LOCUS Ple53\(pEMS1199) 19968 bp DNA circular 17-JUN-2009 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2364 - 5' - GCGTATCACGAGGCCCTTTC- 3' Tm=56.0 Deg C Antisense primer: oEMS2753 - 5' - GCTGCCACCAAGTGGGATAAA-3' Tm=56.3 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 735 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|521216110| COMMENT VNTDBDATE|521291202| COMMENT LSOWNER| COMMENT VNTNAME|Ple53 (pEMS1199)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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COMMENT 5.10859 1 0.082322) 0.8 1.8 0) COMMENT #153=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 2081903016 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "EGFP" "@N" 1 COMMENT 12.7987 3.48466 0 -10 2.67247 1.17389 #152#) COMMENT #154=(CScratch COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #108# 817 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.88239 1.9 COMMENT 0.082322 1) COMMENT #155=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 26741756 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Tetracysteine residue" "@N" 1 16.2902 COMMENT 1.74233 0 -10 10.0405 1.17389 #154#) COMMENT #156=(CScratch COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #108# 718 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.87108 1.9 COMMENT 0.082322 1) COMMENT #157=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 27799900 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "NLS" "@N" 1 COMMENT 12.873 0 0 -10 1.99811 1.17389 #156#) COMMENT #158=(CScratch COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #108# 652 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.84658 1.9 COMMENT 0.082322 1) COMMENT #159=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 2081903016 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "MFRT" "@N" 1 COMMENT 13.1215 -1.74233 0 -10 2.72243 1.17389 #158#) COMMENT #160=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #108# 652 100) COMMENT (LOGPEN 0 8 13395507) 10 1 4.78783 1.9 COMMENT 0.082322 1) COMMENT #161=(CLabel (CWidget 0 (0 0) 1 2 0 0 #100# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "SV40 early polyA signal" "@N" 1 16.8915 COMMENT -3.48466 0 -10 11.0645 1.17389 #160#) COMMENT #162=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #108# 718 100) COMMENT (LOGPEN 0 8 13395507) 10 1 6.03222 1.9 COMMENT 0.082322 1) COMMENT #163=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 26570932 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "oEMS2753" "@N" COMMENT 1 8.62528 10.454 0 -10 4.79547 1.17389 #162#) COMMENT #164=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #104# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 5.12398 COMMENT 6.22983 1 0.082322) 0.8 1.8 0) COMMENT #165=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 2081903016 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Ple53 (DCX-B)" COMMENT "@N" 1 11.6463 8.71165 0 -10 6.46889 1.17389 COMMENT #164#)) (CObjectList)) COMMENT #166=(CGroupWidget COMMENT (CWidget 22 (7 22 0) 1 2 0 0 Nil 1634541683 100) COMMENT (CObjectList COMMENT #167=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #1# 0) 1 2 0 0 #116# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 COMMENT 0.00188497 0.385791 1 0.082322) 0.8 1.8 1) COMMENT #168=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 27801844 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -4.38824 12.1963 0 -10 2.87229 1.17389 #167#) COMMENT #169=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #2# 0) 1 2 0 0 #116# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 COMMENT 1.93618 2.38417 1 0.082322) 0.8 1.8 0) COMMENT #170=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 30547692 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 COMMENT -11.6067 -6.96932 0 -10 2.87229 1.17389 #169#)) COMMENT (CObjectList)) COMMENT #171=(CGroupWidget COMMENT (CWidget 27 (7 27 0) 1 2 0 0 Nil -461 100) COMMENT (CObjectList COMMENT #172=(CScratch COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #108# 798 100) COMMENT (LOGPEN 0 8 13395507) 10 1 6.25653 1.9 COMMENT 0.082322 1) COMMENT #173=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 1944545584 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "oEMS2364" "@N" COMMENT 1 3.23685 12.1963 0 -10 4.79547 1.17389 #172#)) COMMENT (CObjectList)) COMMENT #174=(CGroupWidget COMMENT (CWidget 86 (7 86 0) 1 2 0 0 Nil 1634541683 100) COMMENT (CObjectList COMMENT #175=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #0# 0) 1 2 0 0 #104# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 0.730741 COMMENT 1.92833 1 0.082322) 0.8 1.8 0) COMMENT #176=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 27792060 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "5' homology sequence" "@N" 1 -16.2999 COMMENT 3.48466 0 -10 10.4901 1.17389 #175#) COMMENT #177=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #3# 0) 1 2 0 0 #104# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.3826 3.52804 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #178=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 1128485972 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "3' homology sequence" "@N" 1 -7.56613 COMMENT -12.1963 0 -10 10.4901 1.17389 #177#) COMMENT #179=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #104# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 3.52804 COMMENT 4.77023 1 0.082322) 0.8 1.8 0) COMMENT #180=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #100# 4063800 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "HPRT complementary sequence" "@N" 1 16.9781 COMMENT -6.96932 0 -10 14.9609 1.17389 #179#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #181=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -322 100) COMMENT (CObjectList) (CObjectList)) COMMENT #182=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -391 100) COMMENT (CObjectList) (CObjectList)) COMMENT #183=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1019 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #184=(AnlzFontBackColorItem 53 72 43633766) COMMENT #185=(AnlzFontBackColorItem 138 766 40304639) COMMENT #186=(AnlzFontBackColorItem 788 3657 40304639) COMMENT #187=(AnlzFontBackColorItem 767 787 43633766))) (CObList) 3285093 COMMENT (CStringList) 1277193313 1818583649 (CObList))) FEATURES Location/Qualifiers misc_recomb 13831..17642 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(18741..19962) /vntifkey="22" /label=AmpR misc_marker 12380..13805 /vntifkey="22" /label=AmpR misc_recomb 8739..12384 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4785..8738 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 13812..13829 /vntifkey="21" /label=I-Sce\I misc_feature 3667..3700 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 3701..3711 /vntifkey="21" /label=Kozak\consensus misc_feature 3708..4427 /vntifkey="21" /label=EGFP misc_feature 4428..4463 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4464..4536 /vntifkey="21" /label=NLS misc_feature 4542..4575 /vntifkey="21" /label=MFRT misc_feature 4729..4779 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..3658 /vntifkey="21" /label=Ple53\(DCX-B) /note="pEMS1306 - DCX-B MiniP is made up of the region "LongProm"" primer 54..73 /vntifkey="27" /label=oEMS2364 misc_feature 768..788 /vntifkey="21" /label=oEMS2753 /note="Note: there is a A>G mismatch at bp 7 (to 5' end) but still amplifies" BASE COUNT 5371 a 4408 c 4389 g 5800 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccgg aacacctatt aatgcccaca tatctcaaac aaggaaatat 181 tgatcctgcc tatccctctg ctctctatta tatataaagc aaacaaagtc atttatccta 241 ggctgtctca taaaaacatt ttgtagcttc cacagtagca ttgcaaagcc tatattatga 301 ggaattttcc tctgaaatac attccaaatg gttcgtgttt aagttttatt atcccatcca 361 ttgttgtatc ttcaccagaa ataaaatgat ttttagttct gatgcaaaat taattgctac 421 atatttactg aatgcttatg atgtgcaaga aagaagtttt aagacattag ctttgttgga 481 atttccatat tttcctgccc ttattccttg atttttcttt gcctctagcc tgaataatgt 541 tgatttttca tgaggttgcc acaaggtaag gcaacaacac atggaagaaa atagggcaga 601 cacatgcatt cccctgaggt acttgtatag taaccaagaa atcagcagca aggtgttggg 661 gggacttaac taggtaggtt gcacatttga ggctatccac tcaactaaca gacagttcca 721 ggctttgctg agtcaacacc tttcacagaa gaaagaccat catatatttt atcccacttg 781 gcggcagctt acaataaaac acatgcagag aaaatgctta aatataaaag ttcaacagca 841 ctatataaaa ggaagggtta attatactag gaatcagata taaaataatt actgagcatg 901 ctcttagctc tgagcttcct ggaagccaag caaaaataga accatgatag ttcaaggctg 961 ctacatattg atgcatgtag cttcaattgt gaagatggta gcatccccct accccattta 1021 acctctcacc tttctctttt gttttatagt tcggcctgat ctaattagtt caatttggat 1081 gcttccttga gttttttttg taacatattt tatataaaga agtcagttag tgacaaataa 1141 gcagtttgag gagaaatctg ttaatattta ttttgtagcc atcagattta cttcacatag 1201 aaaggtcttt gggttgggtt tgaacttcca aactctcaaa ggtaaatgcc acattaacct 1261 ttcattaacc aaattcttac accaagctga tagatttggg atgtcctttt tacttctatc 1321 ttccataata ttctaaaatt attttccctt gttttgttcc tatcctactt cctcttagtc 1381 tactttgttg acttcattaa aaaacaaaaa accagttgtt ggatacttga gctaaactgc 1441 cttaaagaat ctgcagattt tattttattt tttttctctc aagagggtaa aaggaagaga 1501 gctacaattt ctaagaagcc tggcttggct gtctgagtct ggcccccagg cagattaggc 1561 caaggttttg gccaagtgaa attgccaatt ttctaaaaga aagggctagc acattgctca 1621 ttagagcatt ctgattttgt ctgcgcaatc tttctgctac cccgcaattt cctgttggtt 1681 ataaatgaaa cctttctagc tgttaatgca gcctgtgaat ttttttaaaa gcatgtaatt 1741 aatcatagga ggttgggggg attcactaag cctgagttac atgggagaag ctggacaagg 1801 cactaggacc tagaaggcat ctatccaccc tggcaggaat ttcttgcttg gagctcagac 1861 aacaaaggca tagagagatt ggttttcttt ctctcagcat ctccacccaa ccagcagaaa 1921 accggtgagt ggggctttta agtgattttc aagaagaatg taacagatgt caaacgggaa 1981 aagcacaagg caaagcctgc tctctctgtc tctctgtctc ctcttctcct tttttgcctt 2041 attctatccg attttttccc taagcttcta cctgggattt tcctttggaa aagtgagttt 2101 gatgttcctt tgttttcact gtgatgttaa tttagaataa tactacctct gatcctaaag 2161 caaagcaaag ccttactggc atgcctgggg aaatgtttgc tgcttgcctt gaggaggtgg 2221 ggtctcttac cactgcaggt tgtctgacag agacaatgct gagctcagca taggtcatgg 2281 tgacattgga aaaaaggcgg aattgagcct ggcagaccca ttaggcacca gtctttctta 2341 tctcctgtcc tcctggtccc ttgcaaatat attgatgtgg cagtgtgtag cagctgagcc 2401 ctgcttgctt tgtgagtcct tttatcccca tctgtgagat gcatgttaat agtttggctc 2461 gtaggatgtc actacatttg ctagcatttg tggcttcagt tgtattgggt ttcatgtttt 2521 gattgtttgg ggttcttggt gggggagggg gttcaacaga agggagaaaa gcaaagcctg 2581 acaaatgacc atcttttctc agctaatgca cctgggcaat atacaagttt ggggtgaatt 2641 gcctgctgtg agggtaaatg tcacttcaat taaggtagaa acccagaaca atgaaaggtg 2701 tgcttccttc taaaggtccc gtatgctgtt cggagagtca tttgtgaatc tttcaacaat 2761 taaattattc cattaagagg tgttgctgca tcagtgggga gggggtggag cacctggggg 2821 ggaaaaaaaa ggattttgtg aacaaatgga accgggggaa gacagagcta gtaacttgtt 2881 aaataactta tttttctaat cctttttccc cccagcttat ttcttatgaa tgtcggatag 2941 ctgcaccagc ttggtgggga aagggtttga tgaatagcac aaagacactg gctgttccct 3001 ggaggctgtc cctttaaagg agaatcttag tttattctgg ggggagggga tgcacacatt 3061 agagtaggaa agagggcttg gaataaaatg aaaacactcc cccttcatag tcattgtact 3121 gaaatgcaaa gactgcttcc taagctggag atgctaacct tgggtagctc cttctgttct 3181 cttcaagggg aattttgtca ggctatggat tcatttacaa ctgttagtca tgtgggcatg 3241 tgtgaggaaa cagatgccag ttttaatgta tttagcccga agttccaatt tgataggagc 3301 cactgtcagt aagtctcagg attttcagct atttcaaaat ctccccttct cctctgtctg 3361 gaacagtgcc aagagtgcct ccctctctat ctcttactcc caacccccac aaccaccagc 3421 acccccgccc agcccctcct tcttctctat taagatcaat attcctgcag gtcaggggca 3481 agcagcagat gggtcacagg cttttttcaa ccagttcttt tcacaagcag cagattgcag 3541 atctggatct ggctaatatt taaaatccct tcttttttcc ttctccttgt ccctttttgt 3601 ttttgcctct cttcaccccc atccctttct cccacgctca ggtctctgag gttccaccgg 3661 cgcgccgaag ttcctattct tcaaaaggta taggaacttc cgccaccatg gtgagcaagg 3721 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 3781 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 3841 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 3901 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 3961 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 4021 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 4081 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 4141 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 4201 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 4261 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 4321 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 4381 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagttattt ctgaactgct 4441 gcccgggctg ctgcatggaa ccggatccaa aaaagaagag aaaggtagat ccaaaaaaga 4501 agagaaaggt agatccaaaa aagaagagaa aggtaatata agaagttcct attctctaga 4561 aagtatagga acttcagcgg ccgcgactct agatcataat cagccatacc acatttgtag 4621 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 4681 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 4741 gcatcacaaa tttcacaaat aaagcatttt tttcactgcg aattctcgta aaactcttca 4801 tggcagtagt tattattctc tctctctctc tttttctttt ttcttgagac aggatatttc 4861 tctgttgccc aggctggagt gcagtggcac agtcttggct cactgcagcc tggacctcct 4921 gggctcaagc catcctccca cctcagcctc ccaagtagct ggggctacag gcacatggcc 4981 accaggccca gataattttt catttttgta gagactgagt ctcaccatgt tacccaggtt 5041 tattattctc attttttaga tgaagagact gaggtccaga gaagctcaat gacttgccta 5101 gttttacaaa tctcctgcca tcacataccc ctcagcgtcc ttaataagag ggaggccacc 5161 aactatgtgc tgggcactgt ggtggatgct ggagctatag ggttgagtat ataagaaatg 5221 gtgttgctgg agcaactgtt gcttgcttac ctgacctatc tgagaattaa ttagcagggg 5281 aacatatttt tgttttcaga ttcaatataa gaacttgtgt gggcaaaaat aaagatcagt 5341 agtaataaca gtagttccca tttgctgact gtactgtcct aagtgcatat atatatacat 5401 acacacacgc atacctatac tcctctaata ctcaaaatga tcctgtttat gtattgttaa 5461 tatgctcatt ttatttttaa atttttattt atttttattt ttatttattt ttgagacgga 5521 gtctcattct gtcgcggagg ctgaagtgca gtggtgcgat ctcagctcag tgcgacctcc 5581 gcctcccggg ttcaagtgat tctcctgcct cagcctccgg actagctggg attacaggcg 5641 cccgcctcca cgcccagcta atttttgtat ttttagtaga gatggggttt cgccatgttg 5701 gccaggctgg tctcgtactc ctgaccttga gtgatccacc tgcctcggcc tcccaaagtg 5761 ctgggattac aggcatgagc caccgcgccg ggctaatatg ctcattttag tgaggcaaaa 5821 atagaggctc agagtctgat ttgtacaaaa ctacagagca gttaagtgtc ctctcagatg 5881 tgtaccctga tctgggtgac tctaggactc taggtctcaa ctgttacaac cagttaaggg 5941 tttggggaag cactgggcca agagtcagga aaatggaagc cacaggtagt gcaaggtctt 6001 gggaatggga cgtctggtcc aaggattcac gcgatgactg gaacccgaag agccggggcc 6061 cggtttacgg ccgccatgaa gcaacgcgcg ccggtaggtt tgggaatcag ggagccctct 6121 gaataggaga ctgagttggg agggaaaggg gcttcgctgg gggagcctcg gcttcttctg 6181 ggagaaaatt cccacggcta cctagtgagc ctgcaaactg gtaggcgccg gcgtaggcgc 6241 gcgggcgggg ccgggggcgg ggcctgcggg gcgtggcggg gcgggcagag ggcggggcct 6301 gcttctcctc agcttcaggc ggctgcgacg agccctcagg cgaacctctc ggctttcccg 6361 cgcggcgccg cctcttgctg cgcctccgcc tcctcctctg ctccgccacc ggcttcctcc 6421 tcctgagcag tcagcccgcg cgccggccgg ctccgttatg gcgacccgca gccctggcgt 6481 cgtggtgagc agctcggcct gccggccctg gccggttcag gcccacgcgg caggtggcgg 6541 ccgggccctg aggcgcggga tcgatccatg acaccccccc gccccccata tctcactata 6601 attcactgtc aaatggcttt tatgatttgg agtttgggga tacttttaaa gacagggtct 6661 tggtagctcc tagatggctt gggattcact tagtaaccct tgctggccta aaattcagga 6721 acttctacct catcttccca agtactgggt tagtagggat gtccagcata tttagcttgc 6781 tttaatgatt ttgctgtact tttttctgtg tgtacaggtg catgtatatt tataaaacta 6841 acataatata ttttaccccc aaagtaccat aaccctaatt aatgctatta aatgttttgt 6901 atgctgggct ctgggcatac taatataacc aatattatac aatgcaaaac aattttggaa 6961 tatcttctgt aaaattatct tattagtaag ttgtaaccat ctcagacttc taagtttgag 7021 tttgtaaagg atcagttttt tttggtgacc tacacacaca cacacacaca cacacacaca 7081 cacacacaca ggggggtgga ttctgagaat ttagagaatt tagatggtta ataccagttt 7141 ttatttttat tctgtgtgat atttatttat taagatttat ttatttatta catgtaggta 7201 cactgtatct gtcttcagac acaccagaag agggcatcag atctcgttac agatggttgt 7261 gagtcaccat gtggttgctg ggatttgaac tcaggacctc tggaagaaca gtcagtgctc 7321 ttaaccgttg agccatctca ccagccccgt gatattttat ttaacttact agaatatact 7381 gtgttaaaac atattttagg ggattgggga gatggcccat tctgtaaagt gtctgccatg 7441 tcatcttcag gacctgcgtt tgtaacccca gcatggagaa gacagagaca gggagacccc 7501 tgggcttcct ggcaagctag actagcaaat ttggttaact aggttcaatg agagactcag 7561 tctcaaaaca taagaagtga attttttaga cccaatttca atctctgaac tccatacgta 7621 tgcacatgta cctgcatata aatatatata cacacccata aacacatgca cacacaaaaa 7681 atggttcaaa agttcttaaa atgtataaga gtagaaagtg tagagccggg agtggtggcg 7741 cacgccttta atcccagcac ttgggagcag aggcaggtgg atttctgagt tcgaggccag 7801 cctggtctac agagtgagtt ccaggacagc cagagctata cagagaaatc ctgcctcaaa 7861 aaaccaaaaa taaagtgtat atactaatct ttcatgccta aaatatttat ttttgccaca 7921 tttttcatac ctctttaata tttttctttt tctctttgtg cttcaaagga aattttgtgt 7981 catgttttac ttttcattct tgactctatt cagcagtaag acgcagcatg gctttcttag 8041 agaagtataa tctattacaa aacggtaaca tttaactgct ctacataccc tcttgtagct 8101 aaattcctct gttaaactaa gacttattat ctaaacgtaa ttaaatgttg atatcttata 8161 atgctccact ttgaaacagc tgtctttctt ttgcagatta gcgatgatga accaggttat 8221 gacctagatt tgttttgtat acctaatcat tatgccgagg atttggaaaa agtgtttatt 8281 cctcatggac tgattatgga caggttagta agacctcgat tgaagttatt tttagagggt 8341 gtgtgtgtgg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta agagagagag 8401 gagaggcatg tgtgatatat atgtgaagtg tattgcatgt acatggaatc cagagaagga 8461 cattaggtgt cctactctgt tgctctctta ttcccttgaa tcagtgtctc ttgctgaacc 8521 tggggcatac atactgattt ggctaggctg cctggctagc aagacccagg gatctgcttg 8581 tctctgcccc ccagtgctga cgttacaagc aggtgcaacc acatctggtt tttatatggg 8641 tactggggga tctgaactta gggcctgatg cgtgcccaag tgctctcacc cgccaagcca 8701 cctctccagc caaagggttt tttttcttgt attaattcta acttagcaac tgttgagtag 8761 ttgatgtttg aatataagct aagccaactt attagaataa atgttttcta ttttttactt 8821 gtatgtgtga gtgcctgcat gtatgtatgt gaagcatatg tattccagga tgtcagaatt 8881 gtgatggatt ccctggaact ggagttacag accattgtga accactgtgt atgggtgctg 8941 ggaactgaac tttaggtcct ctgcaaaaac agtaaggatc ttaactgata aactatcttt 9001 ctagccctaa ggctttctac ttttaaagtt cgtttctctt tctctctctc tctctctctc 9061 tctctctctc tctccctctc cctccctcct cctcctcctc ctctttcccc cctctcgcct 9121 cctctttctc tctcccctcc ctctctctct ctcacacaca cacacagaca gagagaggag 9181 agagaaagga agccttacta attaattacc tagttttaga cataactagt gggaatccat 9241 agtaagtaaa tcaacattct tgaagagagg ttagaagaat tggctctcgt tgtccatcga 9301 ggctgtataa gattattctt ctgttttctc agcttaccat acatattttc tacgtagaca 9361 ataccaattc aaatacactg tacgtaaagt atatgcgcac acacacacac acacacacac 9421 acacacacac acaagttaca tattgtcata tagtactaaa atttatggac tttttcatat 9481 taatacatct agagctatca tgttttttat cttttaaaaa ttactttacg gggctacaga 9541 gatgtctcag cagttaggag cactgactgc tcttccagag gtcctgagtt caattcccag 9601 caaccacatg gtggctcaca accatctgta atgggatctg atccactctt atggtgtgtc 9661 tgaagacagt gacagtgtac tcatatacat gaaataaatc tttaaaaaat tactttacca 9721 tcagggtata tatatatata tatatgtgtg tgtgtgtgtg tgtgtgtatg aagtatttgc 9781 tatttgagag ggtctcactg tttagctcag tctggcttgg aacgttctgt atagcccaga 9841 ctggcctcaa ctcagcagtc cccctgaaag gctctcatat gtggagatta caggtatgtg 9901 ctactgtgcc cagccctaca tgttactttg tttttgaaat agggtatcaa atagcttaga 9961 ctggcttcta agtcagtgtg tagctaagga tggtctgagc ttctgaacct gctacttcaa 10021 ctcctggtgt gccatcatgg ccagtttgca cagttgtgag agtagaactc aggacctcct 10081 atgtgttact agacatgttc tctaccagct gagctacatc ccaattcgcc tacatttact 10141 tttatttttt aaaagattta tttatgtata cagcactctg cctgcatgta catctgcatg 10201 ccagaagagg gcaccagatc tcatcataca tagttgtgag ccaccatgtg gttgctgggg 10261 attgaactca gggcctctgg aagagcagtc agtgctctta acctctgagc catctctgta 10321 gccccctaca tttattttaa agattatagg ctattctatt catagatgtg ccataactga 10381 tttaaccaga tcttcattat tttgtactta gtagagtata ttagagggaa tgaatcttaa 10441 atacacattt tgacacttgc tcagctttag acaagtaaaa atcactggtc aaggaaatgg 10501 tgcttagtgg atgcttctga actgtcaaat tgtcctccaa caagttacat cattttatac 10561 ttccaacgtt atgtgagagt tcatttttct ttaggctctt ctagtatttt atatttgaga 10621 aataaaattt aaccaaaatt tgccaatcta gtaagcttgt ttttatttgt atctttaaat 10681 tttatgatag atgttatagt gtactctcct ctcccctccc ctcccctctc ctcctcagca 10741 ggatcttgtt atgtagccct aagggatcta atacttactc tgtagtccag gctgtccttc 10801 agctcatgag acacaccatc atgcttggca tgtttcttgt tctgaaaggt atctggtttt 10861 aattttggtt gagctggtgt gttggtgtat atctgtaatc tcagcacagg caagtagaga 10921 caaggattat gattacagtc cagcctgggc tatttagagg gattctcttt aatctttcct 10981 catgccccaa aatcttacct ttggtatatg aaaaatagtc tccacttctg caaaatattg 11041 ctttatgaag taagaattaa ttcccttcat agagacaagg aatgtgtcct gtaaaagttt 11101 aatgtgtaag aagtatttgt tataaaagat aaatattcag aatcttcttt ttaattcctg 11161 attttatttc tataggactg aaagacttgc tcgagatgtc atgaaggaga tgggaggcca 11221 tcacattgtg gccctctgtg tgctcaaggg gggctataag ttctttgctg acctgctgga 11281 ttacattaaa gcactgaata gaaatagtga tagatccatt cctatgactg tagattttat 11341 cagactgaag agctactgtg taagtataat taacttataa ttaaaaaaat agggccattc 11401 tagttttatc tatatttttt ttaaacttgt gcaaactatg ctacataatc aacttacttg 11461 attatgtaat atatatagta atagttggaa ttactattat tacatattta tttttctgtg 11521 ctggggtaga acccagggaa ctaagtactg taataaccga gcttcattca tcctagtcat 11581 ttttaatttt tttttttttt tttgtttttt cgagacaagg tttctctgtg tagccctggc 11641 tgtcctggaa ctcactctgt agaccaggct ggcctcgaac tcagaaatct gcctgcctct 11701 gcctcccagt gctgagatta atgacgtgca ccaccactgc ctggcatttt tttaaagttt 11761 taatttgaga ccctttctca ctaacttgct cacactatcc tcgaactcat tacgtgggct 11821 ggtctagaaa tttcagtcaa cttgcctcag cttctcaagt agctgggatt acaggcaggt 11881 gctaccactt ctggccatga tcattgttgt tgttgctatt attattatta ttatttcact 11941 gatacttcat atcacaacac agaatagtaa ctgtagttca tgaccaacaa aaaagttgta 12001 cagtttctct gttgtcttat tttgatctgg attttgattt gtttcatggc actgggcagg 12061 ctaatctcaa actgcatggc tcaaatagtc cttctgtctt aatctcccat gttctggaag 12121 tataggtatc actacaccta gctgtatttt catattttaa aaaaaacgcc taagaattgc 12181 tattgaatga ggcggagaga tggctcagca gtcacaagta cttgtagagg actggggttt 12241 ggttactagc acctcaaaga tggcttacaa ttatctgtag ctccaattcc agaggatctg 12301 tgtggtaaag ctgcatgtat atgggcatgc aggtaaaact gctaaacata taaaaatagc 12361 tcagtcttaa gagaattggt cgactttttc gccctttgac gttggagtcc acgttcttta 12421 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 12481 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 12541 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg 12601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 12661 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 12721 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 12781 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 12841 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 12901 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 12961 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 13021 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 13081 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 13141 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 13201 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 13261 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 13321 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 13381 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 13441 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 13501 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 13561 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 13621 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 13681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 13741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 13801 accaggtcga gtagggataa cagggtaatg gatcctcgtt gggcacctgc actcatgtac 13861 gcaggcccac atatagatgt acacacgcat atcattcaaa atagaaatta tttatacaag 13921 tagtgcgttg gagagctggc tcaggagttg ttaatgcttg ctacccttgc aaaggaccca 13981 agttcaattt ccagcacctg tgttgggcat ctcacaacca catgtaaatc tagtttcagg 14041 ggatttcacc cctcttctga actcttcagg cacttatacc cacaaatgga catctccaca 14101 tagagacaca tacttttaaa aaagtaatct agggtctaga gaaatggtta tagaccacgg 14161 actgggatca gttcccagaa gccacatcct gtggctcaca attgcctgtg aatcaagttc 14221 tagatctggt ggcctcgtct agcctgcagt catcaacaaa cacatgcaca catccataca 14281 cacataattt aaaagaaagc gaaagtaacc tataatgcta aacaaagcac attctcagtg 14341 tagactgcag tggtgatgaa ccttgaaaat atattaatag aagccagaca cgtaagtcac 14401 aaatggtata actcaattta tatgaaattc ccagagagtc caaagttaaa aaaaacaaaa 14461 aggcagatta ttggttgtca gtagctacag agatgggaca ttgaagatat taataggtat 14521 agggttgagg attcatggcc tttccctctt ccatgttagc atgtctattg ttgttaacct 14581 tattcaactc atgttagggc aatcatattg atgagactta atgagtatag cttgacattc 14641 ctagaagaca gagtctcaca tcagactctc tgatcctctg actcttagaa tctttcaaca 14701 ccctcttctg aaatgatctc tgagcctttg gttgtgactg aattatgaga aagaatttta 14761 tgttgaatga attttacttc agcattttta caaaactgaa gctggggatg gcgtttcatg 14821 cgtgcagtcc ctaccatttg cctataagcc tggaggaggc agaagtagaa ttaggctttt 14881 cagggacatc ttcagctgca tcatgagctt gaggacaact tgagctacat aagattccca 14941 tccccacccc cacacacaca cacacatcta gtatgtacct agtatgcagt cttttcatat 15001 gtttaaaatg tagaaaggta tatgtgaagt ctcccttttc cccacaactt agtccctcca 15061 aagtattgca tcagtttcct tgtacatgat tttatgcata tataagatac actatttctc 15121 tctctctctc tctccttccc tccttccctc caacctctgg gtatgagttg agggttatgt 15181 atgtttcaag ttgtaataag aattgccact caaacatttc atcccatttt aagttaagtt 15241 tgtctgattt tccactttag aagggcagcc atgatggctc atgcctataa tcccatccta 15301 gcacttgtaa gcctaataca taagactgga ggattgtcat cagcttgagg ctagcctggt 15361 ctacctaatg agttccagcc agccaagagt agaatataag accctgtctc caaaaaatgt 15421 accaaagggg ctggaggggt aactcagtgg ttaagaacag tagctgctct tccagaggac 15481 ctgggttcaa ttcccagcac ccacatggca gcttgcaact atctgtaact gtccagcttt 15541 gggatatccg acaccattat accaattaca taaaatgaaa aaattgtaaa atgtgctaaa 15601 atgagtgggg agggggagca tggtgtggtt ctgtgggctt gggattttgc catgaatcca 15661 tgactatatc tcagcaatat gtggattatt tctggaaacc tgtggggcat gggtgtatgg 15721 gaagagggga gagagagaga gcccgcatcc agccagagtt cctgtgctct gggcaggcgg 15781 acgtgggagg actgctggac gatttccact tggccctggg tgggcatctg gctgtgtgaa 15841 gccactgacc ccactcagcg gggggtagat aaggggcatt tcccatacca ggttctcaga 15901 ctccagcatt ccacgctgga tacagcagaa gagctgagaa cagaataggg gccccaggga 15961 gacactctcg tcccagagat aaagaaggca gagggagaga gattcctacg caggcgagag 16021 gccttagtct gggccttgat gaacagagac ggtctatggt tttagagctt tattgtagaa 16081 aggcaggggg aaagagagaa ggtagaaaga gagagaccag ccatggccaa gaggagagaa 16141 ggggggaaag gagagagaga agaaaggcta gagagtaaga aaggttaaag agacagaagt 16201 aaaagcttag agtaagagag agagagagag caagagggag agtaagaaaa gtaaaagctt 16261 aagagagcga ggaggggcca gcccctctaa tagtgggctt gttatcttgt tgttgctagg 16321 taactgtgaa gcgtctagcc tgaaggccag aagcttggga cattgtctat gtgactgaaa 16381 gccatgagcc tctactgtgg gggctgtgga ggtggtaact tggacaggag ccaggggttc 16441 caggagacat gagggaatgc cttctgtccc atgtaggtag gaattaccac caccggcttt 16501 caccgtagtt cactggagac tgtttgtaca tagcccattg ccccacagaa accaggcact 16561 cactgtttca tttgcataag aattagaagg ctttattttg gctgggattg gtgacttgcc 16621 ccagaaattc cagcttttgg gaagccgaat cctggagaac caggagttca aggtcatcct 16681 caggtacctc atttgttcaa gaccagcctg tgctaccatg agaccttgtc cctactcccc 16741 tcaaccccaa aacacgctgt tttcatttta taggttgtac taaccatgga ggatggtcat 16801 ttgcctttct cttgatttca gaagcgtagc gcgggctttg caccatgatc tagtgtttcc 16861 agataggctt gcctctccac agccaggctt atcctgcttc agtcccatgt ttggcaaggg 16921 caacatatga ctgaagaact tgcatagcct tatggtgtat catgactctt agccccagtt 16981 tataactgtc taacagctgg tggcttttgt gaaaaaggaa agcatcattt gaacttttaa 17041 cttttcagct gtttgttttg actttgcata aatactgaga ctagctagga aatgagcttt 17101 tgtttcattt gctaaaaatg attttttttt catgaggcaa tttcaagcaa tttgaattta 17161 gcaatctcta cttgtctgaa aagaagttat taattaaaaa aaaacaaaca aacaggaacc 17221 tattatgctg gctagtcaca tgaaagaatc caaatgaaca cagtcaaggc tgaatcaaca 17281 catgaaatta ttttctaatt ccccaaatta catttatgct aattagtatg tagtaaaaca 17341 ttttaattgt ttccaatgtt aaacattaca gaaataattt tgatgttttc ctaataaccc 17401 agcctttgat catgtttgca tttatttgat tgttgtcagt ttattaaata attaagtaag 17461 agcaacaaga taaaatgact tcatgagagt ccttttctgt aacttagaac gtcagtagtc 17521 ataggaactg cggtcgttcc cttacctttc agaagtaggc taaagagttg aacgcaaagg 17581 tggtttttcg aatcttcctg atccttaagc ccccactagg tttatattct atatttgata 17641 tcggattcga acccctcgag cttaggatcc ggaaccctta atataactaa gttattaggt 17701 ccctcgaaga ggttcactag tactggccat tgcggccgct tcgaggctgc ctcgcgcgtt 17761 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 17821 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 17881 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 17941 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 18001 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 18061 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 18121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 18181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 18241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 18301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 18361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 18421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 18481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 18541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 18601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 18661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 18721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 18781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 18841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 18901 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 18961 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 19021 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 19081 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 19141 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 19201 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 19261 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg 19321 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 19381 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 19441 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 19501 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 19561 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 19621 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 19681 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 19741 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 19801 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 19861 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 19921 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgg //