LOCUS Ple176\(pEMS1364 19679 bp DNA circular 3-FEB-2010 SOURCE ORGANISM COMMENT PCR genotyping: Antisense primer: oEMS2367 - 5' GTGGAAGGAAAAGCGGCAAG - 3' Tm=56.2 Deg C Sense primer: oEMS2406 - 5' CATGAGCAAAAGAAGATGGGACAG - 3 Tm=56.7 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 548 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|543237970| COMMENT VNTDBDATE|544983618| COMMENT LSOWNER| COMMENT VNTNAME|Ple176 (pEMS1364)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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50266367))) (CObList) 34078928 COMMENT (CStringList) 10 327734 (CObList))) FEATURES Location/Qualifiers misc_recomb 13542..17353 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(18452..19673) /vntifkey="22" /label=AmpR misc_marker 12091..13516 /vntifkey="22" /label=AmpR misc_recomb 8450..12095 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4496..8449 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 13523..13540 /vntifkey="21" /label=I-Sce\I misc_feature 3378..3411 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 3412..3422 /vntifkey="21" /label=Kozak\consensus misc_feature 3419..4138 /vntifkey="21" /label=EGFP misc_feature 4139..4174 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4175..4247 /vntifkey="21" /label=NLS misc_feature 4253..4286 /vntifkey="21" /label=MFRT misc_feature 4440..4490 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..3369 /vntifkey="21" /label=Ple176\(RGS16-B) /note="1306" primer 2508..2531 /vntifkey="27" /label=oEMS2406 primer 3036..3055 /vntifkey="27" /label=oEMS2367 misc_feature 2789..3369 /vntifkey="21" /label=Prom\Region misc_feature 139..1743 /vntifkey="21" /label=Region\1 misc_feature 1744..2788 /vntifkey="21" /label=Region\2 BASE COUNT 5152 a 4513 c 4588 g 5426 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccgg aagtgtgcac accagttgtt agtgactatc attgtgttta 181 ctgcagccag ggtgtttggg acgcctgggg acttggatcc agaaagttcc cctgtggtca 241 catttggagc agcactacag ctagatggct ggcggaggag gtgtggctca cccaggctca 301 gtcacacagc tcaccgcgtg tgcccccctg ctcaaatgtg tggggcctat tagggagagc 361 tcatttctat ctctgcaaaa acatcatgca ccccctgctg tgctcttctc tctgtccacc 421 tgggcctgtt tctaggacag ctgctgtgtc aggggctgag ggcctgggag cttgtgaaag 481 gaccacctgt tcccattgct ttctccggcc ctcctccccc gccgccactc tgccggccca 541 gcctcccagc tgctcacaca tcacaccacg tttctccctc tgctcaggtg ttgtgagcac 601 attgtgagag gcatgatccc ttccaacttg tcgccacatt acagctagtg ttggtgcttt 661 tgttgagcct cccctgcttt cctataaaat ggtttttctt ctgtgcccct gcaccagact 721 gccccacccc caccccctac tctccctgtt gacatgggga ctcctggaag gtgactcaaa 781 ggaggaggag gtaccccaca gaaagtaggt ccttaactcc caggagatga caaagcccct 841 cccatgcctc tgacctgaca tctctgcacg tgtgtgtggt attcacatgt tggggcttgc 901 tgttcctggg gtccttatgt ctcatctgtg gcctaggtaa gcacctagaa atttctgact 961 agaacaagga ggagaaatgg cctgaagtag cagccaaggt ccagggggag agatgctcag 1021 aggctaggga acatggggac aaggcattga atagccctgg ggttggacct tgtggataga 1081 actgggtttc tagcctggca ctcccattca ctaggtgtgt cactgtgggg agattactta 1141 cgcaccgatg tctgtttccc cttctgtaca ggtggcatct gtaaggccct gagtccagcg 1201 ttggatcatg gagggcccta aaaatggtag tttccttttc tctaagacag ggcctggagg 1261 aagaccagac atcaacctcc caagctggct gaaatcgtct tactgtctga cacagagccg 1321 ggttctccct agtacatgct gctctggaat acctaggaga ggaagaggaa ggaggcaagg 1381 taagctgggg gcttttcaca ctaaaggcct acagaggggt aggtggagtc ggcataggtg 1441 ccatgtgtgt ttggtgtctt gcttccgact ctagctgggc tctggagcca ctgatgtctg 1501 cttgaccctt ccctgatttt