LOCUS Ple58\(pEMS1127) 20035 bp DNA circular 3-FEB-2010 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2953 - 5' CCGCAAAGAACATCACCCCT- 3' Tm=56.2 Deg C Antisense primer: oEMS2948 - 5' TTCCAGGCAGCAGAACATGC - 3 Tm=56.0 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 527 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|543241613| COMMENT VNTDBDATE|544969001| COMMENT LSOWNER| COMMENT VNTNAME|Ple58 (pEMS1127)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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1684625219 (CObList))) FEATURES Location/Qualifiers misc_recomb 13898..17709 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(18808..20029) /vntifkey="22" /label=AmpR misc_marker 12447..13872 /vntifkey="22" /label=AmpR misc_recomb 8806..12451 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4852..8805 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 3754..3764 /vntifkey="21" /label=Kozak\consensus misc_feature 3761..4480 /vntifkey="21" /label=EGFP misc_feature 4481..4516 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4517..4589 /vntifkey="21" /label=NLS misc_feature 4796..4846 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 3706..3753 /vntifkey="21" /label=FFRT\(F5\mutant) misc_feature 4595..4642 /vntifkey="21" /label=FFRT misc_feature 13879..13896 /vntifkey="21" /label=I-Sce-I misc_feature 139..3697 /vntifkey="21" /label=Ple58\(DDC-C) /note="1307" misc_feature 3670..3697 /vntifkey="21" /label=Region\2 misc_feature 2291..3669 /vntifkey="21" /label=Prom\Region misc_feature 139..2290 /vntifkey="21" /label=Region\3 primer 1984..2003 /vntifkey="27" /label=oEMS2953 primer 2491..2510 /vntifkey="27" /label=oEMS2948 BASE COUNT 5276 a 4507 c 4659 g 5593 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggcctg ctaagaggaa gtaatttacg attttcttag gagtttattt 181 actagagtgt ctgtgggtga aattcagatt ccactgggca aatgaggaaa tgaaaaatgt 241 gggaatggat tcctttataa agaagtttgc ctgtgaagga aagaagagta aatagtgcca 301 ttgctggagc aagttgggcc gagggagatg tgctgagaga gagatttatc tgtgcatgtg 361 aaggagctgg tggaggaggc agaagcacga aagagaagac tacacatttc attaagagaa 421 acaaaatcaa attttatttt ctttgaggaa gatggaagga ccaaattaat atgcctctgt 481 tggaattttg ccctaaagac caaaattcca gaacattctg ctgatagaag ccacagatgt 541 gggccaattg taggtatcgg atcagagagg agaatcactt ttttgccgta gactgcccca 601 tgcaccggca ccgtaccagc cctggaggtg tgtggcgagc catggtccct ttcctcatgg 661 catggttctt ggtggggaag acaggtatgt tcccatctcc acgccatttg atgagaacaa 721 tgcatatata catgggtaga gggcgcaaat aaacttccta gaggggattt ggaaagcctg 781 ttgctgagag agaacacagg agctgtgtca ggggatatcc aggcaggtac ttctgggcat 841 ggtgcctttc ttgttggagg gaagagggct gcagcgtaca gagatcagaa aagcacagga 901 cattccagga aatgcacctt gtttgttgtc tttaactggc agccaaagga ttgtaaagct 961 ggagagagtc tgggccctca taggagaggg gttggagcct gagccttgtc tcactgccca 1021 gctgtgccct tgtggttata actgggcatg tgcataatga ctatatgcgt ccttctggga 1081 tgtagccaca tggtcttcat tatattctca aaggggctgt agatccctta aaattgaaga 1141 cttgctgctg agggcaaggg gcacatttgg cagggtttga accagaaaag ctgaatcatt 1201 gcccttccat gaacaggctt ggggcttgta ataaagtttc cccttgtttc tcaggacagc 1261 ttgcttccct aatgagccag cttgataagg tcgggccaca gagggcgatg cctgcgaggc 1321 acttacgtga gcacagaggg ctagtcgaag gggcaaggat gcagaagaga aggttgtgct 1381 gggctctgcc actgcaggaa gactgtgggc tctttgagat ggaggcctga actgtcccct 1441 acccctcctt ccccctgcac aaatgtgatt atgcctctgc agagcgcctg gaatcaggtt 1501 agcgtcattg agcaatgctg agctggagta atcagagcct ggagcggagc aggtgctggc 1561 tttaaagtgg cttctgagaa ttaggacagg ctgacttgta gctcagcccc acgtagacaa 1621 gccacttaga acactgtcct tgcagccccc aggcctcctc cttaccacag caaagtgctt 1681 gggtttgttg gaggggcctc tggcatggcc caggtgctga gctcgttaac tgatgttcag 1741 ggactggtgt cccaggggtc ctgtttgccc agcatgtggc aggacaacaa gagctaacca 1801 agcctggggt cacatttggt ctgctcctgc ctatctgggt gtcctgggac aattacccac 1861 ctcttggagc ctcacataag tcaactgtaa agtgggaatc acgagcatat ctaactcata 1921 aggtggtggc aattaaatat catataggaa aggctgtcag cattaggcct gctgcagagt 1981 tagccgcaaa gaacatcacc cctattctca acctaggcca ctgccaggac aaggccgggt 2041 tttattgttt ctgcaaagat gtttcatact cagaacaaaa aaaatcagta attacataag 2101 aatctaagaa taaaggttta tgttaaccaa aatcttaaga gaaatgcagt attactgttt 2161 ggtgaacatc actctagata ctcttctatg cacacattaa tagagagaaa ataattttaa 2221 taagggcaca tttaataaat ttttaataat agaagttatt ggctgggcat ggtggctctc 2281 cccagtaatc atggtgacgc ttgggcagct cccatcttgc cctctgtgca gatggggcct 2341 aatctcctta ctgcctccat tgtccacatg gctgctgcct gcaggtccct gcacggctgc 2401 tggagggagc tgcttatgca caggctgaga ccaggatccc ctcacttgta ccacctgcca 2461 ggtccccgcc ctgcagcccc tgcactcatg gcatgttctg ctgcctggaa ggccgctgcc 2521 tctgtcccag ttccatgggg atctctgctc tagaggggta gcttcttcct tgaaaagatg 2581 ctgcttaaag tcagaggatg gatggctctc cttggggcat ttctagctct ggactgtgcc 2641 acaagccctg gatgtggtat gtgtatgtgt gtgtatgcat gcacttaggt atgtgtgtgt 2701 gtgcatgtgt gtgtggtatg catgcatata tgcatgtgct attgtacgtg tgtagtgtgt 2761 ataaacatgc atgcatgtat gcttgtgtat gtgtgtgcat gcataagtgt gcatggtgtg 2821 tgtgcatgtg tgtgtggtcg gggcaggggc acctgcaggc tgctcgtcat ctgagtggac 2881 cctgttctcc actcttcctc ctctgcaaag cccaggcccc tttcctagct tcacatgact 2941 ctgcgtgcac cttcaggaga aacggagtcc tgctccttct attgcaccca tcaaccagga 3001 gtggggggag ggggtggagg tggggaagat gatcctccct gttgctgccc catggtggga 3061 ggagagactg agcccaaacc atgttttaga tgctgatagg cttaagggta acagcacagg 3121 agtttgagat gcatgcggct caacacctaa tctacatctc acttcacttt ctcatctggg 3181 gaagtgggct tgggaccctg agcctcccgg gtatcacagg gtcctaatag tccctcacag 3241 aaggagcaga cccagagtga gcactcccca aatgccacgc cgtcccttcc tcacccttgg 3301 agtggagcct ggtcgttctc aaagttgctg ggagagtccc aggagccctg gccccaaatc 3361 tgcatcctac acagtgcctg ggaacacagg gcccattttt tccttggcct ctcgccagtc 3421 ccagcaggcc ctgatgctcc tctccatcct gctaggatgg ctctctcccc ctgggggcag 3481 agtgggggca ggaggtggtg ggagggtgga gggatgctgc tcagtaaata atgcagagcc 3541 ggcagctctg attggcttcg gggaggcaga cactctgtct acataaatgg caatcacatc 3601 ttctgtgcct cttaactgtc actgtggaga ggagagagag aggacagaga gcaagtcact 3661 cccggctgct tttcacctct gacagagccc agacaccggc gcgccgaagt tcctattccg 3721 aagttcctat tcttcaaaag gtataggaac ttccgccacc atggtgagca agggcgagga 3781 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 3841 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 3901 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 3961 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 4021 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 4081 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 4141 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 4201 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 4261 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 4321 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 4381 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 4441 cgccgggatc actctcggca tggacgagct gtacaagtta tttctgaact gctgcccggg 4501 ctgctgcatg gaaccggatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa 4561 ggtagatcca aaaaagaaga gaaaggtaat ataagaagtt cctattccga agttcctatt 4621 ctctagaaag tataggaact tcagcggccg cgactctaga tcataatcag ccataccaca 4681 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 4741 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 4801 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcgaat tctcgtaaaa 4861 ctcttcatgg cagtagttat tattctctct ctctctcttt ttcttttttc ttgagacagg 4921 atatttctct gttgcccagg ctggagtgca gtggcacagt cttggctcac tgcagcctgg 4981 acctcctggg ctcaagccat cctcccacct cagcctccca agtagctggg gctacaggca 5041 catggccacc aggcccagat aatttttcat ttttgtagag actgagtctc accatgttac 5101 ccaggtttat tattctcatt ttttagatga agagactgag gtccagagaa gctcaatgac 5161 ttgcctagtt ttacaaatct cctgccatca catacccctc agcgtcctta ataagaggga 5221 ggccaccaac tatgtgctgg gcactgtggt ggatgctgga gctatagggt tgagtatata 5281 agaaatggtg ttgctggagc aactgttgct tgcttacctg acctatctga gaattaatta 5341 gcaggggaac atatttttgt tttcagattc aatataagaa cttgtgtggg caaaaataaa 5401 gatcagtagt aataacagta gttcccattt gctgactgta ctgtcctaag tgcatatata 5461 tatacataca cacacgcata cctatactcc tctaatactc aaaatgatcc tgtttatgta 5521 ttgttaatat gctcatttta tttttaaatt tttatttatt tttattttta tttatttttg 5581 agacggagtc tcattctgtc gcggaggctg aagtgcagtg gtgcgatctc agctcagtgc 5641 gacctccgcc tcccgggttc aagtgattct cctgcctcag cctccggact agctgggatt 5701 acaggcgccc gcctccacgc ccagctaatt tttgtatttt tagtagagat ggggtttcgc 5761 catgttggcc aggctggtct cgtactcctg accttgagtg atccacctgc ctcggcctcc 5821 caaagtgctg ggattacagg catgagccac cgcgccgggc taatatgctc attttagtga 5881 ggcaaaaata gaggctcaga gtctgatttg tacaaaacta cagagcagtt aagtgtcctc 5941 tcagatgtgt accctgatct gggtgactct aggactctag gtctcaactg ttacaaccag 6001 ttaagggttt ggggaagcac tgggccaaga gtcaggaaaa tggaagccac aggtagtgca 6061 aggtcttggg aatgggacgt ctggtccaag gattcacgcg atgactggaa cccgaagagc 6121 cggggcccgg tttacggccg ccatgaagca acgcgcgccg gtaggtttgg gaatcaggga 6181 gccctctgaa taggagactg agttgggagg gaaaggggct tcgctggggg agcctcggct 6241 tcttctggga gaaaattccc acggctacct agtgagcctg caaactggta ggcgccggcg 6301 taggcgcgcg ggcggggccg ggggcggggc ctgcggggcg tggcggggcg ggcagagggc 6361 ggggcctgct tctcctcagc ttcaggcggc tgcgacgagc cctcaggcga acctctcggc 6421 tttcccgcgc ggcgccgcct cttgctgcgc ctccgcctcc tcctctgctc cgccaccggc 6481 ttcctcctcc tgagcagtca gcccgcgcgc cggccggctc cgttatggcg acccgcagcc 6541 ctggcgtcgt ggtgagcagc tcggcctgcc ggccctggcc ggttcaggcc cacgcggcag 6601 gtggcggccg ggccctgagg cgcgggatcg atccatgaca cccccccgcc ccccatatct 6661 cactataatt cactgtcaaa tggcttttat gatttggagt ttggggatac ttttaaagac 6721 agggtcttgg tagctcctag atggcttggg attcacttag taacccttgc tggcctaaaa 6781 ttcaggaact tctacctcat cttcccaagt actgggttag tagggatgtc cagcatattt 6841 agcttgcttt aatgattttg ctgtactttt ttctgtgtgt acaggtgcat gtatatttat 6901 aaaactaaca taatatattt tacccccaaa gtaccataac cctaattaat gctattaaat 6961 gttttgtatg ctgggctctg ggcatactaa tataaccaat attatacaat gcaaaacaat 7021 tttggaatat cttctgtaaa attatcttat tagtaagttg taaccatctc agacttctaa 7081 gtttgagttt gtaaaggatc agtttttttt ggtgacctac acacacacac acacacacac 7141 acacacacac acacacaggg gggtggattc tgagaattta gagaatttag atggttaata 7201 ccagttttta tttttattct gtgtgatatt tatttattaa gatttattta tttattacat 7261 gtaggtacac tgtatctgtc ttcagacaca ccagaagagg gcatcagatc tcgttacaga 7321 tggttgtgag tcaccatgtg gttgctggga tttgaactca ggacctctgg aagaacagtc 7381 agtgctctta accgttgagc catctcacca gccccgtgat attttattta acttactaga 7441 atatactgtg ttaaaacata ttttagggga ttggggagat ggcccattct gtaaagtgtc 7501 tgccatgtca tcttcaggac ctgcgtttgt aaccccagca tggagaagac agagacaggg 7561 agacccctgg gcttcctggc aagctagact agcaaatttg gttaactagg ttcaatgaga 7621 gactcagtct caaaacataa gaagtgaatt ttttagaccc aatttcaatc tctgaactcc 7681 atacgtatgc acatgtacct gcatataaat atatatacac acccataaac acatgcacac 7741 acaaaaaatg gttcaaaagt tcttaaaatg tataagagta gaaagtgtag agccgggagt 7801 ggtggcgcac gcctttaatc ccagcacttg ggagcagagg caggtggatt tctgagttcg 7861 aggccagcct ggtctacaga gtgagttcca ggacagccag agctatacag agaaatcctg 7921 cctcaaaaaa ccaaaaataa agtgtatata ctaatctttc atgcctaaaa tatttatttt 7981 tgccacattt ttcatacctc tttaatattt ttctttttct ctttgtgctt caaaggaaat 8041 tttgtgtcat gttttacttt tcattcttga ctctattcag cagtaagacg cagcatggct 8101 ttcttagaga agtataatct attacaaaac ggtaacattt aactgctcta cataccctct 8161 tgtagctaaa ttcctctgtt aaactaagac ttattatcta aacgtaatta aatgttgata 8221 tcttataatg ctccactttg aaacagctgt ctttcttttg cagattagcg atgatgaacc 8281 aggttatgac ctagatttgt tttgtatacc taatcattat gccgaggatt tggaaaaagt 8341 gtttattcct catggactga ttatggacag gttagtaaga cctcgattga agttattttt 8401 agagggtgtg tgtgtggtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtaaga 8461 gagagaggag aggcatgtgt gatatatatg tgaagtgtat tgcatgtaca tggaatccag 8521 agaaggacat taggtgtcct actctgttgc tctcttattc ccttgaatca gtgtctcttg 8581 ctgaacctgg ggcatacata ctgatttggc taggctgcct ggctagcaag acccagggat 8641 ctgcttgtct ctgcccccca gtgctgacgt tacaagcagg tgcaaccaca tctggttttt 8701 atatgggtac tgggggatct gaacttaggg cctgatgcgt gcccaagtgc tctcacccgc 8761 caagccacct ctccagccaa agggtttttt ttcttgtatt aattctaact tagcaactgt 8821 tgagtagttg atgtttgaat ataagctaag ccaacttatt agaataaatg ttttctattt 8881 tttacttgta tgtgtgagtg cctgcatgta tgtatgtgaa gcatatgtat tccaggatgt 8941 cagaattgtg atggattccc tggaactgga gttacagacc attgtgaacc actgtgtatg 9001 ggtgctggga actgaacttt aggtcctctg caaaaacagt aaggatctta actgataaac 9061 tatctttcta gccctaaggc tttctacttt taaagttcgt ttctctttct ctctctctct 9121 ctctctctct ctctctctct ccctctccct ccctcctcct cctcctcctc tttcccccct 9181 ctcgcctcct ctttctctct cccctccctc tctctctctc acacacacac acagacagag 9241 agaggagaga gaaaggaagc cttactaatt aattacctag ttttagacat aactagtggg 9301 aatccatagt aagtaaatca acattcttga agagaggtta gaagaattgg ctctcgttgt 9361 ccatcgaggc tgtataagat tattcttctg ttttctcagc ttaccataca tattttctac 9421 gtagacaata ccaattcaaa tacactgtac gtaaagtata tgcgcacaca cacacacaca 9481 cacacacaca cacacacaca agttacatat tgtcatatag tactaaaatt tatggacttt 9541 ttcatattaa tacatctaga gctatcatgt tttttatctt ttaaaaatta ctttacgggg 9601 ctacagagat gtctcagcag ttaggagcac tgactgctct tccagaggtc ctgagttcaa 9661 ttcccagcaa ccacatggtg gctcacaacc atctgtaatg ggatctgatc cactcttatg 9721 gtgtgtctga agacagtgac agtgtactca tatacatgaa ataaatcttt aaaaaattac 9781 tttaccatca gggtatatat atatatatat atgtgtgtgt gtgtgtgtgt gtgtatgaag 9841 tatttgctat ttgagagggt ctcactgttt agctcagtct ggcttggaac gttctgtata 9901 gcccagactg gcctcaactc agcagtcccc ctgaaaggct ctcatatgtg gagattacag 9961 gtatgtgcta ctgtgcccag ccctacatgt tactttgttt ttgaaatagg gtatcaaata 10021 gcttagactg gcttctaagt cagtgtgtag ctaaggatgg tctgagcttc tgaacctgct 10081 acttcaactc ctggtgtgcc atcatggcca gtttgcacag ttgtgagagt agaactcagg 10141 acctcctatg tgttactaga catgttctct accagctgag ctacatccca attcgcctac 10201 atttactttt attttttaaa agatttattt atgtatacag cactctgcct gcatgtacat 10261 ctgcatgcca gaagagggca ccagatctca tcatacatag ttgtgagcca ccatgtggtt 10321 gctggggatt gaactcaggg cctctggaag agcagtcagt gctcttaacc tctgagccat 10381 ctctgtagcc ccctacattt attttaaaga ttataggcta ttctattcat agatgtgcca 10441 taactgattt aaccagatct tcattatttt gtacttagta gagtatatta gagggaatga 10501 atcttaaata cacattttga cacttgctca gctttagaca agtaaaaatc actggtcaag 10561 gaaatggtgc ttagtggatg cttctgaact gtcaaattgt cctccaacaa gttacatcat 10621 tttatacttc caacgttatg tgagagttca tttttcttta ggctcttcta gtattttata 10681 tttgagaaat aaaatttaac caaaatttgc caatctagta agcttgtttt tatttgtatc 10741 tttaaatttt atgatagatg ttatagtgta ctctcctctc ccctcccctc ccctctcctc 10801 ctcagcagga tcttgttatg tagccctaag ggatctaata cttactctgt agtccaggct 10861 gtccttcagc tcatgagaca caccatcatg cttggcatgt ttcttgttct gaaaggtatc 10921 tggttttaat tttggttgag ctggtgtgtt ggtgtatatc tgtaatctca gcacaggcaa 10981 gtagagacaa ggattatgat tacagtccag cctgggctat ttagagggat tctctttaat 11041 ctttcctcat gccccaaaat cttacctttg gtatatgaaa aatagtctcc acttctgcaa 11101 aatattgctt tatgaagtaa gaattaattc ccttcataga gacaaggaat gtgtcctgta 11161 aaagtttaat gtgtaagaag tatttgttat aaaagataaa tattcagaat cttcttttta 11221 attcctgatt ttatttctat aggactgaaa gacttgctcg agatgtcatg aaggagatgg 11281 gaggccatca cattgtggcc ctctgtgtgc tcaagggggg ctataagttc tttgctgacc 11341 tgctggatta cattaaagca ctgaatagaa atagtgatag atccattcct atgactgtag 11401 attttatcag actgaagagc tactgtgtaa gtataattaa cttataatta aaaaaatagg 11461 gccattctag ttttatctat atttttttta aacttgtgca aactatgcta cataatcaac 11521 ttacttgatt atgtaatata tatagtaata gttggaatta ctattattac atatttattt 11581 ttctgtgctg gggtagaacc cagggaacta agtactgtaa taaccgagct tcattcatcc 11641 tagtcatttt taattttttt tttttttttt gttttttcga gacaaggttt ctctgtgtag 11701 ccctggctgt cctggaactc actctgtaga ccaggctggc ctcgaactca gaaatctgcc 11761 tgcctctgcc tcccagtgct gagattaatg acgtgcacca ccactgcctg gcattttttt 11821 aaagttttaa tttgagaccc tttctcacta acttgctcac actatcctcg aactcattac 11881 gtgggctggt ctagaaattt cagtcaactt gcctcagctt ctcaagtagc tgggattaca 11941 ggcaggtgct accacttctg gccatgatca ttgttgttgt tgctattatt attattatta 12001 tttcactgat acttcatatc acaacacaga atagtaactg tagttcatga ccaacaaaaa 12061 agttgtacag tttctctgtt gtcttatttt gatctggatt ttgatttgtt tcatggcact 12121 gggcaggcta atctcaaact gcatggctca aatagtcctt ctgtcttaat ctcccatgtt 12181 ctggaagtat aggtatcact acacctagct gtattttcat attttaaaaa aaacgcctaa 12241 gaattgctat tgaatgaggc ggagagatgg ctcagcagtc acaagtactt gtagaggact 12301 ggggtttggt tactagcacc tcaaagatgg cttacaatta tctgtagctc caattccaga 12361 ggatctgtgt ggtaaagctg catgtatatg ggcatgcagg taaaactgct aaacatataa 12421 aaatagctca gtcttaagag aattggtcga ctttttcgcc ctttgacgtt ggagtccacg 12481 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 12541 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 12601 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt 12661 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 12721 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 12781 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 12841 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 12901 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 12961 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 13021 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 13081 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 13141 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 13201 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 13261 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 13321 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 13381 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 13441 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 13501 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 13561 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 13621 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 13681 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 13741 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 13801 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 13861 caccgctacc aggtcgagta gggataacag ggtaatggat cctcgttggg cacctgcact 13921 catgtacgca ggcccacata tagatgtaca cacgcatatc attcaaaata gaaattattt 13981 atacaagtag tgcgttggag agctggctca ggagttgtta atgcttgcta cccttgcaaa 14041 ggacccaagt tcaatttcca gcacctgtgt tgggcatctc acaaccacat gtaaatctag 14101 tttcagggga tttcacccct cttctgaact cttcaggcac ttatacccac aaatggacat 14161 ctccacatag agacacatac ttttaaaaaa gtaatctagg gtctagagaa atggttatag 14221 accacggact gggatcagtt cccagaagcc acatcctgtg gctcacaatt gcctgtgaat 14281 caagttctag atctggtggc ctcgtctagc ctgcagtcat caacaaacac atgcacacat 14341 ccatacacac ataatttaaa agaaagcgaa agtaacctat aatgctaaac aaagcacatt 14401 ctcagtgtag actgcagtgg tgatgaacct tgaaaatata ttaatagaag ccagacacgt 14461 aagtcacaaa tggtataact caatttatat gaaattccca gagagtccaa agttaaaaaa 14521 aacaaaaagg cagattattg gttgtcagta gctacagaga tgggacattg aagatattaa 14581 taggtatagg gttgaggatt catggccttt ccctcttcca tgttagcatg tctattgttg 14641 ttaaccttat tcaactcatg ttagggcaat catattgatg agacttaatg agtatagctt 14701 gacattccta gaagacagag tctcacatca gactctctga tcctctgact cttagaatct 14761 ttcaacaccc tcttctgaaa tgatctctga gcctttggtt gtgactgaat tatgagaaag 14821 aattttatgt tgaatgaatt ttacttcagc atttttacaa aactgaagct ggggatggcg 14881 tttcatgcgt gcagtcccta ccatttgcct ataagcctgg aggaggcaga agtagaatta 14941 ggcttttcag ggacatcttc agctgcatca tgagcttgag gacaacttga gctacataag 15001 attcccatcc ccacccccac acacacacac acatctagta tgtacctagt atgcagtctt 15061 ttcatatgtt taaaatgtag aaaggtatat gtgaagtctc ccttttcccc acaacttagt 15121 ccctccaaag tattgcatca gtttccttgt acatgatttt atgcatatat aagatacact 15181 atttctctct ctctctctct ccttccctcc ttccctccaa cctctgggta tgagttgagg 15241 gttatgtatg tttcaagttg taataagaat tgccactcaa acatttcatc ccattttaag 15301 ttaagtttgt ctgattttcc actttagaag ggcagccatg atggctcatg cctataatcc 15361 catcctagca cttgtaagcc taatacataa gactggagga ttgtcatcag cttgaggcta 15421 gcctggtcta cctaatgagt tccagccagc caagagtaga atataagacc ctgtctccaa 15481 aaaatgtacc aaaggggctg gaggggtaac tcagtggtta agaacagtag ctgctcttcc 15541 agaggacctg ggttcaattc ccagcaccca catggcagct tgcaactatc tgtaactgtc 15601 cagctttggg atatccgaca ccattatacc aattacataa aatgaaaaaa ttgtaaaatg 15661 tgctaaaatg agtggggagg gggagcatgg tgtggttctg tgggcttggg attttgccat 15721 gaatccatga ctatatctca gcaatatgtg gattatttct ggaaacctgt ggggcatggg 15781 tgtatgggaa gaggggagag agagagagcc cgcatccagc cagagttcct gtgctctggg 15841 caggcggacg tgggaggact gctggacgat ttccacttgg ccctgggtgg gcatctggct 15901 gtgtgaagcc actgacccca ctcagcgggg ggtagataag gggcatttcc cataccaggt 15961 tctcagactc cagcattcca cgctggatac agcagaagag ctgagaacag aataggggcc 16021 ccagggagac actctcgtcc cagagataaa gaaggcagag ggagagagat tcctacgcag 16081 gcgagaggcc ttagtctggg ccttgatgaa cagagacggt ctatggtttt agagctttat 16141 tgtagaaagg cagggggaaa gagagaaggt agaaagagag agaccagcca tggccaagag 16201 gagagaaggg gggaaaggag agagagaaga aaggctagag agtaagaaag gttaaagaga 16261 cagaagtaaa agcttagagt aagagagaga gagagagcaa gagggagagt aagaaaagta 16321 aaagcttaag agagcgagga ggggccagcc cctctaatag tgggcttgtt atcttgttgt 16381 tgctaggtaa ctgtgaagcg tctagcctga aggccagaag cttgggacat tgtctatgtg 16441 actgaaagcc atgagcctct actgtggggg ctgtggaggt ggtaacttgg acaggagcca 16501 ggggttccag gagacatgag ggaatgcctt ctgtcccatg taggtaggaa ttaccaccac 16561 cggctttcac cgtagttcac tggagactgt ttgtacatag cccattgccc cacagaaacc 16621 aggcactcac tgtttcattt gcataagaat tagaaggctt tattttggct gggattggtg 16681 acttgcccca gaaattccag cttttgggaa gccgaatcct ggagaaccag gagttcaagg 16741 tcatcctcag gtacctcatt tgttcaagac cagcctgtgc taccatgaga ccttgtccct 16801 actcccctca accccaaaac acgctgtttt cattttatag gttgtactaa ccatggagga 16861 tggtcatttg cctttctctt gatttcagaa gcgtagcgcg ggctttgcac catgatctag 16921 tgtttccaga taggcttgcc tctccacagc caggcttatc ctgcttcagt cccatgtttg 16981 gcaagggcaa catatgactg aagaacttgc atagccttat ggtgtatcat gactcttagc 17041 cccagtttat aactgtctaa cagctggtgg cttttgtgaa aaaggaaagc atcatttgaa 17101 cttttaactt ttcagctgtt tgttttgact ttgcataaat actgagacta gctaggaaat 17161 gagcttttgt ttcatttgct aaaaatgatt tttttttcat gaggcaattt caagcaattt 17221 gaatttagca atctctactt gtctgaaaag aagttattaa ttaaaaaaaa acaaacaaac 17281 aggaacctat tatgctggct agtcacatga aagaatccaa atgaacacag tcaaggctga 17341 atcaacacat gaaattattt tctaattccc caaattacat ttatgctaat tagtatgtag 17401 taaaacattt taattgtttc caatgttaaa cattacagaa ataattttga tgttttccta 17461 ataacccagc ctttgatcat gtttgcattt atttgattgt tgtcagttta ttaaataatt 17521 aagtaagagc aacaagataa aatgacttca tgagagtcct tttctgtaac ttagaacgtc 17581 agtagtcata ggaactgcgg tcgttccctt acctttcaga agtaggctaa agagttgaac 17641 gcaaaggtgg tttttcgaat cttcctgatc cttaagcccc cactaggttt atattctata 17701 tttgatatcg gattcgaacc cctcgagctt aggatccgga acccttaata taactaagtt 17761 attaggtccc tcgaagaggt tcactagtac tggccattgc ggccgcttcg aggctgcctc 17821 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 17881 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 17941 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc 18001 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 18061 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg 18121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 18181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 18241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 18301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 18361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 18421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 18481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 18541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 18601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 18661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 18721 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 18781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 18841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 18901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 18961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 19021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 19081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 19141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 19201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 19261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 19321 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt 19381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 19441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 19501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 19561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 19621 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata 19681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 19741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 19801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 19861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 19921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 19981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgg //