LOCUS Ple49\(pEMS1360) 17204 bp DNA circular 17-NOV-2008 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2364 - 5' - GCGTATCACGAGGCCCTTTC- 3' Tm=56.0 Deg C Antisense primer: oEMS2468 - 5' - ATTGCTGGGGACAGACGGAG- 3' Tm=56.2 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 393bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|499191174| COMMENT VNTDBDATE|499191174| COMMENT LSOWNER| COMMENT VNTNAME|Ple49 (pEMS1360)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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14 (16 0) 1 2 0 0 Nil 991 100) COMMENT (CObjectList) (CObjectList)) COMMENT #194=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -395 100) COMMENT (CObjectList) (CObjectList)) COMMENT #195=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -186 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #196=(AnlzFontBackColorItem 138 404 50318387) COMMENT #197=(AnlzFontBackColorItem 632 893 41976063) COMMENT #198=(AnlzFontBackColorItem 53 72 43633766) COMMENT #199=(AnlzFontBackColorItem 425 631 50318387) COMMENT #200=(AnlzFontBackColorItem 405 424 43633766))) (CObList) 17498130 COMMENT (CStringList) 0 327734 (CObList))) FEATURES Location/Qualifiers misc_recomb 11067..14878 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker complement(15977..17198) /vntifkey="22" /label=AmpR misc_marker 9616..11041 /vntifkey="22" /label=AmpR misc_recomb 5975..9620 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 2021..5974 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 11048..11065 /vntifkey="21" /label=I-Sce\I misc_feature 903..936 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 937..947 /vntifkey="21" /label=Kozak\consensus misc_feature 944..1663 /vntifkey="21" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 1664..1699 /vntifkey="21" /label=Tetracysteine\residue misc_feature 1700..1772 /vntifkey="21" /label=NLS misc_feature 1778..1811 /vntifkey="21" /label=MFRT misc_feature 1965..2015 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..894 /vntifkey="21" /label=Ple49\(DBH-B) misc_feature 139..632 /vntifkey="21" /label=Region\1 misc_feature 633..894 /vntifkey="21" /label=Prom\region primer 54..73 /vntifkey="27" /label=oEMS2364 primer 406..425 /vntifkey="27" /label=oEMS2468 BASE COUNT 4582 a 3887 c 3896 g 4839 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccag ggagaggtgc cgtggtgaga ctgaccctcg ggcccacggg 181 tggatgatgg caaaggtcct gtggcaggtg tgggagaagc agggctgcgg ccagctgccc 241 tgagaggctt caaattcagg ccagatctgc tctgggcaag aggaagctgt gggtttggga 301 gcttcagaga caggggagca ggcctgtcac tcaccctctt ttccttaaag gctgtcaccc 361 ccagcagtgc accctgcagc ctgcctatcc cctgtgcagc tccagctccg tctgtcccca 421 gcaatgcacc ccacgcccat gtcccccact ccctatgatg ctctcgcgcc ttctggagca 481 gcagtggtac cagctggagg ctgtggaaaa attgagagga gacaaaagtg ggaagagcag 541 gccgtggaga gagtgtctaa acacagggac tatttggaga ttgtcttatt tttggtttga 601 aatgggccca cctgctagta ttgtacaaac gggctcttca gagccatctc agaagggaca 661 gcatccgcct gtctacttca actcccactg atgacgtcca tgtgtcatta gtgccaatta 721 gaggagggca gcaggctgag tgcttggcct ggggcgcaag cttgtgggag ggaaaattgg 781 attccccgct agacaaatgt gattacccgt gctgcctgga cccaccccat tcaggaccag 841 ggcataaatg gccaggtggg accagagagc tcaccccagc catgcccgcc ctcaggcgcg 901 ccgaagttcc tattcttcaa aaggtatagg aacttccgcc accatggtga gcaagggcga 961 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 1021 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 1081 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 1141 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 1201 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 1261 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 1321 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 1381 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 1441 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 1501 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 1561 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 1621 cgccgccggg atcactctcg gcatggacga gctgtacaag ttatttctga actgctgccc 1681 gggctgctgc atggaaccgg atccaaaaaa gaagagaaag gtagatccaa aaaagaagag 1741 aaaggtagat ccaaaaaaga agagaaaggt aatataagaa gttcctattc tctagaaagt 1801 ataggaactt cagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt 1861 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 1921 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 1981 cacaaatttc acaaataaag catttttttc actgcgaatt ctcgtaaaac tcttcatggc 2041 agtagttatt attctctctc tctctctttt tcttttttct tgagacagga tatttctctg 2101 ttgcccaggc tggagtgcag tggcacagtc ttggctcact gcagcctgga cctcctgggc 2161 tcaagccatc ctcccacctc agcctcccaa gtagctgggg ctacaggcac atggccacca 2221 ggcccagata atttttcatt tttgtagaga ctgagtctca ccatgttacc caggtttatt 2281 attctcattt tttagatgaa gagactgagg tccagagaag ctcaatgact tgcctagttt 2341 tacaaatctc ctgccatcac atacccctca gcgtccttaa taagagggag gccaccaact 2401 atgtgctggg cactgtggtg gatgctggag ctatagggtt gagtatataa gaaatggtgt 2461 tgctggagca actgttgctt gcttacctga cctatctgag aattaattag caggggaaca 2521 tatttttgtt ttcagattca atataagaac ttgtgtgggc aaaaataaag atcagtagta 2581 ataacagtag ttcccatttg ctgactgtac tgtcctaagt gcatatatat atacatacac 2641 acacgcatac ctatactcct ctaatactca aaatgatcct gtttatgtat tgttaatatg 2701 ctcattttat ttttaaattt ttatttattt ttatttttat ttatttttga gacggagtct 2761 cattctgtcg cggaggctga agtgcagtgg tgcgatctca gctcagtgcg acctccgcct 2821 cccgggttca agtgattctc ctgcctcagc ctccggacta gctgggatta caggcgcccg 2881 cctccacgcc cagctaattt ttgtattttt agtagagatg gggtttcgcc atgttggcca 2941 ggctggtctc gtactcctga ccttgagtga tccacctgcc tcggcctccc aaagtgctgg 3001 gattacaggc atgagccacc gcgccgggct aatatgctca ttttagtgag gcaaaaatag 3061 aggctcagag tctgatttgt acaaaactac agagcagtta agtgtcctct cagatgtgta 3121 ccctgatctg ggtgactcta ggactctagg tctcaactgt tacaaccagt taagggtttg 3181 gggaagcact gggccaagag tcaggaaaat ggaagccaca ggtagtgcaa ggtcttggga 3241 atgggacgtc tggtccaagg attcacgcga tgactggaac ccgaagagcc ggggcccggt 3301 ttacggccgc catgaagcaa cgcgcgccgg taggtttggg aatcagggag ccctctgaat 3361 aggagactga gttgggaggg aaaggggctt cgctggggga gcctcggctt cttctgggag 3421 aaaattccca cggctaccta gtgagcctgc aaactggtag gcgccggcgt aggcgcgcgg 3481 gcggggccgg gggcggggcc tgcggggcgt ggcggggcgg gcagagggcg gggcctgctt 3541 ctcctcagct tcaggcggct gcgacgagcc ctcaggcgaa cctctcggct ttcccgcgcg 3601 gcgccgcctc ttgctgcgcc tccgcctcct cctctgctcc gccaccggct tcctcctcct 3661 gagcagtcag cccgcgcgcc ggccggctcc gttatggcga cccgcagccc tggcgtcgtg 3721 gtgagcagct cggcctgccg gccctggccg gttcaggccc acgcggcagg tggcggccgg 3781 gccctgaggc gcgggatcga tccatgacac ccccccgccc cccatatctc actataattc 3841 actgtcaaat ggcttttatg atttggagtt tggggatact tttaaagaca gggtcttggt 3901 agctcctaga tggcttggga ttcacttagt aacccttgct ggcctaaaat tcaggaactt 3961 ctacctcatc ttcccaagta ctgggttagt agggatgtcc agcatattta gcttgcttta 4021 atgattttgc tgtacttttt tctgtgtgta caggtgcatg tatatttata aaactaacat 4081 aatatatttt acccccaaag taccataacc ctaattaatg ctattaaatg ttttgtatgc 4141 tgggctctgg gcatactaat ataaccaata ttatacaatg caaaacaatt ttggaatatc 4201 ttctgtaaaa ttatcttatt agtaagttgt aaccatctca gacttctaag tttgagtttg 4261 taaaggatca gttttttttg gtgacctaca cacacacaca cacacacaca cacacacaca 4321 cacacagggg ggtggattct gagaatttag agaatttaga tggttaatac cagtttttat 4381 ttttattctg tgtgatattt atttattaag atttatttat ttattacatg taggtacact 