gctccagttt gctctgcact gggagactgc aagccacagg 1561 gggaggggag tgtaagaggg ctgcagagta ataagtttca ctgcataatt cagccctcct 1621 gggacccgtt tccagaaagc tgactggctg aagtttagct gagaatgcct gcaatctgga 1681 ggatctgggg caggcagggt tgacagttaa tctccaagga tattcaccat gtttggagtg 1741 ggaaaaggac taggggacgg gaaatataaa catcaccttt cagtcatttg caaatcccag 1801 gcctcacgtt tcccccctct aatctttcat tctgactggc ttctcagggt cttggaaaat 1861 cattacagta aaatgcagat tctaataagc cttgtttcta agtcttagtg aatggaaatg 1921 agatactctc agaaaaatga cttttgtgac aaaggtgaag aaggcaagga ggaggtatgg 1981 tgggaaagag gaagacattt agaaactcag gcttcttagg taactctctt acttcggaaa 2041 tggaagtgtt aaggtgagat gggcccattt attactgatg acttacttgg tactttaggg 2101 aatgcagcaa ggtgatggca ggggtgggca ggggtttgca gagtggagac agcatgggct 2161 ggtgagcaca gggagaggtg gaggaagctg ggcgctggca ggcagggaaa gttcgggagc 2221 ctggagggag gtagactatc gggttatagg gtggggtgaa gtggacctta cagtcaggca 2281 ggtaagctca cgggtttcag accacaaaac gctaagatct gggcctggga agggccaagc 2341 cagcatgtga gaaccaagaa agcataaacc tcagagggcc aggcgccatc ccccagctct 2401 caggttccca ggaggatggg gaggaacaaa ggtcttgggt tttgacacat cccaaagact 2461 ttgggagctg cccctggtct ccctcccagg tgggaaccag gcttttccat gagcaaaaga 2521 agatgggaca gcatgggctg gaaagtgtgg caggggaaca cagagctgaa ttctgagttt 2581 tcctgaaaaa gagatataaa aacccacatg ctaacagagg gacttgagca gagaaaggga 2641 ctctccacag ccatactcgg gaactgtata cacaagggac tgggaagtga agcagcagaa 2701 aaatccagag cctgtgatga catcacagct aagggcaaag acaggaagtg gaaaatgtga 2761 atggggaggg aggaatggat tgagcaaggg gtacttatca gtataatcag atgctgtttt 2821 gcccaggttt gcaccctgtg cgcctgtcct caggacccag caggctcaga cttgcctgcg 2881 atcaaagaaa gccctgtgtg cctcccagac ccaccacccc cgtgcaaact ctctcaacac 2941 gacaccgctg cgtgactttc ctgctgttac tagaggtcgg caggtacttt tccctaggaa 3001 actgggtgtg cacgtctcat ccccgtgcca attctcttgc cgcttttcct tccacacgcg 3061 gcgtcctctg ggcggagggt gcagctccgc gtgctccagc tgccgcgaaa gttgcacccg 3121 gcccagtctg gctgcgggcc ccgcctccgc cttctcagcg cccagcccca ccctccgatt 3181 ggtcagcagt gcccagcccc gccccgctcc tccgagggga tttaaagcag cggccgctca 3241 gtctgggcgc ttgcaggctg ctaaacccaa ccgcagttga ctagcacctg ctaccgcgcc 3301 tttgcttcct ggcgcacgcg gagcctcctg gagcctgcca ccatcctgcc tactacgtgc 3361 tgccctgcgg gcgcgccgaa gttcctattc ttcaaaaggt ataggaactt ccgccaccat 3421 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 3481 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 3541 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 3601 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 3661 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 3721 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 3781 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 3841 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 3901 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 3961 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 4021 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 4081 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagttatt 4141 tctgaactgc tgcccgggct gctgcatgga accggatcca aaaaagaaga gaaaggtaga 4201 tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtaatat aagaagttcc 4261 tattctctag aaagtatagg aacttcagcg gccgcgactc tagatcataa tcagccatac 4321 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 4381 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa 4441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc gaattctcgt 4501 aaaactcttc atggcagtag ttattattct ctctctctct ctttttcttt tttcttgaga 4561 caggatattt ctctgttgcc