LOCUS Ple30\(pEMS1158) 17270 bp DNA circular 26-JAN-2009 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2364 5'- GCGTATCACGAGGCCCTTTC -3' Tm= 56.0 Deg C Antisense primer: oEMS2611 5'- CAGGGCAAAGTCAACAGTGTCAC -3' Tm=56.0 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 727 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|508254700| COMMENT VNTDBDATE|508254700| COMMENT LSOWNER| COMMENT VNTNAME|Ple30 (pEMS1158)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#206=(AnlzFontBackColorItem 736 758 43633766))) (CObList) 17498167 COMMENT (CStringList) 0 851972 (CObList))) FEATURES Location/Qualifiers misc_recomb 11133..14944 /vntifkey="86" /label=5'\homology\sequence misc_marker complement(16043..17264) /vntifkey="22" /label=AmpR misc_marker 9682..11107 /vntifkey="22" /label=AmpR misc_recomb 6041..9686 /vntifkey="86" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 2087..6040 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 11114..11131 /vntifkey="21" /label=I-Sce\I misc_feature 969..1002 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 1003..1013 /vntifkey="21" /label=Kozak\consensus misc_feature 1010..1729 /vntifkey="21" /label=EGFP misc_feature 1730..1765 /vntifkey="21" /label=Tetracysteine\residue misc_feature 1766..1838 /vntifkey="21" /label=NLS misc_feature 1844..1877 /vntifkey="21" /label=MFRT misc_feature 2031..2081 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..960 /vntifkey="21" /label=Ple30\(CD68-A) /note="1306" primer 54..73 /vntifkey="27" /label=oEMS2364 primer 737..759 /vntifkey="27" /label=oEMS2611 misc_feature 139..502 /vntifkey="21" /label=Region\1 misc_feature 503..585 /vntifkey="21" /label=Region\2\(upside\down) misc_feature 586..960 /vntifkey="21" /label=Prom\region BASE COUNT 4614 a 3889 c 3882 g 4885 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccag ggatgatgag tataatccaa ggaccagaga agtcttctct 181 gatcaccacc ttgggaggaa gacatgggtg ccctaacact ctcgagacct gctgggttaa 241 ttaaaagcta tttcttaccc aaacgtaacc attgcttcct ccacccattt cctgagtcaa 301 atgggaaagc tgttgggtga agcctggctg gctgggcaag tttgactgtg ttctgaataa 361 gcaccttcac tatgggctaa gagatccctt ggtgtggggg tgatcttaca gtagtcagag 421 cagatggaca gtccttttca cccttgctta atagccagag ctgtttcatg cctggggcac 481 acacaattct aatgctggac ttcttttggc agagaggaga tggttagcaa cccagggcag 541 gctccctccc aggggccccc tcccctcagc ctccttcctc cttacgaaag gagtggaaac 601 cgatttcccc accaagggag gggcctgtac ctcagctgtt cccatagcta cttgccacaa 661 ctgccaagca agtttcgctg agtttgacac atggatccct gtggatcaac tgccctagga 721 ctccgtttgc acccatgtga cactgttgac tttgccctga tgaagcaggg ccaacagtcc 781 cctaacttaa ttacaaaaac taatgactaa gagagaggtg gctagagctg aggcccctga 841 gtcaggctgt gggtgggatc atctccagta caggaagtga gactttcatt tcctcctttc 901 caagagaggg ctgagggagc agggttgagc aactggtgca gacagcctag ctggactttg 961 ggcgcgccga agttcctatt cttcaaaagg tataggaact tccgccacca tggtgagcaa 1021 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 1081 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 1141 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 1201 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt 1261 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 1321 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 1381 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 1441 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 1501 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 1561 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac 1621 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 1681 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagttat ttctgaactg 1741 ctgcccgggc tgctgcatgg aaccggatcc aaaaaagaag agaaaggtag atccaaaaaa 1801 gaagagaaag gtagatccaa aaaagaagag aaaggtaata taagaagttc ctattctcta 1861 gaaagtatag gaacttcagc ggccgcgact ctagatcata atcagccata ccacatttgt 1921 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 1981 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 2041 tagcatcaca aatttcacaa ataaagcatt tttttcactg cgaattctcg taaaactctt 2101 catggcagta gttattattc tctctctctc tctttttctt ttttcttgag acaggatatt 2161 tctctgttgc ccaggctgga gtgcagtggc acagtcttgg ctcactgcag cctggacctc 2221 ctgggctcaa gccatcctcc cacctcagcc tcccaagtag ctggggctac aggcacatgg 2281 ccaccaggcc cagataattt ttcatttttg tagagactga gtctcaccat gttacccagg 2341 tttattattc tcatttttta gatgaagaga ctgaggtcca gagaagctca atgacttgcc 2401 tagttttaca aatctcctgc catcacatac ccctcagcgt ccttaataag agggaggcca 2461 ccaactatgt gctgggcact gtggtggatg ctggagctat agggttgagt atataagaaa 2521 tggtgttgct ggagcaactg ttgcttgctt acctgaccta tctgagaatt aattagcagg 2581 ggaacatatt tttgttttca gattcaatat aagaacttgt gtgggcaaaa ataaagatca 2641 gtagtaataa cagtagttcc catttgctga ctgtactgtc ctaagtgcat atatatatac 2701 atacacacac gcatacctat actcctctaa tactcaaaat gatcctgttt atgtattgtt 2761 aatatgctca ttttattttt aaatttttat ttatttttat ttttatttat ttttgagacg 2821 gagtctcatt ctgtcgcgga ggctgaagtg cagtggtgcg atctcagctc agtgcgacct 2881 ccgcctcccg ggttcaagtg attctcctgc ctcagcctcc ggactagctg ggattacagg 2941 cgcccgcctc cacgcccagc taatttttgt atttttagta gagatggggt ttcgccatgt 3001 tggccaggct ggtctcgtac tcctgacctt gagtgatcca cctgcctcgg cctcccaaag 3061 tgctgggatt acaggcatga gccaccgcgc cgggctaata tgctcatttt agtgaggcaa 3121 aaatagaggc tcagagtctg atttgtacaa aactacagag cagttaagtg tcctctcaga 3181 tgtgtaccct gatctgggtg actctaggac tctaggtctc aactgttaca accagttaag 3241 ggtttgggga agcactgggc caagagtcag gaaaatggaa gccacaggta gtgcaaggtc 3301 ttgggaatgg gacgtctggt ccaaggattc acgcgatgac tggaacccga agagccgggg 3361 cccggtttac ggccgccatg aagcaacgcg cgccggtagg tttgggaatc agggagccct 3421 ctgaatagga gactgagttg ggagggaaag gggcttcgct gggggagcct cggcttcttc 3481 tgggagaaaa ttcccacggc tacctagtga gcctgcaaac tggtaggcgc cggcgtaggc 3541 gcgcgggcgg ggccgggggc ggggcctgcg gggcgtggcg gggcgggcag agggcggggc 3601 ctgcttctcc tcagcttcag gcggctgcga cgagccctca ggcgaacctc tcggctttcc 3661 cgcgcggcgc cgcctcttgc tgcgcctccg cctcctcctc tgctccgcca ccggcttcct 3721 cctcctgagc agtcagcccg cgcgccggcc ggctccgtta tggcgacccg cagccctggc 3781 gtcgtggtga gcagctcggc ctgccggccc tggccggttc aggcccacgc ggcaggtggc 3841 ggccgggccc tgaggcgcgg gatcgatcca tgacaccccc ccgcccccca tatctcacta 3901 taattcactg tcaaatggct tttatgattt ggagtttggg gatactttta aagacagggt 3961 cttggtagct cctagatggc ttgggattca cttagtaacc cttgctggcc taaaattcag 4021 gaacttctac ctcatcttcc caagtactgg gttagtaggg atgtccagca tatttagctt 4081 gctttaatga ttttgctgta cttttttctg tgtgtacagg tgcatgtata tttataaaac 4141 taacataata tattttaccc ccaaagtacc ataaccctaa ttaatgctat taaatgtttt 4201 gtatgctggg ctctgggcat actaatataa ccaatattat acaatgcaaa acaattttgg 4261 aatatcttct gtaaaattat cttattagta agttgtaacc atctcagact tctaagtttg 4321 agtttgtaaa ggatcagttt tttttggtga cctacacaca cacacacaca cacacacaca 4381 cacacacaca caggggggtg gattctgaga atttagagaa tttagatggt taataccagt 4441 ttttattttt attctgtgtg atatttattt attaagattt atttatttat tacatgtagg 4501 tacactgtat ctgtcttcag acacaccaga agagggcatc agatctcgtt acagatggtt 4561 gtgagtcacc atgtggttgc tgggatttga actcaggacc tctggaagaa cagtcagtgc 4621 tcttaaccgt tgagccatct caccagcccc gtgatatttt atttaactta ctagaatata 4681 ctgtgttaaa acatatttta ggggattggg gagatggccc attctgtaaa gtgtctgcca 4741 tgtcatcttc aggacctgcg tttgtaaccc cagcatggag aagacagaga cagggagacc 4801 cctgggcttc ctggcaagct agactagcaa atttggttaa ctaggttcaa tgagagactc 4861 agtctcaaaa cataagaagt gaatttttta gacccaattt caatctctga actccatacg 4921 tatgcacatg tacctgcata taaatatata tacacaccca taaacacatg cacacacaaa 4981 aaatggttca aaagttctta aaatgtataa gagtagaaag tgtagagccg ggagtggtgg 5041 cgcacgcctt taatcccagc acttgggagc agaggcaggt ggatttctga gttcgaggcc 5101 agcctggtct acagagtgag ttccaggaca gccagagcta tacagagaaa tcctgcctca 5161 aaaaaccaaa aataaagtgt atatactaat ctttcatgcc taaaatattt atttttgcca 5221 catttttcat acctctttaa tatttttctt tttctctttg tgcttcaaag gaaattttgt 5281 gtcatgtttt acttttcatt cttgactcta ttcagcagta agacgcagca tggctttctt 5341 agagaagtat aatctattac aaaacggtaa catttaactg ctctacatac cctcttgtag 5401 ctaaattcct ctgttaaact aagacttatt atctaaacgt aattaaatgt tgatatctta 5461 taatgctcca ctttgaaaca gctgtctttc ttttgcagat tagcgatgat gaaccaggtt 5521 atgacctaga tttgttttgt atacctaatc attatgccga ggatttggaa aaagtgttta 5581 ttcctcatgg actgattatg gacaggttag taagacctcg attgaagtta tttttagagg 5641 gtgtgtgtgt ggtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg taagagagag 5701 aggagaggca tgtgtgatat atatgtgaag tgtattgcat gtacatggaa tccagagaag 5761 gacattaggt gtcctactct gttgctctct tattcccttg aatcagtgtc tcttgctgaa 5821 cctggggcat acatactgat ttggctaggc tgcctggcta gcaagaccca gggatctgct 5881 tgtctctgcc ccccagtgct gacgttacaa gcaggtgcaa ccacatctgg tttttatatg 5941 ggtactgggg gatctgaact tagggcctga tgcgtgccca agtgctctca cccgccaagc 6001 cacctctcca gccaaagggt tttttttctt gtattaattc taacttagca actgttgagt 6061 agttgatgtt tgaatataag ctaagccaac ttattagaat aaatgttttc tattttttac 6121 ttgtatgtgt gagtgcctgc atgtatgtat gtgaagcata tgtattccag gatgtcagaa 6181 ttgtgatgga ttccctggaa ctggagttac agaccattgt gaaccactgt gtatgggtgc 6241 tgggaactga actttaggtc ctctgcaaaa acagtaagga tcttaactga taaactatct 6301 ttctagccct aaggctttct acttttaaag ttcgtttctc tttctctctc tctctctctc 6361 tctctctctc tctctccctc tccctccctc ctcctcctcc tcctctttcc cccctctcgc 6421 ctcctctttc tctctcccct ccctctctct ctctcacaca cacacacaga cagagagagg 6481 agagagaaag gaagccttac taattaatta cctagtttta gacataacta gtgggaatcc 6541 atagtaagta aatcaacatt cttgaagaga ggttagaaga attggctctc gttgtccatc 6601 gaggctgtat aagattattc ttctgttttc tcagcttacc atacatattt tctacgtaga 6661 caataccaat tcaaatacac tgtacgtaaa gtatatgcgc acacacacac acacacacac 6721 acacacacac acacaagtta catattgtca tatagtacta aaatttatgg actttttcat 6781 attaatacat ctagagctat catgtttttt atcttttaaa aattacttta cggggctaca 6841 gagatgtctc agcagttagg agcactgact gctcttccag aggtcctgag ttcaattccc 6901 agcaaccaca tggtggctca caaccatctg taatgggatc tgatccactc ttatggtgtg 6961 tctgaagaca gtgacagtgt actcatatac atgaaataaa tctttaaaaa attactttac 7021 catcagggta tatatatata tatatatgtg tgtgtgtgtg tgtgtgtgta tgaagtattt 7081 gctatttgag agggtctcac tgtttagctc agtctggctt ggaacgttct gtatagccca 7141 gactggcctc aactcagcag tccccctgaa aggctctcat atgtggagat tacaggtatg 7201 tgctactgtg cccagcccta catgttactt tgtttttgaa atagggtatc aaatagctta 7261 gactggcttc taagtcagtg tgtagctaag gatggtctga gcttctgaac ctgctacttc 7321 aactcctggt gtgccatcat ggccagtttg cacagttgtg agagtagaac tcaggacctc 7381 ctatgtgtta ctagacatgt tctctaccag ctgagctaca tcccaattcg cctacattta 7441 cttttatttt ttaaaagatt tatttatgta tacagcactc tgcctgcatg tacatctgca 7501 tgccagaaga gggcaccaga tctcatcata catagttgtg agccaccatg tggttgctgg 7561 ggattgaact cagggcctct ggaagagcag tcagtgctct taacctctga gccatctctg 7621 tagcccccta catttatttt aaagattata ggctattcta ttcatagatg tgccataact 7681 gatttaacca gatcttcatt attttgtact tagtagagta tattagaggg aatgaatctt 7741 aaatacacat tttgacactt gctcagcttt agacaagtaa aaatcactgg tcaaggaaat 7801 ggtgcttagt ggatgcttct gaactgtcaa attgtcctcc aacaagttac atcattttat 7861 acttccaacg ttatgtgaga gttcattttt ctttaggctc ttctagtatt ttatatttga 7921 gaaataaaat ttaaccaaaa tttgccaatc tagtaagctt gtttttattt gtatctttaa 7981 attttatgat agatgttata gtgtactctc ctctcccctc ccctcccctc tcctcctcag 8041 caggatcttg ttatgtagcc ctaagggatc taatacttac tctgtagtcc aggctgtcct 8101 tcagctcatg agacacacca tcatgcttgg catgtttctt gttctgaaag gtatctggtt 8161 ttaattttgg ttgagctggt gtgttggtgt atatctgtaa tctcagcaca ggcaagtaga 8221 gacaaggatt atgattacag tccagcctgg gctatttaga gggattctct ttaatctttc 8281 ctcatgcccc aaaatcttac ctttggtata tgaaaaatag tctccacttc tgcaaaatat 8341 tgctttatga agtaagaatt aattcccttc atagagacaa ggaatgtgtc ctgtaaaagt 8401 ttaatgtgta agaagtattt gttataaaag ataaatattc agaatcttct ttttaattcc 8461 tgattttatt tctataggac tgaaagactt gctcgagatg tcatgaagga gatgggaggc 8521 catcacattg tggccctctg tgtgctcaag gggggctata agttctttgc tgacctgctg 8581 gattacatta aagcactgaa tagaaatagt gatagatcca ttcctatgac tgtagatttt 8641 atcagactga agagctactg tgtaagtata attaacttat aattaaaaaa atagggccat 8701 tctagtttta tctatatttt ttttaaactt gtgcaaacta tgctacataa tcaacttact 8761 tgattatgta atatatatag taatagttgg aattactatt attacatatt tatttttctg 8821 tgctggggta gaacccaggg aactaagtac tgtaataacc gagcttcatt catcctagtc 8881 atttttaatt tttttttttt tttttgtttt ttcgagacaa ggtttctctg tgtagccctg 8941 gctgtcctgg aactcactct gtagaccagg ctggcctcga actcagaaat ctgcctgcct 9001 ctgcctccca gtgctgagat taatgacgtg caccaccact gcctggcatt tttttaaagt 9061 tttaatttga gaccctttct cactaacttg ctcacactat cctcgaactc attacgtggg 9121 ctggtctaga aatttcagtc aacttgcctc agcttctcaa gtagctggga ttacaggcag 9181 gtgctaccac ttctggccat gatcattgtt gttgttgcta ttattattat tattatttca 9241 ctgatacttc atatcacaac acagaatagt aactgtagtt catgaccaac aaaaaagttg 9301 tacagtttct ctgttgtctt attttgatct ggattttgat ttgtttcatg gcactgggca 9361 ggctaatctc aaactgcatg gctcaaatag tccttctgtc ttaatctccc atgttctgga 9421 agtataggta tcactacacc tagctgtatt ttcatatttt aaaaaaaacg cctaagaatt 9481 gctattgaat gaggcggaga gatggctcag cagtcacaag tacttgtaga ggactggggt 9541 ttggttacta gcacctcaaa gatggcttac aattatctgt agctccaatt ccagaggatc 9601 tgtgtggtaa agctgcatgt atatgggcat gcaggtaaaa ctgctaaaca tataaaaata 9661 gctcagtctt aagagaattg gtcgactttt tcgccctttg acgttggagt ccacgttctt 9721 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 9781 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 9841 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 9901 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 9961 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 10021 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 10081 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 10141 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 10201 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 10261 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 10321 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 10381 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 10441 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 10501 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 10561 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 10621 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 10681 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 10741 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 10801 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 10861 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 10921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 10981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 11041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 11101 ctaccaggtc gagtagggat aacagggtaa tggatcctcg ttgggcacct gcactcatgt 11161 acgcaggccc acatatagat gtacacacgc atatcattca aaatagaaat tatttataca 11221 agtagtgcgt tggagagctg gctcaggagt tgttaatgct tgctaccctt gcaaaggacc 11281 caagttcaat ttccagcacc tgtgttgggc atctcacaac cacatgtaaa tctagtttca 11341 ggggatttca cccctcttct gaactcttca ggcacttata cccacaaatg gacatctcca 11401 catagagaca catactttta aaaaagtaat ctagggtcta gagaaatggt tatagaccac 11461 ggactgggat cagttcccag aagccacatc ctgtggctca caattgcctg tgaatcaagt 11521 tctagatctg gtggcctcgt ctagcctgca gtcatcaaca aacacatgca cacatccata 11581 cacacataat ttaaaagaaa gcgaaagtaa cctataatgc taaacaaagc acattctcag 11641 tgtagactgc agtggtgatg aaccttgaaa atatattaat agaagccaga cacgtaagtc 11701 acaaatggta taactcaatt tatatgaaat tcccagagag tccaaagtta aaaaaaacaa 11761 aaaggcagat tattggttgt cagtagctac agagatggga cattgaagat attaataggt 11821 atagggttga ggattcatgg cctttccctc ttccatgtta gcatgtctat tgttgttaac 11881 cttattcaac tcatgttagg gcaatcatat tgatgagact taatgagtat agcttgacat 11941 tcctagaaga cagagtctca catcagactc tctgatcctc tgactcttag aatctttcaa 12001 caccctcttc tgaaatgatc tctgagcctt tggttgtgac tgaattatga gaaagaattt 12061 tatgttgaat gaattttact tcagcatttt tacaaaactg aagctgggga tggcgtttca 12121 tgcgtgcagt ccctaccatt tgcctataag cctggaggag gcagaagtag aattaggctt 12181 ttcagggaca tcttcagctg catcatgagc ttgaggacaa cttgagctac ataagattcc 12241 catccccacc cccacacaca cacacacatc tagtatgtac ctagtatgca gtcttttcat 12301 atgtttaaaa tgtagaaagg tatatgtgaa gtctcccttt tccccacaac ttagtccctc 12361 caaagtattg catcagtttc cttgtacatg attttatgca tatataagat acactatttc 12421 tctctctctc tctctccttc cctccttccc tccaacctct gggtatgagt tgagggttat 12481 gtatgtttca agttgtaata agaattgcca ctcaaacatt tcatcccatt ttaagttaag 12541 tttgtctgat tttccacttt agaagggcag ccatgatggc tcatgcctat aatcccatcc 12601 tagcacttgt aagcctaata cataagactg gaggattgtc atcagcttga ggctagcctg 12661 gtctacctaa tgagttccag ccagccaaga gtagaatata agaccctgtc tccaaaaaat 12721 gtaccaaagg ggctggaggg gtaactcagt ggttaagaac agtagctgct cttccagagg 12781 acctgggttc aattcccagc acccacatgg cagcttgcaa ctatctgtaa ctgtccagct 12841 ttgggatatc cgacaccatt ataccaatta cataaaatga aaaaattgta aaatgtgcta 12901 aaatgagtgg ggagggggag catggtgtgg ttctgtgggc ttgggatttt gccatgaatc 12961 catgactata tctcagcaat atgtggatta tttctggaaa cctgtggggc atgggtgtat 13021 gggaagaggg gagagagaga gagcccgcat ccagccagag ttcctgtgct ctgggcaggc 13081 ggacgtggga ggactgctgg acgatttcca cttggccctg ggtgggcatc tggctgtgtg 13141 aagccactga ccccactcag cggggggtag ataaggggca tttcccatac caggttctca 13201 gactccagca ttccacgctg gatacagcag aagagctgag aacagaatag gggccccagg 13261 gagacactct cgtcccagag ataaagaagg cagagggaga gagattccta cgcaggcgag 13321 aggccttagt ctgggccttg atgaacagag acggtctatg gttttagagc tttattgtag 13381 aaaggcaggg ggaaagagag aaggtagaaa gagagagacc agccatggcc aagaggagag 13441 aaggggggaa aggagagaga gaagaaaggc tagagagtaa gaaaggttaa agagacagaa 13501 gtaaaagctt agagtaagag agagagagag agcaagaggg agagtaagaa aagtaaaagc 13561 ttaagagagc gaggaggggc cagcccctct aatagtgggc ttgttatctt gttgttgcta 13621 ggtaactgtg aagcgtctag cctgaaggcc agaagcttgg gacattgtct atgtgactga 13681 aagccatgag cctctactgt gggggctgtg gaggtggtaa cttggacagg agccaggggt 13741 tccaggagac atgagggaat gccttctgtc ccatgtaggt aggaattacc accaccggct 13801 ttcaccgtag ttcactggag actgtttgta catagcccat tgccccacag aaaccaggca 13861 ctcactgttt catttgcata agaattagaa ggctttattt tggctgggat tggtgacttg 13921 ccccagaaat tccagctttt gggaagccga atcctggaga accaggagtt caaggtcatc 13981 ctcaggtacc tcatttgttc aagaccagcc tgtgctacca tgagaccttg tccctactcc 14041 cctcaacccc aaaacacgct gttttcattt tataggttgt actaaccatg gaggatggtc 14101 atttgccttt ctcttgattt cagaagcgta gcgcgggctt tgcaccatga tctagtgttt 14161 ccagataggc ttgcctctcc acagccaggc ttatcctgct tcagtcccat gtttggcaag 14221 ggcaacatat gactgaagaa cttgcatagc cttatggtgt atcatgactc ttagccccag 14281 tttataactg tctaacagct ggtggctttt gtgaaaaagg aaagcatcat ttgaactttt 14341 aacttttcag ctgtttgttt tgactttgca taaatactga gactagctag gaaatgagct 14401 tttgtttcat ttgctaaaaa tgattttttt ttcatgaggc aatttcaagc aatttgaatt 14461 tagcaatctc tacttgtctg aaaagaagtt attaattaaa aaaaaacaaa caaacaggaa 14521 cctattatgc tggctagtca catgaaagaa tccaaatgaa cacagtcaag gctgaatcaa 14581 cacatgaaat tattttctaa ttccccaaat tacatttatg ctaattagta tgtagtaaaa 14641 cattttaatt gtttccaatg ttaaacatta cagaaataat tttgatgttt tcctaataac 14701 ccagcctttg atcatgtttg catttatttg attgttgtca gtttattaaa taattaagta 14761 agagcaacaa gataaaatga cttcatgaga gtccttttct gtaacttaga acgtcagtag 14821 tcataggaac tgcggtcgtt cccttacctt tcagaagtag gctaaagagt tgaacgcaaa 14881 ggtggttttt cgaatcttcc tgatccttaa gcccccacta ggtttatatt ctatatttga 14941 tatcggattc gaacccctcg agcttaggat ccggaaccct taatataact aagttattag 15001 gtccctcgaa gaggttcact agtactggcc attgcggccg cttcgaggct gcctcgcgcg 15061 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 15121 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 15181 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 15241 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 15301 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 15361 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 15421 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 15481 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 15541 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 15601 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 15661 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 15721 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 15781 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 15841 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 15901 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 15961 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 16021 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 16081 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 16141 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 16201 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 16261 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 16321 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 16381 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 16441 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 16501 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 16561 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 16621 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 16681 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 16741 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 16801 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 16861 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga 16921 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 16981 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 17041 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 17101 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 17161 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 17221 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgg //