 13441 atcagcttga ggctagcctg gtctacctaa tgagttccag ccagccaaga gtagaatata 13501 agaccctgtc tccaaaaaat gtaccaaagg ggctggaggg gtaactcagt ggttaagaac 13561 agtagctgct cttccagagg acctgggttc aattcccagc acccacatgg cagcttgcaa 13621 ctatctgtaa ctgtccagct ttgggatatc cgacaccatt ataccaatta cataaaatga 13681 aaaaattgta aaatgtgcta aaatgagtgg ggagggggag catggtgtgg ttctgtgggc 13741 ttgggatttt gccatgaatc catgactata tctcagcaat atgtggatta tttctggaaa 13801 cctgtggggc atgggtgtat gggaagaggg gagagagaga gagcccgcat ccagccagag 13861 ttcctgtgct ctgggcaggc ggacgtggga ggactgctgg acgatttcca cttggccctg 13921 ggtgggcatc tggctgtgtg aagccactga ccccactcag cggggggtag ataaggggca 13981 tttcccatac caggttctca gactccagca ttccacgctg gatacagcag aagagctgag 14041 aacagaatag gggccccagg gagacactct cgtcccagag ataaagaagg cagagggaga 14101 gagattccta cgcaggcgag aggccttagt ctgggccttg atgaacagag acggtctatg 14161 gttttagagc tttattgtag aaaggcaggg ggaaagagag aaggtagaaa gagagagacc 14221 agccatggcc aagaggagag aaggggggaa aggagagaga gaagaaaggc tagagagtaa 14281 gaaaggttaa agagacagaa gtaaaagctt agagtaagag agagagagag agcaagaggg 14341 agagtaagaa aagtaaaagc ttaagagagc gaggaggggc cagcccctct aatagtgggc 14401 ttgttatctt gttgttgcta ggtaactgtg aagcgtctag cctgaaggcc agaagcttgg 14461 gacattgtct atgtgactga aagccatgag cctctactgt gggggctgtg gaggtggtaa 14521 cttggacagg agccaggggt tccaggagac atgagggaat gccttctgtc ccatgtaggt 14581 aggaattacc accaccggct ttcaccgtag ttcactggag actgtttgta catagcccat 14641 tgccccacag aaaccaggca ctcactgttt catttgcata agaattagaa ggctttattt 14701 tggctgggat tggtgacttg ccccagaaat tccagctttt gggaagccga atcctggaga 14761 accaggagtt caaggtcatc ctcaggtacc tcatttgttc aagaccagcc tgtgctacca 14821 tgagaccttg tccctactcc cctcaacccc aaaacacgct gttttcattt tataggttgt 14881 actaaccatg gaggatggtc atttgccttt ctcttgattt cagaagcgta gcgcgggctt 14941 tgcaccatga tctagtgttt ccagataggc ttgcctctcc acagccaggc ttatcctgct 15001 tcagtcccat gtttggcaag ggcaacatat gactgaagaa cttgcatagc cttatggtgt 15061 atcatgactc ttagccccag tttataactg tctaacagct ggtggctttt gtgaaaaagg 15121 aaagcatcat ttgaactttt aacttttcag ctgtttgttt tgactttgca taaatactga 15181 gactagctag gaaatgagct tttgtttcat ttgctaaaaa tgattttttt ttcatgaggc 15241 aatttcaagc aatttgaatt tagcaatctc tacttgtctg aaaagaagtt attaattaaa 15301 aaaaaacaaa caaacaggaa cctattatgc tggctagtca catgaaagaa tccaaatgaa 15361 cacagtcaag gctgaatcaa cacatgaaat tattttctaa ttccccaaat tacatttatg 15421 ctaattagta tgtagtaaaa cattttaatt gtttccaatg ttaaacatta cagaaataat 15481 tttgatgttt tcctaataac ccagcctttg atcatgtttg catttatttg attgttgtca 15541 gtttattaaa taattaagta agagcaacaa gataaaatga cttcatgaga gtccttttct 15601 gtaacttaga acgtcagtag tcataggaac tgcggtcgtt cccttacctt tcagaagtag 15661 gctaaagagt tgaacgcaaa ggtggttttt cgaatcttcc tgatccttaa gcccccacta 15721 ggtttatatt ctatatttga tatcggattc gaacccctcg agcttaggat ccggaaccct 15781 taatataact aagttattag gtccctcgaa gaggttcact agtactggcc attgcggccg 15841 cttcgaggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 15901 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 15961 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc 16021 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 16081 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg 16141 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 16201 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 16261 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 16321 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 16381 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 16441 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 16501 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 16561 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 16621 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 16681 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 16741 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 16801 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 16861 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 16921 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 16981 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 17041 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 17101 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 17161 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 17221 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 17281 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 17341 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg 17401 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 17461 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 17521 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 17581 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 17641 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata 17701 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 17761 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 17821 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 17881 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 17941 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 18001 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 18061 cacctgacgg //