caggctggag tgcagtggca cagtcttggc tcactgcagc 4621 ctggacctcc tgggctcaag ccatcctccc acctcagcct cccaagtagc tggggctaca 4681 ggcacatggc caccaggccc agataatttt tcatttttgt agagactgag tctcaccatg 4741 ttacccaggt ttattattct cattttttag atgaagagac tgaggtccag agaagctcaa 4801 tgacttgcct agttttacaa atctcctgcc atcacatacc cctcagcgtc cttaataaga 4861 gggaggccac caactatgtg ctgggcactg tggtggatgc tggagctata gggttgagta 4921 tataagaaat ggtgttgctg gagcaactgt tgcttgctta cctgacctat ctgagaatta 4981 attagcaggg gaacatattt ttgttttcag attcaatata agaacttgtg tgggcaaaaa 5041 taaagatcag tagtaataac agtagttccc atttgctgac tgtactgtcc taagtgcata 5101 tatatataca tacacacacg catacctata ctcctctaat actcaaaatg atcctgttta 5161 tgtattgtta atatgctcat tttattttta aatttttatt tatttttatt tttatttatt 5221 tttgagacgg agtctcattc tgtcgcggag gctgaagtgc agtggtgcga tctcagctca 5281 gtgcgacctc cgcctcccgg gttcaagtga ttctcctgcc tcagcctccg gactagctgg 5341 gattacaggc gcccgcctcc acgcccagct aatttttgta tttttagtag agatggggtt 5401 tcgccatgtt ggccaggctg gtctcgtact cctgaccttg agtgatccac ctgcctcggc 5461 ctcccaaagt gctgggatta caggcatgag ccaccgcgcc gggctaatat gctcatttta 5521 gtgaggcaaa aatagaggct cagagtctga tttgtacaaa actacagagc agttaagtgt 5581 cctctcagat gtgtaccctg atctgggtga ctctaggact ctaggtctca actgttacaa 5641 ccagttaagg gtttggggaa gcactgggcc aagagtcagg aaaatggaag ccacaggtag 5701 tgcaaggtct tgggaatggg acgtctggtc caaggattca cgcgatgact ggaacccgaa 5761 gagccggggc ccggtttacg gccgccatga agcaacgcgc gccggtaggt ttgggaatca 5821 gggagccctc tgaataggag actgagttgg gagggaaagg ggcttcgctg ggggagcctc 5881 ggcttcttct gggagaaaat tcccacggct acctagtgag cctgcaaact ggtaggcgcc 5941 ggcgtaggcg cgcgggcggg gccgggggcg gggcctgcgg ggcgtggcgg ggcgggcaga 6001 gggcggggcc tgcttctcct cagcttcagg cggctgcgac gagccctcag gcgaacctct 6061 cggctttccc gcgcggcgcc gcctcttgct gcgcctccgc ctcctcctct gctccgccac 6121 cggcttcctc ctcctgagca gtcagcccgc gcgccggccg gctccgttat ggcgacccgc 6181 agccctggcg tcgtggtgag cagctcggcc tgccggccct ggccggttca ggcccacgcg 6241 gcaggtggcg gccgggccct gaggcgcggg atcgatccat gacacccccc cgccccccat 6301 atctcactat aattcactgt caaatggctt ttatgatttg gagtttgggg atacttttaa 6361 agacagggtc ttggtagctc ctagatggct tgggattcac ttagtaaccc ttgctggcct 6421 aaaattcagg aacttctacc tcatcttccc aagtactggg ttagtaggga tgtccagcat 6481 atttagcttg ctttaatgat tttgctgtac ttttttctgt gtgtacaggt gcatgtatat 6541 ttataaaact aacataatat attttacccc caaagtacca taaccctaat taatgctatt 6601 aaatgttttg tatgctgggc tctgggcata ctaatataac caatattata caatgcaaaa 6661 caattttgga atatcttctg taaaattatc ttattagtaa gttgtaacca tctcagactt 6721 ctaagtttga gtttgtaaag gatcagtttt ttttggtgac ctacacacac acacacacac 6781 acacacacac acacacacac aggggggtgg attctgagaa tttagagaat ttagatggtt 6841 aataccagtt tttattttta ttctgtgtga tatttattta ttaagattta tttatttatt 6901 acatgtaggt acactgtatc tgtcttcaga cacaccagaa gagggcatca gatctcgtta 6961 cagatggttg tgagtcacca tgtggttgct gggatttgaa ctcaggacct ctggaagaac 7021 agtcagtgct cttaaccgtt gagccatctc accagccccg tgatatttta tttaacttac 7081 tagaatatac tgtgttaaaa catattttag gggattgggg agatggccca ttctgtaaag 7141 tgtctgccat gtcatcttca ggacctgcgt ttgtaacccc agcatggaga agacagagac 7201 agggagaccc ctgggcttcc tggcaagcta gactagcaaa tttggttaac taggttcaat 7261 gagagactca gtctcaaaac ataagaagtg aattttttag acccaatttc aatctctgaa 7321 ctccatacgt atgcacatgt acctgcatat aaatatatat acacacccat aaacacatgc 7381 acacacaaaa aatggttcaa aagttcttaa aatgtataag agtagaaagt gtagagccgg 7441 gagtggtggc gcacgccttt aatcccagca cttgggagca gaggcaggtg gatttctgag 7501 ttcgaggcca