LOCUS Ple94\(pEMS1162) 19497 bp DNA circular 11-FEB-2010 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2653 - GATCAGGTGTGGGGTTCTGTGAT Tm=56.4 Deg C Antisense primer: oEMS2619 - CCCAGGACTGATCTCTTTATTCCC Tm=56.1 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 629 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|545679522| COMMENT VNTDBDATE|545679522| COMMENT LSOWNER| COMMENT VNTNAME|Ple94 (pEMS1162)| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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Location/Qualifiers misc_recomb 13360..17171 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(18270..19491) /vntifkey="22" /label=AmpR misc_marker 11909..13334 /vntifkey="22" /label=AmpR misc_recomb 8268..11913 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4314..8267 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 13341..13358 /vntifkey="21" /label=I-Sce\I misc_feature 3196..3229 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 3230..3240 /vntifkey="21" /label=Kozak\consensus misc_feature 3237..3956 /vntifkey="21" /label=EGFP misc_feature 3957..3992 /vntifkey="21" /label=Tetracysteine\residue misc_feature 3993..4065 /vntifkey="21" /label=NLS misc_feature 4071..4104 /vntifkey="21" /label=MFRT misc_feature 4258..4308 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..3187 /vntifkey="21" /label=Ple94\(GPR88-C) /note="1306" primer 1426..1448 /vntifkey="27" /label=oEMS2653 primer 2031..2054 /vntifkey="27" /label=oEMS2619 misc_feature 803..1785 /vntifkey="21" /label=Region\4 misc_feature 1786..3187 /vntifkey="21" /label=Region\Prom misc_feature 139..802 /vntifkey="21" /label=Region\3 BASE COUNT 5315 a 4333 c 4313 g 5536 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccgc ttaactctaa tgcaggatga taattgtggc taattgccta 181 aaagcatatg aacttttttt cccatcagtg ttatatgtga gagaacacac acacacacac 241 acacacacac ccctctttaa tatgaatttg atgtcctcac ttaaattaca tgaaataatt 301 cctaaatgct tgaaaataca aaaccctcct catctagttc ctatctcaat cagacaaata 361 ttttaaagca gattcaaaaa atagactata ctaaacttct tactggaaaa aaagtcactt 421 ctggacagag accaagccaa agtaatttta gctcaaattg gttttcctgg aaaaactgga 481 aacgtaatcc tgaaaagaaa tgtcttggca gctttacaaa tcaacactca aaaactaaca 541 agagcagcct aaatatcctt cacttcaatg attctcagat accagcatgg aaagaatctt 601 gtttgacaaa aaagcttgtt aaaattcaga ttctggaacc cagcctgcag atctaaacaa 661 aacccgagtt gtttaagtag agaagatttg aagatcacag cttaattaac tcagaaaaga 721 tcaatgtaga aatttataaa ttaataggat agaaaaagga attgtgtttg ggaaactatg 781 tcctagccca ctccaataga gctcagaacc actgctgtat agtgtagtcc cctgatatca 841 ctaccatagt gagctgctgc tgctggagtg aagctatcga acctctgaag tctggtaggg 901 caggagaagg ctgaaaacca tcattacaag gtttcatata tctcaggcaa agaatttcag 961 gatgaaatac actatgttat tgcagcttca tgattctata aattcataat ttcaaaaagc 1021 tctaccacta tggtttcttg tgatggtgaa tgctcacgtg cagctgaatt actctcaaat 1081 ctggctataa aacagcataa agatgttgtt cttttggaaa atatagagtt gaaagaggcc 1141 catatcaaca taattctcag ttgtgtaata tctgagagac caagatctga agaaatacag 1201 ctggggttgg aaatgggatt ttagacagtc tcagtgttgt gctcagtgag gttgctctgt 1261 ggcttggcta acagcacaga ggggagaaag gcaacaggaa atgagcactt tttcactgtt 1321 gctacatacc cttcggtttc gcaaagtatg agagacagct gctacatcat gttttggtgg 1381 atcatgaaat tcagtaccag gaaagtccac actggatcct gtcaggatca ggtgtggggt 1441 tctgtgatgt gttttcaaca aacactctgt ttaaaaataa tctggtagca taaaccagga 1501 ggatgtggtt acatgaaggt tttaaagtat tttagaaaaa taaatacaat gctctgcggc 1561 atgatctact