LOCUS Ple150\(pEMS1171 19643 bp DNA circular 23-APR-2010 SOURCE ORGANISM COMMENT PCR genotyping: AntiSense primer: oEMS2674 - TGGTCAGGCTGCAACTCACG Tm=56.9 Deg C Sense primer: oEMS2682 - TAAAGGCAACGCAAGTGCGA Tm=56.8 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 654 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|552233777| COMMENT VNTDBDATE|552233777| COMMENT LSOWNER| COMMENT VNTNAME|Ple150 (pEMS1171)| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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COMMENT 0.082322) 0.8 1.8 0) COMMENT #202=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #116# 4063648 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "3' homology sequence" "@N" 1 -6.98826 COMMENT -12.1963 0 -10 10.4901 1.17389 #201#) COMMENT #203=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #120# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 3.58641 COMMENT 4.84916 1 0.082322) 0.8 1.8 0) COMMENT #204=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #116# 22159180 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "HPRT complementary sequence" "@N" 1 17.8228 COMMENT -12.1963 0 -10 14.9609 1.17389 #203#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #205=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -116 100) COMMENT (CObjectList) (CObjectList)) COMMENT #206=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -325 100) COMMENT (CObjectList) (CObjectList)) COMMENT #207=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -464 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #208=(AnlzFontBackColorItem 2862 3333 33554687) COMMENT #209=(AnlzFontBackColorItem 1199 1950 33619712) COMMENT #210=(AnlzFontBackColorItem 1951 2861 33619967) COMMENT #211=(AnlzFontBackColorItem 138 1198 50331392))) (CObList) 34275824 COMMENT (CStringList) 34078720 3538957 (CObList))) FEATURES Location/Qualifiers misc_recomb 13506..17317 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(18416..19637) /vntifkey="22" /label=AmpR misc_marker 12055..13480 /vntifkey="22" /label=AmpR misc_recomb 8414..12059 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4460..8413 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 13487..13504 /vntifkey="21" /label=I-Sce\I misc_feature 3342..3375 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 3376..3386 /vntifkey="21" /label=Kozak\consensus misc_feature 3383..4102 /vntifkey="21" /label=EGFP misc_feature 4103..4138 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4139..4211 /vntifkey="21" /label=NLS misc_feature 4217..4250 /vntifkey="21" /label=MFRT misc_feature 4404..4454 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..3333 /vntifkey="21" /label=Ple150\(OLIG1-C) /note="1306" misc_feature 2863..3333 /vntifkey="21" /label=Region\Prom misc_feature 1200..1951 /vntifkey="21" /label=Region\6 misc_feature 1952..2862 /vntifkey="21" /label=Region\7 misc_feature 139..1199 /vntifkey="21" /label=Region\5 primer 2961..2980 /vntifkey="27" /label=oEMS2674 primer 2327..2346 /vntifkey="27" /label=oEMS2682 BASE COUNT 5325 a 4410 c 4434 g 5474 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccaa tctgatcctg tgaaaagtgt agcatcaaac ttcattttcc 181 ttccagaaaa