gcctggtcta cagagtgagt tccaggacag ccagagctat acagagaaat 7561 cctgcctcaa aaaaccaaaa ataaagtgta tatactaatc tttcatgcct aaaatattta 7621 tttttgccac atttttcata cctctttaat atttttcttt ttctctttgt gcttcaaagg 7681 aaattttgtg tcatgtttta cttttcattc ttgactctat tcagcagtaa gacgcagcat 7741 ggctttctta gagaagtata atctattaca aaacggtaac atttaactgc tctacatacc 7801 ctcttgtagc taaattcctc tgttaaacta agacttatta tctaaacgta attaaatgtt 7861 gatatcttat aatgctccac tttgaaacag ctgtctttct tttgcagatt agcgatgatg 7921 aaccaggtta tgacctagat ttgttttgta tacctaatca ttatgccgag gatttggaaa 7981 aagtgtttat tcctcatgga ctgattatgg acaggttagt aagacctcga ttgaagttat 8041 ttttagaggg tgtgtgtgtg gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 8101 aagagagaga ggagaggcat gtgtgatata tatgtgaagt gtattgcatg tacatggaat 8161 ccagagaagg acattaggtg tcctactctg ttgctctctt attcccttga atcagtgtct 8221 cttgctgaac ctggggcata catactgatt tggctaggct gcctggctag caagacccag 8281 ggatctgctt gtctctgccc cccagtgctg acgttacaag caggtgcaac cacatctggt 8341 ttttatatgg gtactggggg atctgaactt agggcctgat gcgtgcccaa gtgctctcac 8401 ccgccaagcc acctctccag ccaaagggtt ttttttcttg tattaattct aacttagcaa 8461 ctgttgagta gttgatgttt gaatataagc taagccaact tattagaata aatgttttct 8521 attttttact tgtatgtgtg agtgcctgca tgtatgtatg tgaagcatat gtattccagg 8581 atgtcagaat tgtgatggat tccctggaac tggagttaca gaccattgtg aaccactgtg 8641 tatgggtgct gggaactgaa ctttaggtcc tctgcaaaaa cagtaaggat cttaactgat 8701 aaactatctt tctagcccta aggctttcta cttttaaagt tcgtttctct ttctctctct 8761 ctctctctct ctctctctct ctctccctct ccctccctcc tcctcctcct cctctttccc 8821 ccctctcgcc tcctctttct ctctcccctc cctctctctc tctcacacac acacacagac 8881 agagagagga gagagaaagg aagccttact aattaattac ctagttttag acataactag 8941 tgggaatcca tagtaagtaa atcaacattc ttgaagagag gttagaagaa ttggctctcg 9001 ttgtccatcg aggctgtata agattattct tctgttttct cagcttacca tacatatttt 9061 ctacgtagac aataccaatt caaatacact gtacgtaaag tatatgcgca cacacacaca 9121 cacacacaca cacacacaca cacaagttac atattgtcat atagtactaa aatttatgga 9181 ctttttcata ttaatacatc tagagctatc atgtttttta tcttttaaaa attactttac 9241 ggggctacag agatgtctca gcagttagga gcactgactg ctcttccaga ggtcctgagt 9301 tcaattccca gcaaccacat ggtggctcac aaccatctgt aatgggatct gatccactct 9361 tatggtgtgt ctgaagacag tgacagtgta ctcatataca tgaaataaat ctttaaaaaa 9421 ttactttacc atcagggtat atatatatat atatatgtgt gtgtgtgtgt gtgtgtgtat 9481 gaagtatttg ctatttgaga gggtctcact gtttagctca gtctggcttg gaacgttctg 9541 tatagcccag actggcctca actcagcagt ccccctgaaa ggctctcata tgtggagatt 9601 acaggtatgt gctactgtgc ccagccctac atgttacttt gtttttgaaa tagggtatca 9661 aatagcttag actggcttct aagtcagtgt gtagctaagg atggtctgag cttctgaacc 9721 tgctacttca actcctggtg tgccatcatg gccagtttgc acagttgtga gagtagaact 9781 caggacctcc tatgtgttac tagacatgtt ctctaccagc tgagctacat cccaattcgc 9841 ctacatttac ttttattttt taaaagattt atttatgtat acagcactct gcctgcatgt 9901 acatctgcat gccagaagag ggcaccagat ctcatcatac atagttgtga gccaccatgt 9961 ggttgctggg gattgaactc agggcctctg gaagagcagt cagtgctctt aacctctgag 10021 ccatctctgt agccccctac atttatttta aagattatag gctattctat tcatagatgt 10081 gccataactg atttaaccag atcttcatta ttttgtactt agtagagtat attagaggga 10141 atgaatctta aatacacatt ttgacacttg ctcagcttta gacaagtaaa aatcactggt 10201 caaggaaatg gtgcttagtg gatgcttctg aactgtcaaa ttgtcctcca acaagttaca 10261 tcattttata cttccaacgt tatgtgagag ttcatttttc tttaggctct tctagtattt 10321 tatatttgag aaataaaatt taaccaaaat ttgccaatct agtaagcttg tttttatttg 10381 tatctttaaa ttttatgata gatgttatag tgtactctcc tctcccctcc cctcccctct 10441 cctcctcagc aggatcttgt tatgtagccc taagggatct aatacttact ctgtagtcca 10501 ggctgtcctt cagctcatga gacacaccat catgcttggc atgtttcttg ttctgaaagg 10561 tatctggttt taattttggt tgagctggtg tgttggtgta tatctgtaat ctcagcacag 10621 gcaagtagag acaaggatta tgattacagt ccagcctggg ctatttagag ggattctctt 10681 taatctttcc tcatgcccca aaatcttacc tttggtatat gaaaaatagt ctccacttct 10741 gcaaaatatt gctttatgaa gtaagaatta attcccttca tagagacaag gaatgtgtcc 10801 tgtaaaagtt taatgtgtaa gaagtatttg ttataaaaga taaatattca gaatcttctt 10861 tttaattcct gattttattt ctataggact gaaagacttg ctcgagatgt catgaaggag 10921 atgggaggcc atcacattgt ggccctctgt gtgctcaagg ggggctataa gttctttgct 10981 gacctgctgg attacattaa agcactgaat agaaatagtg atagatccat tcctatgact 11041 gtagatttta tcagactgaa gagctactgt gtaagtataa ttaacttata attaaaaaaa 11101 tagggccatt ctagttttat ctatattttt tttaaacttg tgcaaactat gctacataat 11161 caacttactt gattatgtaa tatatatagt aatagttgga attactatta ttacatattt 11221 atttttctgt gctggggtag aacccaggga actaagtact gtaataaccg agcttcattc 11281 atcctagtca tttttaattt tttttttttt ttttgttttt tcgagacaag gtttctctgt 11341 gtagccctgg ctgtcctgga actcactctg tagaccaggc tggcctcgaa ctcagaaatc 11401 tgcctgcctc tgcctcccag tgctgagatt aatgacgtgc accaccactg cctggcattt 11461 ttttaaagtt ttaatttgag accctttctc actaacttgc tcacactatc ctcgaactca 11521 ttacgtgggc tggtctagaa atttcagtca acttgcctca gcttctcaag tagctgggat 11581 tacaggcagg tgctaccact tctggccatg atcattgttg ttgttgctat tattattatt 11641 attatttcac tgatacttca tatcacaaca cagaatagta actgtagttc atgaccaaca 11701 aaaaagttgt acagtttctc tgttgtctta ttttgatctg gattttgatt tgtttcatgg 11761 cactgggcag gctaatctca aactgcatgg ctcaaatagt ccttctgtct taatctccca 11821 tgttctggaa gtataggtat cactacacct agctgtattt tcatatttta aaaaaaacgc 11881 ctaagaattg ctattgaatg aggcggagag atggctcagc agtcacaagt acttgtagag 11941 gactggggtt tggttactag cacctcaaag atggcttaca attatctgta gctccaattc 12001 cagaggatct gtgtggtaaa gctgcatgta tatgggcatg caggtaaaac tgctaaacat 12061 ataaaaatag ctcagtctta agagaattgg tcgacttttt cgccctttga cgttggagtc 12121 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 12181 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 12241 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc 12301 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 12361 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 12421 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 12481 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 12541 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 12601 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 12661 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 12721 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 12781 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 12841 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 12901 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 12961 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 13021 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 13081 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 13141 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 13201 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 13261 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 13321 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 13381 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 13441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 13501 aaaccaccgc taccaggtcg agtagggata