aatgtgagtt gtgtctcttg agaaagataa ttgaggttga aatcttgatc 1621 ccacatataa aggttcccaa gggacatatt cagtcagcac atgattaaat atttacaaca 1681 tgtcatgtac tctaatcaaa aagagagtct aggcccctta ccgttgacta gcttaccgct 1741 tggagagggg atactcaatg ccggagaagt cagaccacgg cagtgtccat tgtctagatg 1801 taccactgac atctgcttta aatcttttgc ccatttcatt tttaccctct aaccatttca 1861 ttactaaaac acaaaacaaa ctccggaaag gagtttgttg atgagaaaac actgaaagta 1921 cgatttaaat tgaggaatgg agattttctg tggataattg tctatgttcc actttaccaa 1981 agaaaaatga atttcaagta gtgcataaag taattctcgt taaaaaatgt gggaataaag 2041 agatcagtcc tgggtactgg gaatatattt atctcacaga aaaatcttgc ccacaaaatg 2101 catcagactg agatgaaata ctctagggta tattaaattt tattctaata ttgaagggag 2161 ggaaaaaaag gacggaaaga aaaatgaaac aggatgaaaa ataaggtgat tgggagagag 2221 aaaagacaga gaaacttgtt aacgacagga ctttctgagc aagatatttt tcttcctttt 2281 acaagttgga agctattgaa taattcatgc caagcgtgga aaggctgtct tttccctgga 2341 tgatgtataa ctaagcagga gagagcttcg agtgggttca ccacatcagc caccactcct 2401 gcttctgagc acagggtgct ctcctcttga gctcagcttc tgcttttgca gccaagcatt 2461 cttgctgctg ctgcctgcct gcccacccgc ctgggcttgc agcccgccac tttactttct 2521 ccagccctga taccagctga gaagtctccc tgcagctgct agttcctgcc caggaccatg 2581 tgtgtggatg ctgcttggga gaagcgggca cttgctcctg gcactgatcc cagctgagtt 2641 tctcctgttg atttctggac cactgatgct gttgctgagg aggtatttcc cggcatccct 2701 ccccctgaga caccggctaa ggaccagcct aaacgcaagg taaccttcac ctctttccca 2761 tgggattttc cccagctggg atatctctgc tttctcctgg aactaggtcc tccagctggc 2821 aacatatctc tctctctctc tctctctctc tctctctctc tctgtgtgtg tgtgtgtgtg 2881 ttgggggaga agaggagact gttttaggta ttgctctgtc ctagggcttc cccgatacct 2941 gctgaatcca atgcaaagtg gcctttcccc tggctctgga gacatttccg aggcaggcac 3001 ctgtcatttg ccacccccgt cctcctcctc gagctccctt tctccccctc ccccagccca 3061 ttattctgct tcagcctttt gtgtcagtgg cagagggctg aagggatgtc tttgcccttc 3121 tggcaggcag gacagtgtca ggatggaccg cgctgccaga agccgacgct agcgagggag 3181 gtgtgaaggc gcgccgaagt tcctattctt caaaaggtat aggaacttcc gccaccatgg 3241 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 3301 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 3361 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 3421 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 3481 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 3541 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 3601 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 3661 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 3721 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 3781 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 3841 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 3901 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagttatttc 3961 tgaactgctg cccgggctgc tgcatggaac cggatccaaa aaagaagaga aaggtagatc 4021 caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtaatataa gaagttccta 4081 ttctctagaa agtataggaa cttcagcggc cgcgactcta gatcataatc agccatacca 4141 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 4201 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 4261 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcga attctcgtaa 4321 aactcttcat ggcagtagtt attattctct ctctctctct ttttcttttt tcttgagaca 4381 ggatatttct ctgttgccca ggctggagtg cagtggcaca gtcttggctc actgcagcct 4441 ggacctcctg ggctcaagcc atcctcccac ctcagcctcc caagtagctg gggctacagg 4501 cacatggcca ccaggcccag ataatttttc atttttgtag agactgagtc tcaccatgtt 4561 acccaggttt attattctca ttttttagat gaagagactg aggtccagag aagctcaatg 4621 acttgcctag ttttacaaat ctcctgccat cacatacccc tcagcgtcct taataagagg 4681 gaggccacca actatgtgct gggcactgtg gtggatgctg gagctatagg gttgagtata 4741 taagaaatgg tgttgctgga gcaactgttg cttgcttacc tgacctatct gagaattaat 4801 tagcagggga acatattttt gttttcagat tcaatataag aacttgtgtg ggcaaaaata 4861 aagatcagta gtaataacag tagttcccat ttgctgactg tactgtccta agtgcatata 4921 tatatacata cacacacgca tacctatact cctctaatac tcaaaatgat cctgtttatg 4981 tattgttaat atgctcattt tatttttaaa tttttattta tttttatttt tatttatttt 5041 tgagacggag tctcattctg tcgcggaggc tgaagtgcag tggtgcgatc tcagctcagt 5101 gcgacctccg cctcccgggt tcaagtgatt ctcctgcctc agcctccgga ctagctggga 5161 ttacaggcgc ccgcctccac gcccagctaa tttttgtatt tttagtagag atggggtttc 5221 gccatgttgg ccaggctggt ctcgtactcc tgaccttgag tgatccacct gcctcggcct 5281 cccaaagtgc tgggattaca ggcatgagcc accgcgccgg gctaatatgc tcattttagt 5341 gaggcaaaaa tagaggctca gagtctgatt tgtacaaaac tacagagcag ttaagtgtcc 5401 tctcagatgt gtaccctgat ctgggtgact ctaggactct aggtctcaac tgttacaacc 5461 agttaagggt ttggggaagc actgggccaa gagtcaggaa aatggaagcc acaggtagtg 5521 caaggtcttg ggaatgggac gtctggtcca aggattcacg cgatgactgg aacccgaaga 5581 gccggggccc ggtttacggc cgccatgaag caacgcgcgc cggtaggttt gggaatcagg 5641 gagccctctg aataggagac tgagttggga gggaaagggg cttcgctggg ggagcctcgg 5701 cttcttctgg gagaaaattc ccacggctac ctagtgagcc tgcaaactgg taggcgccgg 5761 cgtaggcgcg cgggcggggc cgggggcggg gcctgcgggg cgtggcgggg cgggcagagg 5821 gcggggcctg cttctcctca gcttcaggcg gctgcgacga gccctcaggc gaacctctcg 5881 gctttcccgc gcggcgccgc ctcttgctgc gcctccgcct cctcctctgc tccgccaccg 5941 gcttcctcct cctgagcagt cagcccgcgc gccggccggc tccgttatgg cgacccgcag 6001 ccctggcgtc gtggtgagca gctcggcctg ccggccctgg ccggttcagg cccacgcggc 6061 aggtggcggc cgggccctga ggcgcgggat cgatccatga cacccccccg ccccccatat 6121 ctcactataa ttcactgtca aatggctttt atgatttgga gtttggggat acttttaaag 6181 acagggtctt ggtagctcct agatggcttg ggattcactt agtaaccctt gctggcctaa 6241 aattcaggaa cttctacctc atcttcccaa gtactgggtt agtagggatg tccagcatat 6301 ttagcttgct ttaatgattt tgctgtactt ttttctgtgt gtacaggtgc atgtatattt 6361 ataaaactaa cataatatat tttaccccca aagtaccata accctaatta atgctattaa 6421 atgttttgta tgctgggctc tgggcatact aatataacca atattataca atgcaaaaca 6481 attttggaat atcttctgta aaattatctt attagtaagt tgtaaccatc tcagacttct 6541 aagtttgagt ttgtaaagga tcagtttttt ttggtgacct acacacacac acacacacac 6601 acacacacac acacacacag gggggtggat tctgagaatt tagagaattt agatggttaa 6661 taccagtttt tatttttatt ctgtgtgata tttatttatt aagatttatt tatttattac 6721 atgtaggtac actgtatctg tcttcagaca caccagaaga gggcatcaga tctcgttaca 6781 gatggttgtg agtcaccatg tggttgctgg gatttgaact caggacctct ggaagaacag 6841 tcagtgctct taaccgttga gccatctcac cagccccgtg atattttatt taacttacta 6901 gaatatactg tgttaaaaca tattttaggg gattggggag atggcccatt ctgtaaagtg 6961 tctgccatgt catcttcagg acctgcgttt gtaaccccag catggagaag acagagacag 7021 ggagacccct gggcttcctg gcaagctaga ctagcaaatt tggttaacta ggttcaatga 7081 gagactcagt ctcaaaacat aagaagtgaa ttttttagac ccaatttcaa tctctgaact 7141 ccatacgtat gcacatgtac ctgcatataa atatatatac acacccataa acacatgcac 7201 acacaaaaaa tggttcaaaa gttcttaaaa tgtataagag tagaaagtgt agagccggga 7261 gtggtggcgc acgcctttaa tcccagcact tgggagcaga ggcaggtgga tttctgagtt 7321 cgaggccagc ctggtctaca gagtgagttc caggacagcc agagctatac agagaaatcc 7381 tgcctcaaaa aaccaaaaat aaagtgtata tactaatctt tcatgcctaa aatatttatt 7441 tttgccacat ttttcatacc tctttaatat ttttcttttt ctctttgtgc ttcaaaggaa 7501 attttgtgtc atgttttact tttcattctt gactctattc agcagtaaga cgcagcatgg 7561 ctttcttaga gaagtataat ctattacaaa acggtaacat ttaactgctc tacataccct 7621 cttgtagcta aattcctctg ttaaactaag acttattatc taaacgtaat taaatgttga 7681 tatcttataa tgctccactt tgaaacagct gtctttcttt tgcagattag cgatgatgaa 7741 ccaggttatg acctagattt gttttgtata cctaatcatt atgccgagga tttggaaaaa 7801 gtgtttattc ctcatggact gattatggac aggttagtaa gacctcgatt gaagttattt 7861 ttagagggtg tgtgtgtggt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtaa 7921 gagagagagg agaggcatgt gtgatatata tgtgaagtgt attgcatgta catggaatcc 7981 agagaaggac attaggtgtc ctactctgtt gctctcttat tcccttgaat cagtgtctct 8041 tgctgaacct ggggcataca tactgatttg gctaggctgc ctggctagca agacccaggg 8101 atctgcttgt ctctgccccc cagtgctgac gttacaagca ggtgcaacca catctggttt 8161 ttatatgggt actgggggat ctgaacttag ggcctgatgc gtgcccaagt gctctcaccc 8221 gccaagccac ctctccagcc aaagggtttt ttttcttgta ttaattctaa cttagcaact 8281 gttgagtagt tgatgtttga atataagcta agccaactta ttagaataaa tgttttctat 8341 tttttacttg tatgtgtgag tgcctgcatg tatgtatgtg aagcatatgt attccaggat 8401 gtcagaattg tgatggattc cctggaactg gagttacaga ccattgtgaa ccactgtgta 8461 tgggtgctgg gaactgaact ttaggtcctc tgcaaaaaca gtaaggatct taactgataa 8521 actatctttc tagccctaag gctttctact tttaaagttc gtttctcttt ctctctctct 8581 ctctctctct ctctctctct ctccctctcc ctccctcctc ctcctcctcc tctttccccc 8641 ctctcgcctc ctctttctct ctcccctccc tctctctctc tcacacacac acacagacag 8701 agagaggaga gagaaaggaa gccttactaa ttaattacct agttttagac ataactagtg 8761 ggaatccata gtaagtaaat caacattctt gaagagaggt tagaagaatt ggctctcgtt 8821 gtccatcgag gctgtataag attattcttc tgttttctca gcttaccata catattttct 8881 acgtagacaa taccaattca aatacactgt acgtaaagta tatgcgcaca cacacacaca 8941 cacacacaca cacacacaca caagttacat attgtcatat agtactaaaa tttatggact 9001 ttttcatatt aatacatcta gagctatcat gttttttatc ttttaaaaat tactttacgg 9061 ggctacagag atgtctcagc agttaggagc actgactgct cttccagagg tcctgagttc 9121 aattcccagc aaccacatgg tggctcacaa ccatctgtaa tgggatctga tccactctta 9181 tggtgtgtct gaagacagtg acagtgtact catatacatg aaataaatct ttaaaaaatt 9241 actttaccat cagggtatat atatatatat atatgtgtgt gtgtgtgtgt gtgtgtatga 9301 agtatttgct atttgagagg gtctcactgt ttagctcagt ctggcttgga acgttctgta 9361 tagcccagac tggcctcaac tcagcagtcc ccctgaaagg ctctcatatg tggagattac 9421 aggtatgtgc tactgtgccc agccctacat gttactttgt ttttgaaata gggtatcaaa 9481 tagcttagac tggcttctaa gtcagtgtgt agctaaggat ggtctgagct tctgaacctg 9541 ctacttcaac tcctggtgtg ccatcatggc cagtttgcac agttgtgaga gtagaactca 9601 ggacctccta tgtgttacta gacatgttct ctaccagctg agctacatcc caattcgcct 9661 acatttactt ttatttttta aaagatttat ttatgtatac agcactctgc ctgcatgtac 9721 atctgcatgc cagaagaggg caccagatct catcatacat agttgtgagc caccatgtgg 9781 ttgctgggga ttgaactcag ggcctctgga agagcagtca gtgctcttaa cctctgagcc 9841 atctctgtag ccccctacat ttattttaaa gattataggc tattctattc atagatgtgc 9901 cataactgat ttaaccagat cttcattatt ttgtacttag tagagtatat tagagggaat 9961 gaatcttaaa tacacatttt gacacttgct cagctttaga caagtaaaaa tcactggtca 10021 aggaaatggt gcttagtgga tgcttctgaa ctgtcaaatt gtcctccaac aagttacatc 10081 attttatact tccaacgtta tgtgagagtt catttttctt taggctcttc tagtatttta 10141 tatttgagaa ataaaattta accaaaattt gccaatctag taagcttgtt tttatttgta 10201 tctttaaatt ttatgataga tgttatagtg tactctcctc tcccctcccc tcccctctcc 10261 tcctcagcag gatcttgtta tgtagcccta agggatctaa tacttactct gtagtccagg 10321 ctgtccttca gctcatgaga cacaccatca tgcttggcat gtttcttgtt ctgaaaggta 10381 tctggtttta attttggttg agctggtgtg ttggtgtata tctgtaatct cagcacaggc 10441 aagtagagac aaggattatg attacagtcc agcctgggct atttagaggg attctcttta 10501 atctttcctc atgccccaaa atcttacctt tggtatatga aaaatagtct ccacttctgc 10561 aaaatattgc tttatgaagt aagaattaat tcccttcata gagacaagga atgtgtcctg 10621 taaaagttta atgtgtaaga agtatttgtt ataaaagata aatattcaga atcttctttt 10681 taattcctga ttttatttct ataggactga aagacttgct cgagatgtca tgaaggagat 10741 gggaggccat cacattgtgg ccctctgtgt gctcaagggg ggctataagt tctttgctga 10801 cctgctggat tacattaaag cactgaatag aaatagtgat agatccattc ctatgactgt 10861 agattttatc agactgaaga gctactgtgt aagtataatt aacttataat taaaaaaata 10921 gggccattct agttttatct atattttttt taaacttgtg caaactatgc tacataatca 10981 acttacttga ttatgtaata tatatagtaa tagttggaat tactattatt acatatttat 11041 ttttctgtgc tggggtagaa cccagggaac taagtactgt aataaccgag cttcattcat 11101 cctagtcatt tttaattttt tttttttttt ttgttttttc gagacaaggt ttctctgtgt 11161 agccctggct gtcctggaac tcactctgta gaccaggctg gcctcgaact cagaaatctg 11221 cctgcctctg cctcccagtg ctgagattaa tgacgtgcac caccactgcc tggcattttt 11281 ttaaagtttt aatttgagac cctttctcac taacttgctc acactatcct cgaactcatt 11341 acgtgggctg gtctagaaat ttcagtcaac ttgcctcagc ttctcaagta gctgggatta 11401 caggcaggtg ctaccacttc tggccatgat cattgttgtt gttgctatta ttattattat 11461 tatttcactg atacttcata tcacaacaca gaatagtaac tgtagttcat gaccaacaaa 11521 aaagttgtac agtttctctg ttgtcttatt ttgatctgga ttttgatttg tttcatggca 11581 ctgggcaggc taatctcaaa ctgcatggct caaatagtcc ttctgtctta atctcccatg 11641 ttctggaagt ataggtatca ctacacctag ctgtattttc atattttaaa aaaaacgcct 11701 aagaattgct attgaatgag gcggagagat ggctcagcag tcacaagtac ttgtagagga 11761 ctggggtttg gttactagca cctcaaagat ggcttacaat tatctgtagc tccaattcca 11821 gaggatctgt gtggtaaagc tgcatgtata tgggcatgca ggtaaaactg ctaaacatat 11881 aaaaatagct cagtcttaag agaattggtc gactttttcg ccctttgacg ttggagtcca 11941 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 12001 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 12061 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac 12121 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 12181 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 12241 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 12301 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 12361 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 12421 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 12481 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 12541 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 12601 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 12661 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 12721 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 12781 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 12841 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 12901 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 12961 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 13021 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 13081 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 13141 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 13201 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 13261 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 13321 accaccgcta ccaggtcgag tagggataac agggtaatgg atcctcgttg ggcacctgca 13381 ctcatgtacg caggcccaca tatagatgta cacacgcata tcattcaaaa tagaaattat 13441 ttatacaagt agtgcgttgg agagctggct caggagttgt taatgcttgc tacccttgca 13501 aaggacccaa gttcaatttc cagcacctgt gttgggcatc tcacaaccac atgtaaatct 13561 agtttcaggg gatttcaccc ctcttctgaa ctcttcaggc acttataccc acaaatggac 13621 atctccacat agagacacat acttttaaaa aagtaatcta gggtctagag aaatggttat 13681 agaccacgga ctgggatcag ttcccagaag ccacatcctg tggctcacaa ttgcctgtga 13741 atcaagttct agatctggtg gcctcgtcta gcctgcagtc atcaacaaac acatgcacac 13801 atccatacac acataattta aaagaaagcg aaagtaacct ataatgctaa acaaagcaca 13861 ttctcagtgt agactgcagt ggtgatgaac cttgaaaata tattaataga agccagacac 13921 gtaagtcaca aatggtataa ctcaatttat atgaaattcc cagagagtcc aaagttaaaa 13981 aaaacaaaaa ggcagattat tggttgtcag tagctacaga gatgggacat tgaagatatt 14041 aataggtata gggttgagga ttcatggcct ttccctcttc catgttagca tgtctattgt 14101 tgttaacctt attcaactca tgttagggca atcatattga tgagacttaa tgagtatagc 14161 ttgacattcc tagaagacag agtctcacat cagactctct gatcctctga ctcttagaat 14221 ctttcaacac cctcttctga aatgatctct gagcctttgg ttgtgactga attatgagaa 14281 agaattttat gttgaatgaa ttttacttca gcatttttac aaaactgaag ctggggatgg 14341 cgtttcatgc gtgcagtccc taccatttgc ctataagcct ggaggaggca gaagtagaat 14401 taggcttttc agggacatct tcagctgcat catgagcttg aggacaactt gagctacata 14461 agattcccat ccccaccccc acacacacac acacatctag tatgtaccta gtatgcagtc 14521 ttttcatatg tttaaaatgt agaaaggtat atgtgaagtc tcccttttcc ccacaactta 14581 gtccctccaa agtattgcat cagtttcctt gtacatgatt ttatgcatat ataagataca 14641 ctatttctct ctctctctct ctccttccct ccttccctcc aacctctggg tatgagttga 14701 gggttatgta tgtttcaagt tgtaataaga attgccactc aaacatttca tcccatttta 14761 agttaagttt gtctgatttt ccactttaga agggcagcca tgatggctca tgcctataat 14821 cccatcctag cacttgtaag cctaatacat aagactggag gattgtcatc agcttgaggc 14881 tagcctggtc tacctaatga gttccagcca gccaagagta gaatataaga ccctgtctcc 14941 aaaaaatgta ccaaaggggc tggaggggta actcagtggt taagaacagt agctgctctt 15001 ccagaggacc tgggttcaat tcccagcacc cacatggcag cttgcaacta tctgtaactg 15061 tccagctttg ggatatccga caccattata ccaattacat aaaatgaaaa aattgtaaaa 15121 tgtgctaaaa tgagtgggga gggggagcat ggtgtggttc tgtgggcttg ggattttgcc 15181 atgaatccat gactatatct cagcaatatg tggattattt ctggaaacct gtggggcatg 15241 ggtgtatggg aagaggggag agagagagag cccgcatcca gccagagttc ctgtgctctg 15301 ggcaggcgga cgtgggagga ctgctggacg atttccactt ggccctgggt gggcatctgg 15361 ctgtgtgaag ccactgaccc cactcagcgg ggggtagata aggggcattt cccataccag 15421 gttctcagac tccagcattc cacgctggat acagcagaag agctgagaac agaatagggg 15481 ccccagggag acactctcgt cccagagata aagaaggcag agggagagag attcctacgc 15541 aggcgagagg ccttagtctg ggccttgatg aacagagacg gtctatggtt ttagagcttt 15601 attgtagaaa ggcaggggga aagagagaag gtagaaagag agagaccagc catggccaag 15661 aggagagaag gggggaaagg agagagagaa gaaaggctag agagtaagaa aggttaaaga 15721 gacagaagta aaagcttaga gtaagagaga gagagagagc aagagggaga gtaagaaaag 15781 taaaagctta agagagcgag gaggggccag cccctctaat agtgggcttg ttatcttgtt 15841 gttgctaggt aactgtgaag cgtctagcct gaaggccaga agcttgggac attgtctatg 15901 tgactgaaag ccatgagcct ctactgtggg ggctgtggag gtggtaactt ggacaggagc 15961 caggggttcc aggagacatg agggaatgcc ttctgtccca tgtaggtagg aattaccacc 16021 accggctttc accgtagttc actggagact gtttgtacat agcccattgc cccacagaaa 16081 ccaggcactc actgtttcat ttgcataaga attagaaggc tttattttgg ctgggattgg 16141 tgacttgccc cagaaattcc agcttttggg aagccgaatc ctggagaacc aggagttcaa 16201 ggtcatcctc aggtacctca tttgttcaag accagcctgt gctaccatga gaccttgtcc 16261 ctactcccct caaccccaaa acacgctgtt ttcattttat aggttgtact aaccatggag 16321 gatggtcatt tgcctttctc ttgatttcag aagcgtagcg cgggctttgc accatgatct 16381 agtgtttcca gataggcttg cctctccaca gccaggctta tcctgcttca gtcccatgtt 16441 tggcaagggc aacatatgac tgaagaactt gcatagcctt atggtgtatc atgactctta 16501 gccccagttt ataactgtct aacagctggt ggcttttgtg aaaaaggaaa gcatcatttg 16561 aacttttaac ttttcagctg tttgttttga ctttgcataa atactgagac tagctaggaa 16621 atgagctttt gtttcatttg ctaaaaatga tttttttttc atgaggcaat ttcaagcaat 16681 ttgaatttag caatctctac ttgtctgaaa agaagttatt aattaaaaaa aaacaaacaa 16741 acaggaacct attatgctgg ctagtcacat gaaagaatcc aaatgaacac agtcaaggct 16801 gaatcaacac atgaaattat tttctaattc cccaaattac atttatgcta attagtatgt 16861 agtaaaacat tttaattgtt tccaatgtta aacattacag aaataatttt gatgttttcc 16921 taataaccca gcctttgatc atgtttgcat ttatttgatt gttgtcagtt tattaaataa 16981 ttaagtaaga gcaacaagat aaaatgactt catgagagtc cttttctgta acttagaacg 17041 tcagtagtca taggaactgc ggtcgttccc ttacctttca gaagtaggct aaagagttga 17101 acgcaaaggt ggtttttcga atcttcctga tccttaagcc cccactaggt ttatattcta 17161 tatttgatat cggattcgaa cccctcgagc ttaggatccg gaacccttaa tataactaag 17221 ttattaggtc cctcgaagag gttcactagt actggccatt gcggccgctt cgaggctgcc 17281 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 17341 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 17401 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg 17461 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 17521 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac 17581 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 17641 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 17701 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 17761 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 17821 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 17881 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 17941 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 18001 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 18061 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 18121 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 18181 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 18241 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 18301 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 18361 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 18421 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 18481 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 18541 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 18601 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 18661 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 18721 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 18781 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc 18841 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 18901 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 18961 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 19021 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 19081 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 19141 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 19201 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 19261 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 19321 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 19381 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 19441 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgg //