LOCUS Ple12\(pEMS1241) 20112 bp DNA circular 3-FEB-2010 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2871 - 5' CTACTATTTCTTGGAAGGGGTGACG - 3' Tm=56.4 Deg C Antisense primer: oEMS2873 - 5' CATGCCAAGTAAGGTTCAAAGGC - 3 Tm=56.7 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 312 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|543241045| COMMENT VNTDBDATE|544963999| COMMENT LSOWNER| COMMENT VNTNAME|Ple12 (pEMS1241)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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2853 3773 41943168))) (CObList) 0 COMMENT (CStringList) 0 1684625219 (CObList))) FEATURES Location/Qualifiers misc_recomb 13975..17786 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(18885..20106) /vntifkey="22" /label=AmpR misc_marker 12524..13949 /vntifkey="22" /label=AmpR misc_recomb 8883..12528 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4929..8882 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 3831..3841 /vntifkey="21" /label=Kozak\consensus misc_feature 3838..4557 /vntifkey="21" /label=EGFP misc_feature 4558..4593 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4594..4666 /vntifkey="21" /label=NLS misc_feature 4873..4923 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 3783..3830 /vntifkey="21" /label=FFRT\(F5\mutant) misc_feature 4672..4719 /vntifkey="21" /label=FFRT misc_feature 13956..13973 /vntifkey="21" /label=I-Sce-I misc_feature 139..3774 /vntifkey="21" /label=Ple12\(AVP-C) /note="1307" primer 2644..2668 /vntifkey="27" /label=oEMS2871 /note="note there is a 1 bp descrepancy between primer and sequence that was detected after primer design" primer 2933..2955 /vntifkey="27" /label=oEMS2873 misc_feature 139..1042 /vntifkey="21" /label=Region\2 misc_feature 1043..2853 /vntifkey="21" /label=Region\3 misc_feature 2854..3774 /vntifkey="21" /label=Prom\Region BASE COUNT 5162 a 4810 c 4702 g 5438 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggcccg ggcagtgctg acaccctggg tcaggggcta ggaaagaggg 181 aagtcatggg tggtggtagc ctttagggga agttcggggg aggaagaggg aggcatggca 241 tggctgggca gaggagccaa tggggtgggc cagaggggac caggctttgg aggaggctgg 301 gagaggctga aggcgctcct ggtcactgtc gccatccaga cagggatgca ggaaaatgag 361 ggatgcttcc ccggtgactg ggcttggggc tggataggga gaacggggca tcatggcctc 421 ccctgtgccc atggcgttct tgcatctgga ctggctgggg cagcagaggc tccatcctac 481 ctagcattgg aggctttcct catccagccc cagcctccca gccacaggcg cccaggcccc 541 cacacagaag atggccactg gtctgagcgc gcttgagtgg ggcatcctgt ggggaagttc 601 tgctgggaac ctggcctaat tctatagtgc tggacgtttc ctccatttcc agcagagctg 661 aaggaaatcc aatcacgatg tgcatgcaat tctgtccagg ctcaatgatg agcccttgag 721 caaattagac cacaccaggc tcagctaaaa gtctaatgcg ctatccattg cgccagagaa 781 ccggctgttg agcagatgag agtggccgct cggcaacccc cgcagcctct cttcctcctg 841 ctaggctcct ttagggtcct gaggcacctg ggtgtccgtg ctcgcctcta ggtctcaggc 901 ccctgccacc cacctgatag gtcataggtg gctgagcagg ggtcagggct ccagctgagg 961 ccgacaagct tggcgggggc caggggcaag gcaagagagg agacaggaaa tgggaagggc 1021 cggggttctg gatgggtagg gcggacccgc agaggtgtcg ctagaccgag ggacagggcg 1081 aattgggagg caggggaggg ggagaccaga ggccgagagt ggccttggag ggggtgggtt 1141 gaggatcctc cggcagaggg aaagaagtaa aggcagagag ggaaaggcct ggaggacaag 1201 acagcaagag acccagagac agagacagca gaatgttgga gagagatgca gagacattga 1261 cagtgacgga gagaagagtg agagaggcag aggggacagg ccgaggggtg accattctgt 1321 ccccattttc atcccaggag actgaaacac aaaccgctca gggacccacc ctggtcccgg 1381 gattcccagt gcaatgtctg attcacccaa ggcagtgctc gtgtgtagtg gcggtgggga 1441 catcactgtc tgtccagtgc acccaccatt agggcccctc agccagagtg ggggacccct 1501 aacagataca gccccacaca accccaagca cgcttggaca gaaacagaca agaaaataac 1561 tgctaagaac gtgtccgcaa cccttgacca caaccacaga gaccctgagc cccacattca 1621 gagccacaga cacccagaga aacacagaca cagccgacac gaggatgggc aggagcatgt 1681 atgtacaggc tcacacaagc aggagtgcac tcacattagc tcccagacat gaagaccttc 1741 cgccctttga catgagcccc tttggcgaaa agacgtccag cagtgctcgg cctcttcctc 1801 tgcatccctt gggttctctc acccccttct tgaacctgag gcctttctgg tcccatctgc 1861 ccctttctct cctttgtctc tcactctgct ctcgcctccc tgttctgcca gctcctgggc 1921 cccctcttgc ctctctcctt ggcccctcca cccctcccct tctgtcccct caccccctct 1981 gccacctccc atccttctct ctcttggcct cctcttttct ctgcatcctc tccttgggct 2041 tcctgctcca ggtcttggag ccacaagagg ctgcatctcc ttcgggatcc tccgcaaact 2101 ctgaggtcag gaggtagtgc aggaggaggg aagtccacct gggccctgac cccaatgccc 2161 aggcttacgg ccaagcctgg tgactcaagc agcccagcct cagcagccct gggcagagtc 2221 caggcacatg tcaaggtcag gctctcttcc acactcccag ggctcccctt ggctctcacc 2281 cctgacctgg ttctgggctc ctctgccagg cagtgcccgg cacgactggg tctagccagt 2341 ggggacaggg accatgcagg cttgggtcac tgccatgacc tcctgctcct gtttcatgtt 2401 gtccactcag ggccatcccc catctggctg gggctagagc aggaccaaac ctaataggaa 2461 tttattaact cttccagact gggaaatagg aagaggcagg tggcttggct gcactgaatt 2521 caatccaaag tgttagtgtc tggacttggg gacagtttta gaatgaggtc caggaacttc 2581 ctcttcctaa atacagattc agagactcct ttccctccac ttgtctttgt caaaacaatc 2641 aggctactac ttcttggaag gggtgacgaa tgtctgccca cccccaattt gcccaaatgt 2701 ccacctccct gtacagtctt tcactctttc tccctctcct tgagtcccct ggcccagcta 2761 cagagccatt tagcaaggag aggtctgccc ttgtctgctc agtctctaca gggcaaatgg 2821 gctgcggcaa acccaccaca aggggcaaag cagccctggt tgccacatac taaggtcctg 2881 gtgggggtag gaattgggac aaactgttgt caggtttcct ggggctcccc ccgcctttga 2941 accttacttg gcatggagtt ctttctcccc atagtagctc tccacactca tgtttctacc 3001 tgggaggggg tgacgggtca tgtgccaatg tgtccccaag gcccccagtt tcagggcctg 3061 agtccccatg ccctgctcta tgggggtgcg ggggtgtcat gtcttgcact ttccccagca 3121 acctgccacc cccctaccct gtgagaacaa gcccctctca ttctgtgtcc cttgactcca 3181 cgacggcggc tgccttgtcc acgaggcagg gattctcatc tctggtgcag cccctgtcct 3241 caccactctg ggcttgtgct gttcttggtc ccttatcttg ttgtcacagg gctaaccctc 3301 cccacacacc tggctgtccc ctgccccaca gctcctaggc cagggcctgt ccctgcccct 3361 aacacgcagc ccctcggttc ctcttaccct cttcttggac cctacgccat ttctgggtaa 3421 tcagaaagct cagagatggt ctccaggtga ccccccagtc tgtccctctc cctgaatcca 3481 gagcgctgca gtcacagtag aggcaattgc tgtcattccg cggcccatga cagcctggcg 3541 gcccgtaccc ctcccccatg atcccctgca cagacaggcc cacgtgtgtc cccagatgcc 3601 tgaatcactg ctgacggctg gggacctggc ggccgtgggc tcctggggag ccactgggga 3661 gggggtggcg gccgcgtctc gcctccacgg gaacacctgc ggacataaat aggcagccag 3721 cagaggcagc agcacagagc caccaagcag tgctgcatac ggggtccacc tgtgggcgcg 3781 ccgaagttcc tattccgaag ttcctattct tcaaaaggta taggaacttc cgccaccatg 3841 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 3901 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 3961 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 4021 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 4081 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 4141 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 4201 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 4261 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 4321 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 4381 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 4441 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 4501 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagttattt 4561 ctgaactgct gcccgggctg ctgcatggaa ccggatccaa aaaagaagag aaaggtagat 4621 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtaatata agaagttcct 4681 attccgaagt tcctattctc tagaaagtat aggaacttca gcggccgcga ctctagatca 4741 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc 4801 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt 4861 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 4921 tgcgaattct cgtaaaactc ttcatggcag tagttattat tctctctctc tctctttttc 4981 ttttttcttg agacaggata tttctctgtt gcccaggctg gagtgcagtg gcacagtctt 5041 ggctcactgc agcctggacc tcctgggctc aagccatcct cccacctcag cctcccaagt 5101 agctggggct acaggcacat ggccaccagg cccagataat ttttcatttt tgtagagact 5161 gagtctcacc atgttaccca ggtttattat tctcattttt tagatgaaga gactgaggtc 5221 cagagaagct caatgacttg cctagtttta caaatctcct gccatcacat acccctcagc 5281 gtccttaata agagggaggc caccaactat gtgctgggca ctgtggtgga tgctggagct 5341 atagggttga gtatataaga aatggtgttg ctggagcaac tgttgcttgc ttacctgacc 5401 tatctgagaa ttaattagca ggggaacata tttttgtttt cagattcaat ataagaactt 5461 gtgtgggcaa aaataaagat cagtagtaat aacagtagtt cccatttgct gactgtactg 5521 tcctaagtgc atatatatat acatacacac acgcatacct atactcctct aatactcaaa 5581 atgatcctgt ttatgtattg ttaatatgct cattttattt ttaaattttt atttattttt 5641 atttttattt atttttgaga cggagtctca ttctgtcgcg gaggctgaag tgcagtggtg 5701 cgatctcagc tcagtgcgac ctccgcctcc cgggttcaag tgattctcct gcctcagcct 5761 ccggactagc tgggattaca ggcgcccgcc tccacgccca gctaattttt gtatttttag 5821 tagagatggg gtttcgccat gttggccagg ctggtctcgt actcctgacc ttgagtgatc 5881 cacctgcctc ggcctcccaa agtgctggga ttacaggcat gagccaccgc gccgggctaa 5941 tatgctcatt ttagtgaggc aaaaatagag gctcagagtc tgatttgtac aaaactacag 6001 agcagttaag tgtcctctca gatgtgtacc ctgatctggg tgactctagg actctaggtc 6061 tcaactgtta caaccagtta agggtttggg gaagcactgg gccaagagtc aggaaaatgg 6121 aagccacagg tagtgcaagg tcttgggaat gggacgtctg gtccaaggat tcacgcgatg 6181 actggaaccc gaagagccgg ggcccggttt acggccgcca tgaagcaacg cgcgccggta 6241 ggtttgggaa tcagggagcc ctctgaatag gagactgagt tgggagggaa aggggcttcg 6301 ctgggggagc ctcggcttct tctgggagaa aattcccacg gctacctagt gagcctgcaa 6361 actggtaggc gccggcgtag gcgcgcgggc ggggccgggg gcggggcctg cggggcgtgg 6421 cggggcgggc agagggcggg gcctgcttct cctcagcttc aggcggctgc gacgagccct 6481 caggcgaacc tctcggcttt cccgcgcggc gccgcctctt gctgcgcctc cgcctcctcc 6541 tctgctccgc caccggcttc ctcctcctga gcagtcagcc cgcgcgccgg ccggctccgt 6601 tatggcgacc cgcagccctg gcgtcgtggt gagcagctcg gcctgccggc cctggccggt 6661 tcaggcccac gcggcaggtg gcggccgggc cctgaggcgc gggatcgatc catgacaccc 6721 ccccgccccc catatctcac tataattcac tgtcaaatgg cttttatgat ttggagtttg 6781 gggatacttt taaagacagg gtcttggtag ctcctagatg gcttgggatt cacttagtaa 6841 cccttgctgg cctaaaattc aggaacttct acctcatctt cccaagtact gggttagtag 6901 ggatgtccag catatttagc ttgctttaat gattttgctg tacttttttc tgtgtgtaca 6961 ggtgcatgta tatttataaa actaacataa tatattttac ccccaaagta ccataaccct 7021 aattaatgct attaaatgtt ttgtatgctg ggctctgggc atactaatat aaccaatatt 7081 atacaatgca aaacaatttt ggaatatctt ctgtaaaatt atcttattag taagttgtaa 7141 ccatctcaga cttctaagtt tgagtttgta aaggatcagt tttttttggt gacctacaca 7201 cacacacaca cacacacaca cacacacaca cacagggggg tggattctga gaatttagag 7261 aatttagatg gttaatacca gtttttattt ttattctgtg tgatatttat ttattaagat 7321 ttatttattt attacatgta ggtacactgt atctgtcttc agacacacca gaagagggca 7381 tcagatctcg ttacagatgg ttgtgagtca ccatgtggtt gctgggattt gaactcagga 7441 cctctggaag aacagtcagt gctcttaacc gttgagccat ctcaccagcc ccgtgatatt 7501 ttatttaact tactagaata tactgtgtta aaacatattt taggggattg gggagatggc 7561 ccattctgta aagtgtctgc catgtcatct tcaggacctg cgtttgtaac cccagcatgg 7621 agaagacaga gacagggaga cccctgggct tcctggcaag ctagactagc aaatttggtt 7681 aactaggttc aatgagagac tcagtctcaa aacataagaa gtgaattttt tagacccaat 7741 ttcaatctct gaactccata cgtatgcaca tgtacctgca tataaatata tatacacacc 7801 cataaacaca tgcacacaca aaaaatggtt caaaagttct taaaatgtat aagagtagaa 7861 agtgtagagc cgggagtggt ggcgcacgcc tttaatccca gcacttggga gcagaggcag 7921 gtggatttct gagttcgagg ccagcctggt ctacagagtg agttccagga cagccagagc 7981 tatacagaga aatcctgcct caaaaaacca aaaataaagt gtatatacta atctttcatg 8041 cctaaaatat ttatttttgc cacatttttc atacctcttt aatatttttc tttttctctt 8101 tgtgcttcaa aggaaatttt gtgtcatgtt ttacttttca ttcttgactc tattcagcag 8161 taagacgcag catggctttc ttagagaagt ataatctatt acaaaacggt aacatttaac 8221 tgctctacat accctcttgt agctaaattc ctctgttaaa ctaagactta ttatctaaac 8281 gtaattaaat gttgatatct tataatgctc cactttgaaa cagctgtctt tcttttgcag 8341 attagcgatg atgaaccagg ttatgaccta gatttgtttt gtatacctaa tcattatgcc 8401 gaggatttgg aaaaagtgtt tattcctcat ggactgatta tggacaggtt agtaagacct 8461 cgattgaagt tatttttaga gggtgtgtgt gtggtgtgtg tgtgtgtgtg tgtgtgtgtg 8521 tgtgtgtgtg tgtaagagag agaggagagg catgtgtgat atatatgtga agtgtattgc 8581 atgtacatgg aatccagaga aggacattag gtgtcctact ctgttgctct cttattccct 8641 tgaatcagtg tctcttgctg aacctggggc atacatactg atttggctag gctgcctggc 8701 tagcaagacc cagggatctg cttgtctctg ccccccagtg ctgacgttac aagcaggtgc 8761 aaccacatct ggtttttata tgggtactgg gggatctgaa cttagggcct gatgcgtgcc 8821 caagtgctct cacccgccaa gccacctctc cagccaaagg gttttttttc ttgtattaat 8881 tctaacttag caactgttga gtagttgatg tttgaatata agctaagcca acttattaga 8941 ataaatgttt tctatttttt acttgtatgt gtgagtgcct gcatgtatgt atgtgaagca 9001 tatgtattcc aggatgtcag aattgtgatg gattccctgg aactggagtt acagaccatt 9061 gtgaaccact gtgtatgggt gctgggaact gaactttagg tcctctgcaa aaacagtaag 9121 gatcttaact gataaactat ctttctagcc ctaaggcttt ctacttttaa agttcgtttc 9181 tctttctctc tctctctctc tctctctctc tctctctccc tctccctccc tcctcctcct 9241 cctcctcttt cccccctctc gcctcctctt tctctctccc ctccctctct ctctctcaca 9301 cacacacaca gacagagaga ggagagagaa aggaagcctt actaattaat tacctagttt 9361 tagacataac tagtgggaat ccatagtaag taaatcaaca ttcttgaaga gaggttagaa 9421 gaattggctc tcgttgtcca tcgaggctgt ataagattat tcttctgttt tctcagctta 9481 ccatacatat tttctacgta gacaatacca attcaaatac actgtacgta aagtatatgc 9541 gcacacacac acacacacac acacacacac acacacaagt tacatattgt catatagtac 9601 taaaatttat ggactttttc atattaatac atctagagct atcatgtttt ttatctttta 9661 aaaattactt tacggggcta cagagatgtc tcagcagtta ggagcactga ctgctcttcc 9721 agaggtcctg agttcaattc ccagcaacca catggtggct cacaaccatc tgtaatggga 9781 tctgatccac tcttatggtg tgtctgaaga cagtgacagt gtactcatat acatgaaata 9841 aatctttaaa aaattacttt accatcaggg tatatatata tatatatatg tgtgtgtgtg 9901 tgtgtgtgtg tatgaagtat ttgctatttg agagggtctc actgtttagc tcagtctggc 9961 ttggaacgtt ctgtatagcc cagactggcc tcaactcagc agtccccctg aaaggctctc 10021 atatgtggag attacaggta tgtgctactg tgcccagccc tacatgttac tttgtttttg 10081 aaatagggta tcaaatagct tagactggct tctaagtcag tgtgtagcta aggatggtct 10141 gagcttctga acctgctact tcaactcctg gtgtgccatc atggccagtt tgcacagttg 10201 tgagagtaga actcaggacc tcctatgtgt tactagacat gttctctacc agctgagcta 10261 catcccaatt cgcctacatt tacttttatt ttttaaaaga tttatttatg tatacagcac 10321 tctgcctgca tgtacatctg catgccagaa gagggcacca gatctcatca tacatagttg 10381 tgagccacca tgtggttgct ggggattgaa ctcagggcct ctggaagagc agtcagtgct 10441 cttaacctct gagccatctc tgtagccccc tacatttatt ttaaagatta taggctattc 10501 tattcataga tgtgccataa ctgatttaac cagatcttca ttattttgta cttagtagag 10561 tatattagag ggaatgaatc ttaaatacac attttgacac ttgctcagct ttagacaagt 10621 aaaaatcact ggtcaaggaa atggtgctta gtggatgctt ctgaactgtc aaattgtcct 10681 ccaacaagtt acatcatttt atacttccaa cgttatgtga gagttcattt ttctttaggc 10741 tcttctagta ttttatattt gagaaataaa atttaaccaa aatttgccaa tctagtaagc 10801 ttgtttttat ttgtatcttt aaattttatg atagatgtta tagtgtactc tcctctcccc 10861 tcccctcccc tctcctcctc agcaggatct tgttatgtag ccctaaggga tctaatactt 10921 actctgtagt ccaggctgtc cttcagctca tgagacacac catcatgctt ggcatgtttc 10981 ttgttctgaa aggtatctgg ttttaatttt ggttgagctg gtgtgttggt gtatatctgt 11041 aatctcagca caggcaagta gagacaagga ttatgattac agtccagcct gggctattta 11101 gagggattct ctttaatctt tcctcatgcc ccaaaatctt acctttggta tatgaaaaat 11161 agtctccact tctgcaaaat attgctttat gaagtaagaa ttaattccct tcatagagac 11221 aaggaatgtg tcctgtaaaa gtttaatgtg taagaagtat ttgttataaa agataaatat 11281 tcagaatctt ctttttaatt cctgatttta tttctatagg actgaaagac ttgctcgaga 11341 tgtcatgaag gagatgggag gccatcacat tgtggccctc tgtgtgctca aggggggcta 11401 taagttcttt gctgacctgc tggattacat taaagcactg aatagaaata gtgatagatc 11461 cattcctatg actgtagatt ttatcagact gaagagctac tgtgtaagta taattaactt 11521 ataattaaaa aaatagggcc attctagttt tatctatatt ttttttaaac ttgtgcaaac 11581 tatgctacat aatcaactta cttgattatg taatatatat agtaatagtt ggaattacta 11641 ttattacata tttatttttc tgtgctgggg tagaacccag ggaactaagt actgtaataa 11701 ccgagcttca ttcatcctag tcatttttaa tttttttttt