acagggtaat ggatcctcgt tgggcacctg 13561 cactcatgta cgcaggccca catatagatg tacacacgca tatcattcaa aatagaaatt 13621 atttatacaa gtagtgcgtt ggagagctgg ctcaggagtt gttaatgctt gctacccttg 13681 caaaggaccc aagttcaatt tccagcacct gtgttgggca tctcacaacc acatgtaaat 13741 ctagtttcag gggatttcac ccctcttctg aactcttcag gcacttatac ccacaaatgg 13801 acatctccac atagagacac atacttttaa aaaagtaatc tagggtctag agaaatggtt 13861 atagaccacg gactgggatc agttcccaga agccacatcc tgtggctcac aattgcctgt 13921 gaatcaagtt ctagatctgg tggcctcgtc tagcctgcag tcatcaacaa acacatgcac 13981 acatccatac acacataatt taaaagaaag cgaaagtaac ctataatgct aaacaaagca 14041 cattctcagt gtagactgca gtggtgatga accttgaaaa tatattaata gaagccagac 14101 acgtaagtca caaatggtat aactcaattt atatgaaatt cccagagagt ccaaagttaa 14161 aaaaaacaaa aaggcagatt attggttgtc agtagctaca gagatgggac attgaagata 14221 ttaataggta tagggttgag gattcatggc ctttccctct tccatgttag catgtctatt 14281 gttgttaacc ttattcaact catgttaggg caatcatatt gatgagactt aatgagtata 14341 gcttgacatt cctagaagac agagtctcac atcagactct ctgatcctct gactcttaga 14401 atctttcaac accctcttct gaaatgatct ctgagccttt ggttgtgact gaattatgag 14461 aaagaatttt atgttgaatg aattttactt cagcattttt acaaaactga agctggggat 14521 ggcgtttcat gcgtgcagtc cctaccattt gcctataagc ctggaggagg cagaagtaga 14581 attaggcttt tcagggacat cttcagctgc atcatgagct tgaggacaac ttgagctaca 14641 taagattccc atccccaccc ccacacacac acacacatct agtatgtacc tagtatgcag 14701 tcttttcata tgtttaaaat gtagaaaggt atatgtgaag tctccctttt ccccacaact 14761 tagtccctcc aaagtattgc atcagtttcc ttgtacatga ttttatgcat atataagata 14821 cactatttct ctctctctct ctctccttcc ctccttccct ccaacctctg ggtatgagtt 14881 gagggttatg tatgtttcaa gttgtaataa gaattgccac tcaaacattt catcccattt 14941 taagttaagt ttgtctgatt ttccacttta gaagggcagc catgatggct catgcctata 15001 atcccatcct agcacttgta agcctaatac ataagactgg aggattgtca tcagcttgag 15061 gctagcctgg tctacctaat gagttccagc cagccaagag tagaatataa gaccctgtct 15121 ccaaaaaatg taccaaaggg gctggagggg taactcagtg gttaagaaca gtagctgctc 15181 ttccagagga cctgggttca attcccagca cccacatggc agcttgcaac tatctgtaac 15241 tgtccagctt tgggatatcc gacaccatta taccaattac ataaaatgaa aaaattgtaa 15301 aatgtgctaa aatgagtggg gagggggagc atggtgtggt tctgtgggct tgggattttg 15361 ccatgaatcc atgactatat ctcagcaata tgtggattat ttctggaaac ctgtggggca 15421 tgggtgtatg ggaagagggg agagagagag agcccgcatc cagccagagt tcctgtgctc 15481 tgggcaggcg gacgtgggag gactgctgga cgatttccac ttggccctgg gtgggcatct 15541 ggctgtgtga agccactgac cccactcagc ggggggtaga taaggggcat ttcccatacc 15601 aggttctcag actccagcat tccacgctgg atacagcaga agagctgaga acagaatagg 15661 ggccccaggg agacactctc gtcccagaga taaagaaggc agagggagag agattcctac 15721 gcaggcgaga ggccttagtc tgggccttga tgaacagaga cggtctatgg ttttagagct 15781 ttattgtaga aaggcagggg gaaagagaga aggtagaaag agagagacca gccatggcca 15841 agaggagaga aggggggaaa ggagagagag aagaaaggct agagagtaag aaaggttaaa 15901 gagacagaag taaaagctta gagtaagaga gagagagaga gcaagaggga gagtaagaaa 15961 agtaaaagct taagagagcg aggaggggcc agcccctcta atagtgggct tgttatcttg 16021 ttgttgctag gtaactgtga agcgtctagc ctgaaggcca gaagcttggg acattgtcta 16081 tgtgactgaa agccatgagc ctctactgtg ggggctgtgg aggtggtaac ttggacagga 16141 gccaggggtt ccaggagaca tgagggaatg ccttctgtcc catgtaggta ggaattacca 16201 ccaccggctt tcaccgtagt tcactggaga ctgtttgtac atagcccatt gccccacaga 16261 aaccaggcac tcactgtttc atttgcataa gaattagaag gctttatttt ggctgggatt 16321 ggtgacttgc cccagaaatt ccagcttttg ggaagccgaa tcctggagaa ccaggagttc 16381 aaggtcatcc tcaggtacct catttgttca agaccagcct gtgctaccat gagaccttgt 16441 ccctactccc ctcaacccca aaacacgctg ttttcatttt ataggttgta ctaaccatgg 16501 aggatggtca tttgcctttc tcttgatttc agaagcgtag cgcgggcttt gcaccatgat 16561 ctagtgtttc cagataggct tgcctctcca cagccaggct tatcctgctt cagtcccatg 16621 tttggcaagg gcaacatatg actgaagaac ttgcatagcc ttatggtgta tcatgactct 16681 tagccccagt ttataactgt ctaacagctg gtggcttttg tgaaaaagga aagcatcatt 16741 tgaactttta acttttcagc tgtttgtttt gactttgcat aaatactgag actagctagg 16801 aaatgagctt ttgtttcatt tgctaaaaat gatttttttt tcatgaggca atttcaagca 16861 atttgaattt agcaatctct acttgtctga aaagaagtta ttaattaaaa aaaaacaaac 16921 aaacaggaac ctattatgct ggctagtcac atgaaagaat ccaaatgaac acagtcaagg 16981 ctgaatcaac acatgaaatt attttctaat tccccaaatt acatttatgc taattagtat 17041 gtagtaaaac attttaattg tttccaatgt taaacattac agaaataatt ttgatgtttt 17101 cctaataacc cagcctttga tcatgtttgc atttatttga ttgttgtcag tttattaaat 17161 aattaagtaa gagcaacaag ataaaatgac ttcatgagag tccttttctg taacttagaa 17221 cgtcagtagt cataggaact gcggtcgttc ccttaccttt cagaagtagg ctaaagagtt 17281 gaacgcaaag gtggtttttc gaatcttcct gatccttaag cccccactag gtttatattc 17341 tatatttgat atcggattcg aacccctcga gcttaggatc cggaaccctt aatataacta 17401 agttattagg tccctcgaag aggttcacta gtactggcca ttgcggccgc ttcgaggctg 17461 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 17521 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 17581 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac 17641 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 17701 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 17761 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 17821 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 17881 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 17941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 18001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 18061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 18121 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 18181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 18241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 18301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 18361 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 18421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 18481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 18541 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 18601 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 18661 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 18721 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 18781 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 18841 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 18901 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 18961 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 19021 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 19081 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 19141 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 19201 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 19261 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 19321 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 19381 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 19441 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 19501 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 19561 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 19621 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgg //