LOCUS Ple39\(pEMS1156) 20381 bp DNA circular 8-OCT-2010 SOURCE ORGANISM COMMENT The sequence shown is the expected sequence. Sequence verification of actual construct was not available PCR genotyping: Sense primer: oEMS2744 - 5' GACACTCAGTGATGACTCATGGGTG- 3' Tm=57.2 Deg C Antisense primer: oEMS2745 - 5' TCAGGCAACGCAAAGTTGGT- 3 Tm=55.5 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 1071 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|567532546| COMMENT VNTDBDATE|567532805| COMMENT LSOWNER| COMMENT VNTNAME|Ple39 (pEMS1156)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "Ple39 (pEMS1156)" 0 20381 COMMENT (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 1 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 200 COMMENT 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList) COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 20381 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 20 20 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) COMMENT (CObList COMMENT #0=(CFSignal (CObList) "5' arm of Hprt homology" 86 0 0 14244 18055 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "14244..18055") COMMENT #1=(CFSignal (CObList) "AmpR" 22 0 0 19375 20375 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "19375..20375") COMMENT #2=(CFSignal (CObList) "AmpR" 22 0 0 12793 14218 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "12793..14218") COMMENT #3=(CFSignal (CObList) "3' arm of Hprt homology" 86 0 0 6976 12797 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "6976..12797") COMMENT #4=(CFSignal (CObList) "HPRT complementary sequence" 86 0 0 5198 6975 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "5198..6975") COMMENT #5=(CFSignal (CObList) "I-Sce I" 21 0 0 14225 14242 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "14225..14242") COMMENT #6=(CFSignal (CObList) "FRT (F5mutant)" 21 0 0 4080 4113 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4080..4113") COMMENT #7=(CFSignal (CObList) "Kozak consensus" 21 0 0 4114 4124 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4114..4124") COMMENT #8=(CFSignal (CObList) "EGFP (mutated stop TAA -> TTA)" 21 0 0 4121 4840 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "4121..4840") COMMENT #9=(CFSignal (CObList) "Tetracysteine residue" 21 0 0 4841 4876 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4841..4876") COMMENT #10=(CFSignal (CObList) "NLS" 21 0 0 4877 4949 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4877..4949") COMMENT #11=(CFSignal (CObList) "FRT" 21 0 0 4955 4988 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4955..4988") COMMENT #12=(CFSignal (CObList) "SV40 early polyA signal" 21 0 0 5142 5192 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "5142..5192") COMMENT #13=(CFSignal (CObList) "CRH-D" 21 0 0 139 4071 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "139..4071") COMMENT #14=(CFSignal (CObList) "Prom region" 21 0 0 2313 3227 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2313..3227") COMMENT #15=(CFSignal (CObList) "oEMS2745" 27 0 0 3283 3302 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3283..3302") COMMENT #16=(CFSignal (CObList) "oEMS2744" 27 0 0 2232 2256 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2232..2256") COMMENT #17=(CFSignal (CObList) "Region 6" 21 0 0 139 2312 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) "Region 5'UTR" 21 0 0 3228 4071 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) COMMENT (CObList COMMENT #19=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "aagacactcagtgatgactcatggg" 63 2230 25 0 100 100 100 COMMENT 56.0341 48 -168.3 -433.1 -37.3913 -14.6938) COMMENT (CPrimer 0 "ttaaggaatagtccgcgaacacg" 63 3450 23 1 100 100 100 COMMENT 57.1993 47.8261 -183.5 -474.7 -40.1882 -15.3801) 171 2230 3472 COMMENT 1243 80.3381 51.1665 58.1469) COMMENT #20=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "gacactcagtgatgactcatgggtg" 63 2232 25 0 100 100 100 COMMENT 57.155 52 -163.7 -418.5 -37.1442 -14.8162) COMMENT (CPrimer 0 "ttaaggaatagtccgcgaacacg" 63 3450 23 1 100 100 100 COMMENT 57.1993 47.8261 -183.5 -474.7 -40.1882 -15.3801) 171 2232 3472 COMMENT 1241 80.3711 51.249 58.5062) COMMENT #21=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "actcagtgatgactcatgggtgct" 63 2235 24 0 100 100 100 COMMENT 56.4359 50 -164.7 -422.3 -37.0113 -16.126) COMMENT (CPrimer 0 "ttaaggaatagtccgcgaacacg" 63 3450 23 1 100 100 100 COMMENT 57.1993 47.8261 -183.5 -474.7 -40.1882 -15.3801) 171 2235 3472 COMMENT 1238 80.3544 51.2116 58.2789) COMMENT #22=(CPrimers (CStringList) (CStringList) COMMENT (CPrimer 0 "tcagtgatgactcatgggtgctg" 63 2237 23 0 100 100 100 COMMENT 57.2241 52.1739 -156.2 -397.1 -36.0247 -16.126) COMMENT (CPrimer 0 "ttaaggaatagtccgcgaacacg" 63 3450 23 1 100 100 100 COMMENT 57.1993 47.8261 -183.5 -474.7 -40.1882 -15.3801) 171 2237 3472 COMMENT 1236 80.3544 51.2136 58.5078)) (CObList) (CObList) (CObList) COMMENT (CObList) COMMENT (CTextView 0 COMMENT #23=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #24=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Ple39 (pEMS1156)" COMMENT 2) 5 "" 0 4) COMMENT #25=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'Ple39 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"Comments" 2) 1 "" COMMENT 0 0) COMMENT #37=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #38=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #39=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #40=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 139 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #41=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 139 End: 4071" 1) COMMENT #42=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #43=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 139..4071" 1))) "CRH-D") COMMENT #44=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 139 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #45=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 139 End: 2312" 1))) "Region 6") COMMENT 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COMMENT (CParagraph 4841 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4841 End: 4876" 1) COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4841..4876" 1))) COMMENT "Tetracysteine residue") COMMENT #68=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4877 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4877 End: 4949" 1) COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4877..4949" 1))) "NLS") COMMENT #72=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4955 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4955 End: 4988" 1) COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4955..4988" 1))) "FRT") COMMENT #76=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5142 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5142 End: 5192" 1) COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 5142..5192" 1))) COMMENT "SV40 early polyA signal") COMMENT #80=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 14225 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #81=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 14225 End: 14242" 1) COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 14225..14242" 1))) "I-Sce I"))) COMMENT "Misc. 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3471 43633766) COMMENT #292=(AnlzFontBackColorItem 3474 3498 43633766) COMMENT #293=(AnlzFontBackColorItem 2231 2255 43633766))) (CObList) 1953066364 COMMENT (CStringList) 1869112175 1769104494 (CObList))) FEATURES Location/Qualifiers misc_recomb 14244..18055 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker 19375..20375 /vntifkey="22" /label=AmpR misc_marker 12793..14218 /vntifkey="22" /label=AmpR misc_recomb 6976..12797 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 5198..6975 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 14225..14242 /vntifkey="21" /label=I-Sce\I misc_feature 4080..4113 /vntifkey="21" /label=FRT\(F5mutant) misc_feature 4114..4124 /vntifkey="21" /label=Kozak\consensus misc_feature 4121..4840 /vntifkey="21" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 4841..4876 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4877..4949 /vntifkey="21" /label=NLS misc_feature 4955..4988 /vntifkey="21" /label=FRT misc_feature 5142..5192 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..4071 /vntifkey="21" /label=CRH-D misc_feature 2313..3227 /vntifkey="21" /label=Prom\region primer 3283..3302 /vntifkey="27" /label=oEMS2745 /note="55.5" primer 2232..2256 /vntifkey="27" /label=oEMS2744 /note="57.2" misc_feature 139..2312 /vntifkey="21" /label=Region\6 misc_feature 3228..4071 /vntifkey="21" /label=Region\5'UTR BASE COUNT 5518 a 4561 c 4550 g 5752 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggcccc aaggcactgt cctacttcaa aaagcagaat agcactttta 181 tagaataaat ctatcaggtt atataattgt atttctccat tcaaataaaa aacatccctt 241 tattacaagt atcattttct ctggaaagat aaaagtacat tagcacaagg cagaacttta 301 tagctattag gaattacagt acaaagaaaa caagttgaaa aatgaggtga gggtcaaata 361 tgtatctata tttaacttta tataaattgg agaattaaac atcactgctg ctttgcgcgg 421 ttaagtcttt tcctattatc ctggcaatgt tgttgctcag atcactccta aaatgcaaaa 481 agaatagtga ctgtggaaaa tcagatcctt tctttttcag acataggttt cccacaatca 541 cacttcaagg actcattggc tttcacagtt ataagaccat cttgaattga tgtagccttt 601 ctcctaagta ggacacacat agtcaatccc caacaacaaa tatgtaccat gcataagcag 661 tatattagga ttcatggact aaaagaagtg ggaggtaagt tcataaatga actggaggac 721 agacagataa acacacacac gatcaaaaat aactagctct caaaatggaa aagaatgttc 781 tacagatgga taacagaagc cttgaagatg aaaagatctt gttccaaggt ttgcgaaagg 841 tctgaaccca caacctgtaa catctttgaa atccacagct cctgaatgtt ggcctcccaa 901 gtttctgtcc acctctgtag ccctcattgc ttgctctttc ttgaataact gagtttccct 961 tatgaaaagc ccaacttcat ccattgcaac caattttgtc taggacacgc ctgcctactc 1021 taagagggtt ctcctttgca aggtaggtcc atggatttcc actgggctac atttctgtaa 1081 agaccaactc cttcctctga ccaggaactt tatagctatt aggaattaaa gtacaaagaa 1141 aacaagttgg aaaataaggt gaggctcaaa tatgtatcta tatttaactt tatatgaata 1201 ggggcattaa acatcactgc tgcttagggc cgctaagtct ttttctatta tcctgacaat 1261 gctgttgctc agatcactcc taaaatgcaa aaagaatagt gaatgtggaa aatcagatcc 1321 tttctttttc agacataggt ttgccataat cacccttcaa gggtgattat gcaaacagag 1381 ttgaccattt tatgcatggt aagcagaggt ttggcagctt cttgtttgaa ggtgaccttt 1441 cccagggcag gtaatcctca gaagcagtga aaacagacaa gtttccatac cctgggaccc 1501 tctcttcaaa ggcaaacagt gacatttaaa attttctcct tggagtggta gaaaattact 1561 agtagctcta atcatgaact taaatatcaa atggtaaaga aaaaaaagtc tagctggact 1621 cctcataaaa tgcctttggg cttattatat ttcccagcta gtccaatgat atacatgttc 1681 tgtggtcaaa tgagtctggg aaatgctaaa gacttaggga tttacactgc tgaataaaaa 1741 tttaaaaaca ctaacaaatt ctgcagtaaa atatgaattt aggttttctt taacttttcc 1801 cttccctttt tatgacacca gcaacattcc taggaggctt ctttgggaag gaactctttc 1861 ttcagagtta agtgatgttc atgaatataa atacctgttt gggggtgcct ggttgatgaa 1921 tttctttttt ctgagcaaca attgtaagat gattagttcc tgtaaattgt ctttcatgac 1981 agaaagttca cacacatcct ttcactgccc tccatccatt acctggagtc tcaaacagaa 2041 caatcaatgt attatgatgt aaatagaaag ggcaatcgtg tctgtcaaga ccagggcttg 2101 taagagaagc agaaacgtgc tggttttctg atgctctgag tcatcttccc agggtcagtt 2161 acttctcaac ttatggaaaa atagatgcac aggtaacaaa gtccttgcca ttttctcagg 2221 aaaggaggta agacactcag tgatgactca tgggtgctga cagaattttt gattgatggg 2281 gaactttccc caccaaaccg tggacagcga tggccgcacc ccttactaac cccggtttgt 2341 gccccttcac tatgggagta gctcttgtca tcatctaaaa aacttgaact gcattttgag 2401 agatttattg gccttgcttc tgcaggctca taactccttt atgtgcttgc tcttgggagg 2461 aaaaagcaga tagacgttta aaagctggat gttcctgcac acccctctcc tgatgcttca 2521 ttcttttcca ggcagaaaga tggtgggact ctgtctctag caaagggata tttccagata 2581 ctgaggtgtt gtcagagaca cctggtcagg gaggttagga gaaggggcat ccaggtccac 2641 cccctccaac tggctgctgc tttcctggca gggctgcact gggacacctc acttccttcc 2701 cacttcccct tcctcctccc attcgctgtc tctttgcaca cccctaatat ggcctttcat 2761 agtaagaggt caatatgttt tcacacttgg gaaatctcat tcaagaattt ttgtcaatgg 2821 acaagtcata agaagccctt ccattttagg gctcgttgac gtcaccaaga ggcgataaat 2881 atctgttgat ataattggat gtgagattca gtgttgagat agcaaaattc tgcccctcgt 2941 tccttggcag ggccctatga tttatgcagg agcagaggca gcacgcaatc gagctgtcaa 3001 gagagcgtca gcttattagg caaatgctgc gtggtttttg aagagggtcg acactataaa 3061 atcccactcc aggctctgga gtggagaaac tcagagacca agtccattga gagactgagg 3121 ggaaagagag gagagaaaga aaaagagagt gggaacagta aagagaaagg aagacaacct 3181 ccagagaaag cccccggaga cgtctctctg cagagaggcg gcagcacccg gctcacctgc 3241 gaagcgcctg ggaaggtagg gagcgcctgg acggaacacg ccaccaactt tgcgttgcct 3301 gagctgccgc gatgtgcgcc gcggagccgg ctgcccctgc ctgtgtacgt gtgtatgcat 3361 gtgtgtgcgt gcgtgtgtgt gtgtgtatgc atgtgtgtat gcgtatgtgt gtgtgtgtat 3421 gcgcgcgtgc gtgcgtgtgt gtgtgcgcgc gtgttcgcgg actattcctt aagattacaa 3481 tagtagctgg gatgtggcaa aagcaaactt agacggctgc tcttcgttac ccgtgctgcc 3541 agttgctcct tgctggctgt gtaaaggagc tcccccaccc ccttctatcc taacccccga 3601 gataaggcag caagggcaga agtggagagg gaggaccatg aggcagtagt tctcaatttg 3661 gaagaaaatg ctgaaatatc cggaagggag gtagtcagcg cgggtgggat cttaaatctg 3721 tggctcttct ctaggatcgg gaaaattagt tttgaggatc gttaggaaga ggacgcaggg 3781 accgtccttt cagagtctag aaagaagggt cactcctggc cggtgagcgc atgagcttag 3841 gtgcggagcc ctgcaggcgc cccctttccc cgctccccag gcgcttcagc accgcggaca 3901 gcgcccatcc gaatcactga ggccaaagcc cagcacgtct aaggcagtcc cgtaggaaga 3961 ccccgtgtgc acctcccagc tgagcgaact ctaaccaact ctttctttct ctcttcccct 4021 ctctcattcc gccctccgcc cacctctgtg ccgcagcgag tgcccctaac aggcgcgccg 4081 aagttcctat tcttcaaaag gtataggaac ttccgccacc atggtgagca agggcgagga 4141 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 4201 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 4261 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 4321 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 4381 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 4441 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 4501 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 4561 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 4621 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 4681 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 4741 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 4801 cgccgggatc actctcggca tggacgagct gtacaagtta tttctgaact gctgcccggg 4861 ctgctgcatg gaaccggatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa 4921 ggtagatcca aaaaagaaga gaaaggtaat ataagaagtt cctattctct agaaagtata 4981 ggaacttcag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt 5041 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 5101 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 5161 aaatttcaca aataaagcat ttttttcact gcgaattctc gtaaaactct tcatggcagt 5221 agttattatt ctctctctct ctctttttct tttttcttga gacaggatat ttctctgttg 5281 cccaggctgg agtgcagtgg cacagtcttg gctcactgca gcctggacct cctgggctca 5341 agccatcctc ccacctcagc ctcccaagta gctggggcta caggcacatg gccaccaggc 5401 ccagataatt tttcattttt gtagagactg agtctcacca tgttacccag gtttattatt 5461 ctcatttttt agatgaagag actgaggtcc agagaagctc aatgacttgc ctagttttac 5521 aaatctcctg ccatcacata cccctcagcg tccttaataa gagggaggcc accaactatg 5581 tgctgggcac tgtggtggat gctggagcta tagggttgag tatataagaa atggtgttgc 5641 tggagcaact gttgcttgct tacctgacct atctgagaat taattagcag gggaacatat 5701 ttttgttttc agattcaata taagaacttg tgtgggcaaa aataaagatc agtagtaata 5761 acagtagttc ccatttgctg actgtactgt cctaagtgca tatatatata catacacaca 5821 cgcataccta tactcctcta atactcaaaa tgatcctgtt tatgtattgt taatatgctc 5881 attttatttt taaattttta tttattttta tttttattta tttttgagac ggagtctcat 5941 tctgtcgcgg aggctgaagt gcagtggtgc gatctcagct cagtgcgacc tccgcctccc 6001 gggttcaagt gattctcctg cctcagcctc cggactagct gggattacag gcgcccgcct 6061 ccacgcccag ctaatttttg tatttttagt agagatgggg tttcgccatg ttggccaggc 6121 tggtctcgta ctcctgacct tgagtgatcc acctgcctcg gcctcccaaa gtgctgggat 6181 tacaggcatg agccaccgcg ccgggctaat atgctcattt tagtgaggca aaaatagagg 6241 ctcagagtct gatttgtaca aaactacaga gcagttaagt gtcctctcag atgtgtaccc 6301 tgatctgggt gactctagga ctctaggtct caactgttac aaccagttaa gggtttgggg 6361 aagcactggg ccaagagtca ggaaaatgga agccacaggt agtgcaaggt cttgggaatg 6421 ggacgtctgg tccaaggatt cacgcgatga ctggaacccg aagagccggg gcccggttta 6481 cggccgccat gaagcaacgc gcgccggtag gtttgggaat cagggagccc tctgaatagg 6541 agactgagtt gggagggaaa ggggcttcgc tgggggagcc tcggcttctt ctgggagaaa 6601 attcccacgg ctacctagtg agcctgcaaa ctggtaggcg ccggcgtagg cgcgcgggcg 6661 gggccggggg cggggcctgc ggggcgtggc ggggcgggca gagggcgggg cctgcttctc 6721 ctcagcttca ggcggctgcg acgagccctc aggcgaacct ctcggctttc ccgcgcggcg 6781 ccgcctcttg ctgcgcctcc gcctcctcct ctgctccgcc accggcttcc tcctcctgag 6841 cagtcagccc gcgcgccggc cggctccgtt atggcgaccc gcagccctgg cgtcgtggtg 6901 agcagctcgg cctgccggcc ctggccggtt caggcccacg cggcaggtgg cggccgggcc 6961 ctgaggcgcg ggatcgatcc atgacacccc cccgcccccc atatctcact ataattcact 7021 gtcaaatggc ttttatgatt tggagtttgg ggatactttt aaagacaggg tcttggtagc 7081 tcctagatgg cttgggattc acttagtaac ccttgctggc ctaaaattca ggaacttcta 7141 cctcatcttc ccaagtactg ggttagtagg gatgtccagc atatttagct tgctttaatg 7201 attttgctgt acttttttct gtgtgtacag gtgcatgtat atttataaaa ctaacataat 7261 atattttacc cccaaagtac cataacccta attaatgcta ttaaatgttt tgtatgctgg 7321 gctctgggca tactaatata accaatatta tacaatgcaa aacaattttg gaatatcttc 7381 tgtaaaatta tcttattagt aagttgtaac catctcagac ttctaagttt gagtttgtaa 7441 aggatcagtt ttttttggtg acctacacac acacacacac acacacacac acacacacac 7501 acaggggggt ggattctgag aatttagaga atttagatgg ttaataccag tttttatttt 7561 tattctgtgt gatatttatt tattaagatt tatttattta ttacatgtag gtacactgta 7621 tctgtcttca gacacaccag aagagggcat cagatctcgt tacagatggt tgtgagtcac 7681 catgtggttg ctgggatttg aactcaggac ctctggaaga acagtcagtg ctcttaaccg 7741 ttgagccatc tcaccagccc cgtgatattt tatttaactt actagaatat actgtgttaa 7801 aacatatttt aggggattgg ggagatggcc cattctgtaa agtgtctgcc atgtcatctt 7861 caggacctgc gtttgtaacc ccagcatgga gaagacagag acagggagac ccctgggctt 7921 cctggcaagc tagactagca aatttggtta actaggttca atgagagact cagtctcaaa 7981 acataagaag tgaatttttt agacccaatt tcaatctctg aactccatac gtatgcacat 8041 gtacctgcat ataaatatat atacacaccc ataaacacat gcacacacaa aaaatggttc 8101 aaaagttctt aaaatgtata agagtagaaa gtgtagagcc gggagtggtg gcgcacgcct 8161 ttaatcccag cacttgggag cagaggcagg tggatttctg agttcgaggc cagcctggtc 8221 tacagagtga gttccaggac agccagagct atacagagaa atcctgcctc aaaaaaccaa 8281 aaataaagtg tatatactaa tctttcatgc ctaaaatatt tatttttgcc acatttttca 8341 tacctcttta atatttttct ttttctcttt gtgcttcaaa ggaaattttg tgtcatgttt 8401 tacttttcat tcttgactct attcagcagt aagacgcagc atggctttct tagagaagta 8461 taatctatta caaaacggta acatttaact gctctacata ccctcttgta gctaaattcc 8521 tctgttaaac taagacttat tatctaaacg taattaaatg ttgatatctt ataatgctcc 8581 actttgaaac agctgtcttt cttttgcaga ttagcgatga tgaaccaggt tatgacctag 8641 atttgttttg tatacctaat cattatgccg aggatttgga aaaagtgttt attcctcatg 8701 gactgattat ggacaggtta gtaagacctc gattgaagtt atttttagag ggtgtgtgtg 8761 tggtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtaagagaga gaggagaggc 8821 atgtgtgata tatatgtgaa gtgtattgca tgtacatgga atccagagaa ggacattagg 8881 tgtcctactc tgttgctctc ttattccctt gaatcagtgt ctcttgctga acctggggca 8941 tacatactga tttggctagg ctgcctggct agcaagaccc agggatctgc ttgtctctgc 9001 cccccagtgc tgacgttaca agcaggtgca accacatctg gtttttatat gggtactggg 9061 ggatctgaac ttagggcctg atgcgtgccc aagtgctctc acccgccaag ccacctctcc 9121 agccaaaggg ttttttttct tgtattaatt ctaacttagc aactgttgag tagttgatgt 9181 ttgaatataa gctaagccaa cttattagaa taaatgtttt ctatttttta cttgtatgtg 9241 tgagtgcctg catgtatgta tgtgaagcat atgtattcca ggatgtcaga attgtgatgg 9301 attccctgga actggagtta cagaccattg tgaaccactg tgtatgggtg ctgggaactg 9361 aactttaggt cctctgcaaa aacagtaagg atcttaactg ataaactatc tttctagccc 9421 taaggctttc tacttttaaa gttcgtttct ctttctctct ctctctctct ctctctctct 9481 ctctctccct ctccctccct cctcctcctc ctcctctttc ccccctctcg cctcctcttt 9541 ctctctcccc tccctctctc tctctcacac acacacacag acagagagag gagagagaaa 9601 ggaagcctta ctaattaatt acctagtttt agacataact agtgggaatc catagtaagt 9661 aaatcaacat tcttgaagag aggttagaag aattggctct cgttgtccat cgaggctgta 9721 taagattatt cttctgtttt ctcagcttac catacatatt ttctacgtag acaataccaa 9781 ttcaaataca ctgtacgtaa agtatatgcg cacacacaca cacacacaca cacacacaca 9841 cacacaagtt acatattgtc atatagtact aaaatttatg gactttttca tattaataca 9901 tctagagcta tcatgttttt tatcttttaa aaattacttt acggggctac agagatgtct 9961 cagcagttag gagcactgac tgctcttcca gaggtcctga gttcaattcc cagcaaccac 10021 atggtggctc acaaccatct gtaatgggat ctgatccact cttatggtgt gtctgaagac 10081 agtgacagtg tactcatata catgaaataa atctttaaaa aattacttta ccatcagggt 10141 atatatatat atatatatgt gtgtgtgtgt gtgtgtgtgt atgaagtatt tgctatttga 10201 gagggtctca ctgtttagct cagtctggct tggaacgttc tgtatagccc agactggcct 10261 caactcagca gtccccctga aaggctctca tatgtggaga ttacaggtat gtgctactgt 10321 gcccagccct acatgttact ttgtttttga aatagggtat caaatagctt agactggctt 10381 ctaagtcagt gtgtagctaa ggatggtctg agcttctgaa cctgctactt caactcctgg 10441 tgtgccatca tggccagttt gcacagttgt gagagtagaa ctcaggacct cctatgtgtt 10501 actagacatg ttctctacca gctgagctac atcccaattc gcctacattt acttttattt 10561 tttaaaagat ttatttatgt atacagcact ctgcctgcat gtacatctgc atgccagaag 10621 agggcaccag atctcatcat acatagttgt gagccaccat gtggttgctg gggattgaac 10681 tcagggcctc tggaagagca gtcagtgctc ttaacctctg agccatctct gtagccccct 10741 acatttattt taaagattat aggctattct attcatagat gtgccataac tgatttaacc 10801 agatcttcat tattttgtac ttagtagagt atattagagg gaatgaatct taaatacaca 10861 ttttgacact tgctcagctt tagacaagta aaaatcactg gtcaaggaaa tggtgcttag 10921 tggatgcttc tgaactgtca aattgtcctc caacaagtta catcatttta tacttccaac 10981 gttatgtgag agttcatttt tctttaggct cttctagtat tttatatttg agaaataaaa 11041 tttaaccaaa atttgccaat ctagtaagct tgtttttatt tgtatcttta aattttatga 11101 tagatgttat agtgtactct cctctcccct cccctcccct ctcctcctca gcaggatctt 11161 gttatgtagc cctaagggat ctaatactta ctctgtagtc caggctgtcc ttcagctcat 11221 gagacacacc atcatgcttg gcatgtttct tgttctgaaa ggtatctggt tttaattttg 11281 gttgagctgg tgtgttggtg tatatctgta atctcagcac aggcaagtag agacaaggat 11341 tatgattaca gtccagcctg ggctatttag agggattctc tttaatcttt cctcatgccc 11401 caaaatctta cctttggtat atgaaaaata gtctccactt ctgcaaaata ttgctttatg 11461 aagtaagaat taattccctt catagagaca aggaatgtgt cctgtaaaag tttaatgtgt 11521 aagaagtatt tgttataaaa gataaatatt cagaatcttc tttttaattc ctgattttat 11581 ttctatagga ctgaaagact tgctcgagat gtcatgaagg agatgggagg ccatcacatt 11641 gtggccctct gtgtgctcaa ggggggctat aagttctttg ctgacctgct ggattacatt 11701 aaagcactga atagaaatag tgatagatcc attcctatga ctgtagattt tatcagactg 11761 aagagctact gtgtaagtat aattaactta taattaaaaa aatagggcca ttctagtttt 11821 atctatattt tttttaaact tgtgcaaact atgctacata atcaacttac ttgattatgt 11881 aatatatata gtaatagttg gaattactat tattacatat ttatttttct gtgctggggt 11941 agaacccagg gaactaagta ctgtaataac cgagcttcat tcatcctagt catttttaat 12001 tttttttttt ttttttgttt tttcgagaca aggtttctct gtgtagccct ggctgtcctg 12061 gaactcactc tgtagaccag gctggcctcg aactcagaaa tctgcctgcc tctgcctccc 12121 agtgctgaga ttaatgacgt gcaccaccac tgcctggcat ttttttaaag ttttaatttg 12181 agaccctttc tcactaactt gctcacacta tcctcgaact cattacgtgg gctggtctag 12241 aaatttcagt caacttgcct cagcttctca agtagctggg attacaggca ggtgctacca 12301 cttctggcca tgatcattgt tgttgttgct attattatta ttattatttc actgatactt 12361 catatcacaa cacagaatag taactgtagt tcatgaccaa caaaaaagtt gtacagtttc 12421 tctgttgtct tattttgatc tggattttga tttgtttcat ggcactgggc aggctaatct 12481 caaactgcat ggctcaaata gtccttctgt cttaatctcc catgttctgg aagtataggt 12541 atcactacac ctagctgtat tttcatattt taaaaaaaac gcctaagaat tgctattgaa 12601 tgaggcggag agatggctca gcagtcacaa gtacttgtag aggactgggg tttggttact 12661 agcacctcaa agatggctta caattatctg tagctccaat tccagaggat ctgtgtggta 12721 aagctgcatg tatatgggca tgcaggtaaa actgctaaac atataaaaat agctcagtct 12781 taagagaatt ggtcgacttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 12841 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 12901 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 12961 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 13021 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 13081 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 13141 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 13201 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 13261 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 13321 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 13381 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 13441 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 13501 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 13561 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 13621 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 13681 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 13741 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 13801 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 13861 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 13921 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 13981 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 14041 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 14101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 14161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccaggt 14221 cgagtaggga taacagggta atggatcctc gttgggcacc tgcactcatg tacgcaggcc 14281 cacatataga tgtacacacg catatcattc aaaatagaaa ttatttatac aagtagtgcg 14341 ttggagagct ggctcaggag ttgttaatgc ttgctaccct tgcaaaggac ccaagttcaa 14401 tttccagcac ctgtgttggg catctcacaa ccacatgtaa atctagtttc aggggatttc 14461 acccctcttc tgaactcttc aggcacttat acccacaaat ggacatctcc acatagagac 14521 acatactttt aaaaaagtaa tctagggtct agagaaatgg ttatagacca cggactggga 14581 tcagttccca gaagccacat cctgtggctc acaattgcct gtgaatcaag ttctagatct 14641 ggtggcctcg tctagcctgc agtcatcaac aaacacatgc acacatccat acacacataa 14701 tttaaaagaa agcgaaagta acctataatg ctaaacaaag cacattctca gtgtagactg 14761 cagtggtgat gaaccttgaa aatatattaa tagaagccag acacgtaagt cacaaatggt 14821 ataactcaat ttatatgaaa ttcccagaga gtccaaagtt aaaaaaaaca aaaaggcaga 14881 ttattggttg tcagtagcta cagagatggg acattgaaga tattaatagg tatagggttg 14941 aggattcatg gcctttccct cttccatgtt agcatgtcta ttgttgttaa ccttattcaa 15001 ctcatgttag ggcaatcata ttgatgagac ttaatgagta tagcttgaca ttcctagaag 15061 acagagtctc acatcagact ctctgatcct ctgactctta gaatctttca acaccctctt 15121 ctgaaatgat ctctgagcct ttggttgtga ctgaattatg agaaagaatt ttatgttgaa 15181 tgaattttac ttcagcattt ttacaaaact gaagctgggg atggcgtttc atgcgtgcag 15241 tccctaccat ttgcctataa gcctggagga ggcagaagta gaattaggct tttcagggac 15301 atcttcagct gcatcatgag cttgaggaca acttgagcta cataagattc ccatccccac 15361 ccccacacac acacacacat ctagtatgta cctagtatgc agtcttttca tatgtttaaa 15421 atgtagaaag gtatatgtga agtctccctt ttccccacaa cttagtccct ccaaagtatt 15481 gcatcagttt ccttgtacat gattttatgc atatataaga tacactattt ctctctctct 15541 ctctctcctt ccctccttcc ctccaacctc tgggtatgag ttgagggtta tgtatgtttc 15601 aagttgtaat aagaattgcc actcaaacat ttcatcccat tttaagttaa gtttgtctga 15661 ttttccactt tagaagggca gccatgatgg ctcatgccta taatcccatc ctagcacttg 15721 taagcctaat acataagact ggaggattgt catcagcttg aggctagcct ggtctaccta 15781 atgagttcca gccagccaag agtagaatat aagaccctgt ctccaaaaaa tgtaccaaag 15841 gggctggagg ggtaactcag tggttaagaa cagtagctgc tcttccagag gacctgggtt 15901 caattcccag cacccacatg gcagcttgca actatctgta actgtccagc tttgggatat 15961 ccgacaccat tataccaatt acataaaatg aaaaaattgt aaaatgtgct aaaatgagtg 16021 gggaggggga gcatggtgtg gttctgtggg cttgggattt tgccatgaat ccatgactat 16081 atctcagcaa tatgtggatt atttctggaa acctgtgggg catgggtgta tgggaagagg 16141 ggagagagag agagcccgca tccagccaga gttcctgtgc tctgggcagg cggacgtggg 16201 aggactgctg gacgatttcc acttggccct gggtgggcat ctggctgtgt gaagccactg 16261 accccactca gcggggggta gataaggggc atttcccata ccaggttctc agactccagc 16321 attccacgct ggatacagca gaagagctga gaacagaata ggggccccag ggagacactc 16381 tcgtcccaga gataaagaag gcagagggag agagattcct acgcaggcga gaggccttag 16441 tctgggcctt gatgaacaga gacggtctat ggttttagag ctttattgta gaaaggcagg 16501 gggaaagaga gaaggtagaa agagagagac cagccatggc caagaggaga gaagggggga 16561 aaggagagag agaagaaagg ctagagagta agaaaggtta aagagacaga agtaaaagct 16621 tagagtaaga gagagagaga gagcaagagg gagagtaaga aaagtaaaag cttaagagag 16681 cgaggagggg ccagcccctc taatagtggg cttgttatct tgttgttgct aggtaactgt 16741 gaagcgtcta gcctgaaggc cagaagcttg ggacattgtc tatgtgactg aaagccatga 16801 gcctctactg tgggggctgt ggaggtggta acttggacag gagccagggg ttccaggaga 16861 catgagggaa tgccttctgt cccatgtagg taggaattac caccaccggc tttcaccgta 16921 gttcactgga gactgtttgt acatagccca ttgccccaca gaaaccaggc actcactgtt 16981 tcatttgcat aagaattaga aggctttatt ttggctggga ttggtgactt gccccagaaa 17041 ttccagcttt tgggaagccg aatcctggag aaccaggagt tcaaggtcat cctcaggtac 17101 ctcatttgtt caagaccagc ctgtgctacc atgagacctt gtccctactc ccctcaaccc 17161 caaaacacgc tgttttcatt ttataggttg tactaaccat ggaggatggt catttgcctt 17221 tctcttgatt tcagaagcgt agcgcgggct ttgcaccatg atctagtgtt tccagatagg 17281 cttgcctctc cacagccagg cttatcctgc ttcagtccca tgtttggcaa gggcaacata 17341 tgactgaaga acttgcatag ccttatggtg tatcatgact cttagcccca gtttataact 17401 gtctaacagc tggtggcttt tgtgaaaaag gaaagcatca tttgaacttt taacttttca 17461 gctgtttgtt ttgactttgc ataaatactg agactagcta ggaaatgagc ttttgtttca 17521 tttgctaaaa atgatttttt tttcatgagg caatttcaag caatttgaat ttagcaatct 17581 ctacttgtct gaaaagaagt tattaattaa aaaaaaacaa acaaacagga acctattatg 17641 ctggctagtc acatgaaaga atccaaatga acacagtcaa ggctgaatca acacatgaaa 17701 ttattttcta attccccaaa ttacatttat gctaattagt atgtagtaaa acattttaat 17761 tgtttccaat gttaaacatt acagaaataa ttttgatgtt ttcctaataa cccagccttt 17821 gatcatgttt gcatttattt gattgttgtc agtttattaa ataattaagt aagagcaaca 17881 agataaaatg acttcatgag agtccttttc tgtaacttag aacgtcagta gtcataggaa 17941 ctgcggtcgt tcccttacct ttcagaagta ggctaaagag ttgaacgcaa aggtggtttt 18001 tcgaatcttc ctgatcctta agcccccact aggtttatat tctatatttg atatcggatt 18061 cgaacccctc gagcttagga tccggaaccc ttaatataac taagttatta ggtccctcga 18121 agaggttcac tagtactggc cattgcggcc gcttcgaggc tgcctcgcgc gtttcggtga 18181 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 18241 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 18301 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca 18361 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 18421 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 18481 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 18541 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 18601 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 18661 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 18721 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 18781 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 18841 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 18901 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 18961 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 19021 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 19081 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 19141 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 19201 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 19261 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 19321 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 19381 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 19441 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 19501 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 19561 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 19621 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 19681 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 19741 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 19801 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 19861 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 19921 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 19981 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 20041 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 20101 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 20161 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 20221 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 20281 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 20341 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg g //