LOCUS Ple3\(pEMS1143) 18291 bp DNA circular 3-FEB-2010 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2364 - 5' - GCGTATCACGAGGCCCTTTC- 3' Tm=56.0 Deg C Antisense primer: oEMS2860 - 5' TGACACCCCTCAGAAGCAGAAGT- 3 Tm=56.7 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 386 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|543240837| COMMENT VNTDBDATE|544963224| COMMENT LSOWNER| COMMENT VNTNAME|Ple3 (pEMS1143)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#201=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #115# 47159068 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "HPRT complementary sequence" "@N" 1 18.6284 COMMENT -6.96932 0 -10 14.9609 1.17389 #200#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #202=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -396 100) COMMENT (CObjectList) (CObjectList)) COMMENT #203=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -256 100) COMMENT (CObjectList) (CObjectList)) COMMENT #204=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 958 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) COMMENT (CObList #205=(AnlzFontColorItem 395 417 33587200) COMMENT #206=(AnlzFontColorItem 53 72 33587200)) 1 COMMENT (CObList #207=(AnlzFontBackColorItem 152 918 33554687) COMMENT #208=(AnlzFontBackColorItem 919 1952 33554560))) (CObList) 0 COMMENT (CStringList) 0 1684625219 (CObList))) FEATURES Location/Qualifiers misc_recomb 12154..15965 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(17064..18285) /vntifkey="22" /label=AmpR misc_marker 10703..12128 /vntifkey="22" /label=AmpR misc_recomb 7062..10707 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 3108..7061 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 122..152 /vntifkey="21" /label=MCS misc_feature 2010..2020 /vntifkey="21" /label=Kozak\consensus misc_feature 2017..2736 /vntifkey="21" /label=EGFP misc_feature 2737..2772 /vntifkey="21" /label=Tetracysteine\residue misc_feature 2773..2845 /vntifkey="21" /label=NLS misc_feature 3052..3102 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 1962..2009 /vntifkey="21" /label=FFRT\(F5\mutant) misc_feature 2851..2898 /vntifkey="21" /label=FFRT misc_feature 12135..12152 /vntifkey="21" /label=I-Sce-I misc_feature 153..1953 /vntifkey="21" /label=Ple3\(ADORA2A-C) /note="1307" misc_feature 153..919 /vntifkey="21" /label=Prom\Region misc_feature 920..1953 /vntifkey="21" /label=5'\UTR\Region primer 396..418 /vntifkey="27" /label=oEMS2860 primer 54..73 /vntifkey="27" /label=oEMS2364 BASE COUNT 4769 a 4156 c 4314 g 5052 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccac gcgtggcgcg ccggctgccc gagtccacgt gaaaggcagc 181 ctctgacctc ttgtccccag gcaggtggtg gcggctggca acacactcat agggccccat 241 gagggttcag gattggaggg ggtagatttg ggggcctctt agctctcaaa gaggggcaat 301 ttaacgggca gaggtccatt tggatccaga ccattcacag gctcgaaggg gacactggga 361 gttggggctg ggctcaggcc tgctggggaa ccaaaacttc tgcttctgag gggtgtcagt 421 aattccattt tgactctggg ggagccattt taaatctgtt tatttccttc tcatagaatc 481 atgggtgaga gctggcatgg cccctagagg tcatttgggg tccagctgcc tcaccgtatc 541 aatgaggaaa ctgaggccca gaaaagaaaa gcatttttgc ccagagtccc tcagtgagtc 601 ctggttccgt acctgctttc tgccagggac caaactcccg ttagtcattc ctggtgtcag 661 ccaggcagag gagcaggtgg gggagagggc gtgggaggag ggaggtgagg cagctccctc 721 cgagtggaag ctcacagtca gctgcagcgc cgcccctgcc ctgactgcag ctgactggca 781 ggggcacctg gaggcagcgg gttcgggcgc agtatgagag ggcttaccct ctgcggcagt 841 gggagacagg accaggcagc tccccacatc cttccacatc cgagctccag gggtctctgg 901 cttgtccttt cacagggcgg ctggtgtccc ttaggtgggt gctggtagtt cctgggagag 961 ttgacgggat ggagcaggcc aggggggctg agaagttggc ctggcgagag aaggtgggtg 1021 ggactgggag agcctgggca gggaggggcc tttctgggag ggagtgtcat ggggctggcc 1081 gagatccagg acccaggaga ctggccaagc tgccacagga ggcctgagag gctgagcatg 1141 ggggggccaa cactgacaga ctttaacaag tttctggctc ccccaggaaa aaaacagacc 1201 acatctgatc cttggccctg agtccagagt gggaggcacc gtgacaacaa tgcgcagagc 1261 agggaatgca gggagccatg gatagtgctg gggtgagctg gagtcctggc aggggcccag 1321 ctagggcagt actcattctg cagagcctgg gagaaggggc tgcctccttg ggaggggttt 1381 gtgtgggagt ctcagcggga attctattgg accaggggaa ccagccttgc tcagtgactt 1441 ctctgggtgt cctcctctaa agtgagggtc aaattcccac ctctctcgcc tcctggctat 1501 gaccacagca gaccaggcag ggcatggggc taggcggggt caggggccat cctcaggctg 1561 acacctggcc tgtgggtagg gtgggatctg aaactatagg gtcttcaggg ggccaagtgt 1621 ggggtaaggg gtggcacttt ccgcagggct cgccctctgc agatggttca gcttctcatc 1681 ggctgtccct ttgcaggtgc ctcaggaacc ctgaagctgg gctgagccat gatgctgctg 1741 ccagaacccc tgcagagggc ctggtttcag gagactcaga gtcctctgtg aaaaagccct 1801 tggagagcgc cccagcaggg ctgcacttgg ctcctgtgag gaaggggctc aggggtctgg 1861 gcccctccgc ctgggccggg ctgggagcca ggcgggcggc tgggctgcag caatggaccg 1921 tgagctggcc cagcccgcgt ccgtgctgag cctggcgcgc cgaagttcct attccgaagt 1981 tcctattctt caaaaggtat aggaacttcc gccaccatgg tgagcaaggg cgaggagctg 2041 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 2101 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 2161 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 2221 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 2281 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 2341 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 2401 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 2461 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 2521 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 2581 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 2641 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 2701 gggatcactc tcggcatgga cgagctgtac aagttatttc tgaactgctg cccgggctgc 2761 tgcatggaac cggatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta 2821 gatccaaaaa agaagagaaa ggtaatataa gaagttccta ttccgaagtt cctattctct 2881 agaaagtata ggaacttcag cggccgcgac tctagatcat aatcagccat accacatttg 2941 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 3001 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 3061 atagcatcac aaatttcaca aataaagcat ttttttcact gcgaattctc gtaaaactct 3121 tcatggcagt agttattatt ctctctctct ctctttttct tttttcttga gacaggatat 3181 ttctctgttg cccaggctgg agtgcagtgg cacagtcttg gctcactgca gcctggacct 3241 cctgggctca agccatcctc ccacctcagc ctcccaagta gctggggcta caggcacatg 3301 gccaccaggc ccagataatt tttcattttt gtagagactg agtctcacca tgttacccag 3361 gtttattatt ctcatttttt agatgaagag actgaggtcc agagaagctc aatgacttgc 3421 ctagttttac aaatctcctg ccatcacata cccctcagcg tccttaataa gagggaggcc 3481 accaactatg tgctgggcac tgtggtggat gctggagcta tagggttgag tatataagaa 3541 atggtgttgc tggagcaact gttgcttgct tacctgacct atctgagaat taattagcag 3601 gggaacatat ttttgttttc agattcaata taagaacttg tgtgggcaaa aataaagatc 3661 agtagtaata acagtagttc ccatttgctg actgtactgt cctaagtgca tatatatata 3721 catacacaca cgcataccta tactcctcta atactcaaaa tgatcctgtt tatgtattgt 3781 taatatgctc attttatttt taaattttta tttattttta tttttattta tttttgagac 3841 ggagtctcat tctgtcgcgg aggctgaagt gcagtggtgc gatctcagct cagtgcgacc 3901 tccgcctccc gggttcaagt gattctcctg cctcagcctc cggactagct gggattacag 3961 gcgcccgcct ccacgcccag ctaatttttg tatttttagt agagatgggg tttcgccatg 4021 ttggccaggc tggtctcgta ctcctgacct tgagtgatcc acctgcctcg gcctcccaaa 4081 gtgctgggat tacaggcatg agccaccgcg ccgggctaat atgctcattt tagtgaggca 4141 aaaatagagg ctcagagtct gatttgtaca aaactacaga gcagttaagt gtcctctcag 4201 atgtgtaccc tgatctgggt gactctagga ctctaggtct caactgttac aaccagttaa 4261 gggtttgggg aagcactggg ccaagagtca ggaaaatgga agccacaggt agtgcaaggt 4321 cttgggaatg ggacgtctgg tccaaggatt cacgcgatga ctggaacccg aagagccggg 4381 gcccggttta cggccgccat gaagcaacgc gcgccggtag gtttgggaat cagggagccc 4441 tctgaatagg agactgagtt gggagggaaa ggggcttcgc tgggggagcc tcggcttctt 4501 ctgggagaaa attcccacgg ctacctagtg agcctgcaaa ctggtaggcg ccggcgtagg 4561 cgcgcgggcg gggccggggg cggggcctgc ggggcgtggc ggggcgggca gagggcgggg 4621 cctgcttctc ctcagcttca ggcggctgcg acgagccctc aggcgaacct ctcggctttc 4681 ccgcgcggcg ccgcctcttg ctgcgcctcc gcctcctcct ctgctccgcc accggcttcc 4741 tcctcctgag cagtcagccc gcgcgccggc cggctccgtt atggcgaccc gcagccctgg 4801 cgtcgtggtg agcagctcgg cctgccggcc ctggccggtt caggcccacg cggcaggtgg 4861 cggccgggcc ctgaggcgcg ggatcgatcc atgacacccc cccgcccccc atatctcact 4921 ataattcact gtcaaatggc ttttatgatt tggagtttgg ggatactttt aaagacaggg 4981 tcttggtagc tcctagatgg cttgggattc acttagtaac ccttgctggc ctaaaattca 5041 ggaacttcta cctcatcttc ccaagtactg ggttagtagg gatgtccagc atatttagct 5101 tgctttaatg attttgctgt acttttttct gtgtgtacag gtgcatgtat atttataaaa 5161 ctaacataat atattttacc cccaaagtac cataacccta attaatgcta ttaaatgttt 5221 tgtatgctgg gctctgggca tactaatata accaatatta tacaatgcaa aacaattttg 5281 gaatatcttc tgtaaaatta tcttattagt aagttgtaac catctcagac ttctaagttt 5341 gagtttgtaa aggatcagtt ttttttggtg acctacacac acacacacac acacacacac 5401 acacacacac acaggggggt ggattctgag aatttagaga atttagatgg ttaataccag 5461 tttttatttt tattctgtgt gatatttatt tattaagatt tatttattta ttacatgtag 5521 gtacactgta tctgtcttca gacacaccag aagagggcat cagatctcgt tacagatggt 5581 tgtgagtcac catgtggttg ctgggatttg aactcaggac ctctggaaga acagtcagtg 5641 ctcttaaccg ttgagccatc tcaccagccc cgtgatattt tatttaactt actagaatat 5701 actgtgttaa aacatatttt aggggattgg ggagatggcc cattctgtaa agtgtctgcc 5761 atgtcatctt caggacctgc gtttgtaacc ccagcatgga gaagacagag acagggagac 5821 ccctgggctt cctggcaagc tagactagca aatttggtta actaggttca atgagagact 5881 cagtctcaaa acataagaag tgaatttttt agacccaatt tcaatctctg aactccatac 5941 gtatgcacat gtacctgcat ataaatatat atacacaccc ataaacacat gcacacacaa 6001 aaaatggttc aaaagttctt aaaatgtata agagtagaaa gtgtagagcc gggagtggtg 6061 gcgcacgcct ttaatcccag cacttgggag cagaggcagg tggatttctg agttcgaggc 6121 cagcctggtc tacagagtga gttccaggac agccagagct atacagagaa atcctgcctc 6181 aaaaaaccaa aaataaagtg tatatactaa tctttcatgc ctaaaatatt tatttttgcc 6241 acatttttca tacctcttta atatttttct ttttctcttt gtgcttcaaa ggaaattttg 6301 tgtcatgttt tacttttcat tcttgactct attcagcagt aagacgcagc atggctttct 6361 tagagaagta taatctatta caaaacggta acatttaact gctctacata ccctcttgta 6421 gctaaattcc tctgttaaac taagacttat tatctaaacg taattaaatg ttgatatctt 6481 ataatgctcc actttgaaac agctgtcttt cttttgcaga ttagcgatga tgaaccaggt 6541 tatgacctag atttgttttg tatacctaat cattatgccg aggatttgga aaaagtgttt 6601 attcctcatg gactgattat ggacaggtta gtaagacctc gattgaagtt atttttagag 6661 ggtgtgtgtg tggtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtaagagaga 6721 gaggagaggc atgtgtgata tatatgtgaa gtgtattgca tgtacatgga atccagagaa 6781 ggacattagg tgtcctactc tgttgctctc ttattccctt gaatcagtgt ctcttgctga 6841 acctggggca tacatactga tttggctagg ctgcctggct agcaagaccc agggatctgc 6901 ttgtctctgc cccccagtgc tgacgttaca agcaggtgca accacatctg gtttttatat 6961 gggtactggg ggatctgaac ttagggcctg atgcgtgccc aagtgctctc acccgccaag 7021 ccacctctcc agccaaaggg ttttttttct tgtattaatt ctaacttagc aactgttgag 7081 tagttgatgt ttgaatataa gctaagccaa cttattagaa taaatgtttt ctatttttta 7141 cttgtatgtg tgagtgcctg catgtatgta tgtgaagcat atgtattcca ggatgtcaga 7201 attgtgatgg attccctgga actggagtta cagaccattg tgaaccactg tgtatgggtg 7261 ctgggaactg aactttaggt cctctgcaaa aacagtaagg atcttaactg ataaactatc 7321 tttctagccc taaggctttc tacttttaaa gttcgtttct ctttctctct ctctctctct 7381 ctctctctct ctctctccct ctccctccct cctcctcctc ctcctctttc ccccctctcg 7441 cctcctcttt ctctctcccc tccctctctc tctctcacac acacacacag acagagagag 7501 gagagagaaa ggaagcctta ctaattaatt acctagtttt agacataact agtgggaatc 7561 catagtaagt aaatcaacat tcttgaagag aggttagaag aattggctct cgttgtccat 7621 cgaggctgta taagattatt cttctgtttt ctcagcttac catacatatt ttctacgtag 7681 acaataccaa ttcaaataca ctgtacgtaa agtatatgcg cacacacaca cacacacaca 7741 cacacacaca cacacaagtt acatattgtc atatagtact aaaatttatg gactttttca 7801 tattaataca tctagagcta tcatgttttt tatcttttaa aaattacttt acggggctac 7861 agagatgtct cagcagttag gagcactgac tgctcttcca gaggtcctga gttcaattcc 7921 cagcaaccac atggtggctc acaaccatct gtaatgggat ctgatccact cttatggtgt 7981 gtctgaagac agtgacagtg tactcatata catgaaataa atctttaaaa aattacttta 8041 ccatcagggt atatatatat atatatatgt gtgtgtgtgt gtgtgtgtgt atgaagtatt 8101 tgctatttga gagggtctca ctgtttagct cagtctggct tggaacgttc tgtatagccc 8161 agactggcct caactcagca gtccccctga aaggctctca tatgtggaga ttacaggtat 8221 gtgctactgt gcccagccct acatgttact ttgtttttga aatagggtat caaatagctt 8281 agactggctt ctaagtcagt gtgtagctaa ggatggtctg agcttctgaa cctgctactt 8341 caactcctgg tgtgccatca tggccagttt gcacagttgt gagagtagaa ctcaggacct 8401 cctatgtgtt actagacatg ttctctacca gctgagctac atcccaattc gcctacattt 8461 acttttattt tttaaaagat ttatttatgt atacagcact ctgcctgcat gtacatctgc 8521 atgccagaag agggcaccag atctcatcat acatagttgt gagccaccat gtggttgctg 8581 gggattgaac tcagggcctc tggaagagca gtcagtgctc ttaacctctg agccatctct 8641 gtagccccct acatttattt taaagattat aggctattct attcatagat gtgccataac 8701 tgatttaacc agatcttcat tattttgtac ttagtagagt atattagagg gaatgaatct 8761 taaatacaca ttttgacact tgctcagctt tagacaagta aaaatcactg gtcaaggaaa 8821 tggtgcttag tggatgcttc tgaactgtca aattgtcctc caacaagtta catcatttta 8881 tacttccaac gttatgtgag agttcatttt tctttaggct cttctagtat tttatatttg 8941 agaaataaaa tttaaccaaa atttgccaat ctagtaagct tgtttttatt tgtatcttta 9001 aattttatga tagatgttat agtgtactct cctctcccct cccctcccct ctcctcctca 9061 gcaggatctt gttatgtagc cctaagggat ctaatactta ctctgtagtc caggctgtcc 9121 ttcagctcat gagacacacc atcatgcttg gcatgtttct tgttctgaaa ggtatctggt 9181 tttaattttg gttgagctgg tgtgttggtg tatatctgta atctcagcac aggcaagtag 9241 agacaaggat tatgattaca gtccagcctg ggctatttag agggattctc tttaatcttt 9301 cctcatgccc caaaatctta cctttggtat atgaaaaata gtctccactt ctgcaaaata 9361 ttgctttatg aagtaagaat taattccctt catagagaca aggaatgtgt cctgtaaaag 9421 tttaatgtgt aagaagtatt tgttataaaa gataaatatt cagaatcttc tttttaattc 9481 ctgattttat ttctatagga ctgaaagact tgctcgagat gtcatgaagg agatgggagg 9541 ccatcacatt gtggccctct gtgtgctcaa ggggggctat aagttctttg ctgacctgct 9601 ggattacatt aaagcactga atagaaatag tgatagatcc attcctatga ctgtagattt 9661 tatcagactg aagagctact gtgtaagtat aattaactta taattaaaaa aatagggcca 9721 ttctagtttt atctatattt tttttaaact tgtgcaaact atgctacata atcaacttac 9781 ttgattatgt aatatatata gtaatagttg gaattactat tattacatat ttatttttct 9841 gtgctggggt agaacccagg gaactaagta ctgtaataac cgagcttcat tcatcctagt 9901 catttttaat tttttttttt ttttttgttt tttcgagaca aggtttctct gtgtagccct 9961 ggctgtcctg gaactcactc tgtagaccag gctggcctcg aactcagaaa tctgcctgcc 10021 tctgcctccc agtgctgaga ttaatgacgt gcaccaccac tgcctggcat ttttttaaag 10081 ttttaatttg agaccctttc tcactaactt gctcacacta tcctcgaact cattacgtgg 10141 gctggtctag aaatttcagt caacttgcct cagcttctca agtagctggg attacaggca 10201 ggtgctacca cttctggcca tgatcattgt tgttgttgct attattatta ttattatttc 10261 actgatactt catatcacaa cacagaatag taactgtagt tcatgaccaa caaaaaagtt 10321 gtacagtttc tctgttgtct tattttgatc tggattttga tttgtttcat ggcactgggc 10381 aggctaatct caaactgcat ggctcaaata gtccttctgt cttaatctcc catgttctgg 10441 aagtataggt atcactacac ctagctgtat tttcatattt taaaaaaaac gcctaagaat 10501 tgctattgaa tgaggcggag agatggctca gcagtcacaa gtacttgtag aggactgggg 10561 tttggttact agcacctcaa agatggctta caattatctg tagctccaat tccagaggat 10621 ctgtgtggta aagctgcatg tatatgggca tgcaggtaaa actgctaaac atataaaaat 10681 agctcagtct taagagaatt ggtcgacttt ttcgcccttt gacgttggag tccacgttct 10741 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 10801 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 10861 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg 10921 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 10981 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 11041 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 11101 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 11161 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 11221 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 11281 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 11341 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 11401 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 11461 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 11521 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 11581 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 11641 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 11701 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 11761 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 11821 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 11881 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 11941 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 12001 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 12061 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 12121 gctaccaggt cgagtaggga taacagggta atggatcctc gttgggcacc tgcactcatg 12181 tacgcaggcc cacatataga tgtacacacg catatcattc aaaatagaaa ttatttatac 12241 aagtagtgcg ttggagagct ggctcaggag ttgttaatgc ttgctaccct tgcaaaggac 12301 ccaagttcaa tttccagcac ctgtgttggg catctcacaa ccacatgtaa atctagtttc 12361 aggggatttc acccctcttc tgaactcttc aggcacttat acccacaaat ggacatctcc 12421 acatagagac acatactttt aaaaaagtaa tctagggtct agagaaatgg ttatagacca 12481 cggactggga tcagttccca gaagccacat cctgtggctc acaattgcct gtgaatcaag 12541 ttctagatct ggtggcctcg tctagcctgc agtcatcaac aaacacatgc acacatccat 12601 acacacataa tttaaaagaa agcgaaagta acctataatg ctaaacaaag cacattctca 12661 gtgtagactg cagtggtgat gaaccttgaa aatatattaa tagaagccag acacgtaagt 12721 cacaaatggt ataactcaat ttatatgaaa ttcccagaga gtccaaagtt aaaaaaaaca 12781 aaaaggcaga ttattggttg tcagtagcta cagagatggg acattgaaga tattaatagg 12841 tatagggttg aggattcatg gcctttccct cttccatgtt agcatgtcta ttgttgttaa 12901 ccttattcaa ctcatgttag ggcaatcata ttgatgagac ttaatgagta tagcttgaca 12961 ttcctagaag acagagtctc acatcagact ctctgatcct ctgactctta gaatctttca 13021 acaccctctt ctgaaatgat ctctgagcct ttggttgtga ctgaattatg agaaagaatt 13081 ttatgttgaa tgaattttac ttcagcattt ttacaaaact gaagctgggg atggcgtttc 13141 atgcgtgcag tccctaccat ttgcctataa gcctggagga ggcagaagta gaattaggct 13201 tttcagggac atcttcagct gcatcatgag cttgaggaca acttgagcta cataagattc 13261 ccatccccac ccccacacac acacacacat ctagtatgta cctagtatgc agtcttttca 13321 tatgtttaaa atgtagaaag gtatatgtga agtctccctt ttccccacaa cttagtccct 13381 ccaaagtatt gcatcagttt ccttgtacat gattttatgc atatataaga tacactattt 13441 ctctctctct ctctctcctt ccctccttcc ctccaacctc tgggtatgag ttgagggtta 13501 tgtatgtttc aagttgtaat aagaattgcc actcaaacat ttcatcccat tttaagttaa 13561 gtttgtctga ttttccactt tagaagggca gccatgatgg ctcatgccta taatcccatc 13621 ctagcacttg taagcctaat acataagact ggaggattgt catcagcttg aggctagcct 13681 ggtctaccta atgagttcca gccagccaag agtagaatat aagaccctgt ctccaaaaaa 13741 tgtaccaaag gggctggagg ggtaactcag tggttaagaa cagtagctgc tcttccagag 13801 gacctgggtt caattcccag cacccacatg gcagcttgca actatctgta actgtccagc 13861 tttgggatat ccgacaccat tataccaatt acataaaatg aaaaaattgt aaaatgtgct 13921 aaaatgagtg gggaggggga gcatggtgtg gttctgtggg cttgggattt tgccatgaat 13981 ccatgactat atctcagcaa tatgtggatt atttctggaa acctgtgggg catgggtgta 14041 tgggaagagg ggagagagag agagcccgca tccagccaga gttcctgtgc tctgggcagg 14101 cggacgtggg aggactgctg gacgatttcc acttggccct gggtgggcat ctggctgtgt 14161 gaagccactg accccactca gcggggggta gataaggggc atttcccata ccaggttctc 14221 agactccagc attccacgct ggatacagca gaagagctga gaacagaata ggggccccag 14281 ggagacactc tcgtcccaga gataaagaag gcagagggag agagattcct acgcaggcga 14341 gaggccttag tctgggcctt gatgaacaga gacggtctat ggttttagag ctttattgta 14401 gaaaggcagg gggaaagaga gaaggtagaa agagagagac cagccatggc caagaggaga 14461 gaagggggga aaggagagag agaagaaagg ctagagagta agaaaggtta aagagacaga 14521 agtaaaagct tagagtaaga gagagagaga gagcaagagg gagagtaaga aaagtaaaag 14581 cttaagagag cgaggagggg ccagcccctc taatagtggg cttgttatct tgttgttgct 14641 aggtaactgt gaagcgtcta gcctgaaggc cagaagcttg ggacattgtc tatgtgactg 14701 aaagccatga gcctctactg tgggggctgt ggaggtggta acttggacag gagccagggg 14761 ttccaggaga catgagggaa tgccttctgt cccatgtagg taggaattac caccaccggc 14821 tttcaccgta gttcactgga gactgtttgt acatagccca ttgccccaca gaaaccaggc 14881 actcactgtt tcatttgcat aagaattaga aggctttatt ttggctggga ttggtgactt 14941 gccccagaaa ttccagcttt tgggaagccg aatcctggag aaccaggagt tcaaggtcat 15001 cctcaggtac ctcatttgtt caagaccagc ctgtgctacc atgagacctt gtccctactc 15061 ccctcaaccc caaaacacgc tgttttcatt ttataggttg tactaaccat ggaggatggt 15121 catttgcctt tctcttgatt tcagaagcgt agcgcgggct ttgcaccatg atctagtgtt 15181 tccagatagg cttgcctctc cacagccagg cttatcctgc ttcagtccca tgtttggcaa 15241 gggcaacata tgactgaaga acttgcatag ccttatggtg tatcatgact cttagcccca 15301 gtttataact gtctaacagc tggtggcttt tgtgaaaaag gaaagcatca tttgaacttt 15361 taacttttca gctgtttgtt ttgactttgc ataaatactg agactagcta ggaaatgagc 15421 ttttgtttca tttgctaaaa atgatttttt tttcatgagg caatttcaag caatttgaat 15481 ttagcaatct ctacttgtct gaaaagaagt tattaattaa aaaaaaacaa acaaacagga 15541 acctattatg ctggctagtc acatgaaaga atccaaatga acacagtcaa ggctgaatca 15601 acacatgaaa ttattttcta attccccaaa ttacatttat gctaattagt atgtagtaaa 15661 acattttaat tgtttccaat gttaaacatt acagaaataa ttttgatgtt ttcctaataa 15721 cccagccttt gatcatgttt gcatttattt gattgttgtc agtttattaa ataattaagt 15781 aagagcaaca agataaaatg acttcatgag agtccttttc tgtaacttag aacgtcagta 15841 gtcataggaa ctgcggtcgt tcccttacct ttcagaagta ggctaaagag ttgaacgcaa 15901 aggtggtttt tcgaatcttc ctgatcctta agcccccact aggtttatat tctatatttg 15961 atatcggatt cgaacccctc gagcttagga tccggaaccc ttaatataac taagttatta 16021 ggtccctcga agaggttcac tagtactggc cattgcggcc gcttcgaggc tgcctcgcgc 16081 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 16141 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 16201 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa 16261 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 16321 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc 16381 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 16441 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 16501 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 16561 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 16621 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 16681 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 16741 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 16801 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 16861 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 16921 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 16981 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 17041 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 17101 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 17161 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 17221 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 17281 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 17341 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 17401 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 17461 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 17521 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 17581 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt 17641 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 17701 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 17761 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 17821 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 17881 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag 17941 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 18001 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 18061 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 18121 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 18181 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 18241 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg g //