tgctttttaa aaacctgcca aatcttggag gctggccttt tattggttct 241 ggggtgtggg gcaaacaagg gattttctga aataatttat cagttactat caacaaattt 301 gctaaagctt gacatataac attcgccact gtgaacaaaa gctgcctccc aaagtccctt 361 gggtagtctt tgcgcacact aaaaatccag agcttgaggc ataagactga ccttaacatt 421 aaatgaattc atggttggac actttgttct aatcctttgt gaagcaaact ccttggattt 481 ttaaaacatg ctatcggatg aatctttcca atgaggagca tataaatatc tcctttagta 541 gttcctacaa agcatccatg tcaatgtcat taacagaatg ggaatgatat tggagggagt 601 gggaggtggt tgcaacttgc aattcagaca gccagtgaaa accttaacat taagtatcct 661 taaaaaaaaa aaaaccctca gtggttgtat ttatcctgaa tgcaaaactg tcccctttgt 721 tgagattggc tgaactaact cagcctggag aacaatgttg aggctgctgg cgggccatgt 781 gctaatgtgc tcatgcatta caggccgcaa cagatggctg tgagaacacc cggcctgccc 841 cgcctgggtc aggatgcaga gcccaacgcc tgtttccagt gctgtcccgc tatgaaaaca 901 ctgcttctct ggggagaggc gatggctggc ctgggggagg ccagccatgt ctgttctacc 961 caattcaagg gcaaaggtgc aagggaataa tgataagggg tttatttgag ccatgttaca 1021 gaattgaaaa gtattttcat acaattgaaa gtcagaaaat gggagacact tgctatattg 1081 ttctatcatg ttagctcaca ggcctcattc atacatttaa tctttttttg ttaagagaaa 1141 gaagccacac ctcatgtttt tattttttta acttttattt tagactcgga tacaggtgca 1201 acagcaggta agaaaagcat gactcttacc aggagagtgg atggcatcca atcttcttca 1261 aatcgaggca cgaagtgttt agaagtacat agaatttgag caatctaaca tgtcatggaa 1321 caaacccaaa ccataacctt catgctaatg aaatcacaag aaataaatgc atgggagatt 1381 tgcatgaagg tgactgaaat taataaacta tatcgtgctc cattggaaag gatcgaagag 1441 cagatgctaa cttagctgac ctgacaataa gtggagtagt gattagccat gctgcagtga 1501 ctcactgacc tggcaagcac catctgagag cagtccaatt taagtcagtc accatagcgt 1561 cagggcggga gggagacagg gatctcccaa tgaggttcgt gcccttctct ctggaaagac 1621 cagctaaaga gtcaacaggg tagctgataa caccaaataa tgttgttcta cacataggag 1681 acaacagatg tgtcctggat gatttagaag aggggaagag aaagaacaca cgaggggccc 1741 agaagccagg gaaggcttca ggaaggaggg agatttcaac tggtcctgat agatgaatca 1801 gattgaagca gggaattgag ggatgctaac agttagacca cccttgggtt gattctgctc 1861 agagagttct agaatcctga tgatggaaag agcaaagtca actcatggta tggggtgaga 1921 cctgtgctcc atcgtcatag cccacaccat aatcaaccca ctgatattta ccgatagcct 1981 tccctcaccc ccacccccaa ccccactccc actgtgggac ttaaaaggac aaaatatttt 2041 tgtgaaaaca gcatttgtgt gctttttttt ttaagggcta agaatgtgag atctaaatca 2101 attctaatat atttcaaata tattcattat catgcactaa aagagaattc ccattttgtg 2161 gttctccaag gagagtgaca gagccttgtt tgtaacagcc ccgggcataa gcaatcattg 2221 tgtagcttaa ttagatattc cccgtgctag tgtgagggac atatgtccat tcactctgac 2281 aaacactgac agcggccggc gctttcatga tcctgctcta tgcagataaa ggcaacgcaa 2341 gtgcgagctc tcccaaggac ctgtggcccc agccggcccc ggcacaatca gcccgcattc 2401 accgattggt tccagtttat taatagccta tgaacttctc atctgctccc ggactggtta 2461 ggactgtcac ctgcaactgc caggccagaa aaggcttttc cactcctcgg gatccgagcg 2521 gatctgggtc tgctgggcca ggcgcacttt gtattttcca acacaaagaa caatggattt 2581 gaggagttgt aaattgcaag agtggaaaag cagatcacgt tttaggtggg tccagaaggg 2641 aaggccaccc cacagtcaaa gggaaggcaa ctagaagcta aaataggctt ggctaatttt 2701 aaaagctact taaattctta tagttccaat ttttaaatat accttcaaat gaataggagt 2761 ggaggggagc gtccaaatgg ggaaaagtct tggcccatgt cctcaccttc tctttggaaa 2821 aggcaaacta tgaaaacagg ggatgaatac cagaatgtcc ctacataaga cggtaaattt 2881 tatgttatga atatgtgatc acagttaaaa aaaaatatga gggaggttgt ttttgagtag 2941 acagttgctg gaggaaggca cgtgagttgc agcctgacca gtgcgaccag tcctccttca 3001 acagccccgc ttgccttgaa aaggcgccct tactccgctc agggggcctg tcccgcgcgg 3061 ctggcacagg cgctcacaat aacctcctaa agcggtgcca gccgcacctc cgcgcggccc 3121 cggcacaagc agccaatgaa cacgcggctg cgcccggcct cgcgcctcca ttggctgcgc 3181 tccgccaccc gctgccccgc aggttcccaa gccgggttta aaggggtagg gcgcgggcca 3241 gggccccacc atcgtttccc cgcgcgcagg tccgcgggga ggggcggcct gccgaccggc 3301 ccaccccagg gcgttcctga agggcgtcct cggggcgcgc cgaagttcct attcttcaaa 3361 aggtatagga acttccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 3421 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 3481 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 3541 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 3601 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 3661 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 3721 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 3781 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 3841 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3901 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3961 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 4021 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 4081 catggacgag ctgtacaagt tatttctgaa ctgctgcccg ggctgctgca tggaaccgga 4141 tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtagatc caaaaaagaa 4201 gagaaaggta atataagaag ttcctattct ctagaaagta taggaacttc agcggccgcg 4261 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct 4321 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt 4381 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 4441 atttttttca ctgcgaattc tcgtaaaact cttcatggca gtagttatta ttctctctct 4501 ctctcttttt cttttttctt gagacaggat atttctctgt tgcccaggct ggagtgcagt 4561 ggcacagtct tggctcactg cagcctggac ctcctgggct caagccatcc tcccacctca 4621 gcctcccaag tagctggggc tacaggcaca tggccaccag gcccagataa tttttcattt 4681 ttgtagagac tgagtctcac catgttaccc aggtttatta ttctcatttt ttagatgaag 4741 agactgaggt ccagagaagc tcaatgactt gcctagtttt acaaatctcc tgccatcaca 4801 tacccctcag cgtccttaat aagagggagg ccaccaacta tgtgctgggc actgtggtgg 4861 atgctggagc tatagggttg agtatataag aaatggtgtt gctggagcaa ctgttgcttg 4921 cttacctgac ctatctgaga attaattagc aggggaacat atttttgttt tcagattcaa 4981 tataagaact tgtgtgggca aaaataaaga tcagtagtaa taacagtagt tcccatttgc 5041 tgactgtact gtcctaagtg catatatata tacatacaca cacgcatacc tatactcctc 5101 taatactcaa aatgatcctg tttatgtatt gttaatatgc tcattttatt tttaaatttt 5161 tatttatttt tatttttatt tatttttgag acggagtctc attctgtcgc ggaggctgaa 5221 gtgcagtggt gcgatctcag ctcagtgcga cctccgcctc ccgggttcaa gtgattctcc 5281 tgcctcagcc tccggactag ctgggattac aggcgcccgc ctccacgccc agctaatttt 5341 tgtattttta gtagagatgg ggtttcgcca tgttggccag gctggtctcg tactcctgac 5401 cttgagtgat ccacctgcct cggcctccca aagtgctggg attacaggca tgagccaccg 5461 cgccgggcta atatgctcat tttagtgagg caaaaataga ggctcagagt ctgatttgta 5521 caaaactaca gagcagttaa gtgtcctctc agatgtgtac cctgatctgg gtgactctag 5581 gactctaggt ctcaactgtt acaaccagtt aagggtttgg ggaagcactg ggccaagagt 5641 caggaaaatg gaagccacag gtagtgcaag gtcttgggaa tgggacgtct ggtccaagga 5701 ttcacgcgat gactggaacc cgaagagccg gggcccggtt tacggccgcc atgaagcaac 5761 gcgcgccggt aggtttggga atcagggagc cctctgaata ggagactgag ttgggaggga 5821 aaggggcttc gctgggggag cctcggcttc ttctgggaga aaattcccac ggctacctag 5881 tgagcctgca aactggtagg cgccggcgta ggcgcgcggg cggggccggg ggcggggcct 5941 gcggggcgtg gcggggcggg cagagggcgg ggcctgcttc tcctcagctt caggcggctg 6001 cgacgagccc tcaggcgaac ctctcggctt tcccgcgcgg cgccgcctct tgctgcgcct 6061 ccgcctcctc ctctgctccg ccaccggctt cctcctcctg agcagtcagc ccgcgcgccg 6121 gccggctccg ttatggcgac ccgcagccct ggcgtcgtgg tgagcagctc ggcctgccgg 6181 ccctggccgg ttcaggccca cgcggcaggt ggcggccggg ccctgaggcg cgggatcgat 6241 ccatgacacc cccccgcccc ccatatctca ctataattca ctgtcaaatg gcttttatga 6301 tttggagttt ggggatactt ttaaagacag ggtcttggta gctcctagat ggcttgggat 6361 tcacttagta acccttgctg gcctaaaatt caggaacttc tacctcatct tcccaagtac 6421 tgggttagta gggatgtcca gcatatttag cttgctttaa tgattttgct gtactttttt 6481 ctgtgtgtac aggtgcatgt atatttataa aactaacata atatatttta cccccaaagt 6541 accataaccc taattaatgc tattaaatgt tttgtatgct gggctctggg catactaata 6601 taaccaatat tatacaatgc aaaacaattt tggaatatct tctgtaaaat tatcttatta 6661 gtaagttgta accatctcag acttctaagt ttgagtttgt aaaggatcag ttttttttgg 6721 tgacctacac acacacacac acacacacac acacacacac acacaggggg gtggattctg 6781 agaatttaga gaatttagat ggttaatacc agtttttatt tttattctgt gtgatattta 6841 tttattaaga tttatttatt tattacatgt aggtacactg tatctgtctt cagacacacc 6901 agaagagggc atcagatctc gttacagatg gttgtgagtc accatgtggt tgctgggatt 6961 tgaactcagg acctctggaa gaacagtcag tgctcttaac cgttgagcca tctcaccagc 7021 cccgtgatat tttatttaac ttactagaat atactgtgtt aaaacatatt ttaggggatt 7081 ggggagatgg cccattctgt aaagtgtctg ccatgtcatc ttcaggacct gcgtttgtaa 7141 ccccagcatg gagaagacag agacagggag acccctgggc ttcctggcaa gctagactag 7201 caaatttggt taactaggtt caatgagaga ctcagtctca aaacataaga agtgaatttt 7261 ttagacccaa tttcaatctc tgaactccat acgtatgcac atgtacctgc atataaatat 7321 atatacacac ccataaacac atgcacacac aaaaaatggt tcaaaagttc ttaaaatgta 7381 taagagtaga aagtgtagag ccgggagtgg tggcgcacgc ctttaatccc agcacttggg 7441 agcagaggca ggtggatttc tgagttcgag gccagcctgg tctacagagt gagttccagg 7501 acagccagag ctatacagag aaatcctgcc tcaaaaaacc aaaaataaag tgtatatact 7561 aatctttcat gcctaaaata tttatttttg ccacattttt catacctctt taatattttt 7621 ctttttctct ttgtgcttca aaggaaattt tgtgtcatgt tttacttttc attcttgact 7681 ctattcagca gtaagacgca gcatggcttt cttagagaag tataatctat tacaaaacgg 7741 taacatttaa ctgctctaca taccctcttg tagctaaatt cctctgttaa actaagactt 7801 attatctaaa cgtaattaaa tgttgatatc ttataatgct ccactttgaa acagctgtct 7861 ttcttttgca gattagcgat gatgaaccag gttatgacct agatttgttt tgtataccta 7921 atcattatgc cgaggatttg gaaaaagtgt ttattcctca tggactgatt atggacaggt 7981 tagtaagacc tcgattgaag ttatttttag agggtgtgtg tgtggtgtgt gtgtgtgtgt 8041 gtgtgtgtgt gtgtgtgtgt gtgtaagaga gagaggagag gcatgtgtga tatatatgtg 8101 aagtgtattg catgtacatg gaatccagag aaggacatta ggtgtcctac tctgttgctc 8161 tcttattccc ttgaatcagt gtctcttgct gaacctgggg catacatact gatttggcta 8221 ggctgcctgg ctagcaagac ccagggatct gcttgtctct gccccccagt gctgacgtta 8281 caagcaggtg caaccacatc tggtttttat atgggtactg ggggatctga acttagggcc 8341 tgatgcgtgc ccaagtgctc tcacccgcca agccacctct ccagccaaag ggtttttttt 8401 cttgtattaa ttctaactta gcaactgttg agtagttgat gtttgaatat aagctaagcc 8461 aacttattag aataaatgtt ttctattttt tacttgtatg tgtgagtgcc tgcatgtatg 8521 tatgtgaagc atatgtattc caggatgtca gaattgtgat ggattccctg gaactggagt 8581 tacagaccat tgtgaaccac tgtgtatggg tgctgggaac tgaactttag gtcctctgca 8641 aaaacagtaa ggatcttaac tgataaacta tctttctagc cctaaggctt tctactttta 8701 aagttcgttt ctctttctct ctctctctct ctctctctct ctctctctcc ctctccctcc 8761 ctcctcctcc tcctcctctt tcccccctct cgcctcctct ttctctctcc cctccctctc 8821 tctctctcac acacacacac agacagagag aggagagaga aaggaagcct tactaattaa 8881 ttacctagtt ttagacataa ctagtgggaa tccatagtaa gtaaatcaac attcttgaag 8941 agaggttaga agaattggct ctcgttgtcc atcgaggctg tataagatta ttcttctgtt 9001 ttctcagctt accatacata ttttctacgt agacaatacc aattcaaata cactgtacgt 9061 aaagtatatg cgcacacaca cacacacaca cacacacaca cacacacaag ttacatattg 9121 tcatatagta ctaaaattta tggacttttt catattaata catctagagc tatcatgttt 9181 tttatctttt aaaaattact ttacggggct acagagatgt ctcagcagtt aggagcactg 9241 actgctcttc cagaggtcct gagttcaatt cccagcaacc acatggtggc tcacaaccat 9301 ctgtaatggg atctgatcca ctcttatggt gtgtctgaag acagtgacag tgtactcata 9361 tacatgaaat aaatctttaa aaaattactt taccatcagg gtatatatat atatatatat 9421 gtgtgtgtgt gtgtgtgtgt gtatgaagta tttgctattt gagagggtct cactgtttag 9481 ctcagtctgg cttggaacgt tctgtatagc ccagactggc ctcaactcag cagtccccct 9541 gaaaggctct catatgtgga gattacaggt atgtgctact gtgcccagcc ctacatgtta 9601 ctttgttttt gaaatagggt atcaaatagc ttagactggc ttctaagtca gtgtgtagct 9661 aaggatggtc tgagcttctg aacctgctac ttcaactcct ggtgtgccat catggccagt 9721 ttgcacagtt gtgagagtag aactcaggac ctcctatgtg ttactagaca tgttctctac 9781 cagctgagct acatcccaat tcgcctacat ttacttttat tttttaaaag atttatttat 9841 gtatacagca ctctgcctgc atgtacatct gcatgccaga agagggcacc agatctcatc 9901 atacatagtt gtgagccacc atgtggttgc tggggattga actcagggcc tctggaagag 9961 cagtcagtgc tcttaacctc tgagccatct ctgtagcccc ctacatttat tttaaagatt 10021 ataggctatt ctattcatag atgtgccata actgatttaa ccagatcttc attattttgt 10081 acttagtaga gtatattaga gggaatgaat cttaaataca cattttgaca cttgctcagc 10141 tttagacaag taaaaatcac tggtcaagga aatggtgctt agtggatgct tctgaactgt 10201 caaattgtcc tccaacaagt tacatcattt tatacttcca acgttatgtg agagttcatt 10261 tttctttagg ctcttctagt attttatatt tgagaaataa aatttaacca aaatttgcca 10321 atctagtaag cttgttttta tttgtatctt taaattttat gatagatgtt atagtgtact 10381 ctcctctccc ctcccctccc ctctcctcct cagcaggatc ttgttatgta gccctaaggg 10441 atctaatact tactctgtag tccaggctgt ccttcagctc atgagacaca ccatcatgct 10501 tggcatgttt cttgttctga aaggtatctg gttttaattt tggttgagct ggtgtgttgg 10561 tgtatatctg taatctcagc acaggcaagt agagacaagg attatgatta cagtccagcc 10621 tgggctattt agagggattc tctttaatct ttcctcatgc cccaaaatct tacctttggt 10681 atatgaaaaa tagtctccac ttctgcaaaa tattgcttta tgaagtaaga attaattccc 10741 ttcatagaga caaggaatgt gtcctgtaaa agtttaatgt gtaagaagta tttgttataa 10801 aagataaata ttcagaatct tctttttaat tcctgatttt atttctatag gactgaaaga 10861 cttgctcgag atgtcatgaa ggagatggga ggccatcaca ttgtggccct ctgtgtgctc 10921 aaggggggct ataagttctt tgctgacctg ctggattaca ttaaagcact gaatagaaat 10981 agtgatagat ccattcctat gactgtagat tttatcagac tgaagagcta ctgtgtaagt 11041 ataattaact tataattaaa aaaatagggc cattctagtt ttatctatat tttttttaaa 11101 cttgtgcaaa ctatgctaca taatcaactt acttgattat gtaatatata tagtaatagt 11161 tggaattact attattacat atttattttt ctgtgctggg gtagaaccca gggaactaag 11221 tactgtaata accgagcttc attcatccta gtcattttta attttttttt ttttttttgt 11281 tttttcgaga caaggtttct ctgtgtagcc ctggctgtcc tggaactcac tctgtagacc 11341 aggctggcct cgaactcaga aatctgcctg cctctgcctc ccagtgctga gattaatgac 11401 gtgcaccacc actgcctggc atttttttaa agttttaatt tgagaccctt tctcactaac 11461 ttgctcacac tatcctcgaa ctcattacgt gggctggtct agaaatttca gtcaacttgc 11521 ctcagcttct caagtagctg ggattacagg caggtgctac cacttctggc catgatcatt 11581 gttgttgttg ctattattat tattattatt tcactgatac ttcatatcac aacacagaat 11641 agtaactgta gttcatgacc aacaaaaaag ttgtacagtt tctctgttgt cttattttga 11701 tctggatttt gatttgtttc atggcactgg gcaggctaat ctcaaactgc atggctcaaa 11761 tagtccttct gtcttaatct cccatgttct ggaagtatag gtatcactac acctagctgt 11821 attttcatat tttaaaaaaa acgcctaaga attgctattg aatgaggcgg agagatggct 11881 cagcagtcac aagtacttgt agaggactgg ggtttggtta ctagcacctc aaagatggct 11941 tacaattatc tgtagctcca attccagagg atctgtgtgg taaagctgca tgtatatggg 12001 catgcaggta aaactgctaa acatataaaa atagctcagt cttaagagaa ttggtcgact 12061 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 12121 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 12181 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 12241 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 12301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 12361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 12421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 12481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 12541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 12601 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 12661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 12721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 12781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 12841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 12901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 12961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 13021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 13081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 13141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 13201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 13261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 13321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 13381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 13441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag gtcgagtagg gataacaggg 13501 taatggatcc tcgttgggca cctgcactca tgtacgcagg cccacatata gatgtacaca 13561 cgcatatcat tcaaaataga aattatttat acaagtagtg cgttggagag ctggctcagg 13621 agttgttaat gcttgctacc cttgcaaagg acccaagttc aatttccagc acctgtgttg 13681 ggcatctcac aaccacatgt aaatctagtt tcaggggatt tcacccctct tctgaactct 13741 tcaggcactt atacccacaa atggacatct ccacatagag acacatactt ttaaaaaagt 13801 aatctagggt ctagagaaat ggttatagac cacggactgg gatcagttcc cagaagccac 13861 atcctgtggc tcacaattgc ctgtgaatca agttctagat ctggtggcct cgtctagcct 13921 gcagtcatca acaaacacat gcacacatcc atacacacat aatttaaaag aaagcgaaag 13981 taacctataa tgctaaacaa agcacattct cagtgtagac tgcagtggtg atgaaccttg 14041 aaaatatatt aatagaagcc agacacgtaa gtcacaaatg gtataactca atttatatga 14101 aattcccaga gagtccaaag ttaaaaaaaa caaaaaggca gattattggt tgtcagtagc 14161 tacagagatg ggacattgaa gatattaata ggtatagggt tgaggattca tggcctttcc 14221 ctcttccatg ttagcatgtc tattgttgtt aaccttattc aactcatgtt agggcaatca 14281 tattgatgag acttaatgag tatagcttga cattcctaga agacagagtc tcacatcaga 14341 ctctctgatc ctctgactct tagaatcttt caacaccctc ttctgaaatg atctctgagc 14401 ctttggttgt gactgaatta tgagaaagaa ttttatgttg aatgaatttt acttcagcat 14461 ttttacaaaa ctgaagctgg ggatggcgtt tcatgcgtgc agtccctacc atttgcctat 14521 aagcctggag gaggcagaag tagaattagg cttttcaggg acatcttcag ctgcatcatg 14581 agcttgagga caacttgagc tacataagat tcccatcccc acccccacac acacacacac 14641 atctagtatg tacctagtat gcagtctttt catatgttta aaatgtagaa aggtatatgt 14701 gaagtctccc ttttccccac aacttagtcc ctccaaagta ttgcatcagt ttccttgtac 14761 atgattttat gcatatataa gatacactat ttctctctct ctctctctcc ttccctcctt 14821 ccctccaacc tctgggtatg agttgagggt tatgtatgtt tcaagttgta ataagaattg 14881 ccactcaaac atttcatccc attttaagtt aagtttgtct gattttccac tttagaaggg 14941 cagccatgat ggctcatgcc tataatccca tcctagcact tgtaagccta atacataaga 15001 ctggaggatt gtcatcagct tgaggctagc ctggtctacc taatgagttc cagccagcca 15061 agagtagaat ataagaccct gtctccaaaa aatgtaccaa aggggctgga ggggtaactc 15121 agtggttaag aacagtagct gctcttccag aggacctggg ttcaattccc agcacccaca 15181 tggcagcttg caactatctg taactgtcca gctttgggat atccgacacc attataccaa 15241 ttacataaaa tgaaaaaatt gtaaaatgtg ctaaaatgag tggggagggg gagcatggtg 15301 tggttctgtg ggcttgggat tttgccatga atccatgact atatctcagc aatatgtgga 15361 ttatttctgg aaacctgtgg ggcatgggtg tatgggaaga ggggagagag agagagcccg 15421 catccagcca gagttcctgt gctctgggca ggcggacgtg ggaggactgc tggacgattt 15481 ccacttggcc ctgggtgggc atctggctgt gtgaagccac tgaccccact cagcgggggg 15541 tagataaggg gcatttccca taccaggttc tcagactcca gcattccacg ctggatacag 15601 cagaagagct gagaacagaa taggggcccc agggagacac tctcgtccca gagataaaga 15661 aggcagaggg agagagattc ctacgcaggc gagaggcctt agtctgggcc ttgatgaaca 15721 gagacggtct atggttttag agctttattg tagaaaggca gggggaaaga gagaaggtag 15781 aaagagagag accagccatg gccaagagga gagaaggggg gaaaggagag agagaagaaa 15841 ggctagagag taagaaaggt taaagagaca gaagtaaaag cttagagtaa gagagagaga 15901 gagagcaaga gggagagtaa gaaaagtaaa agcttaagag agcgaggagg ggccagcccc 15961 tctaatagtg ggcttgttat cttgttgttg ctaggtaact gtgaagcgtc tagcctgaag 16021 gccagaagct tgggacattg tctatgtgac tgaaagccat gagcctctac tgtgggggct 16081 gtggaggtgg taacttggac aggagccagg ggttccagga gacatgaggg aatgccttct 16141 gtcccatgta ggtaggaatt accaccaccg gctttcaccg tagttcactg gagactgttt 16201 gtacatagcc cattgcccca cagaaaccag gcactcactg tttcatttgc ataagaatta 16261 gaaggcttta ttttggctgg gattggtgac ttgccccaga aattccagct tttgggaagc 16321 cgaatcctgg agaaccagga gttcaaggtc atcctcaggt acctcatttg ttcaagacca 16381 gcctgtgcta ccatgagacc ttgtccctac tcccctcaac cccaaaacac gctgttttca 16441 ttttataggt tgtactaacc atggaggatg gtcatttgcc tttctcttga tttcagaagc 16501 gtagcgcggg ctttgcacca tgatctagtg tttccagata ggcttgcctc tccacagcca 16561 ggcttatcct gcttcagtcc catgtttggc aagggcaaca tatgactgaa gaacttgcat 16621 agccttatgg tgtatcatga ctcttagccc cagtttataa ctgtctaaca gctggtggct 16681 tttgtgaaaa aggaaagcat catttgaact tttaactttt cagctgtttg ttttgacttt 16741 gcataaatac tgagactagc taggaaatga gcttttgttt catttgctaa aaatgatttt 16801 tttttcatga ggcaatttca agcaatttga atttagcaat ctctacttgt ctgaaaagaa 16861 gttattaatt aaaaaaaaac aaacaaacag gaacctatta tgctggctag tcacatgaaa 16921 gaatccaaat gaacacagtc aaggctgaat caacacatga aattattttc taattcccca 16981 aattacattt atgctaatta gtatgtagta aaacatttta attgtttcca atgttaaaca 17041 ttacagaaat aattttgatg ttttcctaat aacccagcct ttgatcatgt ttgcatttat 17101 ttgattgttg tcagtttatt aaataattaa gtaagagcaa caagataaaa tgacttcatg 17161 agagtccttt tctgtaactt agaacgtcag tagtcatagg aactgcggtc gttcccttac 17221 ctttcagaag taggctaaag agttgaacgc aaaggtggtt tttcgaatct tcctgatcct 17281 taagccccca ctaggtttat attctatatt tgatatcgga ttcgaacccc tcgagcttag 17341 gatccggaac ccttaatata actaagttat taggtccctc gaagaggttc actagtactg 17401 gccattgcgg ccgcttcgag gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg 17461 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 17521 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc 17581 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg 17641 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 17701 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 17761 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 17821 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 17881 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 17941 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 18001 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 18061 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 18121 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 18181 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 18241 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 18301 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 18361 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 18421 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 18481 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 18541 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 18601 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 18661 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 18721 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 18781 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 18841 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 18901 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 18961 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 19021 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 19081 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 19141 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 19201 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 19261 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 19321 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 19381 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 19441 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 19501 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 19561 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 19621 ccccgaaaag tgccacctga cgg //