LOCUS Ple53\(pEMS1524) 22357 bp DNA circular 21-DEC-2009 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2364 - 5' - GCGTATCACGAGGCCCTTTC- 3' Tm=56.0 Deg C Antisense primer: oEMS2753 - 5' GCTGCCACCAAGTGGGATAAA- 3 Tm=56.3 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 735 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|536514927| COMMENT VNTDBDATE|538225422| COMMENT LSOWNER| COMMENT VNTNAME|Ple53 (pEMS1524)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#165=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #107# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.670712 5.24678 COMMENT 6.23446 1 0.082322) 0.8 1.8 0) COMMENT #166=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #103# 1768254544 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "LongProm Region" "@N" 1 13.8122 6.96932 0 COMMENT -10 8.51695 1.17389 #165#)) (CObjectList)) COMMENT #167=(CGroupWidget COMMENT (CWidget 22 (7 22 0) 1 2 0 0 Nil -116 100) COMMENT (CObjectList COMMENT #168=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #1# 0) 1 2 0 0 #119# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 COMMENT 0.00168355 0.344566 1 0.082322) 0.8 1.8 1) COMMENT #169=(CLabel (CWidget 0 (0 0) 1 2 0 0 #103# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -4.14436 12.1963 0 -10 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#175=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #111# 31125832 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 6.25831 1.9 COMMENT 0.082322 1) COMMENT #176=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #103# 2082617548 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "oEMS2364" "@N" COMMENT 1 3.21554 12.1963 0 -10 4.79547 1.17389 #175#)) COMMENT (CObjectList)) COMMENT #177=(CGroupWidget COMMENT (CWidget 86 (7 86 0) 1 2 0 0 Nil -395 100) COMMENT (CObjectList COMMENT #178=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #0# 0) 1 2 0 0 #107# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 0.652656 COMMENT 1.72227 1 0.082322) 0.8 1.8 0) COMMENT #179=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #103# 4063824 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "5' homology sequence" "@N" 1 -15.8883 COMMENT 5.22699 0 -10 10.4901 1.17389 #178#) COMMENT #180=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #3# 0) 1 2 0 0 #107# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.12801 3.15104 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #181=(CLabel (CWidget 0 (0 0) 1 2 0 0 #103# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "3' homology sequence" "@N" 1 -11.0558 COMMENT -10.454 0 -10 10.4901 1.17389 #180#) COMMENT #182=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #107# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 3.15104 COMMENT 4.2605 1 0.082322) 0.8 1.8 0) COMMENT #183=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #103# 21224012 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "HPRT complementary sequence" "@N" 1 13.5512 COMMENT -10.454 0 -10 14.9609 1.17389 #182#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #184=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -116 100) COMMENT (CObjectList) (CObjectList)) COMMENT #185=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -256 100) COMMENT (CObjectList) (CObjectList)) COMMENT #186=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -605 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) COMMENT (CObList #187=(AnlzFontColorItem 767 787 33554687) COMMENT #188=(AnlzFontColorItem 53 72 33554687)) 1 COMMENT (CObList #189=(AnlzFontBackColorItem 138 3657 33619967))) (CObList) 0 COMMENT (CStringList) 21972360 21902384 (CObList))) FEATURES Location/Qualifiers misc_recomb 16220..20031 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(21130..22351) /vntifkey="22" /label=AmpR misc_marker 14769..16194 /vntifkey="22" /label=AmpR misc_recomb 11128..14773 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 7174..11127 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 16201..16218 /vntifkey="21" /label=I-Sce\I misc_feature 3800..3847 /vntifkey="21" /label=FFRT\(F5\mutant) misc_feature 6918..6964 /vntifkey="21" /label=FFRT misc_feature 7118..7168 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 3848..3858 /vntifkey="21" /label=Kozak\consensus misc_feature 3667..3799 /vntifkey="21" /label=Intron misc_feature 3859..6917 /vntifkey="21" /label=lacZ misc_feature 139..3658 /vntifkey="21" /label=Ple53\(DCX-B) /note="1313" primer 768..788 /vntifkey="27" /label=oEMS2753 /note="note: there is a 1bp mismatch in this primer due to sequence differences based on expected vs. sequence verified regions" primer 54..73 /vntifkey="27" /label=oEMS2364 misc_feature 139..3658 /vntifkey="21" /label=LongProm\Region BASE COUNT 5866 a 5026 c 5075 g 6390 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccgg aacacctatt aatgcccaca tatctcaaac aaggaaatat 181 tgatcctgcc tatccctctg ctctctatta tatataaagc aaacaaagtc atttatccta 241 ggctgtctca taaaaacatt ttgtagcttc cacagtagca ttgcaaagcc tatattatga 301 ggaattttcc tctgaaatac attccaaatg gttcgtgttt aagttttatt atcccatcca 361 ttgttgtatc ttcaccagaa ataaaatgat ttttagttct gatgcaaaat taattgctac 421 atatttactg aatgcttatg atgtgcaaga aagaagtttt aagacattag ctttgttgga 481 atttccatat tttcctgccc ttattccttg atttttcttt gcctctagcc tgaataatgt 541 tgatttttca tgaggttgcc acaaggtaag gcaacaacac atggaagaaa atagggcaga 601 cacatgcatt cccctgaggt acttgtatag taaccaagaa atcagcagca aggtgttggg 661 gggacttaac taggtaggtt gcacatttga ggctatccac tcaactaaca gacagttcca 721 ggctttgctg agtcaacacc tttcacagaa gaaagaccat catatatttt atcccacttg 781 gcggcagctt acaataaaac acatgcagag aaaatgctta aatataaaag ttcaacagca 841 ctatataaaa ggaagggtta attatactag gaatcagata taaaataatt actgagcatg 901 ctcttagctc tgagcttcct ggaagccaag caaaaataga accatgatag ttcaaggctg 961 ctacatattg atgcatgtag cttcaattgt gaagatggta gcatccccct accccattta 1021 acctctcacc tttctctttt gttttatagt tcggcctgat ctaattagtt caatttggat 1081 gcttccttga gttttttttg taacatattt tatataaaga agtcagttag tgacaaataa 1141 gcagtttgag gagaaatctg ttaatattta ttttgtagcc atcagattta cttcacatag 1201 aaaggtcttt gggttgggtt tgaacttcca aactctcaaa ggtaaatgcc acattaacct 1261 ttcattaacc aaattcttac accaagctga tagatttggg atgtcctttt tacttctatc 1321 ttccataata ttctaaaatt attttccctt gttttgttcc tatcctactt cctcttagtc 1381 tactttgttg acttcattaa aaaacaaaaa accagttgtt ggatacttga gctaaactgc 1441 cttaaagaat ctgcagattt tattttattt tttttctctc aagagggtaa aaggaagaga 1501 gctacaattt ctaagaagcc tggcttggct gtctgagtct ggcccccagg cagattaggc 1561 caaggttttg gccaagtgaa attgccaatt ttctaaaaga aagggctagc acattgctca 1621 ttagagcatt ctgattttgt ctgcgcaatc tttctgctac cccgcaattt cctgttggtt 1681 ataaatgaaa cctttctagc tgttaatgca gcctgtgaat ttttttaaaa gcatgtaatt 1741 aatcatagga ggttgggggg attcactaag cctgagttac atgggagaag ctggacaagg 1801 cactaggacc tagaaggcat ctatccaccc tggcaggaat ttcttgcttg gagctcagac 1861 aacaaaggca tagagagatt ggttttcttt ctctcagcat ctccacccaa ccagcagaaa 1921 accggtgagt ggggctttta agtgattttc aagaagaatg taacagatgt caaacgggaa 1981 aagcacaagg caaagcctgc tctctctgtc tctctgtctc ctcttctcct tttttgcctt 2041 attctatccg attttttccc taagcttcta cctgggattt tcctttggaa aagtgagttt 2101 gatgttcctt tgttttcact gtgatgttaa tttagaataa tactacctct gatcctaaag 2161 caaagcaaag ccttactggc atgcctgggg aaatgtttgc tgcttgcctt gaggaggtgg 2221 ggtctcttac cactgcaggt tgtctgacag agacaatgct gagctcagca taggtcatgg 2281 tgacattgga aaaaaggcgg aattgagcct ggcagaccca ttaggcacca gtctttctta 2341 tctcctgtcc tcctggtccc ttgcaaatat attgatgtgg cagtgtgtag cagctgagcc 2401 ctgcttgctt tgtgagtcct tttatcccca