LOCUS Ple54\(pEMS1200) 20135 bp DNA circular 26-JAN-2009 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2754 5'- CTTCAAATCACAGCCTTTTTACGC -3' Tm= 56.2 Deg C Antisense primer: oEMS2749 5'- CTCTAATGAGCAATGTGCTAGCCC -3' Tm=56.2 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 546 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|508255245| COMMENT VNTDBDATE|508255245| COMMENT LSOWNER| COMMENT VNTNAME|Ple54 (pEMS1200)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#207=(AnlzFontBackColorItem 1769 1792 43633766))) (CObList) 17498167 COMMENT (CStringList) 0 851972 (CObList))) FEATURES Location/Qualifiers misc_recomb 13998..17809 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(18908..20129) /vntifkey="22" /label=AmpR misc_marker 12547..13972 /vntifkey="22" /label=AmpR misc_recomb 8906..12551 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4952..8905 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 13979..13996 /vntifkey="21" /label=I-Sce\I misc_feature 3834..3867 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 3868..3878 /vntifkey="21" /label=Kozak\consensus misc_feature 3875..4594 /vntifkey="21" /label=EGFP misc_feature 4595..4630 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4631..4703 /vntifkey="21" /label=NLS misc_feature 4709..4742 /vntifkey="21" /label=MFRT misc_feature 4896..4946 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..3825 /vntifkey="21" /label=Ple54\(DCX-C) /note="1306" misc_feature 139..775 /vntifkey="21" /label=Region\1 misc_feature 776..1454 /vntifkey="21" /label=Region2 misc_feature 1455..3825 /vntifkey="21" /label=Prom\region primer 1248..1271 /vntifkey="27" /label=oEMS2754 primer 1770..1793 /vntifkey="27" /label=oEMS2749 BASE COUNT 5372 a 4471 c 4476 g 5816 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggcctg tgactgaaaa tgatatttgc ccattcatta ctttttagat 181 accctcacat tgatgctcag catctatttt gataaaagga agttgaagtg accttcatat 241 gctctgaaag aagtcagata cggggaaagc actgaagcct ctggctcgtt gtctttgcct 301 cacactaaac catatttatt ctctttctct tttccatatg acatagctgt cttaatttgg 361 ttccactgat ggtttgtgtg agaaagggac tatttcagga tcctaaaatt agcccaagaa 421 agatgctgcc atctgttccc cctccaaatc aggttcttca tgtcattggt tatactacta 481 aagtgccacc tatattaata tctgggaaag aatgcagcca ggcgtctcca tcagacagat 541 gcaccattct cttaagcata gggagctgta aatatacaac aaaaatgttc caagggaatt 601 ggaacagtgg ctggttctgt tggctctaaa gagaattctg gtctcccatc agaaaagaca 661 agtgtggatt agtatcagtc catatattct acctcttaaa atgcctttgg tgtttaaaca 721 actatataga aaaggtattc ctaaaaggcg gattcactat atatcaaagt tactgtccca 781 ctcctccttc aaccctctca ttttcacacg taggagtggt aggtagagca gatgcaggtg 841 acagaaagct gaacccaagc agtccgattg ggaaattgat tctcaacagg aagggacttc 901 gatctctgct tatcttttct cactcaaatg cggcgttaga ccccaagaca aagctcagat 961 tgatgcagtc cccaccccac ccttctcctg gcttcttttc tgtaagtaat gctttcagtg 1021 tttggagtct ggcagctgca aggcctggtt gctaggtaac aatttctcag cacaaagaac 1081 cttcagctgg gaaggtcagg tctatcagtg tgaaacagct gtaaagaatt tttatttgtc 1141 tcctcttgtt ttggttaatc acagggcagg gaaccaaaat caaactttcc atagaaatga 1201 gagataccgg agggtaccag tttagctgct gctgattttt tgcagctctt caaatcacag 1261 cctttttacg ctggctcaat agagccccaa caagcttgtc aggctggaag tggaggcagg 1321 aggaggaggg gtcaggtgtt tcctagcctc tttagtgcct caggcaggaa ggacatagca 1381 aaaacatgag ctgagaagtt aagtcctttt cttcaaattt accagcagtt tcttcagatt 1441 agcgttgtca gatttgatag atttgggatg tcctttttac ttctatcttc cataatattc 1501 taaaattatt ttcccttgtt ttgttcctat cctacttcct cttagtctac tttgttgact 1561 tcattaaaaa acaaaaaacc agttgttgga tacttgagct aaactgcctt aaagaatctg 1621 cagattttat tttatttttt ttctctcaag agggtaaaag gaagagagct acaatttcta 1681 agaagcctgg cttggctgtc tgagtctggc ccccaggcag attaggccaa ggttttggcc 1741 aagtgaaatt gccaattttc taaaagaaag ggctagcaca ttgctcatta gagcattctg 1801 attttgtctg cgcaatcttt ctgctacccc gcaatttcct gttggttata aatgaaacct 1861 ttctagctgt taatgcagcc tgtgaatttt tttaaaagca tgtaattaat cataggaggt 1921 tggggggatt cactaagcct gagttacatg ggagaagctg gacaaggcac taggacctag 1981 aaggcatcta tccaccctgg caggaatttc ttgcttggag ctcagacaac aaaggcatag 2041 agagattggt tttctttctc tcagcatctc cacccaacca gcagaaaacc ggtgagtggg 2101 gcttttaagt gattttcaag aagaatgtaa cagatgtcaa acgggaaaag cacgaggcaa 2161 agcctgctct ctctgtctct ctgtctcctc ttctcctttt ttgccttatt ctatccgatt 2221 ttttccctaa gcttctacct gggattttcc tttggaaaag tgagtttgat gttcctttgt 2281 tttcactgtg atgttaattt agaataatac tacctctgat cctaaagcaa agcaaagcct 2341 tactggcatg cctggggaaa tgtttgctgc ttgccttgag gaggtggggt ctcttaccac 2401 tgcaggttgt ctgacagaga caatgctgag ctcagcatag gtcatggtga cattggaaaa 2461 aaggcggaat tgagcctggc agacccatta ggcaccagtc tttcttatct cctgtcctcc 2521 tggtcccttg caaatatatt gatgtggcag tgtgtagcag ctgagccctg cttgctttgt 2581 gagtcctttt atccccatct gtgagatgca tgttaatagt ttggctcgta ggatgtcact 2641 acatttgcta gcatttgtgg cttcagttgt attgggtttc atgttttgat tgtttggggt 2701 tcttggtggg ggagggggtt caacagaagg gagaaaagca aagcctgaca aatgaccatc 2761 ttttctcagc taatgcacct gggcaatata caagtttggg gtgaattgcc tgctgtgagg 2821 gtaaatgtca cttcaattaa ggtagaaacc cagaacaatg aaaggtgtgc ttccttctaa 2881 aggtcccgta tgctgttcgg agagtcattt gtgaatcttt caacaattaa attattccat 2941 taagaggtgt tgctgcatca gtggggaggg ggtggagcac ctggggggga aaaaaaagga 3001 ttttgtgaac aaatggaacc gggggaagac agagctagta acttgttaaa taacttattt 3061 ttctaatcct ttttcccccc agcttatttc ttatgaatgt cggatagctg caccagcttg 3121 gtggggaaag ggtttgatga atagcacaaa gacactggct gttccctgga ggctgtccct 3181 ttaaaggaga atcttagttt attctggggg gaggggatgc acacattaga gtaggaaaga 3241 gggcttggaa taaaatgaaa acactccccc ttcatagtca ttgtactgaa atgcaaagac 3301 tgcttcctaa gctggagatg ctaaccttgg gtagctcctt ctgttctctt caaggggaat 3361 tttgtcaggc tatggattca tttacaactg ttagtcatgt gggcatgtgt gaggaaacag 3421 atgccagttt taatgtattt agcccgaagt tccaatttga taggagccac tgtcagtaag 3481 tctcaggatt ttcagctatt tcaaaatctc cccttctcct ctgtctggaa cagtgccaag 3541 agtgcctccc tctctatctc ttactcccaa cccccacaac caccagcacc cccgcccagc 3601 ccctccttct tctctattaa gatcaatatt cctgcaggtc aggggcaagc agcagatggg 3661 tcacaggctt ttttcaacca gttcttttca caagcagcag attgcagatc tggatctggc 3721 taatatttaa aatcccttct tttttccttc tccttgtccc tttttgtttt tgcctctctt 3781 cacccccatc cctttctccc acgctcaggt ctctgaggtt ccaccggcgc gccgaagttc 3841 ctattcttca aaaggtatag gaacttccgc caccatggtg agcaagggcg aggagctgtt 3901 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 3961 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 4021 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 4081 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 4141 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 4201 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 4261 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 4321 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 4381 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 4441 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 4501 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 4561 gatcactctc ggcatggacg agctgtacaa gttatttctg aactgctgcc cgggctgctg 4621 catggaaccg gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtaga 4681 tccaaaaaag aagagaaagg taatataaga agttcctatt ctctagaaag tataggaact 4741 tcagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc 4801 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 4861 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 4921 cacaaataaa gcattttttt cactgcgaat tctcgtaaaa ctcttcatgg cagtagttat 4981 tattctctct ctctctcttt ttcttttttc ttgagacagg atatttctct gttgcccagg 5041 ctggagtgca gtggcacagt cttggctcac tgcagcctgg acctcctggg ctcaagccat 5101 cctcccacct cagcctccca agtagctggg gctacaggca catggccacc aggcccagat 5161 aatttttcat ttttgtagag actgagtctc accatgttac ccaggtttat tattctcatt 5221 ttttagatga agagactgag gtccagagaa gctcaatgac ttgcctagtt ttacaaatct 5281 cctgccatca catacccctc agcgtcctta ataagaggga ggccaccaac tatgtgctgg 5341 gcactgtggt ggatgctgga gctatagggt tgagtatata agaaatggtg ttgctggagc 5401 aactgttgct tgcttacctg acctatctga gaattaatta gcaggggaac atatttttgt 5461 tttcagattc aatataagaa cttgtgtggg caaaaataaa gatcagtagt aataacagta 5521 gttcccattt gctgactgta ctgtcctaag tgcatatata tatacataca cacacgcata 5581 cctatactcc tctaatactc aaaatgatcc tgtttatgta ttgttaatat gctcatttta 5641 tttttaaatt tttatttatt tttattttta tttatttttg agacggagtc tcattctgtc 5701 gcggaggctg aagtgcagtg gtgcgatctc agctcagtgc gacctccgcc tcccgggttc 5761 aagtgattct cctgcctcag cctccggact agctgggatt acaggcgccc gcctccacgc 5821 ccagctaatt tttgtatttt tagtagagat ggggtttcgc catgttggcc aggctggtct 5881 cgtactcctg accttgagtg atccacctgc ctcggcctcc caaagtgctg ggattacagg 5941 catgagccac cgcgccgggc taatatgctc attttagtga ggcaaaaata gaggctcaga 6001 gtctgatttg tacaaaacta cagagcagtt aagtgtcctc tcagatgtgt accctgatct 6061 gggtgactct aggactctag gtctcaactg ttacaaccag ttaagggttt ggggaagcac 6121 tgggccaaga gtcaggaaaa tggaagccac aggtagtgca aggtcttggg aatgggacgt 6181 ctggtccaag gattcacgcg atgactggaa cccgaagagc cggggcccgg tttacggccg 6241 ccatgaagca acgcgcgccg gtaggtttgg gaatcaggga gccctctgaa taggagactg 6301 agttgggagg gaaaggggct tcgctggggg agcctcggct tcttctggga gaaaattccc 6361 acggctacct agtgagcctg caaactggta ggcgccggcg taggcgcgcg ggcggggccg 6421 ggggcggggc ctgcggggcg tggcggggcg ggcagagggc ggggcctgct tctcctcagc 6481 ttcaggcggc tgcgacgagc cctcaggcga acctctcggc tttcccgcgc ggcgccgcct 6541 cttgctgcgc ctccgcctcc tcctctgctc cgccaccggc ttcctcctcc tgagcagtca 6601 gcccgcgcgc cggccggctc cgttatggcg acccgcagcc ctggcgtcgt ggtgagcagc 6661 tcggcctgcc ggccctggcc ggttcaggcc cacgcggcag gtggcggccg ggccctgagg 6721 cgcgggatcg atccatgaca cccccccgcc ccccatatct cactataatt cactgtcaaa 6781 tggcttttat gatttggagt ttggggatac ttttaaagac agggtcttgg tagctcctag 6841 atggcttggg attcacttag taacccttgc tggcctaaaa ttcaggaact tctacctcat 6901 cttcccaagt actgggttag tagggatgtc cagcatattt agcttgcttt aatgattttg 6961 ctgtactttt ttctgtgtgt acaggtgcat gtatatttat aaaactaaca taatatattt 7021 tacccccaaa gtaccataac cctaattaat gctattaaat gttttgtatg ctgggctctg 7081 ggcatactaa tataaccaat attatacaat gcaaaacaat tttggaatat cttctgtaaa 7141 attatcttat tagtaagttg taaccatctc agacttctaa gtttgagttt gtaaaggatc 7201 agtttttttt ggtgacctac acacacacac acacacacac acacacacac acacacaggg 7261 gggtggattc tgagaattta gagaatttag atggttaata ccagttttta tttttattct 7321 gtgtgatatt tatttattaa gatttattta tttattacat gtaggtacac tgtatctgtc 7381 ttcagacaca ccagaagagg gcatcagatc tcgttacaga tggttgtgag tcaccatgtg 7441 gttgctggga tttgaactca ggacctctgg aagaacagtc agtgctctta accgttgagc 7501 catctcacca gccccgtgat attttattta acttactaga atatactgtg ttaaaacata 7561 ttttagggga ttggggagat ggcccattct gtaaagtgtc tgccatgtca tcttcaggac 7621 ctgcgtttgt aaccccagca tggagaagac agagacaggg agacccctgg gcttcctggc 7681 aagctagact agcaaatttg gttaactagg ttcaatgaga gactcagtct caaaacataa 7741 gaagtgaatt ttttagaccc aatttcaatc tctgaactcc atacgtatgc acatgtacct 7801 gcatataaat atatatacac acccataaac acatgcacac acaaaaaatg gttcaaaagt 7861 tcttaaaatg tataagagta gaaagtgtag agccgggagt ggtggcgcac gcctttaatc 7921 ccagcacttg ggagcagagg caggtggatt tctgagttcg aggccagcct ggtctacaga 7981 gtgagttcca ggacagccag agctatacag agaaatcctg cctcaaaaaa ccaaaaataa 8041 agtgtatata ctaatctttc atgcctaaaa tatttatttt tgccacattt ttcatacctc 8101 tttaatattt ttctttttct ctttgtgctt caaaggaaat tttgtgtcat gttttacttt 8161 tcattcttga ctctattcag cagtaagacg cagcatggct ttcttagaga agtataatct 8221 attacaaaac ggtaacattt aactgctcta cataccctct tgtagctaaa ttcctctgtt 8281 aaactaagac ttattatcta aacgtaatta aatgttgata tcttataatg ctccactttg 8341 aaacagctgt ctttcttttg cagattagcg atgatgaacc aggttatgac ctagatttgt 8401 tttgtatacc taatcattat gccgaggatt tggaaaaagt gtttattcct catggactga 8461 ttatggacag gttagtaaga cctcgattga agttattttt agagggtgtg tgtgtggtgt 8521 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtaaga gagagaggag aggcatgtgt 8581 gatatatatg tgaagtgtat tgcatgtaca tggaatccag agaaggacat taggtgtcct 8641 actctgttgc tctcttattc ccttgaatca gtgtctcttg ctgaacctgg ggcatacata 8701 ctgatttggc taggctgcct ggctagcaag acccagggat ctgcttgtct ctgcccccca 8761 gtgctgacgt tacaagcagg tgcaaccaca tctggttttt atatgggtac tgggggatct 8821 gaacttaggg cctgatgcgt gcccaagtgc tctcacccgc caagccacct ctccagccaa 8881 agggtttttt ttcttgtatt aattctaact tagcaactgt tgagtagttg atgtttgaat 8941 ataagctaag ccaacttatt agaataaatg ttttctattt tttacttgta tgtgtgagtg 9001 cctgcatgta tgtatgtgaa gcatatgtat tccaggatgt cagaattgtg atggattccc 9061 tggaactgga gttacagacc attgtgaacc actgtgtatg ggtgctggga actgaacttt 9121 aggtcctctg caaaaacagt aaggatctta actgataaac tatctttcta gccctaaggc 9181 tttctacttt taaagttcgt ttctctttct ctctctctct ctctctctct ctctctctct 9241 ccctctccct ccctcctcct cctcctcctc tttcccccct ctcgcctcct ctttctctct 9301 cccctccctc tctctctctc acacacacac acagacagag agaggagaga gaaaggaagc 9361 cttactaatt aattacctag ttttagacat aactagtggg aatccatagt aagtaaatca 9421 acattcttga agagaggtta gaagaattgg ctctcgttgt ccatcgaggc tgtataagat 9481 tattcttctg ttttctcagc ttaccataca tattttctac gtagacaata ccaattcaaa 9541 tacactgtac gtaaagtata tgcgcacaca cacacacaca cacacacaca cacacacaca 9601 agttacatat tgtcatatag tactaaaatt tatggacttt ttcatattaa tacatctaga 9661 gctatcatgt tttttatctt ttaaaaatta ctttacgggg ctacagagat gtctcagcag 9721 ttaggagcac tgactgctct tccagaggtc ctgagttcaa ttcccagcaa ccacatggtg 9781 gctcacaacc atctgtaatg ggatctgatc cactcttatg gtgtgtctga agacagtgac 9841 agtgtactca tatacatgaa ataaatcttt aaaaaattac tttaccatca gggtatatat 9901 atatatatat atgtgtgtgt gtgtgtgtgt gtgtatgaag tatttgctat ttgagagggt 9961 ctcactgttt agctcagtct ggcttggaac gttctgtata gcccagactg gcctcaactc 10021 agcagtcccc ctgaaaggct ctcatatgtg gagattacag gtatgtgcta ctgtgcccag 10081 ccctacatgt tactttgttt ttgaaatagg gtatcaaata gcttagactg gcttctaagt 10141 cagtgtgtag ctaaggatgg tctgagcttc tgaacctgct acttcaactc ctggtgtgcc 10201 atcatggcca gtttgcacag ttgtgagagt agaactcagg acctcctatg tgttactaga 10261 catgttctct accagctgag ctacatccca attcgcctac atttactttt attttttaaa 10321 agatttattt atgtatacag cactctgcct gcatgtacat ctgcatgcca gaagagggca 10381 ccagatctca tcatacatag ttgtgagcca ccatgtggtt gctggggatt gaactcaggg 10441 cctctggaag agcagtcagt gctcttaacc tctgagccat ctctgtagcc ccctacattt 10501 attttaaaga ttataggcta ttctattcat agatgtgcca taactgattt aaccagatct 10561 tcattatttt gtacttagta gagtatatta gagggaatga atcttaaata cacattttga 10621 cacttgctca gctttagaca agtaaaaatc actggtcaag gaaatggtgc ttagtggatg 10681 cttctgaact gtcaaattgt cctccaacaa gttacatcat tttatacttc caacgttatg 10741 tgagagttca tttttcttta ggctcttcta gtattttata tttgagaaat aaaatttaac 10801 caaaatttgc caatctagta agcttgtttt tatttgtatc tttaaatttt atgatagatg 10861 ttatagtgta ctctcctctc ccctcccctc ccctctcctc ctcagcagga tcttgttatg 10921 tagccctaag ggatctaata cttactctgt agtccaggct gtccttcagc tcatgagaca 10981 caccatcatg cttggcatgt ttcttgttct gaaaggtatc tggttttaat tttggttgag 11041 ctggtgtgtt ggtgtatatc tgtaatctca gcacaggcaa gtagagacaa ggattatgat 11101 tacagtccag cctgggctat ttagagggat tctctttaat ctttcctcat gccccaaaat 11161 cttacctttg gtatatgaaa aatagtctcc acttctgcaa aatattgctt tatgaagtaa 11221 gaattaattc ccttcataga gacaaggaat gtgtcctgta aaagtttaat gtgtaagaag 11281 tatttgttat aaaagataaa tattcagaat cttcttttta attcctgatt ttatttctat 11341 aggactgaaa gacttgctcg agatgtcatg aaggagatgg gaggccatca cattgtggcc 11401 ctctgtgtgc tcaagggggg ctataagttc tttgctgacc tgctggatta cattaaagca 11461 ctgaatagaa atagtgatag atccattcct atgactgtag attttatcag actgaagagc 11521 tactgtgtaa gtataattaa cttataatta aaaaaatagg gccattctag ttttatctat 11581 atttttttta aacttgtgca aactatgcta cataatcaac ttacttgatt atgtaatata 11641 tatagtaata gttggaatta ctattattac atatttattt ttctgtgctg gggtagaacc 11701 cagggaacta agtactgtaa taaccgagct tcattcatcc tagtcatttt taattttttt 11761 tttttttttt gttttttcga gacaaggttt ctctgtgtag ccctggctgt cctggaactc 11821 actctgtaga ccaggctggc ctcgaactca gaaatctgcc tgcctctgcc tcccagtgct 11881 gagattaatg acgtgcacca ccactgcctg gcattttttt aaagttttaa tttgagaccc 11941 tttctcacta acttgctcac actatcctcg aactcattac gtgggctggt ctagaaattt 12001 cagtcaactt gcctcagctt ctcaagtagc tgggattaca ggcaggtgct accacttctg 12061 gccatgatca ttgttgttgt tgctattatt attattatta tttcactgat acttcatatc 12121 acaacacaga atagtaactg tagttcatga ccaacaaaaa agttgtacag tttctctgtt 12181 gtcttatttt gatctggatt ttgatttgtt tcatggcact gggcaggcta atctcaaact 12241 gcatggctca aatagtcctt ctgtcttaat ctcccatgtt ctggaagtat aggtatcact 12301 acacctagct gtattttcat attttaaaaa aaacgcctaa gaattgctat tgaatgaggc 12361 ggagagatgg ctcagcagtc acaagtactt gtagaggact ggggtttggt tactagcacc 12421 tcaaagatgg cttacaatta tctgtagctc caattccaga ggatctgtgt ggtaaagctg 12481 catgtatatg ggcatgcagg taaaactgct aaacatataa aaatagctca gtcttaagag 12541 aattggtcga ctttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 12601 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 12661 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 12721 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga 12781 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 12841 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 12901 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 12961 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 13021 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 13081 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 13141 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 13201 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 13261 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 13321 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 13381 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 13441 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 13501 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 13561 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 13621 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 13681 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 13741 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 13801 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 13861 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 13921 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc aggtcgagta 13981 gggataacag ggtaatggat cctcgttggg cacctgcact catgtacgca ggcccacata 14041 tagatgtaca cacgcatatc attcaaaata gaaattattt atacaagtag tgcgttggag 14101 agctggctca ggagttgtta atgcttgcta cccttgcaaa ggacccaagt tcaatttcca 14161 gcacctgtgt tgggcatctc acaaccacat gtaaatctag tttcagggga tttcacccct 14221 cttctgaact cttcaggcac ttatacccac aaatggacat ctccacatag agacacatac 14281 ttttaaaaaa gtaatctagg gtctagagaa atggttatag accacggact gggatcagtt 14341 cccagaagcc acatcctgtg gctcacaatt gcctgtgaat caagttctag atctggtggc 14401 ctcgtctagc ctgcagtcat caacaaacac atgcacacat ccatacacac ataatttaaa 14461 agaaagcgaa agtaacctat aatgctaaac aaagcacatt ctcagtgtag actgcagtgg 14521 tgatgaacct tgaaaatata ttaatagaag ccagacacgt aagtcacaaa tggtataact 14581 caatttatat gaaattccca gagagtccaa agttaaaaaa aacaaaaagg cagattattg 14641 gttgtcagta gctacagaga tgggacattg aagatattaa taggtatagg gttgaggatt 14701 catggccttt ccctcttcca tgttagcatg tctattgttg ttaaccttat tcaactcatg 14761 ttagggcaat catattgatg agacttaatg agtatagctt gacattccta gaagacagag 14821 tctcacatca gactctctga tcctctgact cttagaatct ttcaacaccc tcttctgaaa 14881 tgatctctga gcctttggtt gtgactgaat tatgagaaag aattttatgt tgaatgaatt 14941 ttacttcagc atttttacaa aactgaagct ggggatggcg tttcatgcgt gcagtcccta 15001 ccatttgcct ataagcctgg aggaggcaga agtagaatta ggcttttcag ggacatcttc 15061 agctgcatca tgagcttgag gacaacttga gctacataag attcccatcc ccacccccac 15121 acacacacac acatctagta tgtacctagt atgcagtctt ttcatatgtt taaaatgtag 15181 aaaggtatat gtgaagtctc ccttttcccc acaacttagt ccctccaaag tattgcatca 15241 gtttccttgt acatgatttt atgcatatat aagatacact atttctctct ctctctctct 15301 ccttccctcc ttccctccaa cctctgggta tgagttgagg gttatgtatg tttcaagttg 15361 taataagaat tgccactcaa acatttcatc ccattttaag ttaagtttgt ctgattttcc 15421 actttagaag ggcagccatg atggctcatg cctataatcc catcctagca cttgtaagcc 15481 taatacataa gactggagga ttgtcatcag cttgaggcta gcctggtcta cctaatgagt 15541 tccagccagc caagagtaga atataagacc ctgtctccaa aaaatgtacc aaaggggctg 15601 gaggggtaac tcagtggtta agaacagtag ctgctcttcc agaggacctg ggttcaattc 15661 ccagcaccca catggcagct tgcaactatc tgtaactgtc cagctttggg atatccgaca 15721 ccattatacc aattacataa aatgaaaaaa ttgtaaaatg tgctaaaatg agtggggagg 15781 gggagcatgg tgtggttctg tgggcttggg attttgccat gaatccatga ctatatctca 15841 gcaatatgtg gattatttct ggaaacctgt ggggcatggg tgtatgggaa gaggggagag 15901 agagagagcc cgcatccagc cagagttcct gtgctctggg caggcggacg tgggaggact 15961 gctggacgat ttccacttgg ccctgggtgg gcatctggct gtgtgaagcc actgacccca 16021 ctcagcgggg ggtagataag gggcatttcc cataccaggt tctcagactc cagcattcca 16081 cgctggatac agcagaagag ctgagaacag aataggggcc ccagggagac actctcgtcc 16141 cagagataaa gaaggcagag ggagagagat tcctacgcag gcgagaggcc ttagtctggg 16201 ccttgatgaa cagagacggt ctatggtttt agagctttat tgtagaaagg cagggggaaa 16261 gagagaaggt agaaagagag agaccagcca tggccaagag gagagaaggg gggaaaggag 16321 agagagaaga aaggctagag agtaagaaag gttaaagaga cagaagtaaa agcttagagt 16381 aagagagaga gagagagcaa gagggagagt aagaaaagta aaagcttaag agagcgagga 16441 ggggccagcc cctctaatag tgggcttgtt atcttgttgt tgctaggtaa ctgtgaagcg 16501 tctagcctga aggccagaag cttgggacat tgtctatgtg actgaaagcc atgagcctct 16561 actgtggggg ctgtggaggt ggtaacttgg acaggagcca ggggttccag gagacatgag 16621 ggaatgcctt ctgtcccatg taggtaggaa ttaccaccac cggctttcac cgtagttcac 16681 tggagactgt ttgtacatag cccattgccc cacagaaacc aggcactcac tgtttcattt 16741 gcataagaat tagaaggctt tattttggct gggattggtg acttgcccca gaaattccag 16801 cttttgggaa gccgaatcct ggagaaccag gagttcaagg tcatcctcag gtacctcatt 16861 tgttcaagac cagcctgtgc taccatgaga ccttgtccct actcccctca accccaaaac 16921 acgctgtttt cattttatag gttgtactaa ccatggagga tggtcatttg cctttctctt 16981 gatttcagaa gcgtagcgcg ggctttgcac catgatctag tgtttccaga taggcttgcc 17041 tctccacagc caggcttatc ctgcttcagt cccatgtttg gcaagggcaa catatgactg 17101 aagaacttgc atagccttat ggtgtatcat gactcttagc cccagtttat aactgtctaa 17161 cagctggtgg cttttgtgaa aaaggaaagc atcatttgaa cttttaactt ttcagctgtt 17221 tgttttgact ttgcataaat actgagacta gctaggaaat gagcttttgt ttcatttgct 17281 aaaaatgatt tttttttcat gaggcaattt caagcaattt gaatttagca atctctactt 17341 gtctgaaaag aagttattaa ttaaaaaaaa acaaacaaac aggaacctat tatgctggct 17401 agtcacatga aagaatccaa atgaacacag tcaaggctga atcaacacat gaaattattt 17461 tctaattccc caaattacat ttatgctaat tagtatgtag taaaacattt taattgtttc 17521 caatgttaaa cattacagaa ataattttga tgttttccta ataacccagc ctttgatcat 17581 gtttgcattt atttgattgt tgtcagttta ttaaataatt aagtaagagc aacaagataa 17641 aatgacttca tgagagtcct tttctgtaac ttagaacgtc agtagtcata ggaactgcgg 17701 tcgttccctt acctttcaga agtaggctaa agagttgaac gcaaaggtgg tttttcgaat 17761 cttcctgatc cttaagcccc cactaggttt atattctata tttgatatcg gattcgaacc 17821 cctcgagctt aggatccgga acccttaata taactaagtt attaggtccc tcgaagaggt 17881 tcactagtac tggccattgc ggccgcttcg aggctgcctc gcgcgtttcg gtgatgacgg 17941 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 18001 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 18061 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag 18121 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 18181 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 18241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 18301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 18361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 18421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 18481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 18541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 18601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 18661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 18721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 18781 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 18841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 18901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 18961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 19021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 19081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 19141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 19201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 19261 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 19321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 19381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 19441 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 19501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 19561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 19621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 19681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 19741 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 19801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 19861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 19921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 19981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 20041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 20101 ccgcgcacat ttccccgaaa agtgccacct gacgg //