4441 gtatctgtct tcagacacac cagaagaggg catcagatct cgttacagat ggttgtgagt 4501 caccatgtgg ttgctgggat ttgaactcag gacctctgga agaacagtca gtgctcttaa 4561 ccgttgagcc atctcaccag ccccgtgata ttttatttaa cttactagaa tatactgtgt 4621 taaaacatat tttaggggat tggggagatg gcccattctg taaagtgtct gccatgtcat 4681 cttcaggacc tgcgtttgta accccagcat ggagaagaca gagacaggga gacccctggg 4741 cttcctggca agctagacta gcaaatttgg ttaactaggt tcaatgagag actcagtctc 4801 aaaacataag aagtgaattt tttagaccca atttcaatct ctgaactcca tacgtatgca 4861 catgtacctg catataaata tatatacaca cccataaaca catgcacaca caaaaaatgg 4921 ttcaaaagtt cttaaaatgt ataagagtag aaagtgtaga gccgggagtg gtggcgcacg 4981 cctttaatcc cagcacttgg gagcagaggc aggtggattt ctgagttcga ggccagcctg 5041 gtctacagag tgagttccag gacagccaga gctatacaga gaaatcctgc ctcaaaaaac 5101 caaaaataaa gtgtatatac taatctttca tgcctaaaat atttattttt gccacatttt 5161 tcatacctct ttaatatttt tctttttctc tttgtgcttc aaaggaaatt ttgtgtcatg 5221 ttttactttt cattcttgac tctattcagc agtaagacgc agcatggctt tcttagagaa 5281 gtataatcta ttacaaaacg gtaacattta actgctctac ataccctctt gtagctaaat 5341 tcctctgtta aactaagact tattatctaa acgtaattaa atgttgatat cttataatgc 5401 tccactttga aacagctgtc tttcttttgc agattagcga tgatgaacca ggttatgacc 5461 tagatttgtt ttgtatacct aatcattatg ccgaggattt ggaaaaagtg tttattcctc 5521 atggactgat tatggacagg ttagtaagac ctcgattgaa gttattttta gagggtgtgt 5581 gtgtggtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtaagag agagaggaga 5641 ggcatgtgtg atatatatgt gaagtgtatt gcatgtacat ggaatccaga gaaggacatt 5701 aggtgtccta ctctgttgct ctcttattcc cttgaatcag tgtctcttgc tgaacctggg 5761 gcatacatac tgatttggct aggctgcctg gctagcaaga cccagggatc tgcttgtctc 5821 tgccccccag tgctgacgtt acaagcaggt gcaaccacat ctggttttta tatgggtact 5881 gggggatctg aacttagggc ctgatgcgtg cccaagtgct ctcacccgcc aagccacctc 5941 tccagccaaa gggttttttt tcttgtatta attctaactt agcaactgtt gagtagttga 6001 tgtttgaata taagctaagc caacttatta gaataaatgt tttctatttt ttacttgtat 6061 gtgtgagtgc ctgcatgtat gtatgtgaag catatgtatt ccaggatgtc agaattgtga 6121 tggattccct ggaactggag ttacagacca ttgtgaacca ctgtgtatgg gtgctgggaa 6181 ctgaacttta ggtcctctgc aaaaacagta aggatcttaa ctgataaact atctttctag 6241 ccctaaggct ttctactttt aaagttcgtt tctctttctc tctctctctc tctctctctc 6301 tctctctctc cctctccctc cctcctcctc ctcctcctct ttcccccctc tcgcctcctc 6361 tttctctctc ccctccctct ctctctctca cacacacaca cagacagaga gaggagagag 6421 aaaggaagcc ttactaatta attacctagt tttagacata actagtggga atccatagta 6481 agtaaatcaa cattcttgaa gagaggttag aagaattggc tctcgttgtc catcgaggct 6541 gtataagatt attcttctgt tttctcagct taccatacat attttctacg tagacaatac 6601 caattcaaat acactgtacg taaagtatat gcgcacacac acacacacac acacacacac 6661 acacacacaa gttacatatt gtcatatagt actaaaattt atggactttt tcatattaat 6721 acatctagag ctatcatgtt ttttatcttt taaaaattac tttacggggc tacagagatg 6781 tctcagcagt taggagcact gactgctctt ccagaggtcc tgagttcaat tcccagcaac 6841 cacatggtgg ctcacaacca tctgtaatgg gatctgatcc actcttatgg tgtgtctgaa 6901 gacagtgaca gtgtactcat atacatgaaa taaatcttta aaaaattact ttaccatcag 6961 ggtatatata tatatatata tgtgtgtgtg tgtgtgtgtg tgtatgaagt atttgctatt 7021 tgagagggtc tcactgttta gctcagtctg gcttggaacg ttctgtatag cccagactgg 7081 cctcaactca gcagtccccc tgaaaggctc tcatatgtgg agattacagg tatgtgctac 7141 tgtgcccagc cctacatgtt actttgtttt tgaaataggg tatcaaatag cttagactgg 7201 cttctaagtc agtgtgtagc taaggatggt ctgagcttct gaacctgcta cttcaactcc 7261 tggtgtgcca tcatggccag tttgcacagt tgtgagagta gaactcagga cctcctatgt 7321 gttactagac atgttctcta ccagctgagc tacatcccaa ttcgcctaca tttactttta 7381 ttttttaaaa gatttattta tgtatacagc actctgcctg catgtacatc tgcatgccag 7441 aagagggcac cagatctcat catacatagt tgtgagccac catgtggttg ctggggattg 7501 aactcagggc ctctggaaga gcagtcagtg ctcttaacct ctgagccatc tctgtagccc 7561 cctacattta ttttaaagat tataggctat tctattcata gatgtgccat aactgattta 7621 accagatctt cattattttg tacttagtag agtatattag agggaatgaa tcttaaatac 7681 acattttgac acttgctcag ctttagacaa gtaaaaatca ctggtcaagg aaatggtgct 7741 tagtggatgc ttctgaactg tcaaattgtc ctccaacaag ttacatcatt ttatacttcc 7801 aacgttatgt gagagttcat ttttctttag gctcttctag tattttatat ttgagaaata 7861 aaatttaacc aaaatttgcc aatctagtaa gcttgttttt atttgtatct ttaaatttta 7921 tgatagatgt tatagtgtac tctcctctcc cctcccctcc cctctcctcc tcagcaggat 7981 cttgttatgt agccctaagg gatctaatac ttactctgta gtccaggctg tccttcagct 8041 catgagacac accatcatgc ttggcatgtt tcttgttctg aaaggtatct ggttttaatt 8101 ttggttgagc tggtgtgttg gtgtatatct gtaatctcag cacaggcaag tagagacaag 8161 gattatgatt acagtccagc ctgggctatt tagagggatt ctctttaatc tttcctcatg 8221 ccccaaaatc ttacctttgg tatatgaaaa atagtctcca cttctgcaaa atattgcttt 8281 atgaagtaag aattaattcc cttcatagag acaaggaatg tgtcctgtaa aagtttaatg 8341 tgtaagaagt atttgttata aaagataaat attcagaatc ttctttttaa ttcctgattt 8401 tatttctata ggactgaaag acttgctcga gatgtcatga aggagatggg aggccatcac 8461 attgtggccc tctgtgtgct caaggggggc tataagttct ttgctgacct gctggattac 8521 attaaagcac tgaatagaaa tagtgataga tccattccta tgactgtaga ttttatcaga 8581 ctgaagagct actgtgtaag tataattaac ttataattaa aaaaataggg ccattctagt 8641 tttatctata ttttttttaa acttgtgcaa actatgctac ataatcaact tacttgatta 8701 tgtaatatat atagtaatag ttggaattac tattattaca tatttatttt tctgtgctgg 8761 ggtagaaccc agggaactaa gtactgtaat aaccgagctt cattcatcct agtcattttt 8821 aatttttttt tttttttttg ttttttcgag acaaggtttc tctgtgtagc cctggctgtc 8881 ctggaactca ctctgtagac caggctggcc tcgaactcag aaatctgcct gcctctgcct 8941 cccagtgctg agattaatga cgtgcaccac cactgcctgg cattttttta aagttttaat 9001 ttgagaccct ttctcactaa cttgctcaca ctatcctcga actcattacg tgggctggtc 9061 tagaaatttc agtcaacttg cctcagcttc tcaagtagct gggattacag gcaggtgcta 9121 ccacttctgg ccatgatcat tgttgttgtt gctattatta ttattattat ttcactgata 9181 cttcatatca caacacagaa tagtaactgt agttcatgac caacaaaaaa gttgtacagt 9241 ttctctgttg tcttattttg atctggattt tgatttgttt catggcactg ggcaggctaa 9301 tctcaaactg catggctcaa atagtccttc tgtcttaatc tcccatgttc tggaagtata 9361 ggtatcacta cacctagctg tattttcata ttttaaaaaa aacgcctaag aattgctatt 9421 gaatgaggcg gagagatggc tcagcagtca caagtacttg tagaggactg gggtttggtt 9481 actagcacct caaagatggc ttacaattat ctgtagctcc aattccagag gatctgtgtg 9541 gtaaagctgc atgtatatgg gcatgcaggt aaaactgcta aacatataaa aatagctcag 9601 tcttaagaga attggtcgac tttttcgccc tttgacgttg gagtccacgt tctttaatag 9661 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 9721 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 9781 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 9841 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 9901 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 9961 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 10021 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 10081 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 10141 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 10201 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 10261 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 10321 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 10381 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 10441 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 10501 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 10561 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 10621 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 10681 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 10741 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 10801 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 10861 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 10921 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 10981 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 11041 ggtcgagtag ggataacagg gtaatggatc ctcgttgggc acctgcactc atgtacgcag 11101 gcccacatat agatgtacac acgcatatca ttcaaaatag aaattattta tacaagtagt 11161 gcgttggaga gctggctcag gagttgttaa tgcttgctac ccttgcaaag gacccaagtt 11221 caatttccag cacctgtgtt gggcatctca caaccacatg taaatctagt ttcaggggat 11281 ttcacccctc ttctgaactc ttcaggcact tatacccaca aatggacatc tccacataga 11341 gacacatact tttaaaaaag taatctaggg tctagagaaa tggttataga ccacggactg 11401 ggatcagttc ccagaagcca catcctgtgg ctcacaattg cctgtgaatc aagttctaga 11461 tctggtggcc tcgtctagcc tgcagtcatc aacaaacaca tgcacacatc catacacaca 11521 taatttaaaa gaaagcgaaa gtaacctata atgctaaaca aagcacattc tcagtgtaga 11581 ctgcagtggt gatgaacctt gaaaatatat taatagaagc cagacacgta agtcacaaat 11641 ggtataactc aatttatatg aaattcccag agagtccaaa gttaaaaaaa acaaaaaggc 11701 agattattgg ttgtcagtag ctacagagat gggacattga agatattaat aggtataggg 11761 ttgaggattc atggcctttc cctcttccat gttagcatgt ctattgttgt taaccttatt 11821 caactcatgt tagggcaatc atattgatga gacttaatga gtatagcttg acattcctag 11881 aagacagagt ctcacatcag actctctgat cctctgactc ttagaatctt tcaacaccct 11941 cttctgaaat gatctctgag cctttggttg tgactgaatt atgagaaaga attttatgtt 12001 gaatgaattt tacttcagca tttttacaaa actgaagctg gggatggcgt ttcatgcgtg 12061 cagtccctac catttgccta taagcctgga ggaggcagaa gtagaattag gcttttcagg 12121 gacatcttca gctgcatcat gagcttgagg acaacttgag ctacataaga ttcccatccc 12181 cacccccaca cacacacaca catctagtat gtacctagta tgcagtcttt tcatatgttt 12241 aaaatgtaga aaggtatatg tgaagtctcc cttttcccca caacttagtc cctccaaagt 12301 attgcatcag tttccttgta catgatttta tgcatatata agatacacta tttctctctc 12361 tctctctctc cttccctcct tccctccaac ctctgggtat gagttgaggg ttatgtatgt 12421 ttcaagttgt aataagaatt gccactcaaa catttcatcc cattttaagt taagtttgtc 12481 tgattttcca ctttagaagg gcagccatga tggctcatgc ctataatccc atcctagcac 12541 ttgtaagcct aatacataag actggaggat tgtcatcagc ttgaggctag cctggtctac 12601 ctaatgagtt ccagccagcc aagagtagaa tataagaccc tgtctccaaa aaatgtacca 12661 aaggggctgg aggggtaact cagtggttaa gaacagtagc tgctcttcca gaggacctgg 12721 gttcaattcc cagcacccac atggcagctt gcaactatct gtaactgtcc agctttggga 12781 tatccgacac cattatacca attacataaa atgaaaaaat tgtaaaatgt gctaaaatga 12841 gtggggaggg ggagcatggt gtggttctgt gggcttggga ttttgccatg aatccatgac 12901 tatatctcag caatatgtgg attatttctg gaaacctgtg gggcatgggt gtatgggaag 12961 aggggagaga gagagagccc gcatccagcc agagttcctg tgctctgggc aggcggacgt 13021 gggaggactg ctggacgatt tccacttggc cctgggtggg catctggctg tgtgaagcca 13081 ctgaccccac tcagcggggg gtagataagg ggcatttccc ataccaggtt ctcagactcc 13141 agcattccac gctggataca gcagaagagc tgagaacaga ataggggccc cagggagaca 13201 ctctcgtccc agagataaag aaggcagagg gagagagatt cctacgcagg cgagaggcct 13261 tagtctgggc cttgatgaac agagacggtc tatggtttta gagctttatt gtagaaaggc 13321 agggggaaag agagaaggta gaaagagaga gaccagccat ggccaagagg agagaagggg 13381 ggaaaggaga gagagaagaa aggctagaga gtaagaaagg ttaaagagac agaagtaaaa 13441 gcttagagta agagagagag agagagcaag agggagagta agaaaagtaa aagcttaaga 13501 gagcgaggag gggccagccc ctctaatagt gggcttgtta tcttgttgtt gctaggtaac 13561 tgtgaagcgt ctagcctgaa ggccagaagc ttgggacatt gtctatgtga ctgaaagcca 13621 tgagcctcta ctgtgggggc tgtggaggtg gtaacttgga caggagccag gggttccagg 13681 agacatgagg gaatgccttc tgtcccatgt aggtaggaat taccaccacc ggctttcacc 13741 gtagttcact ggagactgtt tgtacatagc ccattgcccc acagaaacca ggcactcact 13801 gtttcatttg cataagaatt agaaggcttt attttggctg ggattggtga cttgccccag 13861 aaattccagc ttttgggaag ccgaatcctg gagaaccagg agttcaaggt catcctcagg 13921 tacctcattt gttcaagacc agcctgtgct accatgagac cttgtcccta ctcccctcaa 13981 ccccaaaaca cgctgttttc attttatagg ttgtactaac catggaggat ggtcatttgc 14041 ctttctcttg atttcagaag cgtagcgcgg gctttgcacc atgatctagt gtttccagat 14101 aggcttgcct ctccacagcc aggcttatcc tgcttcagtc ccatgtttgg caagggcaac 14161 atatgactga agaacttgca tagccttatg gtgtatcatg actcttagcc ccagtttata 14221 actgtctaac agctggtggc ttttgtgaaa aaggaaagca tcatttgaac ttttaacttt 14281 tcagctgttt gttttgactt tgcataaata ctgagactag ctaggaaatg agcttttgtt 14341 tcatttgcta aaaatgattt ttttttcatg aggcaatttc aagcaatttg aatttagcaa 14401 tctctacttg tctgaaaaga agttattaat taaaaaaaaa caaacaaaca ggaacctatt 14461 atgctggcta gtcacatgaa agaatccaaa tgaacacagt caaggctgaa tcaacacatg 14521 aaattatttt ctaattcccc aaattacatt tatgctaatt agtatgtagt aaaacatttt 14581 aattgtttcc aatgttaaac attacagaaa taattttgat gttttcctaa taacccagcc 14641 tttgatcatg tttgcattta tttgattgtt gtcagtttat taaataatta agtaagagca 14701 acaagataaa atgacttcat gagagtcctt ttctgtaact tagaacgtca gtagtcatag 14761 gaactgcggt cgttccctta cctttcagaa gtaggctaaa gagttgaacg caaaggtggt 14821 ttttcgaatc ttcctgatcc ttaagccccc actaggttta tattctatat ttgatatcgg 14881 attcgaaccc ctcgagctta ggatccggaa cccttaatat aactaagtta ttaggtccct 14941 cgaagaggtt cactagtact ggccattgcg gccgcttcga ggctgcctcg cgcgtttcgg 15001 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 15061 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 15121 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg 15181 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc 15241 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc 15301 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 15361 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 15421 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 15481 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 15541 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 15601 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 15661 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 15721 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 15781 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 15841 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 15901 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 15961 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 16021 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 16081 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 16141 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 16201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 16261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 16321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 16381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 16441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 16501 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg 16561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 16621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 16681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 16741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 16801 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta 16861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 16921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 16981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 17041 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 17101 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 17161 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgg //