LOCUS Ple174\(pEMS1413 19892 bp DNA circular 3-FEB-2010 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2547 - 5' GGCTGTGGTAATGGGGGACA - 3' Tm=56.7 Deg C Antisense primer: oEMS2643 - 5' ATGCTGGTCTGAGGTTGGGG - 3 Tm=56.1 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 976 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|543239041| COMMENT VNTDBDATE|544982928| COMMENT LSOWNER| COMMENT VNTNAME|Ple174 (pEMS1413)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "Ple174 (pEMS1413)" 0 19892 COMMENT (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList) COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 19892 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) COMMENT (CObList COMMENT #0=(CFSignal (CObList) "5' homology sequence" 86 0 0 13755 17566 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "13755..17566") COMMENT #1=(CFSignal (CObList) "AmpR" 22 0 1 18665 19886 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(18665..19886)") COMMENT #2=(CFSignal (CObList) "AmpR" 22 0 0 12304 13729 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "12304..13729") COMMENT #3=(CFSignal (CObList) "3' homology sequence" 86 0 0 8663 12308 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "8663..12308") COMMENT #4=(CFSignal (CObList) "HPRT complementary sequence" 86 0 0 4709 8662 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4709..8662") COMMENT #5=(CFSignal (CObList) "I-Sce I" 21 0 0 13736 13753 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "13736..13753") COMMENT #6=(CFSignal (CObList) "MFRT (F5mutant)" 21 0 0 3591 3624 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3591..3624") COMMENT #7=(CFSignal (CObList) "Kozak consensus" 21 0 0 3625 3635 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3625..3635") COMMENT #8=(CFSignal (CObList) "EGFP" 21 0 0 3632 4351 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3632..4351") COMMENT #9=(CFSignal (CObList) "Tetracysteine residue" 21 0 0 4352 4387 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4352..4387") COMMENT #10=(CFSignal (CObList) "NLS" 21 0 0 4388 4460 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4388..4460") COMMENT #11=(CFSignal (CObList) "MFRT" 21 0 0 4466 4499 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "4466..4499") COMMENT #12=(CFSignal (CObList) "SV40 early polyA signal" 21 0 0 4653 4703 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "4653..4703") COMMENT #13=(CFSignal (CObList) "Ple174 (RAMP3-D)" 21 0 0 139 3568 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "139..3568") COMMENT #14=(CFSignal (CObList) "oEMS2643" 27 0 0 2334 2353 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #15=(CFSignal (CObList) "oEMS2547" 27 0 0 1378 1397 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "Prom Region" 21 0 0 1591 3568 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) "Region 4" 21 0 0 1088 1590 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) "Region 3" 21 0 0 139 1087 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #19=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #20=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) COMMENT "Ple174 (pEMS1413)" 2) 5 "" 0 4) COMMENT #21=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'Ple174 (pEMS1413)'" 1) COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Currently local object. Original author: Demo User" 1) COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Created: 01/15/10 11:44AM" 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 02/03/10 04:08PM" 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 19892 bp" 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #29=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 15-JAN-2010" 1))) COMMENT "Standard Fields") COMMENT #32=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Demo User" COMMENT 1))) "Author") COMMENT #34=(CFolderPar COMMENT (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Demo User" COMMENT 1))) "Original Author") COMMENT #36=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #37=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #38=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #39=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #40=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 139 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #41=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 139 End: 3568" 1) COMMENT #42=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) "1306" COMMENT 1) COMMENT #43=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #44=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 139..3568" 1))) "Ple174 (RAMP3-D)") COMMENT #45=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 139 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #46=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 139 End: 1087" 1))) "Region 3") COMMENT #47=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1088 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1088 End: 1590" 1))) "Region 4") COMMENT #49=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1591 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #50=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1591 End: 3568" 1))) COMMENT "Prom Region") COMMENT #51=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3591 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3591 End: 3624" 1) COMMENT #53=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3591..3624" 1))) "MFRT (F5mutant)") COMMENT #55=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3625 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3625 End: 3635" 1) COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3625..3635" 1))) "Kozak consensus") COMMENT #59=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3632 (3 #8# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3632 End: 4351" 1) COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 3632..4351" 1))) "EGFP") COMMENT #63=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4352 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4352 End: 4387" 1) COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4352..4387" 1))) COMMENT "Tetracysteine residue") COMMENT #67=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4388 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4388 End: 4460" 1) COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4388..4460" 1))) "NLS") COMMENT #71=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4466 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4466 End: 4499" 1) COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4466..4499" 1))) "MFRT") COMMENT #75=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4653 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4653 End: 4703" 1) COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4653..4703" 1))) COMMENT "SV40 early polyA signal") COMMENT #79=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 13736 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 13736 End: 13753" 1) COMMENT #81=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 13736..13753" 1))) "I-Sce I"))) COMMENT "Misc. Feature (12 total)") COMMENT #83=(CFolderPar COMMENT (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #84=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 12304 (3 #2# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #85=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 12304 End: 13729" 1) COMMENT #86=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 12304..13729" 1))) "AmpR") COMMENT #88=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 18665 (3 #1# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 18665 End: 19886 (Complementary)" COMMENT 1) COMMENT #90=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(18665..19886)" 1))) COMMENT "AmpR"))) "Misc. Marker (2 total)") COMMENT #92=(CFolderPar COMMENT (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #93=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1378 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #94=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1378 End: 1397" 1))) "oEMS2547") COMMENT #95=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2334 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #96=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2334 End: 2353" 1))) "oEMS2643"))) COMMENT "Primer (2 total)") COMMENT #97=(CFolderPar COMMENT (CGroupPar (CParagraph 86 (7 86 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #98=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4709 (3 #4# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #99=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4709 End: 8662" 1) COMMENT #100=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" COMMENT 1) COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 4709..8662" 1))) COMMENT "HPRT complementary sequence") COMMENT #103=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 8663 (3 #3# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #104=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 8663 End: 12308" 1) COMMENT #105=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Consists of part of Intron 2, all of Exon 3, and part of Intron 3" COMMENT 1) COMMENT #106=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #107=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 8663..12308" 1))) COMMENT "3' homology sequence") COMMENT #108=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 13755 (3 #0# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #109=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 13755 End: 17566" 1) COMMENT #110=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #111=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 13755..17566" 1))) COMMENT "5' homology sequence"))) COMMENT "Misc. Recombination (3 total)"))) "Feature Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #112=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #113=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #114=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #115=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #116=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #117=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #118=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #119=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #120=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #121=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #122=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #123=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #124=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #125=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #126=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #127=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #128=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #129=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #130=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #131=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #132=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #133=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #134=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #135=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #136=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #137=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #138=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #139=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #140=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #141=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #142=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #143=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #144=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #145=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #146=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #147=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #148=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #149=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #150=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #151=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) COMMENT 1 1 0 1 1 COMMENT (mapper: 23.3134 -14.5217 40.0378 40.0378 0.01 10 -1 19892 19892 1 0 0) COMMENT #152=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -883 100) COMMENT (CObjectList COMMENT #153=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -953 100) COMMENT (CObjectList COMMENT #154=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #118# 47828340 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.2745 6.2725 1 0.0214037) 0.0315362) COMMENT #155=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #115# 30372192 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "Ple174 (pEMS1413)" "@N" 1 0 COMMENT 0.871165 0 -10 11.4392 1.47361 Nil) COMMENT #156=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #114# 21876868 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "19892 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 3.19698 0.949103 Nil)) (CObjectList)) COMMENT #157=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1092 100) COMMENT (CObjectList COMMENT #158=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -325 100) COMMENT (CObjectList COMMENT #159=(CScratch COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #121# 653 100) COMMENT (LOGPEN 0 8 13395507) 10 1 1.94169 1.9 COMMENT 0.082322 1) COMMENT #160=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 28364812 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "I-Sce I" "@N" 1 COMMENT -12.8791 -3.48466 0 -10 3.02214 1.17389 #159#) COMMENT #161=(CScratch COMMENT (CWidget 0 (3 #6# 0) 1 2 0 0 #121# 797 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.14103 1.9 COMMENT 0.082322 1) COMMENT #162=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 21894764 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "MFRT (F5mutant)" "@N" 1 15.4452 1.74233 0 COMMENT -10 7.91752 1.17389 #161#) COMMENT #163=(CScratch COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #121# 797 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.13031 1.9 COMMENT 0.082322 1) COMMENT #164=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 21586468 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Kozak consensus" "@N" 1 15.5475 0 0 -10 COMMENT 7.99245 1.17389 #163#) COMMENT #165=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #8# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 4.90104 COMMENT 5.1281 1 0.082322) 0.8 1.8 0) COMMENT #166=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 2082602612 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "EGFP" "@N" 1 COMMENT 13.0756 -1.74233 0 -10 2.67247 1.17389 #165#) COMMENT #167=(CScratch COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #121# 652 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.90104 1.9 COMMENT 0.082322 1) COMMENT #168=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 4063824 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Tetracysteine residue" "@N" 1 16.2775 COMMENT -3.48466 0 -10 10.0405 1.17389 #167#) COMMENT #169=(CScratch COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #121# 718 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.88969 1.9 COMMENT 0.082322 1) COMMENT #170=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 2082602612 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 COMMENT 12.8934 -5.22699 0 -10 1.99811 1.17389 #169#) COMMENT #171=(CScratch COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #121# 718 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.86509 1.9 COMMENT 0.082322 1) COMMENT #172=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 4063648 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "MFRT" "@N" 1 COMMENT 13.4095 -6.96932 0 -10 2.72243 1.17389 #171#) COMMENT #173=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #121# 828 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.80612 1.9 COMMENT 0.082322 1) COMMENT #174=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 21520428 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "SV40 early polyA signal" "@N" 1 17.2686 COMMENT -8.71165 0 -10 11.0645 1.17389 #173#) COMMENT #175=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 5.14797 COMMENT 6.22967 1 0.082322) 0.8 1.8 0) COMMENT #176=(CLabel (CWidget 0 (0 0) 1 2 0 0 #113# 6 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Ple174 (RAMP3-D)" "@N" 1 13.8384 6.96932 0 COMMENT -10 8.29216 1.17389 #175#) COMMENT #177=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.670712 5.14797 COMMENT 5.77176 1 0.082322) 0.8 1.8 0) COMMENT #178=(CLabel (CWidget 0 (0 0) 1 2 0 0 #113# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Prom Region" COMMENT "@N" 1 14.2643 3.48466 0 -10 6.16917 1.17389 COMMENT #177#) COMMENT #179=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 