LOCUS Ple178\(pEMS1090 18308 bp DNA circular 23-JAN-2009 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2412 5'- AATGATCCCCTAGTGTGCCAGC-3' Tm= 56.8 Deg C Antisense primer: oEMS2367 5'- GTGGAAGGAAAAGCGGCAAG-3' Tm=56.2 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 433 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|507990739| COMMENT VNTDBDATE|507991094| COMMENT LSOWNER| COMMENT VNTNAME|Ple178 (pEMS1090)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList)) COMMENT #193=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1023 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #194=(AnlzFontBackColorItem 420 1000 40304639) COMMENT #195=(AnlzFontBackColorItem 138 419 43633766))) (CObList) 1699618931 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers misc_feature 1010..1043 /vntifkey="21" /label=MFRT\(F5\mutant) misc_feature 1044..1055 /vntifkey="21" /label=Kozak\consensus\sequence misc_feature 1056..1772 /vntifkey="21" /label=EGFP misc_feature 1773..2798 /vntifkey="21" /label=Cre misc_feature 2799..2871 /vntifkey="21" /label=NLS misc_marker 2875..2908 /vntifkey="22" /label=MFRT misc_feature 3062..3112 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 3118..7071 /vntifkey="21" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 7072..10717 /vntifkey="21" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_feature 12171..15982 /vntifkey="21" /label=5'\homology\sequence misc_marker 10719..12145 /vntifkey="22" /label=AmpR misc_marker complement(17302..18302) /vntifkey="22" /label=AmpR misc_feature 12152..12169 /vntifkey="21" /label=I-Sce\I misc_feature 139..1001 /vntifkey="21" /label=Ple178\(RGS16-D) /note="1302" misc_feature 421..1001 /vntifkey="21" /label=Prom\region misc_feature 139..420 /vntifkey="21" /label=Region\4 primer 255..276 /vntifkey="27" /label=oEMS2412 primer 668..687 /vntifkey="27" /label=oEMS2367 BASE COUNT 4828 a 4192 c 4147 g 5141 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccga gtagggcctt ttgtgtctgt ctctgggtgg cacatgtgta 181 ctcatgtgct ttggagccag ccccaggact gctgagagcc tgggcagaat aatagtagta 241 gtaataataa taataatgat cccctagtgt gccagcctct attcaacata taaaggcagg 301 tcattgttca agaaaattat gttctaaaaa tagagccatt aattgcaatt gctttccttt 361 cttcttgtat tccctgctgt ccctccagct gacctatttc ctcttctttc ccgcaacccc 421 gggtacttat cagtataatc agatgctgtt ttgcccaggt ttgcaccctg tgcgcctgtc 481 ctcaggaccc agcaggctca gacttgcctg cgatcaaaga aagccctgtg tgcctcccag 541 acccaccacc cccgtgcaaa ctctctcaac acgacaccgc tgcgtgactt tcctgctgtt 601 actagaggtc ggcaggtact tttccctagg aaactgggtg tgcacgtctc atccccgtgc 661 caattctctt gccgcttttc cttccacacg cggcgtcctc tgggcggagg gtgcagctcc 721 gcgtgctcca gttgccgcga aagttgcacc cggcccagtc tggctgcggg ccccgcctcc 781 gccttctcag cgcccagccc caccctccga ttggtcagca gtgcccagcc ccgccccgct 841 cctccgaggg gatttaaagc agcggccgct cagtctgggc gcttgcaggc tgctaaaccc 901 aaccgcagtt gactagcacc tgctaccgcg cctttgcttc ctggcgcacg cggagcctcc 961 tggagcctgc caccatcctg cctactacgt gctgccctgc gggcgcgccg aagttcctat 1021 tcttcaaaag gtataggaac ttcgccgcca ccatggtgag caagggcgag gagctgttca 1081 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg 1141 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca 1201 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 1261 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc 1321 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc 1381 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 1441 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca 1501 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc 1561 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 1621 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca 1681 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga 1741 tcactctcgg catggacgag ctgtacaagt tatccaattt actgaccgta caccaaaatt 1801 tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt 1861 tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt 1921 cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg 1981 ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac 2041 atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca agtgacagca 2101 atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg 2161 caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa 2221 atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc 2281 tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg 2341 ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga 2401 aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag 2461 ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat 2521 ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat 2581 tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg 2641 cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc 2701 aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg 2761 aaacaggggc aatggtgcgc ctgctggaag atggcgatga tccaaaaaag aagagaaagg 2821 tagatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta ataagaagtt 2881 cctattctct agaaagtata ggaacttcag cggccgcgac tctagatcat aatcagccat 2941 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 3001 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 3061 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcgaattctc 3121 gtaaaactct tcatggcagt agttattatt ctctctctct ctctttttct tttttcttga 3181 gacaggatat ttctctgttg cccaggctgg agtgcagtgg cacagtcttg gctcactgca 3241 gcctggacct cctgggctca agccatcctc ccacctcagc ctcccaagta gctggggcta 3301 caggcacatg gccaccaggc ccagataatt tttcattttt gtagagactg agtctcacca 3361 tgttacccag gtttattatt ctcatttttt agatgaagag actgaggtcc agagaagctc 3421 aatgacttgc ctagttttac aaatctcctg ccatcacata cccctcagcg tccttaataa 3481 gagggaggcc accaactatg tgctgggcac tgtggtggat gctggagcta tagggttgag 3541 tatataagaa atggtgttgc tggagcaact gttgcttgct tacctgacct atctgagaat 3601 taattagcag gggaacatat ttttgttttc agattcaata taagaacttg tgtgggcaaa 3661 aataaagatc agtagtaata acagtagttc ccatttgctg actgtactgt cctaagtgca 3721 tatatatata catacacaca cgcataccta tactcctcta atactcaaaa tgatcctgtt 3781 tatgtattgt taatatgctc attttatttt taaattttta tttattttta tttttattta 3841 tttttgagac ggagtctcat tctgtcgcgg aggctgaagt gcagtggtgc gatctcagct 3901 cagtgcgacc tccgcctccc gggttcaagt gattctcctg cctcagcctc cggactagct 3961 gggattacag gcgcccgcct ccacgcccag ctaatttttg tatttttagt agagatgggg 4021 tttcgccatg ttggccaggc tggtctcgta ctcctgacct tgagtgatcc acctgcctcg 4081 gcctcccaaa gtgctgggat tacaggcatg agccaccgcg ccgggctaat atgctcattt 4141 tagtgaggca aaaatagagg ctcagagtct gatttgtaca aaactacaga gcagttaagt 4201 gtcctctcag atgtgtaccc tgatctgggt gactctagga ctctaggtct caactgttac 4261 aaccagttaa gggtttgggg aagcactggg ccaagagtca ggaaaatgga agccacaggt 4321 agtgcaaggt cttgggaatg ggacgtctgg tccaaggatt cacgcgatga ctggaacccg 4381 aagagccggg gcccggttta cggccgccat gaagcaacgc gcgccggtag gtttgggaat 4441 cagggagccc tctgaatagg agactgagtt gggagggaaa ggggcttcgc tgggggagcc 4501 tcggcttctt ctgggagaaa attcccacgg ctacctagtg agcctgcaaa ctggtaggcg 4561 ccggcgtagg cgcgcgggcg gggccggggg cggggcctgc ggggcgtggc ggggcgggca 4621 gagggcgggg cctgcttctc ctcagcttca ggcggctgcg acgagccctc aggcgaacct 4681 ctcggctttc ccgcgcggcg ccgcctcttg ctgcgcctcc gcctcctcct ctgctccgcc 4741 accggcttcc tcctcctgag cagtcagccc gcgcgccggc cggctccgtt atggcgaccc 4801 gcagccctgg cgtcgtggtg agcagctcgg cctgccggcc ctggccggtt caggcccacg 4861 cggcaggtgg cggccgggcc ctgaggcgcg ggatcgatcc atgacacccc cccgcccccc 4921 atatctcact ataattcact gtcaaatggc ttttatgatt tggagtttgg ggatactttt 4981 aaagacaggg tcttggtagc tcctagatgg cttgggattc acttagtaac ccttgctggc 5041 ctaaaattca ggaacttcta cctcatcttc ccaagtactg ggttagtagg gatgtccagc 5101 atatttagct tgctttaatg attttgctgt acttttttct gtgtgtacag gtgcatgtat 5161 atttataaaa ctaacataat atattttacc cccaaagtac cataacccta attaatgcta 5221 ttaaatgttt tgtatgctgg gctctgggca tactaatata accaatatta tacaatgcaa 5281 aacaattttg gaatatcttc tgtaaaatta tcttattagt aagttgtaac catctcagac 5341 ttctaagttt gagtttgtaa aggatcagtt ttttttggtg acctacacac acacacacac 5401 acacacacac acacacacac acaggggggt ggattctgag aatttagaga atttagatgg 5461 ttaataccag tttttatttt tattctgtgt gatatttatt tattaagatt tatttattta 5521 ttacatgtag gtacactgta tctgtcttca gacacaccag aagagggcat cagatctcgt 5581 tacagatggt tgtgagtcac catgtggttg ctgggatttg aactcaggac ctctggaaga 5641 acagtcagtg ctcttaaccg ttgagccatc tcaccagccc cgtgatattt tatttaactt 5701 actagaatat actgtgttaa aacatatttt aggggattgg ggagatggcc cattctgtaa 5761 agtgtctgcc atgtcatctt caggacctgc gtttgtaacc ccagcatgga gaagacagag 5821 acagggagac ccctgggctt cctggcaagc tagactagca aatttggtta actaggttca 5881 atgagagact cagtctcaaa acataagaag tgaatttttt agacccaatt tcaatctctg 5941 aactccatac gtatgcacat gtacctgcat ataaatatat atacacaccc ataaacacat 6001 gcacacacaa aaaatggttc aaaagttctt aaaatgtata agagtagaaa gtgtagagcc 6061 gggagtggtg gcgcacgcct ttaatcccag cacttgggag cagaggcagg tggatttctg 6121 agttcgaggc cagcctggtc tacagagtga gttccaggac agccagagct atacagagaa 6181 atcctgcctc aaaaaaccaa aaataaagtg tatatactaa tctttcatgc ctaaaatatt 6241 tatttttgcc acatttttca tacctcttta atatttttct ttttctcttt gtgcttcaaa 6301 ggaaattttg tgtcatgttt tacttttcat tcttgactct attcagcagt aagacgcagc 6361 atggctttct tagagaagta taatctatta caaaacggta acatttaact gctctacata 6421 ccctcttgta gctaaattcc tctgttaaac taagacttat tatctaaacg taattaaatg 6481 ttgatatctt ataatgctcc actttgaaac agctgtcttt cttttgcaga ttagcgatga 6541 tgaaccaggt tatgacctag atttgttttg tatacctaat cattatgccg aggatttgga 6601 aaaagtgttt attcctcatg gactgattat ggacaggtta gtaagacctc gattgaagtt 6661 atttttagag ggtgtgtgtg tggtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 6721 gtaagagaga gaggagaggc atgtgtgata tatatgtgaa gtgtattgca tgtacatgga 6781 atccagagaa ggacattagg tgtcctactc tgttgctctc ttattccctt gaatcagtgt 6841 ctcttgctga acctggggca tacatactga tttggctagg ctgcctggct agcaagaccc 6901 agggatctgc ttgtctctgc cccccagtgc tgacgttaca agcaggtgca accacatctg 6961 gtttttatat gggtactggg ggatctgaac ttagggcctg atgcgtgccc aagtgctctc 7021 acccgccaag ccacctctcc agccaaaggg ttttttttct tgtattaatt ctaacttagc 7081 aactgttgag tagttgatgt ttgaatataa gctaagccaa cttattagaa taaatgtttt 7141 ctatttttta cttgtatgtg tgagtgcctg catgtatgta tgtgaagcat atgtattcca 7201 ggatgtcaga attgtgatgg attccctgga actggagtta cagaccattg tgaaccactg 7261 tgtatgggtg ctgggaactg aactttaggt cctctgcaaa aacagtaagg atcttaactg 7321 ataaactatc tttctagccc taaggctttc tacttttaaa gttcgtttct ctttctctct 7381 ctctctctct ctctctctct ctctctccct ctccctccct cctcctcctc ctcctctttc 7441 ccccctctcg cctcctcttt ctctctcccc tccctctctc tctctcacac acacacacag 7501 acagagagag gagagagaaa ggaagcctta ctaattaatt acctagtttt agacataact 7561 agtgggaatc catagtaagt aaatcaacat tcttgaagag aggttagaag aattggctct 7621 cgttgtccat cgaggctgta taagattatt cttctgtttt ctcagcttac catacatatt 7681 ttctacgtag acaataccaa ttcaaataca ctgtacgtaa agtatatgcg cacacacaca 7741 cacacacaca cacacacaca cacacaagtt acatattgtc atatagtact aaaatttatg 7801 gactttttca tattaataca tctagagcta tcatgttttt tatcttttaa aaattacttt 7861 acggggctac agagatgtct cagcagttag gagcactgac tgctcttcca gaggtcctga 7921 gttcaattcc cagcaaccac atggtggctc acaaccatct gtaatgggat ctgatccact 7981 cttatggtgt gtctgaagac agtgacagtg tactcatata catgaaataa atctttaaaa 8041 aattacttta ccatcagggt atatatatat atatatatgt gtgtgtgtgt gtgtgtgtgt 8101 atgaagtatt tgctatttga gagggtctca ctgtttagct cagtctggct tggaacgttc 8161 tgtatagccc agactggcct caactcagca gtccccctga aaggctctca tatgtggaga 8221 ttacaggtat gtgctactgt gcccagccct acatgttact ttgtttttga aatagggtat 8281 caaatagctt agactggctt ctaagtcagt gtgtagctaa ggatggtctg agcttctgaa 8341 cctgctactt caactcctgg tgtgccatca tggccagttt gcacagttgt gagagtagaa 8401 ctcaggacct cctatgtgtt actagacatg ttctctacca gctgagctac atcccaattc 8461 gcctacattt acttttattt tttaaaagat ttatttatgt atacagcact ctgcctgcat 8521 gtacatctgc atgccagaag agggcaccag atctcatcat acatagttgt gagccaccat 8581 gtggttgctg gggattgaac tcagggcctc tggaagagca gtcagtgctc ttaacctctg 8641 agccatctct gtagccccct acatttattt taaagattat aggctattct attcatagat 8701 gtgccataac tgatttaacc agatcttcat tattttgtac ttagtagagt atattagagg 8761 gaatgaatct taaatacaca ttttgacact tgctcagctt tagacaagta aaaatcactg 8821 gtcaaggaaa tggtgcttag tggatgcttc tgaactgtca aattgtcctc caacaagtta 8881 catcatttta tacttccaac gttatgtgag agttcatttt tctttaggct cttctagtat 8941 tttatatttg agaaataaaa tttaaccaaa atttgccaat ctagtaagct tgtttttatt 9001 tgtatcttta aattttatga tagatgttat agtgtactct cctctcccct cccctcccct 9061 ctcctcctca gcaggatctt gttatgtagc cctaagggat ctaatactta ctctgtagtc 9121 caggctgtcc ttcagctcat gagacacacc atcatgcttg gcatgtttct tgttctgaaa 9181 ggtatctggt tttaattttg gttgagctgg tgtgttggtg tatatctgta atctcagcac 9241 aggcaagtag agacaaggat tatgattaca gtccagcctg ggctatttag agggattctc 9301 tttaatcttt cctcatgccc caaaatctta cctttggtat atgaaaaata gtctccactt 9361 ctgcaaaata ttgctttatg aagtaagaat taattccctt catagagaca aggaatgtgt 9421 cctgtaaaag tttaatgtgt aagaagtatt tgttataaaa gataaatatt cagaatcttc 9481 tttttaattc ctgattttat ttctatagga ctgaaagact tgctcgagat gtcatgaagg 9541 agatgggagg ccatcacatt gtggccctct gtgtgctcaa ggggggctat aagttctttg 9601 ctgacctgct ggattacatt aaagcactga atagaaatag tgatagatcc attcctatga 9661 ctgtagattt tatcagactg aagagctact gtgtaagtat aattaactta taattaaaaa 9721 aatagggcca ttctagtttt atctatattt tttttaaact tgtgcaaact atgctacata 9781 atcaacttac ttgattatgt aatatatata gtaatagttg gaattactat tattacatat 9841 ttatttttct gtgctggggt agaacccagg gaactaagta ctgtaataac cgagcttcat 9901 tcatcctagt catttttaat tttttttttt ttttttgttt tttcgagaca aggtttctct 9961 gtgtagccct ggctgtcctg gaactcactc tgtagaccag gctggcctcg aactcagaaa 10021 tctgcctgcc tctgcctccc agtgctgaga ttaatgacgt gcaccaccac tgcctggcat 10081 ttttttaaag ttttaatttg agaccctttc tcactaactt gctcacacta tcctcgaact 10141 cattacgtgg gctggtctag aaatttcagt caacttgcct cagcttctca agtagctggg 10201 attacaggca ggtgctacca cttctggcca tgatcattgt tgttgttgct attattatta 10261 ttattatttc actgatactt catatcacaa cacagaatag taactgtagt tcatgaccaa 10321 caaaaaagtt gtacagtttc tctgttgtct tattttgatc tggattttga tttgtttcat 10381 ggcactgggc aggctaatct caaactgcat ggctcaaata gtccttctgt cttaatctcc 10441 catgttctgg aagtataggt atcactacac ctagctgtat tttcatattt taaaaaaaac 10501 gcctaagaat tgctattgaa tgaggcggag agatggctca gcagtcacaa gtacttgtag 10561 aggactgggg tttggttact agcacctcaa agatggctta caattatctg tagctccaat 10621 tccagaggat ctgtgtggta aagctgcatg tatatgggca tgcaggtaaa actgctaaac 10681 atataaaaat agctcagtct taagagaatt ggtcgactgt cgactttttc gccctttgac 10741 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 10801 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 10861 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat 10921 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 10981 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 11041 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 11101 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 11161 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 11221 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 11281 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 11341 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 11401 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 11461 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 11521 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 11581 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 11641 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 11701 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 11761 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 11821 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 11881 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 11941 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 12001 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 12061 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 12121 caaacaaaaa aaccaccgct accaggtcga gtagggataa cagggtaatg gatcctcgtt 12181 gggcacctgc actcatgtac gcaggcccac atatagatgt acacacgcat atcattcaaa 12241 atagaaatta tttatacaag tagtgcgttg gagagctggc tcaggagttg ttaatgcttg 12301 ctacccttgc aaaggaccca agttcaattt ccagcacctg tgttgggcat ctcacaacca 12361 catgtaaatc tagtttcagg ggatttcacc cctcttctga actcttcagg cacttatacc 12421 cacaaatgga catctccaca tagagacaca tacttttaaa aaagtaatct agggtctaga 12481 gaaatggtta tagaccacgg actgggatca gttcccagaa gccacatcct gtggctcaca 12541 attgcctgtg aatcaagttc tagatctggt ggcctcgtct agcctgcagt catcaacaaa 12601 cacatgcaca catccataca cacataattt aaaagaaagc gaaagtaacc tataatgcta 12661 aacaaagcac attctcagtg tagactgcag tggtgatgaa ccttgaaaat atattaatag 12721 aagccagaca cgtaagtcac aaatggtata actcaattta tatgaaattc ccagagagtc 12781 caaagttaaa aaaaacaaaa aggcagatta ttggttgtca gtagctacag agatgggaca 12841 ttgaagatat taataggtat agggttgagg attcatggcc tttccctctt ccatgttagc 12901 atgtctattg ttgttaacct tattcaactc atgttagggc aatcatattg atgagactta 12961 atgagtatag cttgacattc ctagaagaca gagtctcaca tcagactctc tgatcctctg 13021 actcttagaa tctttcaaca ccctcttctg aaatgatctc tgagcctttg gttgtgactg 13081 aattatgaga aagaatttta tgttgaatga attttacttc agcattttta caaaactgaa 13141 gctggggatg gcgtttcatg cgtgcagtcc ctaccatttg cctataagcc tggaggaggc 13201 agaagtagaa ttaggctttt cagggacatc ttcagctgca tcatgagctt gaggacaact 13261 tgagctacat aagattccca tccccacccc cacacacaca cacacatcta gtatgtacct 13321 agtatgcagt cttttcatat gtttaaaatg tagaaaggta tatgtgaagt ctcccttttc 13381 cccacaactt agtccctcca aagtattgca tcagtttcct tgtacatgat tttatgcata 13441 tataagatac actatttctc tctctctctc tctccttccc tccttccctc caacctctgg 13501 gtatgagttg agggttatgt atgtttcaag ttgtaataag aattgccact caaacatttc 13561 atcccatttt aagttaagtt tgtctgattt tccactttag aagggcagcc atgatggctc 13621 atgcctataa tcccatccta gcacttgtaa gcctaataca taagactgga ggattgtcat 13681 cagcttgagg ctagcctggt ctacctaatg agttccagcc agccaagagt agaatataag 13741 accctgtctc caaaaaatgt accaaagggg ctggaggggt aactcagtgg ttaagaacag 13801 tagctgctct tccagaggac ctgggttcaa ttcccagcac ccacatggca gcttgcaact 13861 atctgtaact gtccagcttt gggatatccg acaccattat accaattaca taaaatgaaa 13921 aaattgtaaa atgtgctaaa atgagtgggg agggggagca tggtgtggtt ctgtgggctt 13981 gggattttgc catgaatcca tgactatatc tcagcaatat gtggattatt tctggaaacc 14041 tgtggggcat gggtgtatgg gaagagggga gagagagaga gcccgcatcc agccagagtt 14101 cctgtgctct gggcaggcgg acgtgggagg actgctggac gatttccact tggccctggg 14161 tgggcatctg gctgtgtgaa gccactgacc ccactcagcg gggggtagat aaggggcatt 14221 tcccatacca ggttctcaga ctccagcatt ccacgctgga tacagcagaa gagctgagaa 14281 cagaataggg gccccaggga gacactctcg tcccagagat aaagaaggca gagggagaga 14341 gattcctacg caggcgagag gccttagtct gggccttgat gaacagagac ggtctatggt 14401 tttagagctt tattgtagaa aggcaggggg aaagagagaa ggtagaaaga gagagaccag 14461 ccatggccaa gaggagagaa ggggggaaag gagagagaga agaaaggcta gagagtaaga 14521 aaggttaaag agacagaagt aaaagcttag agtaagagag agagagagag caagagggag 14581 agtaagaaaa gtaaaagctt aagagagcga ggaggggcca gcccctctaa tagtgggctt 14641 gttatcttgt tgttgctagg taactgtgaa gcgtctagcc tgaaggccag aagcttggga 14701 cattgtctat gtgactgaaa gccatgagcc tctactgtgg gggctgtgga ggtggtaact 14761 tggacaggag ccaggggttc caggagacat gagggaatgc cttctgtccc atgtaggtag 14821 gaattaccac caccggcttt caccgtagtt cactggagac tgtttgtaca tagcccattg 14881 ccccacagaa accaggcact cactgtttca tttgcataag aattagaagg ctttattttg 14941 gctgggattg gtgacttgcc ccagaaattc cagcttttgg gaagccgaat cctggagaac 15001 caggagttca aggtcatcct caggtacctc atttgttcaa gaccagcctg tgctaccatg 15061 agaccttgtc cctactcccc tcaaccccaa aacacgctgt tttcatttta taggttgtac 15121 taaccatgga ggatggtcat ttgcctttct cttgatttca gaagcgtagc gcgggctttg 15181 caccatgatc tagtgtttcc agataggctt gcctctccac agccaggctt atcctgcttc 15241 agtcccatgt ttggcaaggg caacatatga ctgaagaact tgcatagcct tatggtgtat 15301 catgactctt agccccagtt tataactgtc taacagctgg tggcttttgt gaaaaaggaa 15361 agcatcattt gaacttttaa cttttcagct gtttgttttg actttgcata aatactgaga 15421 ctagctagga aatgagcttt tgtttcattt gctaaaaatg attttttttt catgaggcaa 15481 tttcaagcaa tttgaattta gcaatctcta cttgtctgaa aagaagttat taattaaaaa 15541 aaaacaaaca aacaggaacc tattatgctg gctagtcaca tgaaagaatc caaatgaaca 15601 cagtcaaggc tgaatcaaca catgaaatta ttttctaatt ccccaaatta catttatgct 15661 aattagtatg tagtaaaaca ttttaattgt ttccaatgtt aaacattaca gaaataattt 15721 tgatgttttc ctaataaccc agcctttgat catgtttgca tttatttgat tgttgtcagt 15781 ttattaaata attaagtaag agcaacaaga taaaatgact tcatgagagt ccttttctgt 15841 aacttagaac gtcagtagtc ataggaactg cggtcgttcc cttacctttc agaagtaggc 15901 taaagagttg aacgcaaagg tggtttttcg aatcttcctg atccttaagc ccccactagg 15961 tttatattct atatttgata tcggattcga acccctcgag cttaggatcc ggaaccctta 16021 atataactaa gttattaggt ccctcgaaga ggttcactag tactggccat tgcggccgct 16081 tcgaggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 16141 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 16201 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 16261 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 16321 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 16381 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 16441 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 16501 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 16561 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 16621 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 16681 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 16741 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 16801 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 16861 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 16921 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 16981 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 17041 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 17101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 17161 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 17221 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 17281 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 17341 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 17401 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 17461 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 17521 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 17581 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg 17641 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 17701 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 17761 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 17821 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 17881 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 17941 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 18001 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 18061 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 18121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 18181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 18241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 18301 cctgacgg //