LOCUS Ple99\(pEMS1071) 18935 bp DNA circular 8-OCT-2010 SOURCE ORGANISM COMMENT The sequence shown is the expected sequence. Sequence verification of actual construct was not available PCR genotyping: Sense primer: oEMS2349 - 5' CTGCTTTATGCCAGTTTCCCTAGG - 3' Tm=57.3 Deg C Antisense primer: oEMS2335 - 5' TGAGGGGTATGTGATGGCAGG - 3 Tm=56.9 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 777 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|567532136| COMMENT VNTDBDATE|567532136| COMMENT LSOWNER| COMMENT VNTNAME|Ple99 (pEMS1071)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|George Yang| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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0) 1 2 0 0 Nil -186 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #188=(AnlzFontBackColorItem 4059 4079 43633766) COMMENT #189=(AnlzFontBackColorItem 1976 2000 40304639))) (CObList) 1919252069 COMMENT (CStringList) 1114668146 1914717249 (CObList))) FEATURES Location/Qualifiers misc_recomb 12797..16608 /vntifkey="86" /label=5'\junction /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker 17928..18928 /vntifkey="22" /label=AmpR misc_marker 11325..12771 /vntifkey="22" /label=AmpR misc_recomb 8439..11329 /vntifkey="86" /label=HPRT\3'\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 3732..8438 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 12778..12795 /vntifkey="21" /label=I-Sce\I misc_feature complement(122..155) /vntifkey="21" /label=FRT misc_feature complement(2220..2253) /vntifkey="21" /label=FRT CDS complement(1191..2219) /vntifkey="4" /label=Cre misc_feature complement(441..1160) /vntifkey="21" /label=EGFP misc_feature complement(359..431) /vntifkey="21" /label=NLS misc_feature complement(156..206) /vntifkey="21" /label=SV40\early\mRNA\poly\A\signal misc_feature complement(2286..3703) /vntifkey="21" /label=GRP-A primer 4060..4080 /vntifkey="27" /label=oEMS2335 primer 3304..3327 /vntifkey="27" /label=oEMS2349 BASE COUNT 4818 a 4403 c 4278 g 5435 t 1 others ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgaagttcct atactttcta gagaatagga acttcgcagt gaaaaaaatg ctttatttgt 181 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 241 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 301 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc gcggccgctt 361 acctttctct tcttttttgg atctaccttt ctcttctttt ttggatctac ctttctcttc 421 ttttttggat cgcggccgct taacttgtac agctcgtcca tgccgagagt gatcccggcg 481 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg 541 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg 601 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc 661 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg 721 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc 781 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg 841 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg 901 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg 961 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg 1021 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac 1081 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc 1141 tcctcgccct tgctcaccat ggtggcgatg gatcccgggc ccgcggtacc atcgccatct 1201 tccagcaggc gcaccattgc ccctgtttca ctatccaggt tacggatata gttcatgaca 1261 atatttacat tggtccagcc accagcttgc atgatctccg gtattgaaac tccagcgcgg 1321 gccatatctc gcgcggctcc gacacgggca ctgtgtccag accaggccag gtatctctga 1381 ccagagtcat ccttagcgcc gtaaatcaat cgatgagttg cttcaaaaat cccttccagg 1441 gcgcgagttg atagctggct ggtggcagat ggcgcggcaa caccattttt tctgacccgg 1501 caaaacaggt agttattcgg atcatcagct acaccagaga cggaaatcca tcgctcgacc 1561 agtttagtta cccccaggct aagtgccttc tctacacctg cggtgctaac cagcgttttc 1621 gttctgccaa tatggattaa cattctccca ccgtcagtac gtgagatatc tttaaccctg 1681 atcctggcaa tttcggctat acgtaacagg gtgttataag caatccccag aaatgccaga 1741 ttacgtatat cctggcagcg atcgctattt tccatgagtg aacgaacctg gtcgaaatca 1801 gtgcgttcga acgctagagc ctgttttgca cgttcaccgg catcaacgtt ttcttttcgg 1861 atccgccgca taaccagtga aacagcattg ctgtcacttg gtcgtggcag cccggaccga 1921 cgatgaagca tgtttagctg gcccaaatgt tgctggatag tttttactgc cagaccgcgc 1981 gcctgaagat atagaagata atcgcgaaca tcttcaggtt ctgcgggaaa ccatttccgg 2041 ttattcaact tgcaccatgc cgcccacgac cggcaaacgg acagaagcat tttccaggta 2101 tgctcagaaa acgcctggcg atccctgaac atgtccatca ggttcttgcg aacctcatca 2161 ctcgttgcat cgaccggtaa tgcaggcaaa ttttggtgta cggtcagtaa attggacatg 2221 aagttcctat actttctaga gaataggaac ttcagatctg agtccggtag cgccacgtga 2281 cgcgtgacgg gaagcccttg gagcgcgccg gagaggctgg gaagagcaga gactggtgac 2341 ccggacctcg gaggagctcc gccgccgcag ccgctggtgc tactgctgcg gcctccacta 2401 gggcccctac tttatatgga agcccgggcg ggggggctgg ggggctcttc tatcgcggac 2461 cagagcgctc tgacgtctcc ctatagctaa ccggggcgct gggacgcccg gtccggaaaa 2521 ccatccatct ccccgccgag tgatgaaaag gctgagtgta cattgccccc tcttttttcc 2581 tcgtagcccc gtccgccttc cccacatcgg attaattcct ccggtcctcg acgccttcca 2641 tccaacacaa tttagtgaac tcctccctct ccaggctcgc cggcgcctcc agccttagct 2701 gccttgagag tcctggagag ggcgaccccg ctgcctgatc tgggccggga ggggtggggg 2761 gtgtagctgg agacgggtga gctctaaaga tggatgggct ttgctggagg gatttggaac 2821 caccatttga aggccttctg attctccaga cctttctctt cgtaggccac atctttgctg 2881 tcccagccca tagattttgg aaccttgcct ccttgcagat ctctctctct ttttctggac 2941 cttgcctctt tcttgcagat ctcctcccct cctctcccct cctctcccct cctctcccct 3001 cccttcccct cctctcccct cctctcccct cccttcccct cctctccact cccataccct 3061 cccctcccct ccactcccct tctctcttct cctccccttc ccatccctct ctctctcccc 3121 tcctccccaa tctctctctc tctctgtctc tctctccccc tctgctgccc tccaccttaa 3181 ggttaagggc agtatcctta acccacctgg tattcccagg cagctctgcc cacttgtatg 3241 gcaagacaga agatagctgg cccttttcac aagcatgcaa cttccatgga atagagaaag 3301 aaactgcttt atgccagttt ccctaggctg cctctacctg aaagctcaaa ctgtccctaa 3361 actgccacat acacacccct ctagagttgc tcaacgttcc tctgctcctt ctgttctcag 3421 atctaaagaa tggtcaccag cccaatcccc gaggttctaa ataccatgga tacaaagggg 3481 atcagcacca tagcaatgca acaataaata gaagctggta ggatgctgtt gcccacgttt 3541 tggctgccag agttggccgt gtggttggaa taggtgaggt ctattcctcc tcaaatgctt 3601 gttaattgcc tccctgttgg gtctttagtg ttccatgtca acatggtgtt gatattgata 3661 tattgatatt gataaagttg aaaacataca cttgtgtctg agtacgcgtg gccggcctag 3721 gagctcgaat tctcgtaaaa ctcttcatgg cagtagttat tattctctct ctctctcttt 3781 ttcttttttc ttgagacagg atatttctct gttgcccagg ctggagtgca gtggcacagt 3841 cttggctcac tgcagcctgg acctcctggg ctcaagccat cctcccacct cagcctccca 3901 agtagctggg gctacaggca catggccacc aggccagata atttttcatt tttgtagaga 3961 ctgagtctca ccatgttacc caggtttatt attctcattt tttagatgaa gagactgagg 4021 tccagagaag ctcaatgact tgcctagttt tacaaatctc ctgccatcac atacccctca 4081 gcgtccttaa taagagggag gccaccaact atgtgctggg cactgtggtg gatgctggag 4141 ctatagggtt gagtatataa gaaatggtgt tgctggagca actgttgctt gcttacctga 4201 cctatctgag aattaattag caggggaaca tatttttgtt ttcagattca atataagaac 4261 ttgtgtgggc aaaaataaag atcagtagta ataacagtag ttcccatttg ctgactgtac 4321 tgtcctaagt gcatatatat atacatacac acacgcatac ctatactcct ctaatactca 4381 aaatgatcct gtttatgtat tgttaatatg ctcattttat ttttaaattt ttatttattt 4441 ttatttttat ttatttttga gacggagtct cattctgtcg cggaggctga agtgcagtgg 4501 tgcgatctca gctcagtgcg acctccgcct cccgggttca agtgattctc ctgcctcagc 4561 tccggactag ctgggattac aggcgcccgc ctccacgccc agctaatttt tgtattttta 4621 gtagagatgg ggtttcgcca tgttggccag gctggtctcg tactcctgac cttgagtgat 4681 ccacctgcct cggcctccca aagtgctggg attacaggca tgagccaccg cgccgggcta 4741 atatgctcat tttagtgagg caaaaataga ggctcagagt ctgatttgta caaaactaca 4801 gagcagttaa gtgtcctctc agatgtgtac cctgatctgg gtgactctag gactctaggt 4861 ctcaactgtt acaaccagtt aagggtttgg ggaagcactg ggccaagagt caggaaaatg 4921 gaagccacag gtagtgcaag gtcttgggaa tgggacgtct ggtccaagga ttcacgcgat 4981 gactggaacc cgaagagccg gggcccggtt tacggccgcc atgaagcaac gcgcgccggt 5041 aggtttggga atcagggagc cctctgaata ggagactgag ttgggaggga aaggggcttc 5101 gctgggggag cctcggcttc ttctgggaga aaattcccac ggctacctag tgagcctgca 5161 aactggtagg cgccggcgta ggcgcgcggg cggggccggg ggcggggcct gcggggcgtg 5221 gcggggcggg cagagggcgg ggcctgcttc tcctcagctt caggcggctg cgacgagccc 5281 tcaggcgaac ctctcggctt tcccgcgcgg cgccgcctct tgctgcgcct ccgcctcctc 5341 ctctgctccg ccaccggctt cctcctcctg agcagtcagc ccgcgcgccg gccggctccg 5401 ttatggcgac ccgcagccct ggcgtcgtgg tgagcagctc ggcctgccgg ccctggccgg 5461 ttcaggccca cgcggcaggt ggcggccggg ccctgaggcg cgggatcgat ccatgacacc 5521 cccccgcccc ccatatctca ctataattca ctgtcaaatg gcttttatga tttggagttt 5581 ggggatactt ttaaagacag ggtcttggta gctcctagat ggcttgggat tcacttagta 5641 acccttgctg gcctaaaatt caggaacttc tacctcatct tcccaagtac tgggttagta 5701 gggatgtcca gcatatttag cttgctttaa tgattttgct gtactttttt ctgtgtgtac 5761 aggtgcatgt atatttataa aactaacata atatatttta cccccaaagt accataaccc 5821 taattaatgc tattaaatgt tttgtatgct gggctctggg catactaata taaccaatat 5881 tatacaatgc aaaacaattt tggaatatct tctgtaaaat tatcttatta gtaagttgta 5941 accatctcag acttctaagt ttgagtttgt aaaggatcag ttttttttgg tgacctacac 6001 acacacacac acacacacac acacacacac acacaggggg gtggattctg agaatttaga 6061 gaatttagat ggttaatacc agtttttatt tttattctgt gtgatattta tttattaaga 6121 tttatttatt tattacatgt aggtacactg tatctgtctt cagacacacc agaagagggc 6181 atcagatctc gttacagatg gttgtgagtc accatgtggt tgctgggatt tgaactcagg 6241 acctctggaa gaacagtcag tgctcttaac cgttgagcca tctcaccagc cccgtgatat 6301 tttatttaac ttactagaat atactgtgtt aaaacatatt ttaggggatt ggggagatgg 6361 cccattctgt aaagtgtctg ccatgtcatc ttcaggacct gcgtttgtaa ccccagcatg 6421 gagaagacag agacagggag acccctgggc ttcctggcaa gctagactag caaatttggt 6481 taactaggtt caatgagaga ctcagtctca aaacataaga agtgaatttt ttagacccaa 6541 tttcaatctc tgaactccat acgtatgcac atgtacctgc atataaatat atatacacac 6601 ccataaacac atgcacacac aaaaaatggt tcaaaagttc ttaaaatgta taagagtaga 6661 aagtgtagag ccgggagtgg tggcgcacgc ctttaatccc agcacttggg agcagaggca 6721 ggtggatttc tgagttcgag gccagcctgg tctacagagt gagttccagg acagccagag 6781 ctatacagag aaatcctgcc tcaaaaaacc aaaaataaag tgtatatact aatctttcat 6841 gcctaaaata tttatttttg ccacattttt catacctctt taatattttt ctttttctct 6901 ttgtgcttca aaggaaattt tgtgtcatgt tttacttttc attcttgact ctattcagca 6961 gtaagacgca gcatggcttt cttagagaag tataatctat tacaaaacgg taacatttaa 7021 ctgctctaca taccctcttg tagctaaatt cctctgttaa actaagactt attatctaaa 7081 cgtaattaaa tgttgatatc ttataatgct ccactttgaa acagctgtct ttcttttgca 7141 gattagcgat gatgaaccag gttatgacct agatttgttt tgtataccta atcattatgc 7201 cgaggatttg gaaaaagtgt ttattcctca tggactgatt atggacaggt tagtaagacc 7261 tcgattgaag ttatttttag agggtgtgtg tgtggtgtgt gtgtgtgtgt gtgtgtgtgt 7321 gtgtgtgtgt gtgtaagaga gagaggagag gcatgtgtga tatatatgtg aagtgtattg 7381 catgtacatg gaatccagag aaggacatta ggtgtcctac tctgttgctc tcttattccc 7441 ttgaatcagt gtctcttgct gaacctgggg catacatact gatttggcta ggctgcctgg 7501 ctagcaagac ccagggatct gcttgtctct gccccccagt gctgacgtta caagcaggtg 7561 caaccacatc tggtttttat atgggtactg ggggatctga acttagggcc tgatgcgtgc 7621 ccaagtgctc tcacccgcca agccacctct ccagccaaag ggtttttttt cttgtattaa 7681 ttctaactta gcaactgttg agtagttgat gtttgaatat aagctaagcc aacttattag 7741 aataaatgtt ttctattttt tacttgtatg tgtgagtgcc tgcatgtatg tatgtgaagc 7801 atatgtattc caggatgtca gaattgtgat ggattccctg gaactggagt tacagaccat 7861 tgtgaaccac tgtgtatggg tgctgggaac tgaactttag gtcctctgca aaaacagtaa 7921 ggatcttaac tgataaacta tctttctagc cctaaggctt tctactttta aagttcgttt 7981 ctctttctct ctctctctct ctctctctct ctctctctcc ctctccctcc ctcctcctcc 8041 tcctcctctt tcccccctct cgcctcctct ttctctctcc cctccctctc tctctctcac 8101 acacacacac agacagagag aggagagaga aaggaagcct tactaattaa ttacctagtt 8161 ttagacataa ctagtgggaa tccatagtaa gtaaatcaac attcttgaag agaggttaga 8221 agaattggct ctcgttgtcc atcgaggctg tataagatta ttcttctgtt ttctcagctt 8281 accatacata ttttctacgt agacaatacc aattcaaata cactgtacgt aaagtatatg 8341 cgcacacaca cacacacaca cacacacaca cacacacaag ttacatattg tcatatagta 8401 ctaaaattta tggacttttt catattaata catctagagc tatcatgttt tttatctttt 8461 aaaaattact ttacggggct acagagatgt ctcagcagtt aggagcactg actgctcttc 8521 cagaggtcct gagttcaatt cccagcaacc acatggtggc tcacaaccat ctgtaatggg 8581 atctgatcca ctcttatggt gtgtctgaag acagtgacag tgtactcata tacatgaaat 8641 aaatctttaa aaaattactt taccatcagg gtatatatat atatatatat gtgtgtgtgt 8701 gtgtgtgtgt gtatgaagta tttgctattt gagagggtct cactgtttag ctcagtctgg 8761 cttggaacgt tctgtatagc ccagactggc ctcaactcag cagtccccct gaaaggctct 8821 catatgtgga gattacaggt atgtgctact gtgcccagcc ctacatgtta ctttgttttt 8881 gaaatagggt atcaaatagc ttagactggc ttctaagtca gtgtgtagct aaggatggtc 8941 tgagcttctg aacctgctac ttcaactcct ggtgtgccat catggccagt ttgcacagtt 9001 gtgagagtag aactcaggac ctcctatgtg ttactagaca tgttctctac cagctgagct 9061 acatcccaat tcgcctacat ttacttttat tttttaaaag atttatttat gtatacagca 9121 ctctgcctgc atgtacatct gcatgccaga agagggcacc agatctcatc atacatagtt 9181 gtgagccacc atgtggttgc tggggattga actcagggcc tctggaagag cagtcagtgc 9241 tcttaacctc tgagccatct ctgtagcccc ctacatttat tttaaagatt ataggctatt 9301 ctattcatag atgtgccata actgatttaa ccagatcttc attattttgt acttagtaga 9361 gtatattaga gggaatgaat cttaaataca cattttgaca cttgctcagc tttagacaag 9421 taaaaatcac tggtcaagga aatggtgctt agtggatgct tctgaactgt caaattgtcc 9481 tccaacaagt tacatcattt tatacttcca acgttatgtg agagttcatt tttctttagg 9541 ctcttctagt attttatatt tgagaaataa aatttaacca aaatttgcca atctagtaag 9601 cttgttttta tttgtatctt taaattttat gatagatgtt atagtgtact ctcctctccc 9661 ctcccctccc ctctcctcct cagcaggatc ttgttatgta gccctaaggg atctaatact 9721 tactctgtag tccaggctgt ccttcagctc atgagacaca ccatcatgct tggcatgttt 9781 cttgttctga aaggtatctg gttttaattt tggttgagct ggtgtgttgg tgtatatctg 9841 taatctcagc acaggcaagt agagacaagg attatgatta cagtccagcc tgggctattt 9901 agagggattc tctttaatct ttcctcatgc cccaaaatct tacctttggt atatgaaaaa 9961 tagtctccac ttctgcaaaa tattgcttta tgaagtaaga attaattccc ttcatagaga 10021 caaggaatgt gtcctgtaaa agtttaatgt gtaagaagta tttgttataa aagataaata 10081 ttcagaatct tctttttaat tcctgatttt atttctatag gactgaaaga cttgctcgag 10141 atgtcatgaa ggagatggga ggccatcaca ttgtggccct ctgtgtgctc aaggggggct 10201 ataagttctt tgctgacctg ctggattaca ttaaagcact gaatagaaat agtgatagat 10261 ccattcctat gactgtagat tttatcagac tgaagagcta ctgtgtaagt ataattaact 10321 tataattaaa aaaatagggc cattctagtt ttatctatat tttttttaaa cttgtgcaaa 10381 ctatgctaca taatcaactt acttgattat gtaatatata tagtaatagt tggaattact 10441 attattacat atttattttt ctgtgctggg gtagaaccca gggaactaag tactgtaata 10501 accgagcttc attcatccta gtcattttta attttttttt ttttttttgt tttttcgaga 10561 caaggtttct ctgtgtagcc ctggctgtcc tggaactcac tctgtagacc aggctggcct 10621 cgaactcaga aatctgcctg cctctgcctc ccagtgctga gattaatgac gtgcaccacc 10681 actgcctggc atttttttaa agttttaatt tgagaccctt tctcactaac ttgctcacac 10741 tatcctcgaa ctcattacgt gggctggtct agaaatttca gtcaacttgc ctcagcttct 10801 caagtagctg ggattacagg caggtgctac cacttctggc catgatcatt gttgttgttg 10861 ctattattat tattattatt tcactgatac ttcatatcac aacacagaat agtaactgta 10921 gttcatgacc aacaaaaaag ttgtacagtt tctctgttgt cttattttga tctggatttt 10981 gatttgtttc atggcactgg gcaggctaat ctcaaactgc atggctcaaa tagtccttct 11041 gtcttaatct cccatgttct ggaagtatag gtatcactac acctagctgt attttcatat 11101 tttaaaaaaa acgcctaaga attgctattg aatgaggcgg agagatggct cagcagtcac 11161 aagtacttgt agaggactgg ggtttggtta ctagcacctc aaagatggct tacaattatc 11221 tgtagctcca attccagagg atctgtgtgg taaagctgca tgtatatggg catgcaggta 11281 aaactgctaa acatataaaa atagctcagt cttaagagaa ttggtcgacg taagctcatt 11341 cgagtcgact ttttcgccct ttgacgttgg agtccacgtt ctttaatagt gdgactcttg 11401 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 11461 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 11521 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa 11581 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 11641 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 11701 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 11761 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 11821 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 11881 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 11941 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 12001 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 12061 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 12121 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 12181 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 12241 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 12301 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 12361 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 12421 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 12481 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 12541 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 12601 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 12661 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 12721 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca ggtcgagtag 12781 ggataacagg gtaatggatc ctcgttgggc acctgcactc atgtacacag gcccacatat 12841 agatgtacac acgcatatca ttcaaaatag aaattattta tacaagtagt gcgttggaga 12901 gctggctcag gagttgttaa tgcttgctac ccttgcaaag gacccaagtt caatttccag 12961 cacctgtgtt gggcatctca caaccacatg taaatctagt ttcaggggat ttcacccctc 13021 ttctgaactc ttcaggcact tatacccaca aatggacatc tccacataga gacacatact 13081 tttaaaaaag taatctaggg tctagagaaa tggttataga ccacggactg ggatcagttc 13141 ccagaagcca catcctgtgg ctcacaattg cctgtgaatc aagttctaga tctggtggcc 13201 tcgtctagcc tgcagtcatc aacaaacaca tgcacacatc catacacaca taatttaaaa 13261 gaaagcgaaa gtaacctata atgctaaaca aagcacattc tcagtgtaga ctgcagtggt 13321 gatgaacctt gaaaatatat taatagaagc cagacacgta agtcacaaat ggtataactc 13381 aatttatatg aaattcccag agagtccaaa gttaaaaaaa acaaaaaggc agattattgg 13441 ttgtcagtag ctacagagat gggacattga agatattaat aggtataggg ttgaggattc 13501 atggcctttc cctcttccat gttagcatgt ctattgttgt taaccttatt caactcatgt 13561 tagggcaatc atattgatga gacttaatga gtatagcttg acattcctag aagacagagt 13621 ctcacatcag actctctgat cctctgactc ttagaatctt tcaacaccct cttctgaaat 13681 gatctctgag cctttggttg tgactgaatt atgagaaaga attttatgtt gaatgaattt 13741 tacttcagca tttttacaaa actgaagctg gggatggcgt ttcatgcgtg cagtccctac 13801 catttgccta taagcctgga ggaggcagaa gtagaattag gcttttcagg gacatcttca 13861 gctgcatcat gagcttgagg acaacttgag ctacataaga ttcccatccc cacccccaca 13921 cacacacaca catctagtat gtacctagta tgcagtcttt tcatatgttt aaaatgtaga 13981 aaggtatatg tgaagtctcc cttttcccca caacttagtc cctccaaagt attgcatcag 14041 tttccttgta catgatttta tgcatatata agatacacta tttctctctc tctctctctc 14101 cttccctcct tccctccaac ctctgggtat gagttgaggg ttatgtatgt ttcaagttgt 14161 aataagaatt gccactcaaa catttcatcc cattttaagt taagtttgtc tgattttcca 14221 ctttagaagg gcagccatga tggctcatgc ctataatccc atcctagcac ttgtaagcct 14281 aatacataag actggaggat tgtcatcagc ttgaggctag cctggtctac ctaatgagtt 14341 ccagccagcc aagagtagaa tataagaccc tgtctccaaa aaatgtacca aaggggctgg 14401 aggggtaact cagtggttaa gaacagtagc tgctcttcca gaggacctgg gttcaattcc 14461 cagcacccac atggcagctt gcaactatct gtaactgtcc agctttggga tatccgacac 14521 cattatacca attacataaa atgaaaaaat tgtaaaatgt gctaaaatga gtggggaggg 14581 ggagcatggt gtggttctgt gggcttggga ttttgccatg aatccatgac tatatctcag 14641 caatatgtgg attatttctg gaaacctgtg gggcatgggt gtatgggaag aggggagaga 14701 gagagagccc gcatccagcc agagttcctg tgctctgggc aggcggacgt gggaggactg 14761 ctggacgatt tccacttggc cctgggtggg catctggctg tgtgaagcca ctgaccccac 14821 tcagcggggg gtagataagg ggcatttccc ataccaggtt ctcagactcc agcattccac 14881 gctggataca gcagaagagc tgagaacaga ataggggccc cagggagaca ctctcgtccc 14941 agagataaag aaggcagagg gagagagatt cctacgcagg cgagaggcct tagtctgggc 15001 cttgatgaac agagacggtc tatggtttta gagctttatt gtagaaaggc agggggaaag 15061 agagaaggta gaaagagaga gaccagccat ggccaagagg agagaagggg ggaaaggaga 15121 gagagaagaa aggctagaga gtaagaaagg ttaaagagac agaagtaaaa gcttagagta 15181 agagagagag agagagcaag agggagagta agaaaagtaa aagcttaaga gagcgaggag 15241 gggccagccc ctctaatagt gggcttgtta tcttgttgtt gctaggtaac tgtgaagcgt 15301 ctagcctgaa ggccagaagc ttgggacatt gtctatgtga ctgaaagcca tgagcctcta 15361 ctgtgggggc tgtggaggtg gtaacttgga caggagccag gggttccagg agacatgagg 15421 gaatgccttc tgtcccatgt aggtaggaat taccaccacc ggctttcacc gtagttcact 15481 ggagactgtt tgtacatagc ccattgcccc acagaaacca ggcactcact gtttcatttg 15541 cataagaatt agaaggcttt attttggctg ggattggtga cttgccccag aaattccagc 15601 ttttgggaag ccgaatcctg gagaaccagg agttcaaggt catcctcagg tacctcattt 15661 gttcaagacc agcctgtgct accatgagac cttgtcccta ctcccctcaa ccccaaaaca 15721 cgctgttttc attttatagg ttgtactaac catggaggat ggtcatttgc ctttctcttg 15781 atttcagaag cgtagcgcgg gctttgcacc atgatctagt gtttccagat aggcttgcct 15841 ctccacagcc aggcttatcc tgcttcagtc ccatgtttgg caagggcaac atatgactga 15901 agaacttgca tagccttatg gtgtatcatg actcttagcc ccagtttata actgtctaac 15961 agctggtggc ttttgtgaaa aaggaaagca tcatttgaac ttttaacttt tcagctgttt 16021 gttttgactt tgcataaata ctgagactag ctaggaaatg agcttttgtt tcatttgcta 16081 aaaatgattt ttttttcatg aggcaatttc aagcaatttg aatttagcaa tctctacttg 16141 tctgaaaaga agttattaat taaaaaaaaa caaacaaaca ggaacctatt atgctggcta 16201 gtcacatgaa agaatccaaa tgaacacagt caaggctgaa tcaacacatg aaattatttt 16261 ctaattcccc aaattacatt tatgctaatt agtatgtagt aaaacatttt aattgtttcc 16321 aatgttaaac attacagaaa taattttgat gttttcctaa taacccagcc tttgatcatg 16381 tttgcattta tttgattgtt gtcagtttat taaataatta agtaagagca acaagataaa 16441 atgacttcat gagagtcctt ttctgtaact tagaacgtca gtagtcatag gaactgcggt 16501 cgttccctta cctttcagaa gtaggctaaa gagttgaacg caaaggtggt ttttcgaatc 16561 ttcctgatcc ttaagccccc actaggttta tattctatat ttgatatcgg attcgaaccc 16621 ctcgagctta ggatccggaa cccttaatat aactaagtta ttaggtccct cgaagaggtt 16681 cactagtact ggccattgcg gccgcttcga ggctgcctcg cgcgtttcgg tgatgacggt 16741 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 16801 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 16861 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 16921 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 16981 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 17041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 17101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 17161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 17221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 17281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 17341 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 17401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 17461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 17521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 17581 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 17641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 17701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 17761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 17821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 17881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 17941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 18001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 18061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 18121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 18181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 18241 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 18301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 18361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 18421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 18481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 18541 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca 18601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 18661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 18721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 18781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 18841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 18901 cgcgcacatt tccccgaaaa gtgccacctg acgtg //