LOCUS Ple161\(pEMS1357 20438 bp DNA circular 28-JAN-2009 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2454 5'- CAGCCATAGGAGGGATCTTAATGAC -3' Tm= 56.3 Deg C Antisense primer: oEMS2446 5'- TAAAAGGGATCGGCTGGCAG -3' Tm=56.6 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 726 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|508438592| COMMENT VNTDBDATE|508438592| COMMENT LSOWNER| COMMENT VNTNAME|Ple161 (pEMS1357)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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Nil -185 100) COMMENT (CObjectList) (CObjectList)) COMMENT #195=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -464 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #196=(AnlzFontBackColorItem 3460 3629 40304639) COMMENT #197=(AnlzFontBackColorItem 138 2923 50318387) COMMENT #198=(AnlzFontBackColorItem 2949 3459 50318387) COMMENT #199=(AnlzFontBackColorItem 2924 2948 43633766) COMMENT #200=(AnlzFontBackColorItem 3650 4127 40304639) COMMENT #201=(AnlzFontBackColorItem 3630 3649 43633766))) (CObList) 17498167 COMMENT (CStringList) 0 851972 (CObList))) FEATURES Location/Qualifiers misc_recomb 14301..18112 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(19211..20432) /vntifkey="22" /label=AmpR misc_marker 12850..14275 /vntifkey="22" /label=AmpR misc_recomb 9209..12854 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 5255..9208 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 14282..14299 /vntifkey="21" /label=I-Sce\I misc_feature 4137..4170 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 4171..4181 /vntifkey="21" /label=Kozak\consensus misc_feature 4178..4897 /vntifkey="21" /label=EGFP misc_feature 4898..4933 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4934..5006 /vntifkey="21" /label=NLS misc_feature 5012..5045 /vntifkey="21" /label=MFRT misc_feature 5199..5249 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..4128 /vntifkey="21" /label=Ple161\(PITX3-C) /note="1306" misc_feature 3461..4128 /vntifkey="21" /label=Prom\region misc_feature 139..3460 /vntifkey="21" /label=Region\1 primer 2925..2949 /vntifkey="27" /label=oEMS2454 primer 3631..3650 /vntifkey="27" /label=oEMS2446 BASE COUNT 5374 a 4683 c 4871 g 5510 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccga gtgtttgacc attctaagga caggggctca tggggccgag 181 gagctattgc tggaccttgg aatccctaaa acgagacctg gggcagctga actccggtgc 241 cttgggaaca ggtcgagaat gggagggcgg actgcagacc tggggccagg acttgggaaa 301 agggaaactc agggtaagaa ccaggggata gggaaggggg agactcagag gatccggaac 361 gaagccggag acttgaaacc aggatagggg cttcgcgagg cagggagtag ggagtctgga 421 aaccattacc agaaagcgcc ctggaatccc agggcacggg gcaggcggcc agggagcgaa 481 aggggttaca gaggccctgg ccagcggggg agctggcggg gcggccccca gtgtgggggc 541 agttgacggc gcccgatgcc cctcggccta ggctcagact cctggggctg ggagccgggg 601 tgcgcctggt cgcacacgct tctcttccca gctgctccgc agttggaata ggagctgtgt 661 tgtgagagcc cggccgcgac tgagtcagat ggccgctgtg cggatcagag tgaccaggcg 721 gcgggtatag tggatctggc ttggggtgtc tgggcccaga agggtcctga cttgaggccg 781 cctcagaatc ggacagaatg tccgcggtgc atggggctcg gagcggacag aatgtccggg 841 tagcgaagtg ggtctctccg gagcgggagt tgtgtctggc agtggcggac agaatgtccc 901 tggctgggtg gcatgtccat ctacccagca gcgcctgccc agctgctgcg tgtgcgatgg 961 ggtcggctgc ggtgggtcac ctcaaatccc agctcacact ttgcaaactt gtttcagctc 1021 cttcgggctg cgaagctgtg agcagggagc gagagtaaag gctacaacct ggccttgggg 1081 tgaggtgggc tgggacacat gcccccttca attcagaata gatcagagca tcactctgta 1141 cttccagaat ccaaatttga ggacagaagg ccaatgagga gcctgctagt gggaatcaag 1201 accaggaaag gaaggaatag gtgacaaaag gcaatgggaa aacaggaagg agctgggtac 1261 aagcccctgg cagcctcagt tgccccctaa ttccaataca attaaccaga caccccccag 1321 actccttgct ggagtatcag gcgaccaatg gctccttccc tacagtgggc aggagcccag 1381 gccagaagag aaaactacag ccccattagc aaagcaaatc ctgtggtcct gtttcccttt 1441 ttcttcctca gccctaccag tcccaacacc accctggtgg ctgagcggag gtgggaagga 1501 ggctcatgga atccagactc tggagtccaa cagggggcta tctggccctc tcagtctggt 1561 tgggaaggat atcttggccc tacctaacag ccgggtttac tttccctgca gctgggatac 1621 agccctgact ctgctgggcc catacagtga ccatccaccc tgaccccaat tctaaccaca 1681 gctcaattat gctgaagagg acagaacagg gagaacgagt ccatataagt gagcaagata 1741 gcccagctgg ggactgagtt ttcacaagga caagtgcaag gcattgtatt tagcaatgag 1801 taatctaaac agtggtttca acctttctta cccttcacct gtgagtgaca tacaatacgt 1861 attccccata ctcccatgtc cattcctcag ttctaaagtt tgcttattac tggctaggat 1921 tctcaacttt tgtttttcat ttgccctatt ttgatcaata taaagacaca ccacaattat 1981 ccccatcaca ttctgataca cttttcaggg gcttgtgctc ccattgctgt gctcccataa 2041 aatcactgaa attataggtg aaatgaagga ctctgtgtca ggaaaggaac agggaaagca 2101 ttacagatgg ttccctgaag actttagctc aaagttggct tttgccaaaa aggccaaaga 2161 ggtggtatca taatgaaggt gattggaaac aaaagacaga accttttccc acccttgcat 2221 aaagccttgt aactgaacgc atgcctggtc actggctctt aggaagaatg tcatgggcct 2281 agagtagggt cagagaatga ttggcagaat ggggagccag gggttgctgg tgagggcaaa 2341 tttcaaagtt tagtcctctg gaaggtgaga caggatggaa atctacagca taataaaaga 2401 cctagatatg cagaagaatt gcttgctccc caaatcttga aactggagat gagggggcct 2461 tttagaactg gagtgatgca agctcaggat gaatagaaaa atgttctttg gcacacagtg 2521 ggcagcatac ttccagagct catttccaga gagaagatag aggcaggaag tagaaatgga 2581 atccttcagg gtttggaagg atcagcacat gtcagagtca caaatggcta cagggtggag 2641 ggagtgaggc atggaaagag cagaactaga tggtttgaat ctcttcaaat ctcttcaaac 2701 tgtgtaacct tgcatcctgc cttcttagag gtgtaatggg gataggcact ccagccatgc 2761 agggcagttg tgcgagttaa atgaaattat gtctgtgaag gccctctgca aatggaggag 2821 catcatataa ataagaagca ctgctattat tatgcccaag ctgcagaatc tttgtttgga 2881 aaagtggggc tgagacttag agcagcctaa tctcatccct gatgcagcca taggagggat 2941 cttaatgaca gacgggccac cctatcgcgg acttcagatg ctggccacta cccctgccgc 3001 tgtctgagaa ctctggggag ctccccaaat gctcagcctg tctaccaggc caaatatctc 3061 tgcctgcctc ctgctcaggg atgggaacat tggcccagga ctcaggttca ggacgggagg 3121 gcaaacgcag caggctagag ctgacttgtg gtgactgaag gatggggcca gtagaatctt 3181 ttcttctcca ttcacactaa atgatgcctc ctagactaac ctgaaggatc cattaggtcc 3241 tgagctttgg ggtgtctagg gtgtccatgg ccactgccag ggactcatga aaagaatgat 3301 ggggaggaaa aggaaaagat catcagtggg gtaagagttg ggggaagaca gagaggaata 3361 actttcaaaa ctctatctct cctttcccat tgtcctgccc cggggccttt tttacacctt 3421 tcagttaata gtttctcctt tcctatttct tccctgccca gggaggattg gaacagagac 3481 aaaagggagg agagacggac agcgacaagt ggagaaaatc ggcgaaactt gagtggcaga 3541 gaagtctgag cgctgagacc cggcggcccc gtgcgccttc ccacctggcg ccgatccact 3601 ttcctcgggg tagcggccca acccacttcg ctgccagccg atccctttta cccgtggcta 3661 ccgggaccac tctactctcg cccacttggc tctgcctaag cgtcctagcc ggagcgcggt 3721 ctctgccacg tggggagggg cgcggccgag ttgctgaaga gcgcttctga ttggccagag 3781 ggcggggttc ttggcgtctc gccggccaga cccctccctc aaaggcgggg cctggagatc 3841 cacagctgga aagggcggag ccccagcagg gcagctggaa aggggcgggg cctgacgcgc 3901 gcggctcgcc gcggcgggct gggggcgccc tggtctgcca taaagtgaat gggcgccggc 3961 tgggggtggc agtacgcggt gaggctcact ccctccgaga gtccaggagc gcccgagcgg 4021 agaggcggcc cgggagcagg ggggcggccc ccactccggc cgggtgcccg gcccctggcc 4081 cctgcctgcc ctctagatcg ccgccgcagc cgccgctact gggagtctgg cgcgccgaag 4141 ttcctattct tcaaaaggta taggaacttc cgccaccatg gtgagcaagg gcgaggagct 4201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 4261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 4321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 4381 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 4441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 4501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 4561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 4621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 4681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 4741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 4801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 4861 cgggatcact ctcggcatgg acgagctgta caagttattt ctgaactgct gcccgggctg 4921 ctgcatggaa ccggatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt 4981 agatccaaaa aagaagagaa aggtaatata agaagttcct attctctaga aagtatagga 5041 acttcagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact 5101 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 5161 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 5221 tttcacaaat aaagcatttt tttcactgcg aattctcgta aaactcttca tggcagtagt 5281 tattattctc tctctctctc tttttctttt ttcttgagac aggatatttc tctgttgccc 5341 aggctggagt gcagtggcac agtcttggct cactgcagcc tggacctcct gggctcaagc 5401 catcctccca cctcagcctc ccaagtagct ggggctacag gcacatggcc accaggccca 5461 gataattttt catttttgta gagactgagt ctcaccatgt tacccaggtt tattattctc 5521 attttttaga tgaagagact gaggtccaga gaagctcaat gacttgccta gttttacaaa 5581 tctcctgcca tcacataccc ctcagcgtcc ttaataagag ggaggccacc aactatgtgc 5641 tgggcactgt ggtggatgct ggagctatag ggttgagtat ataagaaatg gtgttgctgg 5701 agcaactgtt gcttgcttac ctgacctatc tgagaattaa ttagcagggg aacatatttt 5761 tgttttcaga ttcaatataa gaacttgtgt gggcaaaaat aaagatcagt agtaataaca 5821 gtagttccca tttgctgact gtactgtcct aagtgcatat atatatacat acacacacgc 5881 atacctatac tcctctaata ctcaaaatga tcctgtttat gtattgttaa tatgctcatt 5941 ttatttttaa atttttattt atttttattt ttatttattt ttgagacgga gtctcattct 6001 gtcgcggagg ctgaagtgca gtggtgcgat ctcagctcag tgcgacctcc gcctcccggg 6061 ttcaagtgat tctcctgcct cagcctccgg actagctggg attacaggcg cccgcctcca 6121 cgcccagcta atttttgtat ttttagtaga gatggggttt cgccatgttg gccaggctgg 6181 tctcgtactc ctgaccttga gtgatccacc tgcctcggcc tcccaaagtg ctgggattac 6241 aggcatgagc caccgcgccg ggctaatatg ctcattttag tgaggcaaaa atagaggctc 6301 agagtctgat ttgtacaaaa ctacagagca gttaagtgtc ctctcagatg tgtaccctga 6361 tctgggtgac tctaggactc taggtctcaa ctgttacaac cagttaaggg tttggggaag 6421 cactgggcca agagtcagga aaatggaagc cacaggtagt gcaaggtctt gggaatggga 6481 cgtctggtcc aaggattcac gcgatgactg gaacccgaag agccggggcc cggtttacgg 6541 ccgccatgaa gcaacgcgcg ccggtaggtt tgggaatcag ggagccctct gaataggaga 6601 ctgagttggg agggaaaggg gcttcgctgg gggagcctcg gcttcttctg ggagaaaatt 6661 cccacggcta cctagtgagc ctgcaaactg gtaggcgccg gcgtaggcgc gcgggcgggg 6721 ccgggggcgg ggcctgcggg gcgtggcggg gcgggcagag ggcggggcct gcttctcctc 6781 agcttcaggc ggctgcgacg agccctcagg cgaacctctc ggctttcccg cgcggcgccg 6841 cctcttgctg cgcctccgcc tcctcctctg ctccgccacc ggcttcctcc tcctgagcag 6901 tcagcccgcg cgccggccgg ctccgttatg gcgacccgca gccctggcgt cgtggtgagc 6961 agctcggcct gccggccctg gccggttcag gcccacgcgg caggtggcgg ccgggccctg 7021 aggcgcggga tcgatccatg acaccccccc gccccccata tctcactata attcactgtc 7081 aaatggcttt tatgatttgg agtttgggga tacttttaaa gacagggtct tggtagctcc 7141 tagatggctt gggattcact tagtaaccct tgctggccta aaattcagga acttctacct 7201 catcttccca agtactgggt tagtagggat gtccagcata tttagcttgc tttaatgatt 7261 ttgctgtact tttttctgtg tgtacaggtg catgtatatt tataaaacta acataatata 7321 ttttaccccc aaagtaccat aaccctaatt aatgctatta aatgttttgt atgctgggct 7381 ctgggcatac taatataacc aatattatac aatgcaaaac aattttggaa tatcttctgt 7441 aaaattatct tattagtaag ttgtaaccat ctcagacttc taagtttgag tttgtaaagg 7501 atcagttttt tttggtgacc tacacacaca cacacacaca cacacacaca cacacacaca 7561 ggggggtgga ttctgagaat ttagagaatt tagatggtta ataccagttt ttatttttat 7621 tctgtgtgat atttatttat taagatttat ttatttatta catgtaggta cactgtatct 7681 gtcttcagac acaccagaag agggcatcag atctcgttac agatggttgt gagtcaccat 7741 gtggttgctg ggatttgaac tcaggacctc tggaagaaca gtcagtgctc ttaaccgttg 7801 agccatctca ccagccccgt gatattttat ttaacttact agaatatact gtgttaaaac 7861 atattttagg ggattgggga gatggcccat tctgtaaagt gtctgccatg tcatcttcag 7921 gacctgcgtt tgtaacccca gcatggagaa gacagagaca gggagacccc tgggcttcct 7981 ggcaagctag actagcaaat ttggttaact aggttcaatg agagactcag tctcaaaaca 8041 taagaagtga attttttaga cccaatttca atctctgaac tccatacgta tgcacatgta 8101 cctgcatata aatatatata cacacccata aacacatgca cacacaaaaa atggttcaaa 8161 agttcttaaa atgtataaga gtagaaagtg tagagccggg agtggtggcg cacgccttta 8221 atcccagcac ttgggagcag aggcaggtgg atttctgagt tcgaggccag cctggtctac 8281 agagtgagtt ccaggacagc cagagctata cagagaaatc ctgcctcaaa aaaccaaaaa 8341 taaagtgtat atactaatct ttcatgccta aaatatttat ttttgccaca tttttcatac 8401 ctctttaata tttttctttt tctctttgtg cttcaaagga aattttgtgt catgttttac 8461 ttttcattct tgactctatt cagcagtaag acgcagcatg gctttcttag agaagtataa 8521 tctattacaa aacggtaaca tttaactgct ctacataccc tcttgtagct aaattcctct 8581 gttaaactaa gacttattat ctaaacgtaa ttaaatgttg atatcttata atgctccact 8641 ttgaaacagc tgtctttctt ttgcagatta gcgatgatga accaggttat gacctagatt 8701 tgttttgtat acctaatcat tatgccgagg atttggaaaa agtgtttatt cctcatggac 8761 tgattatgga caggttagta agacctcgat tgaagttatt tttagagggt gtgtgtgtgg 8821 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgta agagagagag gagaggcatg 8881 tgtgatatat atgtgaagtg tattgcatgt acatggaatc cagagaagga cattaggtgt 8941 cctactctgt tgctctctta ttcccttgaa tcagtgtctc ttgctgaacc tggggcatac 9001 atactgattt ggctaggctg cctggctagc aagacccagg gatctgcttg tctctgcccc 9061 ccagtgctga cgttacaagc aggtgcaacc acatctggtt tttatatggg tactggggga 9121 tctgaactta gggcctgatg cgtgcccaag tgctctcacc cgccaagcca cctctccagc 9181 caaagggttt tttttcttgt attaattcta acttagcaac tgttgagtag ttgatgtttg 9241 aatataagct aagccaactt attagaataa atgttttcta ttttttactt gtatgtgtga 9301 gtgcctgcat gtatgtatgt gaagcatatg tattccagga tgtcagaatt gtgatggatt 9361 ccctggaact ggagttacag accattgtga accactgtgt atgggtgctg ggaactgaac 9421 tttaggtcct ctgcaaaaac agtaaggatc ttaactgata aactatcttt ctagccctaa 9481 ggctttctac ttttaaagtt cgtttctctt tctctctctc tctctctctc tctctctctc 9541 tctccctctc cctccctcct cctcctcctc ctctttcccc cctctcgcct cctctttctc 9601 tctcccctcc ctctctctct ctcacacaca cacacagaca gagagaggag agagaaagga 9661 agccttacta attaattacc tagttttaga cataactagt gggaatccat agtaagtaaa 9721 tcaacattct tgaagagagg ttagaagaat tggctctcgt tgtccatcga ggctgtataa 9781 gattattctt ctgttttctc agcttaccat acatattttc tacgtagaca ataccaattc 9841 aaatacactg tacgtaaagt atatgcgcac acacacacac acacacacac acacacacac 9901 acaagttaca tattgtcata tagtactaaa atttatggac tttttcatat taatacatct 9961 agagctatca tgttttttat cttttaaaaa ttactttacg gggctacaga gatgtctcag 10021 cagttaggag cactgactgc tcttccagag gtcctgagtt caattcccag caaccacatg 10081 gtggctcaca accatctgta atgggatctg atccactctt atggtgtgtc tgaagacagt 10141 gacagtgtac tcatatacat gaaataaatc tttaaaaaat tactttacca tcagggtata 10201 tatatatata tatatgtgtg tgtgtgtgtg tgtgtgtatg aagtatttgc tatttgagag 10261 ggtctcactg tttagctcag tctggcttgg aacgttctgt atagcccaga ctggcctcaa 10321 ctcagcagtc cccctgaaag gctctcatat gtggagatta caggtatgtg ctactgtgcc 10381 cagccctaca tgttactttg tttttgaaat agggtatcaa atagcttaga ctggcttcta 10441 agtcagtgtg tagctaagga tggtctgagc ttctgaacct gctacttcaa ctcctggtgt 10501 gccatcatgg ccagtttgca cagttgtgag agtagaactc aggacctcct atgtgttact 10561 agacatgttc tctaccagct gagctacatc ccaattcgcc tacatttact tttatttttt 10621 aaaagattta tttatgtata cagcactctg cctgcatgta catctgcatg ccagaagagg 10681 gcaccagatc tcatcataca tagttgtgag ccaccatgtg gttgctgggg attgaactca 10741 gggcctctgg aagagcagtc agtgctctta acctctgagc catctctgta gccccctaca 10801 tttattttaa agattatagg ctattctatt catagatgtg ccataactga tttaaccaga 10861 tcttcattat tttgtactta gtagagtata ttagagggaa tgaatcttaa atacacattt 10921 tgacacttgc tcagctttag acaagtaaaa atcactggtc aaggaaatgg tgcttagtgg 10981 atgcttctga actgtcaaat tgtcctccaa caagttacat cattttatac ttccaacgtt 11041 atgtgagagt tcatttttct ttaggctctt ctagtatttt atatttgaga aataaaattt 11101 aaccaaaatt tgccaatcta gtaagcttgt ttttatttgt atctttaaat tttatgatag 11161 atgttatagt gtactctcct ctcccctccc ctcccctctc ctcctcagca ggatcttgtt 11221 atgtagccct aagggatcta atacttactc tgtagtccag gctgtccttc agctcatgag 11281 acacaccatc atgcttggca tgtttcttgt tctgaaaggt atctggtttt aattttggtt 11341 gagctggtgt gttggtgtat atctgtaatc tcagcacagg caagtagaga caaggattat 11401 gattacagtc cagcctgggc tatttagagg gattctcttt aatctttcct catgccccaa 11461 aatcttacct ttggtatatg aaaaatagtc tccacttctg caaaatattg ctttatgaag 11521 taagaattaa ttcccttcat agagacaagg aatgtgtcct gtaaaagttt aatgtgtaag 11581 aagtatttgt tataaaagat aaatattcag aatcttcttt ttaattcctg attttatttc 11641 tataggactg aaagacttgc tcgagatgtc atgaaggaga tgggaggcca tcacattgtg 11701 gccctctgtg tgctcaaggg gggctataag ttctttgctg acctgctgga ttacattaaa 11761 gcactgaata gaaatagtga tagatccatt cctatgactg tagattttat cagactgaag 11821 agctactgtg taagtataat taacttataa ttaaaaaaat agggccattc tagttttatc 11881 tatatttttt ttaaacttgt gcaaactatg ctacataatc aacttacttg attatgtaat 11941 atatatagta atagttggaa ttactattat tacatattta tttttctgtg ctggggtaga 12001 acccagggaa ctaagtactg taataaccga gcttcattca tcctagtcat ttttaatttt 12061 tttttttttt tttgtttttt cgagacaagg tttctctgtg tagccctggc tgtcctggaa 12121 ctcactctgt agaccaggct ggcctcgaac tcagaaatct gcctgcctct gcctcccagt 12181 gctgagatta atgacgtgca ccaccactgc ctggcatttt tttaaagttt taatttgaga 12241 ccctttctca ctaacttgct cacactatcc tcgaactcat tacgtgggct ggtctagaaa 12301 tttcagtcaa cttgcctcag cttctcaagt agctgggatt acaggcaggt gctaccactt 12361 ctggccatga tcattgttgt tgttgctatt attattatta ttatttcact gatacttcat 12421 atcacaacac agaatagtaa ctgtagttca tgaccaacaa aaaagttgta cagtttctct 12481 gttgtcttat tttgatctgg attttgattt gtttcatggc actgggcagg ctaatctcaa 12541 actgcatggc tcaaatagtc cttctgtctt aatctcccat gttctggaag tataggtatc 12601 actacaccta gctgtatttt catattttaa aaaaaacgcc taagaattgc tattgaatga 12661 ggcggagaga tggctcagca gtcacaagta cttgtagagg actggggttt ggttactagc 12721 acctcaaaga tggcttacaa ttatctgtag ctccaattcc agaggatctg tgtggtaaag 12781 ctgcatgtat atgggcatgc aggtaaaact gctaaacata taaaaatagc tcagtcttaa 12841 gagaattggt cgactttttc gccctttgac gttggagtcc acgttcttta atagtggact 12901 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 12961 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 13021 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 13081 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 13141 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 13201 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 13261 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 13321 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 13381 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 13441 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 13501 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 13561 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 13621 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 13681 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 13741 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 13801 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 13861 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 13921 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 13981 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 14041 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 14101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 14161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 14221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accaggtcga 14281 gtagggataa cagggtaatg gatcctcgtt gggcacctgc actcatgtac gcaggcccac 14341 atatagatgt acacacgcat atcattcaaa atagaaatta tttatacaag tagtgcgttg 14401 gagagctggc tcaggagttg ttaatgcttg ctacccttgc aaaggaccca agttcaattt 14461 ccagcacctg tgttgggcat ctcacaacca catgtaaatc tagtttcagg ggatttcacc 14521 cctcttctga actcttcagg cacttatacc cacaaatgga catctccaca tagagacaca 14581 tacttttaaa aaagtaatct agggtctaga gaaatggtta tagaccacgg actgggatca 14641 gttcccagaa gccacatcct gtggctcaca attgcctgtg aatcaagttc tagatctggt 14701 ggcctcgtct agcctgcagt catcaacaaa cacatgcaca catccataca cacataattt 14761 aaaagaaagc gaaagtaacc tataatgcta aacaaagcac attctcagtg tagactgcag 14821 tggtgatgaa ccttgaaaat atattaatag aagccagaca cgtaagtcac aaatggtata 14881 actcaattta tatgaaattc ccagagagtc caaagttaaa aaaaacaaaa aggcagatta 14941 ttggttgtca gtagctacag agatgggaca ttgaagatat taataggtat agggttgagg 15001 attcatggcc tttccctctt ccatgttagc atgtctattg ttgttaacct tattcaactc 15061 atgttagggc aatcatattg atgagactta atgagtatag cttgacattc ctagaagaca 15121 gagtctcaca tcagactctc tgatcctctg actcttagaa tctttcaaca ccctcttctg 15181 aaatgatctc tgagcctttg gttgtgactg aattatgaga aagaatttta tgttgaatga 15241 attttacttc agcattttta caaaactgaa gctggggatg gcgtttcatg cgtgcagtcc 15301 ctaccatttg cctataagcc tggaggaggc agaagtagaa ttaggctttt cagggacatc 15361 ttcagctgca tcatgagctt gaggacaact tgagctacat aagattccca tccccacccc 15421 cacacacaca cacacatcta gtatgtacct agtatgcagt cttttcatat gtttaaaatg 15481 tagaaaggta tatgtgaagt ctcccttttc cccacaactt agtccctcca aagtattgca 15541 tcagtttcct tgtacatgat tttatgcata tataagatac actatttctc tctctctctc 15601 tctccttccc tccttccctc caacctctgg gtatgagttg agggttatgt atgtttcaag 15661 ttgtaataag aattgccact caaacatttc atcccatttt aagttaagtt tgtctgattt 15721 tccactttag aagggcagcc atgatggctc atgcctataa tcccatccta gcacttgtaa 15781 gcctaataca taagactgga ggattgtcat cagcttgagg ctagcctggt ctacctaatg 15841 agttccagcc agccaagagt agaatataag accctgtctc caaaaaatgt accaaagggg 15901 ctggaggggt aactcagtgg ttaagaacag tagctgctct tccagaggac ctgggttcaa 15961 ttcccagcac ccacatggca gcttgcaact atctgtaact gtccagcttt gggatatccg 16021 acaccattat accaattaca taaaatgaaa aaattgtaaa atgtgctaaa atgagtgggg 16081 agggggagca tggtgtggtt ctgtgggctt gggattttgc catgaatcca tgactatatc 16141 tcagcaatat gtggattatt tctggaaacc tgtggggcat gggtgtatgg gaagagggga 16201 gagagagaga gcccgcatcc agccagagtt cctgtgctct gggcaggcgg acgtgggagg 16261 actgctggac gatttccact tggccctggg tgggcatctg gctgtgtgaa gccactgacc 16321 ccactcagcg gggggtagat aaggggcatt tcccatacca ggttctcaga ctccagcatt 16381 ccacgctgga tacagcagaa gagctgagaa cagaataggg gccccaggga gacactctcg 16441 tcccagagat aaagaaggca gagggagaga gattcctacg caggcgagag gccttagtct 16501 gggccttgat gaacagagac ggtctatggt tttagagctt tattgtagaa aggcaggggg 16561 aaagagagaa ggtagaaaga gagagaccag ccatggccaa gaggagagaa ggggggaaag 16621 gagagagaga agaaaggcta gagagtaaga aaggttaaag agacagaagt aaaagcttag 16681 agtaagagag agagagagag caagagggag agtaagaaaa gtaaaagctt aagagagcga 16741 ggaggggcca gcccctctaa tagtgggctt gttatcttgt tgttgctagg taactgtgaa 16801 gcgtctagcc tgaaggccag aagcttggga cattgtctat gtgactgaaa gccatgagcc 16861 tctactgtgg gggctgtgga ggtggtaact tggacaggag ccaggggttc caggagacat 16921 gagggaatgc cttctgtccc atgtaggtag gaattaccac caccggcttt caccgtagtt 16981 cactggagac tgtttgtaca tagcccattg ccccacagaa accaggcact cactgtttca 17041 tttgcataag aattagaagg ctttattttg gctgggattg gtgacttgcc ccagaaattc 17101 cagcttttgg gaagccgaat cctggagaac caggagttca aggtcatcct caggtacctc 17161 atttgttcaa gaccagcctg tgctaccatg agaccttgtc cctactcccc tcaaccccaa 17221 aacacgctgt tttcatttta taggttgtac taaccatgga ggatggtcat ttgcctttct 17281 cttgatttca gaagcgtagc gcgggctttg caccatgatc tagtgtttcc agataggctt 17341 gcctctccac agccaggctt atcctgcttc agtcccatgt ttggcaaggg caacatatga 17401 ctgaagaact tgcatagcct tatggtgtat catgactctt agccccagtt tataactgtc 17461 taacagctgg tggcttttgt gaaaaaggaa agcatcattt gaacttttaa cttttcagct 17521 gtttgttttg actttgcata aatactgaga ctagctagga aatgagcttt tgtttcattt 17581 gctaaaaatg attttttttt catgaggcaa tttcaagcaa tttgaattta gcaatctcta 17641 cttgtctgaa aagaagttat taattaaaaa aaaacaaaca aacaggaacc tattatgctg 17701 gctagtcaca tgaaagaatc caaatgaaca cagtcaaggc tgaatcaaca catgaaatta 17761 ttttctaatt ccccaaatta catttatgct aattagtatg tagtaaaaca ttttaattgt 17821 ttccaatgtt aaacattaca gaaataattt tgatgttttc ctaataaccc agcctttgat 17881 catgtttgca tttatttgat tgttgtcagt ttattaaata attaagtaag agcaacaaga 17941 taaaatgact tcatgagagt ccttttctgt aacttagaac gtcagtagtc ataggaactg 18001 cggtcgttcc cttacctttc agaagtaggc taaagagttg aacgcaaagg tggtttttcg 18061 aatcttcctg atccttaagc ccccactagg tttatattct atatttgata tcggattcga 18121 acccctcgag cttaggatcc ggaaccctta atataactaa gttattaggt ccctcgaaga 18181 ggttcactag tactggccat tgcggccgct tcgaggctgc ctcgcgcgtt tcggtgatga 18241 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 18301 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc 18361 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca 18421 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 18481 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 18541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 18601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 18661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 18721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 18781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 18841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 18901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 18961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 19021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 19081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 19141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 19201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 19261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 19321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 19381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 19441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 19501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 19561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 19621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 19681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 19741 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 19801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 19861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 19921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 19981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 20041 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 20101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 20161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 20221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 20281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 20341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 20401 gttccgcgca catttccccg aaaagtgcca cctgacgg //