LOCUS Ple34\(pEMS1505) 22682 bp DNA circular 21-DEC-2009 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2364 - 5' - GCGTATCACGAGGCCCTTTC- 3' Tm=56.0 Deg C Antisense primer: oEMS3630 - 5' - GGACCCCTTTGACCCATGAATACA - 3 Tm=59.7 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 301 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|536514604| COMMENT VNTDBDATE|538223518| COMMENT LSOWNER| COMMENT VNTNAME|Ple34 (pEMS1505)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList) (CObjectList)) COMMENT #194=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -255 100) COMMENT (CObjectList) (CObjectList)) COMMENT #195=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -395 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) COMMENT (CObList #196=(AnlzFontColorItem 330 353 33554687) COMMENT #197=(AnlzFontColorItem 53 72 33554687)) 1 COMMENT (CObList #198=(AnlzFontBackColorItem 138 1161 50331392) COMMENT #199=(AnlzFontBackColorItem 1162 2312 33619967) COMMENT #200=(AnlzFontBackColorItem 2313 3982 33619712))) (CObList) 0 COMMENT (CStringList) 21972360 21902384 (CObList))) FEATURES Location/Qualifiers misc_recomb 16545..20356 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(21455..22676) /vntifkey="22" /label=AmpR misc_marker 15094..16519 /vntifkey="22" /label=AmpR misc_recomb 11453..15098 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 7499..11452 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 16526..16543 /vntifkey="21" /label=I-Sce\I misc_feature 4125..4172 /vntifkey="21" /label=FFRT\(F5\mutant) misc_feature 7243..7289 /vntifkey="21" /label=FFRT misc_feature 7443..7493 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 4173..4183 /vntifkey="21" /label=Kozak\consensus misc_feature 3992..4124 /vntifkey="21" /label=Intron misc_feature 4184..7242 /vntifkey="21" /label=lacZ misc_feature 139..3983 /vntifkey="21" /label=Ple34\(CLDN5-C) /note="1313" primer 331..354 /vntifkey="27" /label=oEMS3630 primer 54..73 /vntifkey="27" /label=oEMS2364 misc_feature 139..1162 /vntifkey="21" /label=Region\1 misc_feature 1163..2313 /vntifkey="21" /label=Region\2 misc_feature 2314..3983 /vntifkey="21" /label=Prom\Region BASE COUNT 5833 a 5262 c 5427 g 6160 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccac acagaagtat agagagaaga gtagaatgga tgcccatgtg 181 cccagcatcc agcctcagga actaccagcc tcctgctgtt ttattttctc tatccatcct 241 tcacatcttt tttttttctc gttttactca caaatactta agtatgtaag tagatacact 301 tcacccacat aatagagaat actctataca tgtattcatg ggtcaaaggg gtccctgaga 361 tacacacaat tcatactcat ccctctgaac actgcaagcg tcgtgtggag ccatttgctt 421 tcccacacac tcgctttctg ccgcagcaag gctggaccct gctggagtgg tgatgtgcag 481 gaactgcctc atagctcagg tggtaaagtt atttggctgg gaactgagtc tctccccaca 541 tgctgtcaca cccttcccgg ccctgacaaa cacaaagaga tgcatcccat tgagtcaccc 601 acttggctga accgtgtacc tggtaacctc cttgggaggt tctttggagg gtatttttct 661 cctctacaca aagcatgcct gcgaagtaag ctgagaatga caaaaacagg aaagcccaat 721 cttgcagaga cagaggggcc aaggcctgca tgataatgcg gcccaaattc atagctcaga 781 cccaaaagaa aacaaatgtt ccattctttc tgtgtccacc tctaaactaa gcaatacagt 841 gagggcagta aattgcaatt acaagtaaga actttagcag ttgctgatca taaaatgaaa 901 agggacaaat atgtttatcc ttaacagggt cacaaataat tccacagctg ttaattttgg 961 cagccacgac tctgcaggca gtatgggctc atggtatact atcttcattg tcaccttgga 1021 caaagatcct gttggccaaa ggcacacagg catcttcagg aaaactagat ttaaatggta 1081 agattaaaaa acacagtcat tatttaaaag cacaagaaag gtttcttttt taaagttcac 1141 taacaaatcc agagagtgat gaccggccta tgcttatgtt tttaagctcc aaagactact 1201 ggttctggga aggactgggt cactatgtga ccttgtgtag gtctcttttg gccctgagtc 1261 caggagggct ctgtgactcc aggttgtggc tgcgtctggc agctaacaat tgtttagttt 1321 acagtgtcct tcctcttcat cttgtttgca ttcagttgcc catgcctgcc tctgggggga 1381 ggtgggaagg aatgtggctc ctgggaggag cccagctcca tgtgccattc tgaggagcca 1441 ggctgcacgc ccctgggcca tagctgctgg tggatggatg agtacactgg cagaggcact 1501 gggaagggaa gacagaattg ttggccaata gtcccctttt actgattttc ttgtcatttt 1561 tgtgtttgtt ttattataaa gataacagct gtatttgaga aagaaagctc aaactataaa 1621 gaaaatttaa aattgccctg tcttactcca actccattcc ctgaaggtgg cctgtcttaa 1681 ctatttttgt ttctaattct tacagtggtt gccttcgtaa ttctaaagca tacaacccac 1741 atcttgattt ctcaactgtg tagtttcttt tggctctatt gtatgaaatc aatgtgaagt 1801 taacatattt agacaacctc tcctcctctc aacctctcaa tttgttcatt acttttagat 1861 cttgtaatcc agtgctttca aaatgcaggt tcccaggccg cagacaaact cattggaatt 1921 tgtggtggga cctaggaatg tgtatttata atagtctccc ctagttaatt gtgttcacta 1981 aagtttgaaa accatcatcc ttataactaa ataagtgtct taggagttgg ggcatttttg 2041 tctgccctat cagaaaccca gctaaggaag gacatcagtg aagatggagg atgaagaaat 2101 acaaatttca cccctccaca aaaaacaatg aaaaaaggga aaaaactcaa aaatcaactt 2161 tttaaaaata gctctggaca ttaaaaggct tgcagcaggc caggcatggt ggctcatgcc 2221 tgttatccca gcactttggg aggccgagtc gggtggatta cttgagatca ggagttcgag 2281 accagcctta tagtgaaatc cagtatctac taacttgcct tcagaacctc cccacactag 2341 gtgagccaga cgctggcctt atctcattta ccatctcaga gccatctgaa gggggagaag 2401 ggaaccgggc cccaggaggg agaaagtcat caaacctccc acatctgtga ccagcctcag 2461 tgccatactt tttctatgga gggccctgtc caatggaact gagcacagac cagataaaag 2521 aactgggcac ccagtggcct cagtccaggg cctggagttc aaactttact ggaaacaaag 2581 gggccgagag agactgggga aagaactact agaaaggggc tggtgccccc atggggctgt 2641 gggttttgga gccgcgtgcc cccacctgag cctcaggggg cccggagtgt ccacaccagt 2701 ggacctttcg agaaatggct gggccattgt gcagaagaat gcccggaaat cccgcgcctc 2761 cctcctccag caaggatggg ggctcttcct cctggccagg aaactccaag ttggcttccg 2821 gagggtggcc tgggggctgg ggtgccaggg acaccatcgc cactggtggg agggcagggc 2881 acagcccctc cgtgtccctt tgtctctcct gtctgaaggc cagagcaggc tgctaggcct 2941 ggggccacca ctgcccctgg gtgctacacc cagtgtgctg ggtcactggg aacttcctga 3001 agtggtgtca cctgaactgg acccccaagg atggggtgcg ggcagtaccg caggaagagg 3061 agcagcccct gtgaagattg agaggtctgg gaagcccctg cggcttggga gagtgggggt 3121 cgccaggcag ggggaaagcc cctgtgccac cgctttttgc cagagactca ggctccagag 3181 aggcagtgag tggcatgggg ggtgaggctg gggccctggg cctgacctcc acacgcctgc 3241 ctggcctctc tgtttgccat gggatgagag agacagtgct gggactcaga gcggggctgg 3301 agagtgagag tgcgagaaag ggcctgggtg gggcttggac cccggggcgg gctttctgga 3361 gagcccccct acgagggcct ctacggcggt gacggggtgg ggggcttctg caaaccttgg 3421 tcagggaagt ggagctggct cgagtggaag agaccacccg gctcagtcgg ggatgtggga 3481 gtggactggg tggtgcagac tgggggtcga gcgccttctg aagtgacggg gccgggacgc 3541 gcagggaggc ggcccaagaa gcgcgcccta ggccagccca gaatgcgctc ggccgcgact 3601 aggacaacgg cgggtggggc tgggggcggc tgccgggcgg ggagcggtcc cgcgccctca 3661 gctacccctc aagagccgtt gtttccctaa cttcagctgc cagaggctct gtgattggct 3721 gcggcacgat gacccgcgca cggattggct gcttcgggcc ggggggccgg gcccggggga 3781 cagaatccgc ccccgaacct tcaaagaggg taccccccgg caggagctgg cagacccagg 3841 aggtgcgaca gacccgcggg gcaaacggac tggggccaag agccgggagc gcgggcgcaa 3901 aggcaccagg gcccgcccag ggcgccgcgc agcacggcct tgggggttct gcgggccttc 3961 gggtgcgcgt ctcgcctcta gccggcgcgc cgtaagtatc aaggttacaa gacaggttta 4021 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc 4081 acctattggt cttactgaca tccactttgc ctttctctcc acaggaagtt cctattccga 4141 agttcctatt cttcaaaagg tataggaact tccgccacca tggcggatcc cgtcgtttta 4201 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc 4261 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 4321 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa 4381 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag 4441 atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg 4501 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa 4561 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat 4621 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt 4681 gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgttgg 4741 agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac 4801 gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt 4861 aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt 4921 gactacctac gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc 4981 gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta 5041 cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg 5101 gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt 5161 ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt 5221 cgaggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg 5281 atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat 5341 tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat 5401 gaagccaata ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc 5461 tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg 5521 agtgtgatca tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg 5581 tatcgctgga tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc 5641 gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc 5701 ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc 5761 ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa 5821 tactggcagg cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg 5881 gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat 5941 tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc 6001 acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta 6061 tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc 6121 ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat 6181 gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc 6241 gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc 6301 gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc 6361 gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg 6421 ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc 6481 gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac 6541 gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag 6601 gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct 6661 gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc 6721 agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa 6781 gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta 6841 gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact 6901 gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc 6961 ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg 7021 cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc 7081 catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg 7141 gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaattcca gctgagcgcc 7201 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aaaagttcct attccgaagt 7261 tcctattctc tagaaagtat aggaacttca gcggccgcga ctctagatca taatcagcca 7321 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 7381 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 7441 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcgaattct 7501 cgtaaaactc ttcatggcag tagttattat tctctctctc tctctttttc ttttttcttg 7561 agacaggata tttctctgtt gcccaggctg gagtgcagtg gcacagtctt ggctcactgc 7621 agcctggacc tcctgggctc aagccatcct cccacctcag cctcccaagt agctggggct 7681 acaggcacat ggccaccagg cccagataat ttttcatttt tgtagagact gagtctcacc 7741 atgttaccca ggtttattat tctcattttt tagatgaaga gactgaggtc cagagaagct 7801 caatgacttg cctagtttta caaatctcct gccatcacat acccctcagc gtccttaata 7861 agagggaggc caccaactat gtgctgggca ctgtggtgga tgctggagct atagggttga 7921 gtatataaga aatggtgttg ctggagcaac tgttgcttgc ttacctgacc tatctgagaa 7981 ttaattagca ggggaacata tttttgtttt cagattcaat ataagaactt gtgtgggcaa 8041 aaataaagat cagtagtaat aacagtagtt cccatttgct gactgtactg tcctaagtgc 8101 atatatatat acatacacac acgcatacct atactcctct aatactcaaa atgatcctgt 8161 ttatgtattg ttaatatgct cattttattt ttaaattttt atttattttt atttttattt 8221 atttttgaga cggagtctca ttctgtcgcg gaggctgaag tgcagtggtg cgatctcagc 8281 tcagtgcgac ctccgcctcc cgggttcaag tgattctcct gcctcagcct ccggactagc 8341 tgggattaca ggcgcccgcc tccacgccca gctaattttt gtatttttag tagagatggg 8401 gtttcgccat gttggccagg ctggtctcgt actcctgacc ttgagtgatc cacctgcctc 8461 ggcctcccaa agtgctggga ttacaggcat gagccaccgc gccgggctaa tatgctcatt 8521 ttagtgaggc aaaaatagag gctcagagtc tgatttgtac aaaactacag agcagttaag 8581 tgtcctctca gatgtgtacc ctgatctggg tgactctagg actctaggtc tcaactgtta 8641 caaccagtta agggtttggg gaagcactgg gccaagagtc aggaaaatgg aagccacagg 8701 tagtgcaagg tcttgggaat gggacgtctg gtccaaggat tcacgcgatg actggaaccc 8761 gaagagccgg ggcccggttt acggccgcca tgaagcaacg cgcgccggta ggtttgggaa 8821 tcagggagcc ctctgaatag gagactgagt tgggagggaa aggggcttcg ctgggggagc 8881 ctcggcttct tctgggagaa aattcccacg gctacctagt gagcctgcaa actggtaggc 8941 gccggcgtag gcgcgcgggc ggggccgggg gcggggcctg cggggcgtgg cggggcgggc 9001 agagggcggg gcctgcttct cctcagcttc aggcggctgc gacgagccct caggcgaacc 9061 tctcggcttt cccgcgcggc gccgcctctt gctgcgcctc cgcctcctcc tctgctccgc 9121 caccggcttc ctcctcctga gcagtcagcc cgcgcgccgg ccggctccgt tatggcgacc 9181 cgcagccctg gcgtcgtggt gagcagctcg gcctgccggc cctggccggt tcaggcccac 9241 gcggcaggtg gcggccgggc cctgaggcgc gggatcgatc catgacaccc ccccgccccc 9301 catatctcac tataattcac tgtcaaatgg cttttatgat ttggagtttg gggatacttt 9361 taaagacagg gtcttggtag ctcctagatg gcttgggatt cacttagtaa cccttgctgg 9421 cctaaaattc aggaacttct acctcatctt cccaagtact gggttagtag ggatgtccag 9481 catatttagc ttgctttaat gattttgctg tacttttttc tgtgtgtaca ggtgcatgta 9541 tatttataaa actaacataa tatattttac ccccaaagta ccataaccct aattaatgct 9601 attaaatgtt ttgtatgctg ggctctgggc atactaatat aaccaatatt atacaatgca 9661 aaacaatttt ggaatatctt ctgtaaaatt atcttattag taagttgtaa ccatctcaga 9721 cttctaagtt tgagtttgta aaggatcagt tttttttggt gacctacaca cacacacaca 9781 cacacacaca cacacacaca cacagggggg tggattctga gaatttagag aatttagatg 9841 gttaatacca gtttttattt ttattctgtg tgatatttat ttattaagat ttatttattt 9901 attacatgta ggtacactgt atctgtcttc agacacacca gaagagggca tcagatctcg 9961 ttacagatgg ttgtgagtca ccatgtggtt gctgggattt gaactcagga cctctggaag 10021 aacagtcagt gctcttaacc gttgagccat ctcaccagcc ccgtgatatt ttatttaact 10081 tactagaata tactgtgtta aaacatattt taggggattg gggagatggc ccattctgta 10141 aagtgtctgc catgtcatct tcaggacctg cgtttgtaac cccagcatgg agaagacaga 10201 gacagggaga cccctgggct tcctggcaag ctagactagc aaatttggtt aactaggttc 10261 aatgagagac tcagtctcaa aacataagaa gtgaattttt tagacccaat ttcaatctct 10321 gaactccata cgtatgcaca tgtacctgca tataaatata tatacacacc cataaacaca 10381 tgcacacaca aaaaatggtt caaaagttct taaaatgtat aagagtagaa agtgtagagc 10441 cgggagtggt ggcgcacgcc tttaatccca gcacttggga gcagaggcag gtggatttct 10501 gagttcgagg ccagcctggt ctacagagtg agttccagga cagccagagc tatacagaga 10561 aatcctgcct caaaaaacca aaaataaagt gtatatacta atctttcatg cctaaaatat 10621 ttatttttgc cacatttttc atacctcttt aatatttttc tttttctctt tgtgcttcaa 10681 aggaaatttt gtgtcatgtt ttacttttca ttcttgactc tattcagcag taagacgcag 10741 catggctttc ttagagaagt ataatctatt acaaaacggt aacatttaac tgctctacat 10801 accctcttgt agctaaattc ctctgttaaa ctaagactta ttatctaaac gtaattaaat 10861 gttgatatct tataatgctc cactttgaaa cagctgtctt tcttttgcag attagcgatg 10921 atgaaccagg ttatgaccta gatttgtttt gtatacctaa tcattatgcc gaggatttgg 10981 aaaaagtgtt tattcctcat ggactgatta tggacaggtt agtaagacct cgattgaagt 11041 tatttttaga gggtgtgtgt gtggtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 11101 tgtaagagag agaggagagg catgtgtgat atatatgtga agtgtattgc atgtacatgg 11161 aatccagaga aggacattag gtgtcctact ctgttgctct cttattccct tgaatcagtg 11221 tctcttgctg aacctggggc atacatactg atttggctag gctgcctggc tagcaagacc 11281 cagggatctg cttgtctctg ccccccagtg ctgacgttac aagcaggtgc aaccacatct 11341 ggtttttata tgggtactgg gggatctgaa cttagggcct gatgcgtgcc caagtgctct 11401 cacccgccaa gccacctctc cagccaaagg gttttttttc ttgtattaat tctaacttag 11461 caactgttga gtagttgatg tttgaatata agctaagcca acttattaga ataaatgttt 11521 tctatttttt acttgtatgt gtgagtgcct gcatgtatgt atgtgaagca tatgtattcc 11581 aggatgtcag aattgtgatg gattccctgg aactggagtt acagaccatt gtgaaccact 11641 gtgtatgggt gctgggaact gaactttagg tcctctgcaa aaacagtaag gatcttaact 11701 gataaactat ctttctagcc ctaaggcttt ctacttttaa agttcgtttc tctttctctc 11761 tctctctctc tctctctctc tctctctccc tctccctccc tcctcctcct cctcctcttt 11821 cccccctctc gcctcctctt tctctctccc ctccctctct ctctctcaca cacacacaca 11881 gacagagaga ggagagagaa aggaagcctt actaattaat tacctagttt tagacataac 11941 tagtgggaat ccatagtaag taaatcaaca ttcttgaaga gaggttagaa gaattggctc 12001 tcgttgtcca tcgaggctgt ataagattat tcttctgttt tctcagctta ccatacatat 12061 tttctacgta gacaatacca attcaaatac actgtacgta aagtatatgc gcacacacac 12121 acacacacac acacacacac acacacaagt tacatattgt catatagtac taaaatttat 12181 ggactttttc atattaatac atctagagct atcatgtttt ttatctttta aaaattactt 12241 tacggggcta cagagatgtc tcagcagtta ggagcactga ctgctcttcc agaggtcctg 12301 agttcaattc ccagcaacca catggtggct cacaaccatc tgtaatggga tctgatccac 12361 tcttatggtg tgtctgaaga cagtgacagt gtactcatat acatgaaata aatctttaaa 12421 aaattacttt accatcaggg tatatatata tatatatatg tgtgtgtgtg tgtgtgtgtg 12481 tatgaagtat ttgctatttg agagggtctc actgtttagc tcagtctggc ttggaacgtt 12541 ctgtatagcc cagactggcc tcaactcagc agtccccctg aaaggctctc atatgtggag 12601 attacaggta tgtgctactg tgcccagccc tacatgttac tttgtttttg aaatagggta 12661 tcaaatagct tagactggct tctaagtcag tgtgtagcta aggatggtct gagcttctga 12721 acctgctact tcaactcctg gtgtgccatc atggccagtt tgcacagttg tgagagtaga 12781 actcaggacc tcctatgtgt tactagacat gttctctacc agctgagcta catcccaatt 12841 cgcctacatt tacttttatt ttttaaaaga tttatttatg tatacagcac tctgcctgca 12901 tgtacatctg catgccagaa gagggcacca gatctcatca tacatagttg tgagccacca 12961 tgtggttgct ggggattgaa ctcagggcct ctggaagagc agtcagtgct cttaacctct 13021 gagccatctc tgtagccccc tacatttatt ttaaagatta taggctattc tattcataga 13081 tgtgccataa ctgatttaac cagatcttca ttattttgta cttagtagag tatattagag 13141 ggaatgaatc ttaaatacac attttgacac ttgctcagct ttagacaagt aaaaatcact 13201 ggtcaaggaa atggtgctta gtggatgctt ctgaactgtc aaattgtcct ccaacaagtt 13261 acatcatttt atacttccaa cgttatgtga gagttcattt ttctttaggc tcttctagta 13321 ttttatattt gagaaataaa atttaaccaa aatttgccaa tctagtaagc ttgtttttat 13381 ttgtatcttt aaattttatg atagatgtta tagtgtactc tcctctcccc tcccctcccc 13441 tctcctcctc agcaggatct tgttatgtag ccctaaggga tctaatactt actctgtagt 13501 ccaggctgtc cttcagctca tgagacacac catcatgctt ggcatgtttc ttgttctgaa 13561 aggtatctgg ttttaatttt ggttgagctg gtgtgttggt gtatatctgt aatctcagca 13621 caggcaagta gagacaagga ttatgattac agtccagcct gggctattta gagggattct 13681 ctttaatctt tcctcatgcc ccaaaatctt acctttggta tatgaaaaat agtctccact 13741 tctgcaaaat attgctttat gaagtaagaa ttaattccct tcatagagac aaggaatgtg 13801 tcctgtaaaa gtttaatgtg taagaagtat ttgttataaa agataaatat tcagaatctt 13861 ctttttaatt cctgatttta tttctatagg actgaaagac ttgctcgaga tgtcatgaag 13921 gagatgggag gccatcacat tgtggccctc tgtgtgctca aggggggcta taagttcttt 13981 gctgacctgc tggattacat taaagcactg aatagaaata gtgatagatc cattcctatg 14041 actgtagatt ttatcagact gaagagctac tgtgtaagta taattaactt ataattaaaa 14101 aaatagggcc attctagttt tatctatatt ttttttaaac ttgtgcaaac tatgctacat 14161 aatcaactta cttgattatg taatatatat agtaatagtt ggaattacta ttattacata 14221 tttatttttc tgtgctgggg tagaacccag ggaactaagt actgtaataa ccgagcttca 14281 ttcatcctag tcatttttaa tttttttttt tttttttgtt ttttcgagac aaggtttctc 14341 tgtgtagccc tggctgtcct ggaactcact ctgtagacca ggctggcctc gaactcagaa 14401 atctgcctgc ctctgcctcc cagtgctgag attaatgacg tgcaccacca ctgcctggca 14461 tttttttaaa gttttaattt gagacccttt ctcactaact tgctcacact atcctcgaac 14521 tcattacgtg ggctggtcta gaaatttcag tcaacttgcc tcagcttctc aagtagctgg 14581 gattacaggc aggtgctacc acttctggcc atgatcattg ttgttgttgc tattattatt 14641 attattattt cactgatact tcatatcaca acacagaata gtaactgtag ttcatgacca 14701 acaaaaaagt tgtacagttt ctctgttgtc ttattttgat ctggattttg atttgtttca 14761 tggcactggg caggctaatc tcaaactgca tggctcaaat agtccttctg tcttaatctc 14821 ccatgttctg gaagtatagg tatcactaca cctagctgta ttttcatatt ttaaaaaaaa 14881 cgcctaagaa ttgctattga atgaggcgga gagatggctc agcagtcaca agtacttgta 14941 gaggactggg gtttggttac tagcacctca aagatggctt acaattatct gtagctccaa 15001 ttccagagga tctgtgtggt aaagctgcat gtatatgggc atgcaggtaa aactgctaaa 15061 catataaaaa tagctcagtc ttaagagaat tggtcgactt tttcgccctt tgacgttgga 15121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 15181 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 15241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt 15301 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 15361 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 15421 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 15481 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 15541 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 15601 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 15661 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 15721 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 15781 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 15841 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 15901 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 15961 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 16021 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 16081 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 16141 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 16201 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 16261 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 16321 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 16381 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 16441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 16501 aaaaaaccac cgctaccagg tcgagtaggg ataacagggt aatggatcct cgttgggcac 16561 ctgcactcat gtacgcaggc ccacatatag atgtacacac gcatatcatt caaaatagaa 16621 attatttata caagtagtgc gttggagagc tggctcagga gttgttaatg cttgctaccc 16681 ttgcaaagga cccaagttca atttccagca cctgtgttgg gcatctcaca accacatgta 16741 aatctagttt caggggattt cacccctctt ctgaactctt caggcactta tacccacaaa 16801 tggacatctc cacatagaga cacatacttt taaaaaagta atctagggtc tagagaaatg 16861 gttatagacc acggactggg atcagttccc agaagccaca tcctgtggct cacaattgcc 16921 tgtgaatcaa gttctagatc tggtggcctc gtctagcctg cagtcatcaa caaacacatg 16981 cacacatcca tacacacata atttaaaaga aagcgaaagt aacctataat gctaaacaaa 17041 gcacattctc agtgtagact gcagtggtga tgaaccttga aaatatatta atagaagcca 17101 gacacgtaag tcacaaatgg tataactcaa tttatatgaa attcccagag agtccaaagt 17161 taaaaaaaac aaaaaggcag attattggtt gtcagtagct acagagatgg gacattgaag 17221 atattaatag gtatagggtt gaggattcat ggcctttccc tcttccatgt tagcatgtct 17281 attgttgtta accttattca actcatgtta gggcaatcat attgatgaga cttaatgagt 17341 atagcttgac attcctagaa gacagagtct cacatcagac tctctgatcc tctgactctt 17401 agaatctttc aacaccctct tctgaaatga tctctgagcc tttggttgtg actgaattat 17461 gagaaagaat tttatgttga atgaatttta cttcagcatt tttacaaaac tgaagctggg 17521 gatggcgttt catgcgtgca gtccctacca tttgcctata agcctggagg aggcagaagt 17581 agaattaggc ttttcaggga catcttcagc tgcatcatga gcttgaggac aacttgagct 17641 acataagatt cccatcccca cccccacaca cacacacaca tctagtatgt acctagtatg 17701 cagtcttttc atatgtttaa aatgtagaaa ggtatatgtg aagtctccct tttccccaca 17761 acttagtccc tccaaagtat tgcatcagtt tccttgtaca tgattttatg catatataag 17821 atacactatt tctctctctc tctctctcct tccctccttc cctccaacct ctgggtatga 17881 gttgagggtt atgtatgttt caagttgtaa taagaattgc cactcaaaca tttcatccca 17941 ttttaagtta agtttgtctg attttccact ttagaagggc agccatgatg gctcatgcct 18001 ataatcccat cctagcactt gtaagcctaa tacataagac tggaggattg tcatcagctt 18061 gaggctagcc tggtctacct aatgagttcc agccagccaa gagtagaata taagaccctg 18121 tctccaaaaa atgtaccaaa ggggctggag gggtaactca gtggttaaga acagtagctg 18181 ctcttccaga ggacctgggt tcaattccca gcacccacat ggcagcttgc aactatctgt 18241 aactgtccag ctttgggata tccgacacca ttataccaat tacataaaat gaaaaaattg 18301 taaaatgtgc taaaatgagt ggggaggggg agcatggtgt ggttctgtgg gcttgggatt 18361 ttgccatgaa tccatgacta tatctcagca atatgtggat tatttctgga aacctgtggg 18421 gcatgggtgt atgggaagag gggagagaga gagagcccgc atccagccag agttcctgtg 18481 ctctgggcag gcggacgtgg gaggactgct ggacgatttc cacttggccc tgggtgggca 18541 tctggctgtg tgaagccact gaccccactc agcggggggt agataagggg catttcccat 18601 accaggttct cagactccag cattccacgc tggatacagc agaagagctg agaacagaat 18661 aggggcccca gggagacact ctcgtcccag agataaagaa ggcagaggga gagagattcc 18721 tacgcaggcg agaggcctta gtctgggcct tgatgaacag agacggtcta tggttttaga 18781 gctttattgt agaaaggcag ggggaaagag agaaggtaga aagagagaga ccagccatgg 18841 ccaagaggag agaagggggg aaaggagaga gagaagaaag gctagagagt aagaaaggtt 18901 aaagagacag aagtaaaagc ttagagtaag agagagagag agagcaagag ggagagtaag 18961 aaaagtaaaa gcttaagaga gcgaggaggg gccagcccct ctaatagtgg gcttgttatc 19021 ttgttgttgc taggtaactg tgaagcgtct agcctgaagg ccagaagctt gggacattgt 19081 ctatgtgact gaaagccatg agcctctact gtgggggctg tggaggtggt aacttggaca 19141 ggagccaggg gttccaggag acatgaggga atgccttctg tcccatgtag gtaggaatta 19201 ccaccaccgg ctttcaccgt agttcactgg agactgtttg tacatagccc attgccccac 19261 agaaaccagg cactcactgt ttcatttgca taagaattag aaggctttat tttggctggg 19321 attggtgact tgccccagaa attccagctt ttgggaagcc gaatcctgga gaaccaggag 19381 ttcaaggtca tcctcaggta cctcatttgt tcaagaccag cctgtgctac catgagacct 19441 tgtccctact cccctcaacc ccaaaacacg ctgttttcat tttataggtt gtactaacca 19501 tggaggatgg tcatttgcct ttctcttgat ttcagaagcg tagcgcgggc tttgcaccat 19561 gatctagtgt ttccagatag gcttgcctct ccacagccag gcttatcctg cttcagtccc 19621 atgtttggca agggcaacat atgactgaag aacttgcata gccttatggt gtatcatgac 19681 tcttagcccc agtttataac tgtctaacag ctggtggctt ttgtgaaaaa ggaaagcatc 19741 atttgaactt ttaacttttc agctgtttgt tttgactttg cataaatact gagactagct 19801 aggaaatgag cttttgtttc atttgctaaa aatgattttt ttttcatgag gcaatttcaa 19861 gcaatttgaa tttagcaatc tctacttgtc tgaaaagaag ttattaatta aaaaaaaaca 19921 aacaaacagg aacctattat gctggctagt cacatgaaag aatccaaatg aacacagtca 19981 aggctgaatc aacacatgaa attattttct aattccccaa attacattta tgctaattag 20041 tatgtagtaa aacattttaa ttgtttccaa tgttaaacat tacagaaata attttgatgt 20101 tttcctaata acccagcctt tgatcatgtt tgcatttatt tgattgttgt cagtttatta 20161 aataattaag taagagcaac aagataaaat gacttcatga gagtcctttt ctgtaactta 20221 gaacgtcagt agtcatagga actgcggtcg ttcccttacc tttcagaagt aggctaaaga 20281 gttgaacgca aaggtggttt ttcgaatctt cctgatcctt aagcccccac taggtttata 20341 ttctatattt gatatcggat tcgaacccct cgagcttagg atccggaacc cttaatataa 20401 ctaagttatt aggtccctcg aagaggttca ctagtactgg ccattgcggc cgcttcgagg 20461 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 20521 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 20581 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 20641 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 20701 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 20761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 20821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 20881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 20941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 21001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 21061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 21121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 21181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 21241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 21301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 21361 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 21421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 21481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 21541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 21601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 21661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 21721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 21781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 21841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 21901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 21961 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca 22021 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 22081 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 22141 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 22201 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 22261 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 22321 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 22381 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 22441 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 22501 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 22561 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 22621 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 22681 gg //