tttttttgtt ttttcgagac 11761 aaggtttctc tgtgtagccc tggctgtcct ggaactcact ctgtagacca ggctggcctc 11821 gaactcagaa atctgcctgc ctctgcctcc cagtgctgag attaatgacg tgcaccacca 11881 ctgcctggca tttttttaaa gttttaattt gagacccttt ctcactaact tgctcacact 11941 atcctcgaac tcattacgtg ggctggtcta gaaatttcag tcaacttgcc tcagcttctc 12001 aagtagctgg gattacaggc aggtgctacc acttctggcc atgatcattg ttgttgttgc 12061 tattattatt attattattt cactgatact tcatatcaca acacagaata gtaactgtag 12121 ttcatgacca acaaaaaagt tgtacagttt ctctgttgtc ttattttgat ctggattttg 12181 atttgtttca tggcactggg caggctaatc tcaaactgca tggctcaaat agtccttctg 12241 tcttaatctc ccatgttctg gaagtatagg tatcactaca cctagctgta ttttcatatt 12301 ttaaaaaaaa cgcctaagaa ttgctattga atgaggcgga gagatggctc agcagtcaca 12361 agtacttgta gaggactggg gtttggttac tagcacctca aagatggctt acaattatct 12421 gtagctccaa ttccagagga tctgtgtggt aaagctgcat gtatatgggc atgcaggtaa 12481 aactgctaaa catataaaaa tagctcagtc ttaagagaat tggtcgactt tttcgccctt 12541 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 12601 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 12661 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 12721 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 12781 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 12841 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 12901 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 12961 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 13021 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 13081 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 13141 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 13201 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 13261 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 13321 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 13381 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 13441 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 13501 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 13561 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 13621 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 13681 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 13741 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 13801 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 13861 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 13921 cttgcaaaca aaaaaaccac cgctaccagg tcgagtaggg ataacagggt aatggatcct 13981 cgttgggcac ctgcactcat gtacgcaggc ccacatatag atgtacacac gcatatcatt 14041 caaaatagaa attatttata caagtagtgc gttggagagc tggctcagga gttgttaatg 14101 cttgctaccc ttgcaaagga cccaagttca atttccagca cctgtgttgg gcatctcaca 14161 accacatgta aatctagttt caggggattt cacccctctt ctgaactctt caggcactta 14221 tacccacaaa tggacatctc cacatagaga cacatacttt taaaaaagta atctagggtc 14281 tagagaaatg gttatagacc acggactggg atcagttccc agaagccaca tcctgtggct 14341 cacaattgcc tgtgaatcaa gttctagatc tggtggcctc gtctagcctg cagtcatcaa 14401 caaacacatg cacacatcca tacacacata atttaaaaga aagcgaaagt aacctataat 14461 gctaaacaaa gcacattctc agtgtagact gcagtggtga tgaaccttga aaatatatta 14521 atagaagcca gacacgtaag tcacaaatgg tataactcaa tttatatgaa attcccagag 14581 agtccaaagt taaaaaaaac aaaaaggcag attattggtt gtcagtagct acagagatgg 14641 gacattgaag atattaatag gtatagggtt gaggattcat ggcctttccc tcttccatgt 14701 tagcatgtct attgttgtta accttattca actcatgtta gggcaatcat attgatgaga 14761 cttaatgagt atagcttgac attcctagaa gacagagtct cacatcagac tctctgatcc 14821 tctgactctt agaatctttc aacaccctct tctgaaatga tctctgagcc tttggttgtg 14881 actgaattat gagaaagaat tttatgttga atgaatttta cttcagcatt tttacaaaac 14941 tgaagctggg gatggcgttt catgcgtgca gtccctacca tttgcctata agcctggagg 15001 aggcagaagt agaattaggc ttttcaggga catcttcagc tgcatcatga gcttgaggac 15061 aacttgagct acataagatt cccatcccca cccccacaca cacacacaca tctagtatgt 15121 acctagtatg cagtcttttc atatgtttaa aatgtagaaa ggtatatgtg aagtctccct 15181 tttccccaca acttagtccc tccaaagtat tgcatcagtt tccttgtaca tgattttatg 15241 catatataag atacactatt tctctctctc tctctctcct tccctccttc cctccaacct 15301 ctgggtatga gttgagggtt atgtatgttt caagttgtaa taagaattgc cactcaaaca 15361 tttcatccca ttttaagtta agtttgtctg attttccact ttagaagggc agccatgatg 15421 gctcatgcct ataatcccat cctagcactt gtaagcctaa tacataagac tggaggattg 15481 tcatcagctt gaggctagcc tggtctacct aatgagttcc agccagccaa gagtagaata 15541 taagaccctg tctccaaaaa atgtaccaaa ggggctggag gggtaactca gtggttaaga 15601 acagtagctg ctcttccaga ggacctgggt tcaattccca gcacccacat ggcagcttgc 15661 aactatctgt aactgtccag ctttgggata tccgacacca ttataccaat tacataaaat 15721 gaaaaaattg taaaatgtgc taaaatgagt ggggaggggg agcatggtgt ggttctgtgg 15781 gcttgggatt ttgccatgaa tccatgacta tatctcagca atatgtggat tatttctgga 15841 aacctgtggg gcatgggtgt atgggaagag gggagagaga gagagcccgc atccagccag 15901 agttcctgtg ctctgggcag gcggacgtgg gaggactgct ggacgatttc cacttggccc 15961 tgggtgggca tctggctgtg tgaagccact gaccccactc agcggggggt agataagggg 16021 catttcccat accaggttct cagactccag cattccacgc tggatacagc agaagagctg 16081 agaacagaat aggggcccca gggagacact ctcgtcccag agataaagaa ggcagaggga 16141 gagagattcc tacgcaggcg agaggcctta gtctgggcct tgatgaacag agacggtcta 16201 tggttttaga gctttattgt agaaaggcag ggggaaagag agaaggtaga aagagagaga 16261 ccagccatgg ccaagaggag agaagggggg aaaggagaga gagaagaaag gctagagagt 16321 aagaaaggtt aaagagacag aagtaaaagc ttagagtaag agagagagag agagcaagag 16381 ggagagtaag aaaagtaaaa gcttaagaga gcgaggaggg gccagcccct ctaatagtgg 16441 gcttgttatc ttgttgttgc taggtaactg tgaagcgtct agcctgaagg ccagaagctt 16501 gggacattgt ctatgtgact gaaagccatg agcctctact gtgggggctg tggaggtggt 16561 aacttggaca ggagccaggg gttccaggag acatgaggga atgccttctg tcccatgtag 16621 gtaggaatta ccaccaccgg ctttcaccgt agttcactgg agactgtttg tacatagccc 16681 attgccccac agaaaccagg cactcactgt ttcatttgca taagaattag aaggctttat 16741 tttggctggg attggtgact tgccccagaa attccagctt ttgggaagcc gaatcctgga 16801 gaaccaggag ttcaaggtca tcctcaggta cctcatttgt tcaagaccag cctgtgctac 16861 catgagacct tgtccctact cccctcaacc ccaaaacacg ctgttttcat tttataggtt 16921 gtactaacca tggaggatgg tcatttgcct ttctcttgat ttcagaagcg tagcgcgggc 16981 tttgcaccat gatctagtgt ttccagatag gcttgcctct ccacagccag gcttatcctg 17041 cttcagtccc atgtttggca agggcaacat atgactgaag aacttgcata gccttatggt 17101 gtatcatgac tcttagcccc agtttataac tgtctaacag ctggtggctt ttgtgaaaaa 17161 ggaaagcatc atttgaactt ttaacttttc agctgtttgt tttgactttg cataaatact 17221 gagactagct aggaaatgag cttttgtttc atttgctaaa aatgattttt ttttcatgag 17281 gcaatttcaa gcaatttgaa tttagcaatc tctacttgtc tgaaaagaag ttattaatta 17341 aaaaaaaaca aacaaacagg aacctattat gctggctagt cacatgaaag aatccaaatg 17401 aacacagtca aggctgaatc aacacatgaa attattttct aattccccaa attacattta 17461 tgctaattag tatgtagtaa aacattttaa ttgtttccaa tgttaaacat tacagaaata 17521 attttgatgt tttcctaata acccagcctt tgatcatgtt tgcatttatt tgattgttgt 17581 cagtttatta aataattaag taagagcaac aagataaaat gacttcatga gagtcctttt 17641 ctgtaactta gaacgtcagt agtcatagga actgcggtcg ttcccttacc tttcagaagt 17701 aggctaaaga gttgaacgca aaggtggttt ttcgaatctt cctgatcctt aagcccccac 17761 taggtttata ttctatattt gatatcggat tcgaacccct cgagcttagg atccggaacc 17821 cttaatataa ctaagttatt aggtccctcg aagaggttca ctagtactgg ccattgcggc 17881 cgcttcgagg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 17941 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 18001 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata 18061 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca 18121 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc 18181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 18241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 18301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 18361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 18421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 18481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 18541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 18601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 18661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 18721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 18781 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 18841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 18901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 18961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 19021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 19081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 19141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 19201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 19261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 19321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 19381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat 19441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 19501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 19561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 19621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 19681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga 19741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 19801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 19861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 19921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 19981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 20041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 20101 gccacctgac gg //