tctgtgagat gcatgttaat agtttggctc 2461 gtaggatgtc actacatttg ctagcatttg tggcttcagt tgtattgggt ttcatgtttt 2521 gattgtttgg ggttcttggt gggggagggg gttcaacaga agggagaaaa gcaaagcctg 2581 acaaatgacc atcttttctc agctaatgca cctgggcaat atacaagttt ggggtgaatt 2641 gcctgctgtg agggtaaatg tcacttcaat taaggtagaa acccagaaca atgaaaggtg 2701 tgcttccttc taaaggtccc gtatgctgtt cggagagtca tttgtgaatc tttcaacaat 2761 taaattattc cattaagagg tgttgctgca tcagtgggga gggggtggag cacctggggg 2821 ggaaaaaaaa ggattttgtg aacaaatgga accgggggaa gacagagcta gtaacttgtt 2881 aaataactta tttttctaat cctttttccc cccagcttat ttcttatgaa tgtcggatag 2941 ctgcaccagc ttggtgggga aagggtttga tgaatagcac aaagacactg gctgttccct 3001 ggaggctgtc cctttaaagg agaatcttag tttattctgg ggggagggga tgcacacatt 3061 agagtaggaa agagggcttg gaataaaatg aaaacactcc cccttcatag tcattgtact 3121 gaaatgcaaa gactgcttcc taagctggag atgctaacct tgggtagctc cttctgttct 3181 cttcaagggg aattttgtca ggctatggat tcatttacaa ctgttagtca tgtgggcatg 3241 tgtgaggaaa cagatgccag ttttaatgta tttagcccga agttccaatt tgataggagc 3301 cactgtcagt aagtctcagg attttcagct atttcaaaat ctccccttct cctctgtctg 3361 gaacagtgcc aagagtgcct ccctctctat ctcttactcc caacccccac aaccaccagc 3421 acccccgccc agcccctcct tcttctctat taagatcaat attcctgcag gtcaggggca 3481 agcagcagat gggtcacagg cttttttcaa ccagttcttt tcacaagcag cagattgcag 3541 atctggatct ggctaatatt taaaatccct tcttttttcc ttctccttgt ccctttttgt 3601 ttttgcctct cttcaccccc atccctttct cccacgctca ggtctctgag gttccaccgg 3661 cgcgccgtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg 3721 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac 3781 tttgcctttc tctccacagg aagttcctat tccgaagttc ctattcttca aaaggtatag 3841 gaacttccgc caccatggcg gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg 3901 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 3961 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct 4021 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg 4081 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct 4141 acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga 4201 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc 4261 gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg 4321 gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg 4381 gagaaaaccg cctcgcggtg atggtgctgc gttggagtga cggcagttat ctggaagatc 4441 aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta 4501 cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac 4561 tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt 4621 tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg 4681 atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac 4741 tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg 4801 gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg 4861 gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc 4921 atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga 4981 agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca 5041 cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca 5101 tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg 5161 taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga 5221 atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc 5281 cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt 5341 gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca 5401 tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc 5461 acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc 5521 cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg 5581 aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc 5641 agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag 5701 caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca 5761 gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg 5821 gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga 5881 ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg 5941 tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt 6001 ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc 6061 tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc 6121 gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc 6181 tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc 6241 gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag 6301 cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc 6361 acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg 6421 gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg 6481 cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat 6541 tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc 6601 tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg 6661 ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca 6721 gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag 6781 aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga 6841 gcccgtcagt atcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct 6901 ggtgtcaaaa ataataaaag ttcctattcc gaagttccta ttctctagaa agtataggaa 6961 cttcagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt 7021 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 7081 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7141 ttcacaaata aagcattttt ttcactgcga attctcgtaa aactcttcat ggcagtagtt 7201 attattctct ctctctctct ttttcttttt tcttgagaca ggatatttct ctgttgccca 7261 ggctggagtg cagtggcaca gtcttggctc actgcagcct ggacctcctg ggctcaagcc 7321 atcctcccac ctcagcctcc caagtagctg gggctacagg cacatggcca ccaggcccag 7381 ataatttttc atttttgtag agactgagtc tcaccatgtt acccaggttt attattctca 7441 ttttttagat gaagagactg aggtccagag aagctcaatg acttgcctag ttttacaaat 7501 ctcctgccat cacatacccc tcagcgtcct taataagagg gaggccacca actatgtgct 7561 gggcactgtg gtggatgctg gagctatagg gttgagtata taagaaatgg tgttgctgga 7621 gcaactgttg cttgcttacc tgacctatct gagaattaat tagcagggga acatattttt 7681 gttttcagat tcaatataag aacttgtgtg ggcaaaaata aagatcagta gtaataacag 7741 tagttcccat ttgctgactg tactgtccta agtgcatata tatatacata cacacacgca 7801 tacctatact cctctaatac tcaaaatgat cctgtttatg tattgttaat atgctcattt 7861 tatttttaaa tttttattta tttttatttt tatttatttt tgagacggag tctcattctg 7921 tcgcggaggc tgaagtgcag tggtgcgatc tcagctcagt gcgacctccg cctcccgggt 7981 tcaagtgatt ctcctgcctc agcctccgga ctagctggga ttacaggcgc ccgcctccac 8041 gcccagctaa tttttgtatt tttagtagag atggggtttc gccatgttgg ccaggctggt 8101 ctcgtactcc tgaccttgag tgatccacct gcctcggcct cccaaagtgc tgggattaca 8161 ggcatgagcc accgcgccgg gctaatatgc tcattttagt gaggcaaaaa tagaggctca 8221 gagtctgatt tgtacaaaac tacagagcag ttaagtgtcc tctcagatgt gtaccctgat 8281 ctgggtgact ctaggactct aggtctcaac tgttacaacc agttaagggt ttggggaagc 8341 actgggccaa gagtcaggaa aatggaagcc acaggtagtg caaggtcttg ggaatgggac 8401 gtctggtcca aggattcacg cgatgactgg aacccgaaga gccggggccc ggtttacggc 8461 cgccatgaag caacgcgcgc cggtaggttt gggaatcagg gagccctctg aataggagac 8521 tgagttggga gggaaagggg cttcgctggg ggagcctcgg cttcttctgg gagaaaattc 8581 ccacggctac ctagtgagcc tgcaaactgg taggcgccgg cgtaggcgcg cgggcggggc 8641 cgggggcggg gcctgcgggg cgtggcgggg cgggcagagg gcggggcctg cttctcctca 8701 gcttcaggcg gctgcgacga gccctcaggc gaacctctcg gctttcccgc gcggcgccgc 8761 ctcttgctgc gcctccgcct cctcctctgc tccgccaccg gcttcctcct cctgagcagt 8821 cagcccgcgc gccggccggc tccgttatgg cgacccgcag ccctggcgtc gtggtgagca 8881 gctcggcctg ccggccctgg ccggttcagg cccacgcggc aggtggcggc cgggccctga 8941 ggcgcgggat cgatccatga cacccccccg ccccccatat ctcactataa ttcactgtca 9001 aatggctttt atgatttgga gtttggggat acttttaaag acagggtctt ggtagctcct 9061 agatggcttg ggattcactt agtaaccctt gctggcctaa aattcaggaa cttctacctc 9121 atcttcccaa gtactgggtt agtagggatg tccagcatat ttagcttgct ttaatgattt 9181 tgctgtactt ttttctgtgt gtacaggtgc atgtatattt ataaaactaa cataatatat 9241 tttaccccca aagtaccata accctaatta atgctattaa atgttttgta tgctgggctc 9301 tgggcatact aatataacca atattataca atgcaaaaca attttggaat atcttctgta 9361 aaattatctt attagtaagt tgtaaccatc tcagacttct aagtttgagt ttgtaaagga 9421 tcagtttttt ttggtgacct acacacacac acacacacac acacacacac acacacacag 9481 gggggtggat tctgagaatt tagagaattt agatggttaa taccagtttt tatttttatt 9541 ctgtgtgata tttatttatt aagatttatt tatttattac atgtaggtac actgtatctg 9601 tcttcagaca caccagaaga gggcatcaga tctcgttaca gatggttgtg agtcaccatg 9661 tggttgctgg gatttgaact caggacctct ggaagaacag tcagtgctct taaccgttga 9721 gccatctcac cagccccgtg atattttatt taacttacta gaatatactg tgttaaaaca 9781 tattttaggg gattggggag atggcccatt ctgtaaagtg tctgccatgt catcttcagg 9841 acctgcgttt gtaaccccag catggagaag acagagacag ggagacccct gggcttcctg 9901 gcaagctaga ctagcaaatt tggttaacta ggttcaatga gagactcagt ctcaaaacat 9961 aagaagtgaa ttttttagac ccaatttcaa tctctgaact ccatacgtat gcacatgtac 10021 ctgcatataa atatatatac acacccataa acacatgcac acacaaaaaa tggttcaaaa 10081 gttcttaaaa tgtataagag tagaaagtgt agagccggga gtggtggcgc acgcctttaa 10141 tcccagcact tgggagcaga ggcaggtgga tttctgagtt cgaggccagc ctggtctaca 10201 gagtgagttc caggacagcc agagctatac agagaaatcc tgcctcaaaa aaccaaaaat 10261 aaagtgtata tactaatctt tcatgcctaa aatatttatt tttgccacat ttttcatacc 10321 tctttaatat ttttcttttt ctctttgtgc ttcaaaggaa attttgtgtc atgttttact 10381 tttcattctt gactctattc agcagtaaga cgcagcatgg ctttcttaga gaagtataat 10441 ctattacaaa acggtaacat ttaactgctc tacataccct cttgtagcta aattcctctg 10501 ttaaactaag acttattatc taaacgtaat taaatgttga tatcttataa tgctccactt 10561 tgaaacagct gtctttcttt tgcagattag cgatgatgaa ccaggttatg acctagattt 10621 gttttgtata cctaatcatt atgccgagga tttggaaaaa gtgtttattc ctcatggact 10681 gattatggac aggttagtaa gacctcgatt gaagttattt ttagagggtg tgtgtgtggt 10741 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtaa gagagagagg agaggcatgt 10801 gtgatatata tgtgaagtgt attgcatgta catggaatcc agagaaggac attaggtgtc 10861 ctactctgtt gctctcttat tcccttgaat cagtgtctct tgctgaacct ggggcataca 10921 tactgatttg gctaggctgc ctggctagca agacccaggg atctgcttgt ctctgccccc 10981 cagtgctgac gttacaagca ggtgcaacca catctggttt ttatatgggt actgggggat 11041 ctgaacttag ggcctgatgc gtgcccaagt gctctcaccc gccaagccac ctctccagcc 11101 aaagggtttt ttttcttgta ttaattctaa cttagcaact gttgagtagt tgatgtttga 11161 atataagcta agccaactta ttagaataaa tgttttctat tttttacttg tatgtgtgag 11221 tgcctgcatg tatgtatgtg aagcatatgt attccaggat gtcagaattg tgatggattc 11281 cctggaactg gagttacaga ccattgtgaa ccactgtgta tgggtgctgg gaactgaact 11341 ttaggtcctc tgcaaaaaca gtaaggatct taactgataa actatctttc tagccctaag 11401 gctttctact tttaaagttc gtttctcttt ctctctctct ctctctctct ctctctctct 11461 ctccctctcc ctccctcctc ctcctcctcc tctttccccc ctctcgcctc ctctttctct 11521 ctcccctccc tctctctctc tcacacacac acacagacag agagaggaga gagaaaggaa 11581 gccttactaa ttaattacct agttttagac ataactagtg ggaatccata gtaagtaaat 11641 caacattctt gaagagaggt tagaagaatt ggctctcgtt gtccatcgag gctgtataag 11701 attattcttc tgttttctca gcttaccata catattttct acgtagacaa taccaattca 11761 aatacactgt acgtaaagta tatgcgcaca cacacacaca cacacacaca cacacacaca 11821 caagttacat attgtcatat agtactaaaa tttatggact ttttcatatt aatacatcta 11881 gagctatcat gttttttatc ttttaaaaat tactttacgg ggctacagag atgtctcagc 11941 agttaggagc actgactgct cttccagagg tcctgagttc aattcccagc aaccacatgg 12001 tggctcacaa ccatctgtaa tgggatctga tccactctta tggtgtgtct gaagacagtg 12061 acagtgtact catatacatg aaataaatct ttaaaaaatt actttaccat cagggtatat 12121 atatatatat atatgtgtgt gtgtgtgtgt gtgtgtatga agtatttgct atttgagagg 12181 gtctcactgt ttagctcagt ctggcttgga acgttctgta tagcccagac tggcctcaac 12241 tcagcagtcc ccctgaaagg ctctcatatg tggagattac aggtatgtgc tactgtgccc 12301 agccctacat gttactttgt ttttgaaata gggtatcaaa tagcttagac tggcttctaa 12361 gtcagtgtgt agctaaggat ggtctgagct tctgaacctg ctacttcaac tcctggtgtg 12421 ccatcatggc cagtttgcac agttgtgaga gtagaactca ggacctccta tgtgttacta 12481 gacatgttct ctaccagctg agctacatcc caattcgcct acatttactt ttatttttta 12541 aaagatttat ttatgtatac agcactctgc ctgcatgtac atctgcatgc cagaagaggg 12601 caccagatct catcatacat agttgtgagc caccatgtgg ttgctgggga ttgaactcag 12661 ggcctctgga agagcagtca gtgctcttaa cctctgagcc atctctgtag ccccctacat 12721 ttattttaaa gattataggc tattctattc atagatgtgc cataactgat ttaaccagat 12781 cttcattatt ttgtacttag tagagtatat tagagggaat gaatcttaaa tacacatttt 12841 gacacttgct cagctttaga caagtaaaaa tcactggtca aggaaatggt gcttagtgga 12901 tgcttctgaa ctgtcaaatt gtcctccaac aagttacatc attttatact tccaacgtta 12961 tgtgagagtt catttttctt taggctcttc tagtatttta tatttgagaa ataaaattta 13021 accaaaattt gccaatctag taagcttgtt tttatttgta tctttaaatt ttatgataga 13081 tgttatagtg tactctcctc tcccctcccc tcccctctcc tcctcagcag gatcttgtta 13141 tgtagcccta agggatctaa tacttactct gtagtccagg ctgtccttca gctcatgaga 13201 cacaccatca tgcttggcat gtttcttgtt ctgaaaggta tctggtttta attttggttg 13261 agctggtgtg ttggtgtata tctgtaatct cagcacaggc aagtagagac aaggattatg 13321 attacagtcc agcctgggct atttagaggg attctcttta atctttcctc atgccccaaa 13381 atcttacctt tggtatatga aaaatagtct ccacttctgc aaaatattgc tttatgaagt 13441 aagaattaat tcccttcata gagacaagga atgtgtcctg taaaagttta atgtgtaaga 13501 agtatttgtt ataaaagata aatattcaga atcttctttt taattcctga ttttatttct 13561 ataggactga aagacttgct cgagatgtca tgaaggagat gggaggccat cacattgtgg 13621 ccctctgtgt gctcaagggg ggctataagt tctttgctga cctgctggat tacattaaag 13681 cactgaatag aaatagtgat agatccattc ctatgactgt agattttatc agactgaaga 13741 gctactgtgt aagtataatt aacttataat taaaaaaata gggccattct agttttatct 13801 atattttttt taaacttgtg caaactatgc tacataatca acttacttga ttatgtaata 13861 tatatagtaa tagttggaat tactattatt acatatttat ttttctgtgc tggggtagaa 13921 cccagggaac taagtactgt aataaccgag cttcattcat cctagtcatt tttaattttt 13981 tttttttttt ttgttttttc gagacaaggt ttctctgtgt agccctggct gtcctggaac 14041 tcactctgta gaccaggctg gcctcgaact cagaaatctg cctgcctctg cctcccagtg 14101 ctgagattaa tgacgtgcac caccactgcc tggcattttt ttaaagtttt aatttgagac 14161 cctttctcac taacttgctc acactatcct cgaactcatt acgtgggctg gtctagaaat 14221 ttcagtcaac ttgcctcagc ttctcaagta gctgggatta caggcaggtg ctaccacttc 14281 tggccatgat cattgttgtt gttgctatta ttattattat tatttcactg atacttcata 14341 tcacaacaca gaatagtaac tgtagttcat gaccaacaaa aaagttgtac agtttctctg 14401 ttgtcttatt ttgatctgga ttttgatttg tttcatggca ctgggcaggc taatctcaaa 14461 ctgcatggct caaatagtcc ttctgtctta atctcccatg ttctggaagt ataggtatca 14521 ctacacctag ctgtattttc atattttaaa aaaaacgcct aagaattgct attgaatgag 14581 gcggagagat ggctcagcag tcacaagtac ttgtagagga ctggggtttg gttactagca 14641 cctcaaagat ggcttacaat tatctgtagc tccaattcca gaggatctgt gtggtaaagc 14701 tgcatgtata tgggcatgca ggtaaaactg ctaaacatat aaaaatagct cagtcttaag 14761 agaattggtc gactttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 14821 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 14881 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 14941 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg 15001 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 15061 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 15121 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 15181 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 15241 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 15301 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 15361 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 15421 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 15481 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 15541 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 15601 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 15661 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 15721 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 15781 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 15841 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 15901 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 15961 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 16021 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 16081 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 16141 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccaggtcgag 16201 tagggataac agggtaatgg atcctcgttg ggcacctgca ctcatgtacg caggcccaca 16261 tatagatgta cacacgcata tcattcaaaa tagaaattat ttatacaagt agtgcgttgg 16321 agagctggct caggagttgt taatgcttgc tacccttgca aaggacccaa gttcaatttc 16381 cagcacctgt gttgggcatc tcacaaccac atgtaaatct agtttcaggg gatttcaccc 16441 ctcttctgaa ctcttcaggc acttataccc acaaatggac atctccacat agagacacat 16501 acttttaaaa aagtaatcta gggtctagag aaatggttat agaccacgga ctgggatcag 16561 ttcccagaag ccacatcctg tggctcacaa ttgcctgtga atcaagttct agatctggtg 16621 gcctcgtcta gcctgcagtc atcaacaaac acatgcacac atccatacac acataattta 16681 aaagaaagcg aaagtaacct ataatgctaa acaaagcaca ttctcagtgt agactgcagt 16741 ggtgatgaac cttgaaaata tattaataga agccagacac gtaagtcaca aatggtataa 16801 ctcaatttat atgaaattcc cagagagtcc aaagttaaaa aaaacaaaaa ggcagattat 16861 tggttgtcag tagctacaga gatgggacat tgaagatatt aataggtata gggttgagga 16921 ttcatggcct ttccctcttc catgttagca tgtctattgt tgttaacctt attcaactca 16981 tgttagggca atcatattga tgagacttaa tgagtatagc ttgacattcc tagaagacag 17041 agtctcacat cagactctct gatcctctga ctcttagaat ctttcaacac cctcttctga 17101 aatgatctct gagcctttgg ttgtgactga attatgagaa agaattttat gttgaatgaa 17161 ttttacttca gcatttttac aaaactgaag ctggggatgg cgtttcatgc gtgcagtccc 17221 taccatttgc ctataagcct ggaggaggca gaagtagaat taggcttttc agggacatct 17281 tcagctgcat catgagcttg aggacaactt gagctacata agattcccat ccccaccccc 17341 acacacacac acacatctag tatgtaccta gtatgcagtc ttttcatatg tttaaaatgt 17401 agaaaggtat atgtgaagtc tcccttttcc ccacaactta gtccctccaa agtattgcat 17461 cagtttcctt gtacatgatt ttatgcatat ataagataca ctatttctct ctctctctct 17521 ctccttccct ccttccctcc aacctctggg tatgagttga gggttatgta tgtttcaagt 17581 tgtaataaga attgccactc aaacatttca tcccatttta agttaagttt gtctgatttt 17641 ccactttaga agggcagcca tgatggctca tgcctataat cccatcctag cacttgtaag 17701 cctaatacat aagactggag gattgtcatc agcttgaggc tagcctggtc tacctaatga 17761 gttccagcca gccaagagta gaatataaga ccctgtctcc aaaaaatgta ccaaaggggc 17821 tggaggggta actcagtggt taagaacagt agctgctctt ccagaggacc tgggttcaat 17881 tcccagcacc cacatggcag cttgcaacta tctgtaactg tccagctttg ggatatccga 17941 caccattata ccaattacat aaaatgaaaa aattgtaaaa tgtgctaaaa tgagtgggga 18001 gggggagcat ggtgtggttc tgtgggcttg ggattttgcc atgaatccat gactatatct 18061 cagcaatatg tggattattt ctggaaacct gtggggcatg ggtgtatggg aagaggggag 18121 agagagagag cccgcatcca gccagagttc ctgtgctctg ggcaggcgga cgtgggagga 18181 ctgctggacg atttccactt ggccctgggt gggcatctgg ctgtgtgaag ccactgaccc 18241 cactcagcgg ggggtagata aggggcattt cccataccag gttctcagac tccagcattc 18301 cacgctggat acagcagaag agctgagaac agaatagggg ccccagggag acactctcgt 18361 cccagagata aagaaggcag agggagagag attcctacgc aggcgagagg ccttagtctg 18421 ggccttgatg aacagagacg gtctatggtt ttagagcttt attgtagaaa ggcaggggga 18481 aagagagaag gtagaaagag agagaccagc catggccaag aggagagaag gggggaaagg 18541 agagagagaa gaaaggctag agagtaagaa aggttaaaga gacagaagta aaagcttaga 18601 gtaagagaga gagagagagc aagagggaga gtaagaaaag taaaagctta agagagcgag 18661 gaggggccag cccctctaat agtgggcttg ttatcttgtt gttgctaggt aactgtgaag 18721 cgtctagcct gaaggccaga agcttgggac attgtctatg tgactgaaag ccatgagcct 18781 ctactgtggg ggctgtggag gtggtaactt ggacaggagc caggggttcc aggagacatg 18841 agggaatgcc ttctgtccca tgtaggtagg aattaccacc accggctttc accgtagttc 18901 actggagact gtttgtacat agcccattgc cccacagaaa ccaggcactc actgtttcat 18961 ttgcataaga attagaaggc tttattttgg ctgggattgg tgacttgccc cagaaattcc 19021 agcttttggg aagccgaatc ctggagaacc aggagttcaa ggtcatcctc aggtacctca 19081 tttgttcaag accagcctgt gctaccatga gaccttgtcc ctactcccct caaccccaaa 19141 acacgctgtt ttcattttat aggttgtact aaccatggag gatggtcatt tgcctttctc 19201 ttgatttcag aagcgtagcg cgggctttgc accatgatct agtgtttcca gataggcttg 19261 cctctccaca gccaggctta tcctgcttca gtcccatgtt tggcaagggc aacatatgac 19321 tgaagaactt gcatagcctt atggtgtatc atgactctta gccccagttt ataactgtct 19381 aacagctggt ggcttttgtg aaaaaggaaa gcatcatttg aacttttaac ttttcagctg 19441 tttgttttga ctttgcataa atactgagac tagctaggaa atgagctttt gtttcatttg 19501 ctaaaaatga tttttttttc atgaggcaat ttcaagcaat ttgaatttag caatctctac 19561 ttgtctgaaa agaagttatt aattaaaaaa aaacaaacaa acaggaacct attatgctgg 19621 ctagtcacat gaaagaatcc aaatgaacac agtcaaggct gaatcaacac atgaaattat 19681 tttctaattc cccaaattac atttatgcta attagtatgt agtaaaacat tttaattgtt 19741 tccaatgtta aacattacag aaataatttt gatgttttcc taataaccca gcctttgatc 19801 atgtttgcat ttatttgatt gttgtcagtt tattaaataa ttaagtaaga gcaacaagat 19861 aaaatgactt catgagagtc cttttctgta acttagaacg tcagtagtca taggaactgc 19921 ggtcgttccc ttacctttca gaagtaggct aaagagttga acgcaaaggt ggtttttcga 19981 atcttcctga tccttaagcc cccactaggt ttatattcta tatttgatat cggattcgaa 20041 cccctcgagc ttaggatccg gaacccttaa tataactaag ttattaggtc cctcgaagag 20101 gttcactagt actggccatt gcggccgctt cgaggctgcc tcgcgcgttt cggtgatgac 20161 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 20221 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 20281 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag 20341 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 20401 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 20461 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 20521 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 20581 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 20641 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 20701 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 20761 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 20821 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 20881 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 20941 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 21001 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 21061 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 21121 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 21181 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 21241 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 21301 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 21361 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 21421 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 21481 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 21541 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 21601 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 21661 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 21721 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 21781 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 21841 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 21901 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 21961 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc 22021 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 22081 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 22141 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 22201 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 22261 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 22321 ttccgcgcac atttccccga aaagtgccac ctgacgg //