5.77176 5.93039 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #180=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 4063824 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Region 4" "@N" COMMENT 1 8.32038 10.454 0 -10 4.19603 1.17389 #179#) COMMENT #181=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 5.93039 6.22967 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #182=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 27868012 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Region 3" "@N" COMMENT 1 5.02909 12.1963 0 -10 4.19603 1.17389 #181#)) COMMENT (CObjectList)) COMMENT #183=(CGroupWidget COMMENT (CWidget 22 (7 22 0) 1 2 0 0 Nil -185 100) COMMENT (CObjectList COMMENT #184=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #1# 0) 1 2 0 0 #129# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 COMMENT 0.00189217 0.387265 1 0.082322) 0.8 1.8 1) COMMENT #185=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 21894764 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -4.39695 12.1963 0 -10 2.87229 1.17389 #184#) COMMENT #186=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #2# 0) 1 2 0 0 #129# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 COMMENT 1.94358 2.39328 1 0.082322) 0.8 1.8 0) COMMENT #187=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 21517476 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -11.5515 -6.96932 0 -10 2.87229 1.17389 #186#)) COMMENT (CObjectList)) COMMENT #188=(CGroupWidget COMMENT (CWidget 27 (7 27 0) 1 2 0 0 Nil -883 100) COMMENT (CObjectList COMMENT #189=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #121# 828 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.53745 1.9 COMMENT 0.082322 1) COMMENT #190=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 7258160 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "oEMS2643" "@N" COMMENT 1 13.126 5.22699 0 -10 4.79547 1.17389 #189#) COMMENT #191=(CScratch COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #121# 816 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.83893 1.9 COMMENT 0.082322 1) COMMENT #192=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 24492572 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "oEMS2547" "@N" COMMENT 1 10.8535 8.71165 0 -10 4.79547 1.17389 #191#)) COMMENT (CObjectList)) COMMENT #193=(CGroupWidget COMMENT (CWidget 86 (7 86 0) 1 2 0 0 Nil -464 100) COMMENT (CObjectList COMMENT #194=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #0# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 0.733533 COMMENT 1.93569 1 0.082322) 0.8 1.8 0) COMMENT #195=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 21874452 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "5' homology sequence" "@N" 1 -16.3143 COMMENT 3.48466 0 -10 10.4901 1.17389 #194#) COMMENT #196=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #3# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.39171 3.54152 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #197=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 4063824 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "3' homology sequence" "@N" 1 -7.43311 COMMENT -12.1963 0 -10 10.4901 1.17389 #196#) COMMENT #198=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 3.54152 COMMENT 4.78846 1 0.082322) 0.8 1.8 0) COMMENT #199=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #113# 4063824 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "HPRT complementary sequence" "@N" 1 17.3734 COMMENT -10.454 0 -10 14.9609 1.17389 #198#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #200=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -116 100) COMMENT (CObjectList) (CObjectList)) COMMENT #201=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -116 100) COMMENT (CObjectList) (CObjectList)) COMMENT #202=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1077 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) COMMENT (CObList #203=(AnlzFontColorItem 2333 2352 41943168) COMMENT #204=(AnlzFontColorItem 1377 1396 41943168)) 1 COMMENT (CObList #205=(AnlzFontBackColorItem 1590 3567 33554687) COMMENT #206=(AnlzFontBackColorItem 138 1086 33619712) COMMENT #207=(AnlzFontBackColorItem 1087 1589 50331392))) (CObList) 0 COMMENT (CStringList) 0 1684625219 (CObList))) FEATURES Location/Qualifiers misc_recomb 13755..17566 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(18665..19886) /vntifkey="22" /label=AmpR misc_marker 12304..13729 /vntifkey="22" /label=AmpR misc_recomb 8663..12308 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4709..8662 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 13736..13753 /vntifkey="21" /label=I-Sce\I misc_feature 3591..3624 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 3625..3635 /vntifkey="21" /label=Kozak\consensus misc_feature 3632..4351 /vntifkey="21" /label=EGFP misc_feature 4352..4387 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4388..4460 /vntifkey="21" /label=NLS misc_feature 4466..4499 /vntifkey="21" /label=MFRT misc_feature 4653..4703 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..3568 /vntifkey="21" /label=Ple174\(RAMP3-D) /note="1306" primer 2334..2353 /vntifkey="27" /label=oEMS2643 primer 1378..1397 /vntifkey="27" /label=oEMS2547 misc_feature 1591..3568 /vntifkey="21" /label=Prom\Region misc_feature 1088..1590 /vntifkey="21" /label=Region\4 misc_feature 139..1087 /vntifkey="21" /label=Region\3 BASE COUNT 5165 a 4693 c 4586 g 5448 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccct ggtgacaaag cgagactctg cctcaaaaaa aaaaaaaaaa 181 aaaaaaaaaa aaaaaagact tgaaggtggt gattggccaa gaagaggccc atagattttt 241 ggcactggag gttccttgga ggttatctga cctccatgct cttacctatt cttcataagg 301 aaacagaaat ctggaggtta gagaggaagt tgcccaacca cagagaccag gggcagaacc 361 gcccagaatc cacagcctcc caaggtcgac tgaccgtggc ccatggcagc cacttgccag 421 tctcgtcgat gctctttctg tcaaggaggg ggagagcgcc tgtgtttcat ttccaaatga 481 ctagccacaa aagccctttc tggcggaaat gcatctgttt ggctgtttat catctctcag 541 tggcgtctgg aagtccctga gacaaaagaa acacaggccg gcatgttctt tgtttcccaa 601 tgaaaccaac caaaatggat ttctattttg attcagttcc tgcgaccttt actaatgcag 661 ccagtagctt gattggcaat ggccctcttt tgtggtgttg ccaggaatct gtctttacac 721 cattgttcta acattgggtg ggttgtcagg ttttgctggg agcccctcaa gcctcatgtg 781 cactagaaat gagctccctg gggagggcac ttggtttctc agggtgcctg ccttgtgcag 841 gcccagggct ggtgctgtca caggcatcct catagcacct ccgtgggtag aggagttatc 901 ccagaacacc tgcctgagga tggcatgtgg tcctcaaagg cacatttaca atctgcctga 961 gccaggtcct tgcgatgggg catgtgaaga ttatggagta tgtagagatt tggggcatgc 1021 agagattcag ggaggggcat gttgtcaggg acccctcctt ctctttttag atagctgtgc 1081 gacctgggcc tattgtgcag ttgactctgc tgggtctgtc ctatctctgg tggctgccct 1141 gaaagatgac agtgcccaga ggaactaggg atagagcccc caagcctcag ggctttctgg 1201 gtgggttcat gaatggtggt ccctaccagc cttatagaag aatggcttca cttttgagtc 1261 tcttattact ttttggttcc ttcaaagcac ttcagggtct agagctgtgc tacaagagaa 1321 cctggggtca gcagcttggg atacacagaa gtgggagctg ctggtgtcca ggaaggaggc 1381 tgtggtaatg ggggacagtc tgattgtgga ggaagagggg caggtgctgc cagcagccag 1441 agggtcgtgg agcagcagct catgggtggg acaagctgga ggctggggtc agcagtggtg 1501 ggaataggaa gttggggccc gatggaagct aaccttgaca gagattttgt aggtgcacaa 1561 ttattaacca atggaaacac acagaatcag ggcagtagac acaactccct gggagtttgt 1621 tggggcatgg ttggttccat ggaggagagg tgttggaacc caactctgta gaatggccag 1681 ggcttcccca ctgcagagcc agggtgggga gtccaggaaa gggagcattg ctccctggtg 1741 caggcttggg ggaactggct gcccccctcc cacctgacaa ccccatccta ctgttccagc 1801 ctgcaagcta tgtgcaaccg cctttggggc tgtgggaccg agtggagact aagactctcc 1861 tggagagagc tgtagggccg agtctgtgtg ggatgtgggt ggacacctgg gcctggacca 1921 tcctcttggt ttgtcttggc tggtgtaggg tgtgcgtgtg tgtaagccag ccccctgctg 1981 ccctggtcag cttgttttat gacccctggc ctgtgggcag aaggcctggc tgtgggcacc 2041 atcagtctca ctagaggctc tgtggccacc cctacacctg ctgcccaccc ctcaggcaga 2101 aggcaggaca aatgatcaat tttaattagt tttgactcct ttgaattaat tctggctgta 2161 atcagaattt cttcagtaaa aaagcacaga aagttcttct cccgaataag ggtcttgtta 2221 aataaagtgg gtctttctaa gccaggagaa gttcagctca gctcctggga aggtagcagt 2281 acctacggct cagaggagtg gagggcccat ccatgtagga gcctgggcct gtgccccaac 2341 ctcagaccag catgtgcccc caggggtggt gggtgggccc tggagggcag agaggtgcct 2401 ctagctggct ctcggaagtc ctgaccagtg acaattctta agacaccatt aagtgtccac 2461 cggagccagg gcctgtgcta ggggctgagg gtgacagagg tgacccatct ggccaagtga 2521 cctgtggctg gagaaactga ggccctcctt cgcctcaggg accctgacac tcttcaaggc 2581 caggtctttg gtggtgccag atactctcct gtcccttggt ttgcttgtgg cttggttctt 2641 ggtgtgtgat acgtctctgt gtgtgtagtt gccacaccct ccagtctctc tcatacacac 2701 tgtttcccaa gcatacactc acaaacacac cttgtctcag actcaaacac acacatgcca 2761 cacacacacc atacatcaca cactccatgc acacacatgc atctacacac accccacatg 2821 cacatcacac acacgtactc catgaacaac acacacacgc acacacacag acgcacacac 2881 acacaccccc caacctcttt tatgcacttt cctgaccttt ggagacctgg ctcctagatg 2941 tatctctgct caaagttccc ctccctgcct gggggcagct ctggactctg gtctactttc 3001 ggccagggca cacctagccc actcccaggt ccaccttcct ccagtctacc cccagactcc 3061 tctaggccat catcagccag cgcttcatcc tcacctcagc tccctccccg gtctaccccc 3121 accctctgcc cctgagcctg gacggaagtt ccccaagtct ctccagcctg catcccagcc 3181 caccgtcagc cggcccccac gctcacccca gctcacctcc cagtctgccc ctacaatctg 3241 ctcccaaaaa gctcctttgc agctcagcac cccagccccg ccctcagagc ccatcagccg 3301 gcaccccacc ttaccccagc ttgtccccag ctcgaaaccc tgccctcgcc cccctctcac 3361 cccgctgaga ctccagccgc ccccatccct cccacccggc gcgcctgcag ggactgcacc 3421 ccacatctgg aggcaacggg gcacggccgc aggcgtggtc tgggggtgcg gcctgagggc 3481 ggaccccctg aggccccgcc ccaccccaac cccaaccggg cgtcccccag ccgcgccccc 3541 agcgggaccg agcgtgaccc agctgcgggg ccggccacgc gtggcgcgcc gaagttccta 3601 ttcttcaaaa ggtataggaa cttccgccac catggtgagc aagggcgagg agctgttcac 3661 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 3721 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 3781 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 3841 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 3901 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 3961 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 4021 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 4081 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 4141 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 4201 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 4261 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 4321 cactctcggc atggacgagc tgtacaagtt atttctgaac tgctgcccgg gctgctgcat 4381 ggaaccggat ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtagatcc 4441 aaaaaagaag agaaaggtaa tataagaagt tcctattctc tagaaagtat aggaacttca 4501 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt 4561 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 4621 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 4681 aaataaagca tttttttcac tgcgaattct cgtaaaactc ttcatggcag tagttattat 4741 tctctctctc tctctttttc ttttttcttg agacaggata tttctctgtt gcccaggctg 4801 gagtgcagtg gcacagtctt ggctcactgc agcctggacc tcctgggctc aagccatcct 4861 cccacctcag cctcccaagt agctggggct acaggcacat ggccaccagg cccagataat 4921 ttttcatttt tgtagagact gagtctcacc atgttaccca ggtttattat tctcattttt 4981 tagatgaaga gactgaggtc cagagaagct caatgacttg cctagtttta caaatctcct 5041 gccatcacat acccctcagc gtccttaata agagggaggc caccaactat gtgctgggca 5101 ctgtggtgga tgctggagct atagggttga gtatataaga aatggtgttg ctggagcaac 5161 tgttgcttgc ttacctgacc tatctgagaa ttaattagca ggggaacata tttttgtttt 5221 cagattcaat ataagaactt gtgtgggcaa aaataaagat cagtagtaat aacagtagtt 5281 cccatttgct gactgtactg tcctaagtgc atatatatat acatacacac acgcatacct 5341 atactcctct aatactcaaa atgatcctgt ttatgtattg ttaatatgct cattttattt 5401 ttaaattttt atttattttt atttttattt atttttgaga cggagtctca ttctgtcgcg 5461 gaggctgaag tgcagtggtg cgatctcagc tcagtgcgac ctccgcctcc cgggttcaag 5521 tgattctcct gcctcagcct ccggactagc tgggattaca ggcgcccgcc tccacgccca 5581 gctaattttt gtatttttag tagagatggg gtttcgccat gttggccagg ctggtctcgt 5641 actcctgacc ttgagtgatc cacctgcctc ggcctcccaa agtgctggga ttacaggcat 5701 gagccaccgc gccgggctaa tatgctcatt ttagtgaggc aaaaatagag gctcagagtc 5761 tgatttgtac aaaactacag agcagttaag tgtcctctca gatgtgtacc ctgatctggg 5821 tgactctagg actctaggtc tcaactgtta caaccagtta agggtttggg gaagcactgg 5881 gccaagagtc aggaaaatgg aagccacagg tagtgcaagg tcttgggaat gggacgtctg 5941 gtccaaggat tcacgcgatg actggaaccc gaagagccgg ggcccggttt acggccgcca 6001 tgaagcaacg cgcgccggta ggtttgggaa tcagggagcc ctctgaatag gagactgagt 6061 tgggagggaa aggggcttcg ctgggggagc ctcggcttct tctgggagaa aattcccacg 6121 gctacctagt gagcctgcaa actggtaggc gccggcgtag gcgcgcgggc ggggccgggg 6181 gcggggcctg cggggcgtgg cggggcgggc agagggcggg gcctgcttct cctcagcttc 6241 aggcggctgc gacgagccct caggcgaacc tctcggcttt cccgcgcggc gccgcctctt 6301 gctgcgcctc cgcctcctcc tctgctccgc caccggcttc ctcctcctga gcagtcagcc 6361 cgcgcgccgg ccggctccgt tatggcgacc cgcagccctg gcgtcgtggt gagcagctcg 6421 gcctgccggc cctggccggt tcaggcccac gcggcaggtg gcggccgggc cctgaggcgc 6481 gggatcgatc catgacaccc ccccgccccc catatctcac tataattcac tgtcaaatgg 6541 cttttatgat ttggagtttg gggatacttt taaagacagg gtcttggtag ctcctagatg 6601 gcttgggatt cacttagtaa cccttgctgg cctaaaattc aggaacttct acctcatctt 6661 cccaagtact gggttagtag ggatgtccag catatttagc ttgctttaat gattttgctg 6721 tacttttttc tgtgtgtaca ggtgcatgta tatttataaa actaacataa tatattttac 6781 ccccaaagta ccataaccct aattaatgct attaaatgtt ttgtatgctg ggctctgggc 6841 atactaatat aaccaatatt atacaatgca aaacaatttt ggaatatctt ctgtaaaatt 6901 atcttattag taagttgtaa ccatctcaga cttctaagtt tgagtttgta aaggatcagt 6961 tttttttggt gacctacaca cacacacaca cacacacaca cacacacaca cacagggggg 7021 tggattctga gaatttagag aatttagatg gttaatacca gtttttattt ttattctgtg 7081 tgatatttat ttattaagat ttatttattt attacatgta ggtacactgt atctgtcttc 7141 agacacacca gaagagggca tcagatctcg ttacagatgg ttgtgagtca ccatgtggtt 7201 gctgggattt gaactcagga cctctggaag aacagtcagt gctcttaacc gttgagccat 7261 ctcaccagcc ccgtgatatt ttatttaact tactagaata tactgtgtta aaacatattt 7321 taggggattg gggagatggc ccattctgta aagtgtctgc catgtcatct tcaggacctg 7381 cgtttgtaac cccagcatgg agaagacaga gacagggaga cccctgggct tcctggcaag 7441 ctagactagc aaatttggtt aactaggttc aatgagagac tcagtctcaa aacataagaa 7501 gtgaattttt tagacccaat ttcaatctct gaactccata cgtatgcaca tgtacctgca 7561 tataaatata tatacacacc cataaacaca tgcacacaca aaaaatggtt caaaagttct 7621 taaaatgtat aagagtagaa agtgtagagc cgggagtggt ggcgcacgcc tttaatccca 7681 gcacttggga gcagaggcag gtggatttct gagttcgagg ccagcctggt ctacagagtg 7741 agttccagga cagccagagc tatacagaga aatcctgcct caaaaaacca aaaataaagt 7801 gtatatacta atctttcatg cctaaaatat ttatttttgc cacatttttc atacctcttt 7861 aatatttttc tttttctctt tgtgcttcaa aggaaatttt gtgtcatgtt ttacttttca 7921 ttcttgactc tattcagcag taagacgcag catggctttc ttagagaagt ataatctatt 7981 acaaaacggt aacatttaac tgctctacat accctcttgt agctaaattc ctctgttaaa 8041 ctaagactta ttatctaaac gtaattaaat gttgatatct tataatgctc cactttgaaa 8101 cagctgtctt tcttttgcag attagcgatg atgaaccagg ttatgaccta gatttgtttt 8161 gtatacctaa tcattatgcc gaggatttgg aaaaagtgtt tattcctcat ggactgatta 8221 tggacaggtt agtaagacct cgattgaagt tatttttaga gggtgtgtgt gtggtgtgtg 8281 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtaagagag agaggagagg catgtgtgat 8341 atatatgtga agtgtattgc atgtacatgg aatccagaga aggacattag gtgtcctact 8401 ctgttgctct cttattccct tgaatcagtg tctcttgctg aacctggggc atacatactg 8461 atttggctag gctgcctggc tagcaagacc cagggatctg cttgtctctg ccccccagtg 8521 ctgacgttac aagcaggtgc aaccacatct ggtttttata tgggtactgg gggatctgaa 8581 cttagggcct gatgcgtgcc caagtgctct cacccgccaa gccacctctc cagccaaagg 8641 gttttttttc ttgtattaat tctaacttag caactgttga gtagttgatg tttgaatata 8701 agctaagcca acttattaga ataaatgttt tctatttttt acttgtatgt gtgagtgcct 8761 gcatgtatgt atgtgaagca tatgtattcc aggatgtcag aattgtgatg gattccctgg 8821 aactggagtt acagaccatt gtgaaccact gtgtatgggt gctgggaact gaactttagg 8881 tcctctgcaa aaacagtaag gatcttaact gataaactat ctttctagcc ctaaggcttt 8941 ctacttttaa agttcgtttc tctttctctc tctctctctc tctctctctc tctctctccc 9001 tctccctccc tcctcctcct cctcctcttt cccccctctc gcctcctctt tctctctccc 9061 ctccctctct ctctctcaca cacacacaca gacagagaga ggagagagaa aggaagcctt 9121 actaattaat tacctagttt tagacataac tagtgggaat ccatagtaag taaatcaaca 9181 ttcttgaaga gaggttagaa gaattggctc tcgttgtcca tcgaggctgt ataagattat 9241 tcttctgttt tctcagctta ccatacatat tttctacgta gacaatacca attcaaatac 9301 actgtacgta aagtatatgc gcacacacac acacacacac acacacacac acacacaagt 9361 tacatattgt catatagtac taaaatttat ggactttttc atattaatac atctagagct 9421 atcatgtttt ttatctttta aaaattactt tacggggcta cagagatgtc tcagcagtta 9481 ggagcactga ctgctcttcc agaggtcctg agttcaattc ccagcaacca catggtggct 9541 cacaaccatc tgtaatggga tctgatccac tcttatggtg tgtctgaaga cagtgacagt 9601 gtactcatat acatgaaata aatctttaaa aaattacttt accatcaggg tatatatata 9661 tatatatatg tgtgtgtgtg tgtgtgtgtg tatgaagtat ttgctatttg agagggtctc 9721 actgtttagc tcagtctggc ttggaacgtt ctgtatagcc cagactggcc tcaactcagc 9781 agtccccctg aaaggctctc atatgtggag attacaggta tgtgctactg tgcccagccc 9841 tacatgttac tttgtttttg aaatagggta tcaaatagct tagactggct tctaagtcag 9901 tgtgtagcta aggatggtct gagcttctga acctgctact tcaactcctg gtgtgccatc 9961 atggccagtt tgcacagttg tgagagtaga actcaggacc tcctatgtgt tactagacat 10021 gttctctacc agctgagcta catcccaatt cgcctacatt tacttttatt ttttaaaaga 10081 tttatttatg tatacagcac tctgcctgca tgtacatctg catgccagaa gagggcacca 10141 gatctcatca tacatagttg tgagccacca tgtggttgct ggggattgaa ctcagggcct 10201 ctggaagagc agtcagtgct cttaacctct gagccatctc tgtagccccc tacatttatt 10261 ttaaagatta taggctattc tattcataga tgtgccataa ctgatttaac cagatcttca 10321 ttattttgta cttagtagag tatattagag ggaatgaatc ttaaatacac attttgacac 10381 ttgctcagct ttagacaagt aaaaatcact ggtcaaggaa atggtgctta gtggatgctt 10441 ctgaactgtc aaattgtcct ccaacaagtt acatcatttt atacttccaa cgttatgtga 10501 gagttcattt ttctttaggc tcttctagta ttttatattt gagaaataaa atttaaccaa 10561 aatttgccaa tctagtaagc ttgtttttat ttgtatcttt aaattttatg atagatgtta 10621 tagtgtactc tcctctcccc tcccctcccc tctcctcctc agcaggatct tgttatgtag 10681 ccctaaggga tctaatactt actctgtagt ccaggctgtc cttcagctca tgagacacac 10741 catcatgctt ggcatgtttc ttgttctgaa aggtatctgg ttttaatttt ggttgagctg 10801 gtgtgttggt gtatatctgt aatctcagca caggcaagta gagacaagga ttatgattac 10861 agtccagcct gggctattta gagggattct ctttaatctt tcctcatgcc ccaaaatctt 10921 acctttggta tatgaaaaat agtctccact tctgcaaaat attgctttat gaagtaagaa 10981 ttaattccct tcatagagac aaggaatgtg tcctgtaaaa gtttaatgtg taagaagtat 11041 ttgttataaa agataaatat tcagaatctt ctttttaatt cctgatttta tttctatagg 11101 actgaaagac ttgctcgaga tgtcatgaag gagatgggag gccatcacat tgtggccctc 11161 tgtgtgctca aggggggcta taagttcttt gctgacctgc tggattacat taaagcactg 11221 aatagaaata gtgatagatc cattcctatg actgtagatt ttatcagact gaagagctac 11281 tgtgtaagta taattaactt ataattaaaa aaatagggcc attctagttt tatctatatt 11341 ttttttaaac ttgtgcaaac tatgctacat aatcaactta cttgattatg taatatatat 11401 agtaatagtt ggaattacta ttattacata tttatttttc tgtgctgggg tagaacccag 11461 ggaactaagt actgtaataa ccgagcttca ttcatcctag tcatttttaa tttttttttt 11521 tttttttgtt ttttcgagac aaggtttctc tgtgtagccc tggctgtcct ggaactcact 11581 ctgtagacca ggctggcctc gaactcagaa atctgcctgc ctctgcctcc cagtgctgag 11641 attaatgacg tgcaccacca ctgcctggca tttttttaaa gttttaattt gagacccttt 11701 ctcactaact tgctcacact atcctcgaac tcattacgtg ggctggtcta gaaatttcag 11761 tcaacttgcc tcagcttctc aagtagctgg gattacaggc aggtgctacc acttctggcc 11821 atgatcattg ttgttgttgc tattattatt attattattt cactgatact tcatatcaca 11881 acacagaata gtaactgtag ttcatgacca acaaaaaagt tgtacagttt ctctgttgtc 11941 ttattttgat ctggattttg atttgtttca tggcactggg caggctaatc tcaaactgca 12001 tggctcaaat agtccttctg tcttaatctc ccatgttctg gaagtatagg tatcactaca 12061 cctagctgta ttttcatatt ttaaaaaaaa cgcctaagaa ttgctattga atgaggcgga 12121 gagatggctc agcagtcaca agtacttgta gaggactggg gtttggttac tagcacctca 12181 aagatggctt acaattatct gtagctccaa ttccagagga tctgtgtggt aaagctgcat 12241 gtatatgggc atgcaggtaa aactgctaaa catataaaaa tagctcagtc ttaagagaat 12301 tggtcgactt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 12361 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 12421 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 12481 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc 12541 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 12601 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 12661 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 12721 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 12781 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 12841 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 12901 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 12961 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 13021 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 13081 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 13141 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 13201 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 13261 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 13321 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 13381 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 13441 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 13501 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 13561 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 13621 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 13681 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagg tcgagtaggg 13741 ataacagggt aatggatcct cgttgggcac ctgcactcat gtacgcaggc ccacatatag 13801 atgtacacac gcatatcatt caaaatagaa attatttata caagtagtgc gttggagagc 13861 tggctcagga gttgttaatg cttgctaccc ttgcaaagga cccaagttca atttccagca 13921 cctgtgttgg gcatctcaca accacatgta aatctagttt caggggattt cacccctctt 13981 ctgaactctt caggcactta tacccacaaa tggacatctc cacatagaga cacatacttt 14041 taaaaaagta atctagggtc tagagaaatg gttatagacc acggactggg atcagttccc 14101 agaagccaca tcctgtggct cacaattgcc tgtgaatcaa gttctagatc tggtggcctc 14161 gtctagcctg cagtcatcaa caaacacatg cacacatcca tacacacata atttaaaaga 14221 aagcgaaagt aacctataat gctaaacaaa gcacattctc agtgtagact gcagtggtga 14281 tgaaccttga aaatatatta atagaagcca gacacgtaag tcacaaatgg tataactcaa 14341 tttatatgaa attcccagag agtccaaagt taaaaaaaac aaaaaggcag attattggtt 14401 gtcagtagct acagagatgg gacattgaag atattaatag gtatagggtt gaggattcat 14461 ggcctttccc tcttccatgt tagcatgtct attgttgtta accttattca actcatgtta 14521 gggcaatcat attgatgaga cttaatgagt atagcttgac attcctagaa gacagagtct 14581 cacatcagac tctctgatcc tctgactctt agaatctttc aacaccctct tctgaaatga 14641 tctctgagcc tttggttgtg actgaattat gagaaagaat tttatgttga atgaatttta 14701 cttcagcatt tttacaaaac tgaagctggg gatggcgttt catgcgtgca gtccctacca 14761 tttgcctata agcctggagg aggcagaagt agaattaggc ttttcaggga catcttcagc 14821 tgcatcatga gcttgaggac aacttgagct acataagatt cccatcccca cccccacaca 14881 cacacacaca tctagtatgt acctagtatg cagtcttttc atatgtttaa aatgtagaaa 14941 ggtatatgtg aagtctccct tttccccaca acttagtccc tccaaagtat tgcatcagtt 15001 tccttgtaca tgattttatg catatataag atacactatt tctctctctc tctctctcct 15061 tccctccttc cctccaacct ctgggtatga gttgagggtt atgtatgttt caagttgtaa 15121 taagaattgc cactcaaaca tttcatccca ttttaagtta agtttgtctg attttccact 15181 ttagaagggc agccatgatg gctcatgcct ataatcccat cctagcactt gtaagcctaa 15241 tacataagac tggaggattg tcatcagctt gaggctagcc tggtctacct aatgagttcc 15301 agccagccaa gagtagaata taagaccctg tctccaaaaa atgtaccaaa ggggctggag 15361 gggtaactca gtggttaaga acagtagctg ctcttccaga ggacctgggt tcaattccca 15421 gcacccacat ggcagcttgc aactatctgt aactgtccag ctttgggata tccgacacca 15481 ttataccaat tacataaaat gaaaaaattg taaaatgtgc taaaatgagt ggggaggggg 15541 agcatggtgt ggttctgtgg gcttgggatt ttgccatgaa tccatgacta tatctcagca 15601 atatgtggat tatttctgga aacctgtggg gcatgggtgt atgggaagag gggagagaga 15661 gagagcccgc atccagccag agttcctgtg ctctgggcag gcggacgtgg gaggactgct 15721 ggacgatttc cacttggccc tgggtgggca tctggctgtg tgaagccact gaccccactc 15781 agcggggggt agataagggg catttcccat accaggttct cagactccag cattccacgc 15841 tggatacagc agaagagctg agaacagaat aggggcccca gggagacact ctcgtcccag 15901 agataaagaa ggcagaggga gagagattcc tacgcaggcg agaggcctta gtctgggcct 15961 tgatgaacag agacggtcta tggttttaga gctttattgt agaaaggcag ggggaaagag 16021 agaaggtaga aagagagaga ccagccatgg ccaagaggag agaagggggg aaaggagaga 16081 gagaagaaag gctagagagt aagaaaggtt aaagagacag aagtaaaagc ttagagtaag 16141 agagagagag agagcaagag ggagagtaag aaaagtaaaa gcttaagaga gcgaggaggg 16201 gccagcccct ctaatagtgg gcttgttatc ttgttgttgc taggtaactg tgaagcgtct 16261 agcctgaagg ccagaagctt gggacattgt ctatgtgact gaaagccatg agcctctact 16321 gtgggggctg tggaggtggt aacttggaca ggagccaggg gttccaggag acatgaggga 16381 atgccttctg tcccatgtag gtaggaatta ccaccaccgg ctttcaccgt agttcactgg 16441 agactgtttg tacatagccc attgccccac agaaaccagg cactcactgt ttcatttgca 16501 taagaattag aaggctttat tttggctggg attggtgact tgccccagaa attccagctt 16561 ttgggaagcc gaatcctgga gaaccaggag ttcaaggtca tcctcaggta cctcatttgt 16621 tcaagaccag cctgtgctac catgagacct tgtccctact cccctcaacc ccaaaacacg 16681 ctgttttcat tttataggtt gtactaacca tggaggatgg tcatttgcct ttctcttgat 16741 ttcagaagcg tagcgcgggc tttgcaccat gatctagtgt ttccagatag gcttgcctct 16801 ccacagccag gcttatcctg cttcagtccc atgtttggca agggcaacat atgactgaag 16861 aacttgcata gccttatggt gtatcatgac tcttagcccc agtttataac tgtctaacag 16921 ctggtggctt ttgtgaaaaa ggaaagcatc atttgaactt ttaacttttc agctgtttgt 16981 tttgactttg cataaatact gagactagct aggaaatgag cttttgtttc atttgctaaa 17041 aatgattttt ttttcatgag gcaatttcaa gcaatttgaa tttagcaatc tctacttgtc 17101 tgaaaagaag ttattaatta aaaaaaaaca aacaaacagg aacctattat gctggctagt 17161 cacatgaaag aatccaaatg aacacagtca aggctgaatc aacacatgaa attattttct 17221 aattccccaa attacattta tgctaattag tatgtagtaa aacattttaa ttgtttccaa 17281 tgttaaacat tacagaaata attttgatgt tttcctaata acccagcctt tgatcatgtt 17341 tgcatttatt tgattgttgt cagtttatta aataattaag taagagcaac aagataaaat 17401 gacttcatga gagtcctttt ctgtaactta gaacgtcagt agtcatagga actgcggtcg 17461 ttcccttacc tttcagaagt aggctaaaga gttgaacgca aaggtggttt ttcgaatctt 17521 cctgatcctt aagcccccac taggtttata ttctatattt gatatcggat tcgaacccct 17581 cgagcttagg atccggaacc cttaatataa ctaagttatt aggtccctcg aagaggttca 17641 ctagtactgg ccattgcggc cgcttcgagg ctgcctcgcg cgtttcggtg atgacggtga 17701 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 17761 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat 17821 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag 17881 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 17941 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 18001 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 18061 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 18121 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 18181 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 18241 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 18301 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 18361 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 18421 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 18481 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 18541 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 18601 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 18661 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 18721 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 18781 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 18841 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 18901 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 18961 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 19021 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 19081 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 19141 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 19201 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 19261 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 19321 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 19381 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 19441 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 19501 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 19561 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 19621 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 19681 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 19741 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 19801 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 19861 cgcacatttc cccgaaaagt gccacctgac gg //