LOCUS Ple176\(pEMS1088 20676 bp DNA circular 23-JAN-2009 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2406 5'- CATGAGCAAAAGAAGATGGGACAGTm= 56.7 Deg C Antisense primer: oEMS2367 5'- GTGGAAGGAAAAGCGGCAAG-3' Tm=56.2 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 548 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|507991750| COMMENT VNTDBDATE|507991750| COMMENT LSOWNER| COMMENT VNTNAME|Ple176 (pEMS1088)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#205=(AnlzFontBackColorItem 2507 2530 43633766))) (CObList) 1699618931 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers misc_feature 3378..3411 /vntifkey="21" /label=MFRT\(F5\mutant) misc_feature 3412..3423 /vntifkey="21" /label=Kozak\consensus\sequence misc_feature 3424..4140 /vntifkey="21" /label=EGFP misc_feature 4141..5166 /vntifkey="21" /label=Cre misc_feature 5167..5239 /vntifkey="21" /label=NLS misc_marker 5243..5276 /vntifkey="22" /label=MFRT misc_feature 5430..5480 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 5486..9439 /vntifkey="21" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, mouse Intron1, mouse Exon2, and part of mouse Intron2" misc_feature 9440..13085 /vntifkey="21" /label=3'\homology\sequence /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_feature 14539..18350 /vntifkey="21" /label=5'\homology\sequence misc_marker 13087..14513 /vntifkey="22" /label=AmpR misc_marker complement(19670..20670) /vntifkey="22" /label=AmpR misc_feature 14520..14537 /vntifkey="21" /label=I-Sce\I misc_feature 139..3369 /vntifkey="21" /label=Ple176\(RGS16-B) /note="1302" misc_feature 139..1743 /vntifkey="21" /label=Region\1 misc_feature 1744..2788 /vntifkey="21" /label=Region\2 misc_feature 2789..3369 /vntifkey="21" /label=Prom\region primer 3036..3055 /vntifkey="27" /label=oEMS2367 primer 2508..2531 /vntifkey="27" /label=oEMS2406 BASE COUNT 5405 a 4742 c 4862 g 5667 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccgg aagtgtgcac accagttgtt agtgactatc attgtgttta 181 ctgcagccag ggtgtttggg acgcctgggg acttggatcc agaaagttcc cctgtggtca 241 catttggagc agcactacag ctagatggct ggcggaggag gtgtggctca cccaggctca 301 gtcacacagc tcaccgcgtg tgcccccctg ctcaaatgtg tggggcctat tagggagagc 361 tcatttctat ctctgcaaaa acatcatgca ccccctgctg tgctcttctc tctgtccacc 421 tgggcctgtt tctaggacag ctgctgtgtc aggggctgag ggcctgggag cttgtgaaag 481 gaccacctgt tcccattgct ttctccggcc ctcctccccc gccgccactc tgccggccca 541 gcctcccagc tgctcacaca tcacaccacg tttctccctc tgctcaggtg ttgtgagcac 601 attgtgagag gcatgatccc ttccaacttg tcgccacatt acagctagtg ttggtgcttt 661 tgttgagcct cccctgcttt cctataaaat ggtttttctt ctgtgcccct gcaccagact 721 gccccacccc caccccctac tctccctgtt gacatgggga ctcctggaag gtgactcaaa 781 ggaggaggag gtaccccaca gaaagtaggt ccttaactcc caggagatga caaagcccct 841 cccatgcctc tgacctgaca tctctgcacg tgtgtgtggt attcacatgt tggggcttgc 901 tgttcctggg gtccttatgt ctcatctgtg gcctaggtaa gcacctagaa atttctgact 961 agaacaagga ggagaaatgg cctgaagtag cagccaaggt ccagggggag agatgctcag 1021 aggctaggga acatggggac aaggcattga atagccctgg ggttggacct tgtggataga 1081 actgggtttc tagcctggca ctcccattca ctaggtgtgt cactgtgggg agattactta 1141 cgcaccgatg tctgtttccc cttctgtaca ggtggcatct gtaaggccct gagtccagcg 1201 ttggatcatg gagggcccta aaaatggtag tttccttttc tctaagacag ggcctggagg 1261 aagaccagac atcaacctcc caagctggct gaaatcgtct tactgtctga cacagagccg 1321 ggttctccct agtacatgct gctctggaat acctaggaga ggaagaggaa ggaggcaagg 1381 taagctgggg gcttttcaca ctaaaggcct acagaggggt aggtggagtc ggcataggtg 1441 ccatgtgtgt ttggtgtctt gcttccgact ctagctgggc tctggagcca ctgatgtctg 1501 cttgaccctt ccctgatttt gctccagttt gctctgcact gggagactgc aagccacagg 1561 gggaggggag tgtaagaggg ctgcagagta ataagtttca ctgcataatt cagccctcct 1621 gggacccgtt tccagaaagc tgactggctg aagtttagct gagaatgcct gcaatctgga 1681 ggatctgggg caggcagggt tgacagttaa tctccaagga tattcaccat gtttggagtg 1741 ggaaaaggac taggggacgg gaaatataaa catcaccttt cagtcatttg caaatcccag 1801 gcctcacgtt tcccccctct aatctttcat tctgactggc ttctcagggt cttggaaaat 1861 cattacagta aaatgcagat tctaataagc cttgtttcta agtcttagtg aatggaaatg 1921 agatactctc agaaaaatga cttttgtgac aaaggtgaag aaggcaagga ggaggtatgg 1981 tgggaaagag gaagacattt agaaactcag gcttcttagg taactctctt acttcggaaa 2041 tggaagtgtt aaggtgagat gggcccattt attactgatg acttacttgg tactttaggg 2101 aatgcagcaa ggtgatggca ggggtgggca ggggtttgca gagtggagac agcatgggct 2161 ggtgagcaca gggagaggtg gaggaagctg ggcgctggca ggcagggaaa gttcgggagc 2221 ctggagggag gtagactatc gggttatagg gtggggtgaa gtggacctta cagtcaggca 2281 ggtaagctca cgggtttcag accacaaaac gctaagatct gggcctggga agggccaagc 2341 cagcatgtga gaaccaagaa agcataaacc tcagagggcc aggcgccatc ccccagctct 2401 caggttccca ggaggatggg gaggaacaaa ggtcttgggt tttgacacat cccaaagact 2461 ttgggagctg cccctggtct ccctcccagg tgggaaccag gcttttccat gagcaaaaga 2521 agatgggaca gcatgggctg gaaagtgtgg caggggaaca cagagctgaa ttctgagttt 2581 tcctgaaaaa gagatataaa aacccacatg ctaacagagg gacttgagca gagaaaggga 2641 ctctccacag ccatactcgg gaactgtata cacaagggac tgggaagtga agcagcagaa 2701 aaatccagag cctgtgatga catcacagct aagggcaaag acaggaagtg gaaaatgtga 2761 atggggaggg aggaatggat tgagcaaggg gtacttatca gtataatcag atgctgtttt 2821 gcccaggttt gcaccctgtg cgcctgtcct caggacccag caggctcaga cttgcctgcg 2881 atcaaagaaa gccctgtgtg cctcccagac ccaccacccc cgtgcaaact ctctcaacac 2941 gacaccgctg cgtgactttc ctgctgttac tagaggtcgg caggtacttt tccctaggaa 3001 actgggtgtg cacgtctcat ccccgtgcca attctcttgc cgcttttcct tccacacgcg 3061 gcgtcctctg ggcggagggt gcagctccgc gtgctccagc tgccgcgaaa gttgcacccg 3121 gcccagtctg gctgcgggcc ccgcctccgc cttctcagcg cccagcccca ccctccgatt 3181 ggtcagcagt gcccagcccc gccccgctcc tccgagggga tttaaagcag cggccgctca 3241 gtctgggcgc ttgcaggctg ctaaacccaa ccgcagttga ctagcacctg ctaccgcgcc 3301 tttgcttcct ggcgcacgcg gagcctcctg gagcctgcca ccatcctgcc tactacgtgc 3361 tgccctgcgg gcgcgccgaa gttcctattc ttcaaaaggt ataggaactt cgccgccacc 3421 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 3481 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 3541 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 3601 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 3661 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 3721 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 3781 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 3841 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 3901 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 3961 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 4021 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 4081 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtta 4141 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat 4201 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc 4261 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg 4321 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc 4381 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg 4441 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga 4501 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat 4561 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg 4621 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg 4681 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa 4741 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc 4801 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg 4861 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg 4921 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag 4981 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc 5041 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc 5101 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat 5161 ggcgatgatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtagatcca 5221 aaaaagaaga gaaaggtaat aagaagttcc tattctctag aaagtatagg aacttcagcg 5281 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 5341 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 5401 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 5461 taaagcattt ttttcactgc gaattctcgt aaaactcttc atggcagtag ttattattct 5521 ctctctctct ctttttcttt tttcttgaga caggatattt ctctgttgcc caggctggag 5581 tgcagtggca cagtcttggc tcactgcagc ctggacctcc tgggctcaag ccatcctccc 5641 acctcagcct cccaagtagc tggggctaca ggcacatggc caccaggccc agataatttt 5701 tcatttttgt agagactgag tctcaccatg ttacccaggt ttattattct cattttttag 5761 atgaagagac tgaggtccag agaagctcaa tgacttgcct agttttacaa atctcctgcc 5821 atcacatacc cctcagcgtc cttaataaga gggaggccac caactatgtg ctgggcactg 5881 tggtggatgc tggagctata gggttgagta tataagaaat ggtgttgctg gagcaactgt 5941 tgcttgctta cctgacctat ctgagaatta attagcaggg gaacatattt ttgttttcag 6001 attcaatata agaacttgtg tgggcaaaaa taaagatcag tagtaataac agtagttccc 6061 atttgctgac tgtactgtcc taagtgcata tatatataca tacacacacg catacctata 6121 ctcctctaat actcaaaatg atcctgttta tgtattgtta atatgctcat tttattttta 6181 aatttttatt tatttttatt tttatttatt tttgagacgg agtctcattc tgtcgcggag 6241 gctgaagtgc agtggtgcga tctcagctca gtgcgacctc cgcctcccgg gttcaagtga 6301 ttctcctgcc tcagcctccg gactagctgg gattacaggc gcccgcctcc acgcccagct 6361 aatttttgta tttttagtag agatggggtt tcgccatgtt ggccaggctg gtctcgtact 6421 cctgaccttg agtgatccac ctgcctcggc ctcccaaagt gctgggatta caggcatgag 6481 ccaccgcgcc gggctaatat gctcatttta gtgaggcaaa aatagaggct cagagtctga 6541 tttgtacaaa actacagagc agttaagtgt cctctcagat gtgtaccctg atctgggtga 6601 ctctaggact ctaggtctca actgttacaa ccagttaagg gtttggggaa gcactgggcc 6661 aagagtcagg aaaatggaag ccacaggtag tgcaaggtct tgggaatggg acgtctggtc 6721 caaggattca cgcgatgact ggaacccgaa gagccggggc ccggtttacg gccgccatga 6781 agcaacgcgc gccggtaggt ttgggaatca gggagccctc tgaataggag actgagttgg 6841 gagggaaagg ggcttcgctg ggggagcctc ggcttcttct gggagaaaat tcccacggct 6901 acctagtgag cctgcaaact ggtaggcgcc ggcgtaggcg cgcgggcggg gccgggggcg 6961 gggcctgcgg ggcgtggcgg ggcgggcaga gggcggggcc tgcttctcct cagcttcagg 7021 cggctgcgac gagccctcag gcgaacctct cggctttccc gcgcggcgcc gcctcttgct 7081 gcgcctccgc ctcctcctct gctccgccac cggcttcctc ctcctgagca gtcagcccgc 7141 gcgccggccg gctccgttat ggcgacccgc agccctggcg tcgtggtgag cagctcggcc 7201 tgccggccct ggccggttca ggcccacgcg gcaggtggcg gccgggccct gaggcgcggg 7261 atcgatccat gacacccccc cgccccccat atctcactat aattcactgt caaatggctt 7321 ttatgatttg gagtttgggg atacttttaa agacagggtc ttggtagctc ctagatggct 7381 tgggattcac ttagtaaccc ttgctggcct aaaattcagg aacttctacc tcatcttccc 7441 aagtactggg ttagtaggga tgtccagcat atttagcttg ctttaatgat tttgctgtac 7501 ttttttctgt gtgtacaggt gcatgtatat ttataaaact aacataatat attttacccc 7561 caaagtacca taaccctaat taatgctatt aaatgttttg tatgctgggc tctgggcata 7621 ctaatataac caatattata caatgcaaaa caattttgga atatcttctg taaaattatc 7681 ttattagtaa gttgtaacca tctcagactt ctaagtttga gtttgtaaag gatcagtttt 7741 ttttggtgac ctacacacac acacacacac acacacacac acacacacac aggggggtgg 7801 attctgagaa tttagagaat ttagatggtt aataccagtt tttattttta ttctgtgtga 7861 tatttattta ttaagattta tttatttatt acatgtaggt acactgtatc tgtcttcaga 7921 cacaccagaa gagggcatca gatctcgtta cagatggttg tgagtcacca tgtggttgct 7981 gggatttgaa ctcaggacct ctggaagaac agtcagtgct cttaaccgtt gagccatctc 8041 accagccccg tgatatttta tttaacttac tagaatatac tgtgttaaaa catattttag 8101 gggattgggg agatggccca ttctgtaaag tgtctgccat gtcatcttca ggacctgcgt 8161 ttgtaacccc agcatggaga agacagagac agggagaccc ctgggcttcc tggcaagcta 8221 gactagcaaa tttggttaac taggttcaat gagagactca gtctcaaaac ataagaagtg 8281 aattttttag acccaatttc aatctctgaa ctccatacgt atgcacatgt acctgcatat 8341 aaatatatat acacacccat aaacacatgc acacacaaaa aatggttcaa aagttcttaa 8401 aatgtataag agtagaaagt gtagagccgg gagtggtggc gcacgccttt aatcccagca 8461 cttgggagca gaggcaggtg gatttctgag ttcgaggcca gcctggtcta cagagtgagt 8521 tccaggacag ccagagctat acagagaaat cctgcctcaa aaaaccaaaa ataaagtgta 8581 tatactaatc tttcatgcct aaaatattta tttttgccac atttttcata cctctttaat 8641 atttttcttt ttctctttgt gcttcaaagg aaattttgtg tcatgtttta cttttcattc 8701 ttgactctat tcagcagtaa gacgcagcat ggctttctta gagaagtata atctattaca 8761 aaacggtaac atttaactgc tctacatacc ctcttgtagc taaattcctc tgttaaacta 8821 agacttatta tctaaacgta attaaatgtt gatatcttat aatgctccac tttgaaacag 8881 ctgtctttct tttgcagatt agcgatgatg aaccaggtta tgacctagat ttgttttgta 8941 tacctaatca ttatgccgag gatttggaaa aagtgtttat tcctcatgga ctgattatgg 9001 acaggttagt aagacctcga ttgaagttat ttttagaggg tgtgtgtgtg gtgtgtgtgt 9061 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt aagagagaga ggagaggcat gtgtgatata 9121 tatgtgaagt gtattgcatg tacatggaat ccagagaagg acattaggtg tcctactctg 9181 ttgctctctt attcccttga atcagtgtct cttgctgaac ctggggcata catactgatt 9241 tggctaggct gcctggctag caagacccag ggatctgctt gtctctgccc cccagtgctg 9301 acgttacaag caggtgcaac cacatctggt ttttatatgg gtactggggg atctgaactt 9361 agggcctgat gcgtgcccaa gtgctctcac ccgccaagcc acctctccag ccaaagggtt 9421 ttttttcttg tattaattct aacttagcaa ctgttgagta gttgatgttt gaatataagc 9481 taagccaact tattagaata aatgttttct attttttact tgtatgtgtg agtgcctgca 9541 tgtatgtatg tgaagcatat gtattccagg atgtcagaat tgtgatggat tccctggaac 9601 tggagttaca gaccattgtg aaccactgtg tatgggtgct gggaactgaa ctttaggtcc 9661 tctgcaaaaa cagtaaggat cttaactgat aaactatctt tctagcccta aggctttcta 9721 cttttaaagt tcgtttctct ttctctctct ctctctctct ctctctctct ctctccctct 9781 ccctccctcc tcctcctcct cctctttccc ccctctcgcc tcctctttct ctctcccctc 9841 cctctctctc tctcacacac acacacagac agagagagga gagagaaagg aagccttact 9901 aattaattac ctagttttag acataactag tgggaatcca tagtaagtaa atcaacattc 9961 ttgaagagag gttagaagaa ttggctctcg ttgtccatcg aggctgtata agattattct 10021 tctgttttct cagcttacca tacatatttt ctacgtagac aataccaatt caaatacact 10081 gtacgtaaag tatatgcgca cacacacaca cacacacaca cacacacaca cacaagttac 10141 atattgtcat atagtactaa aatttatgga ctttttcata ttaatacatc tagagctatc 10201 atgtttttta tcttttaaaa attactttac ggggctacag agatgtctca gcagttagga 10261 gcactgactg ctcttccaga ggtcctgagt tcaattccca gcaaccacat ggtggctcac 10321 aaccatctgt aatgggatct gatccactct tatggtgtgt ctgaagacag tgacagtgta 10381 ctcatataca tgaaataaat ctttaaaaaa ttactttacc atcagggtat atatatatat 10441 atatatgtgt gtgtgtgtgt gtgtgtgtat gaagtatttg ctatttgaga gggtctcact 10501 gtttagctca gtctggcttg gaacgttctg tatagcccag actggcctca actcagcagt 10561 ccccctgaaa ggctctcata tgtggagatt acaggtatgt gctactgtgc ccagccctac 10621 atgttacttt gtttttgaaa tagggtatca aatagcttag actggcttct aagtcagtgt 10681 gtagctaagg atggtctgag cttctgaacc tgctacttca actcctggtg tgccatcatg 10741 gccagtttgc acagttgtga gagtagaact caggacctcc tatgtgttac tagacatgtt 10801 ctctaccagc tgagctacat cccaattcgc ctacatttac ttttattttt taaaagattt 10861 atttatgtat acagcactct gcctgcatgt acatctgcat gccagaagag ggcaccagat 10921 ctcatcatac atagttgtga gccaccatgt ggttgctggg gattgaactc agggcctctg 10981 gaagagcagt cagtgctctt aacctctgag ccatctctgt agccccctac atttatttta 11041 aagattatag gctattctat tcatagatgt gccataactg atttaaccag atcttcatta 11101 ttttgtactt agtagagtat attagaggga atgaatctta aatacacatt ttgacacttg 11161 ctcagcttta gacaagtaaa aatcactggt caaggaaatg gtgcttagtg gatgcttctg 11221 aactgtcaaa ttgtcctcca acaagttaca tcattttata cttccaacgt tatgtgagag 11281 ttcatttttc tttaggctct tctagtattt tatatttgag aaataaaatt taaccaaaat 11341 ttgccaatct agtaagcttg tttttatttg tatctttaaa ttttatgata gatgttatag 11401 tgtactctcc tctcccctcc cctcccctct cctcctcagc aggatcttgt tatgtagccc 11461 taagggatct aatacttact ctgtagtcca ggctgtcctt cagctcatga gacacaccat 11521 catgcttggc atgtttcttg ttctgaaagg tatctggttt taattttggt tgagctggtg 11581 tgttggtgta tatctgtaat ctcagcacag gcaagtagag acaaggatta tgattacagt 11641 ccagcctggg ctatttagag ggattctctt taatctttcc tcatgcccca aaatcttacc 11701 tttggtatat gaaaaatagt ctccacttct gcaaaatatt gctttatgaa gtaagaatta 11761 attcccttca tagagacaag gaatgtgtcc tgtaaaagtt taatgtgtaa gaagtatttg 11821 ttataaaaga taaatattca gaatcttctt tttaattcct gattttattt ctataggact 11881 gaaagacttg ctcgagatgt catgaaggag atgggaggcc atcacattgt ggccctctgt 11941 gtgctcaagg ggggctataa gttctttgct gacctgctgg attacattaa agcactgaat 12001 agaaatagtg atagatccat tcctatgact gtagatttta tcagactgaa gagctactgt 12061 gtaagtataa ttaacttata attaaaaaaa tagggccatt ctagttttat ctatattttt 12121 tttaaacttg tgcaaactat gctacataat caacttactt gattatgtaa tatatatagt 12181 aatagttgga attactatta ttacatattt atttttctgt gctggggtag aacccaggga 12241 actaagtact gtaataaccg agcttcattc atcctagtca tttttaattt tttttttttt 12301 ttttgttttt tcgagacaag gtttctctgt gtagccctgg ctgtcctgga actcactctg 12361 tagaccaggc tggcctcgaa ctcagaaatc tgcctgcctc tgcctcccag tgctgagatt 12421 aatgacgtgc accaccactg cctggcattt ttttaaagtt ttaatttgag accctttctc 12481 actaacttgc tcacactatc ctcgaactca ttacgtgggc tggtctagaa atttcagtca 12541 acttgcctca gcttctcaag tagctgggat tacaggcagg tgctaccact tctggccatg 12601 atcattgttg ttgttgctat tattattatt attatttcac tgatacttca tatcacaaca 12661 cagaatagta actgtagttc atgaccaaca aaaaagttgt acagtttctc tgttgtctta 12721 ttttgatctg gattttgatt tgtttcatgg cactgggcag gctaatctca aactgcatgg 12781 ctcaaatagt ccttctgtct taatctccca tgttctggaa gtataggtat cactacacct 12841 agctgtattt tcatatttta aaaaaaacgc ctaagaattg ctattgaatg aggcggagag 12901 atggctcagc agtcacaagt acttgtagag gactggggtt tggttactag cacctcaaag 12961 atggcttaca attatctgta gctccaattc cagaggatct gtgtggtaaa gctgcatgta 13021 tatgggcatg caggtaaaac tgctaaacat ataaaaatag ctcagtctta agagaattgg 13081 tcgactgtcg actttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 13141 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 13201 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 13261 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg 13321 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 13381 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 13441 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 13501 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 13561 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 13621 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 13681 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 13741 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 13801 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 13861 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 13921 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 13981 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 14041 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 14101 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 14161 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 14221 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 14281 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 14341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 14401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 14461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac caggtcgagt 14521 agggataaca gggtaatgga tcctcgttgg gcacctgcac tcatgtacgc aggcccacat 14581 atagatgtac acacgcatat cattcaaaat agaaattatt tatacaagta gtgcgttgga 14641 gagctggctc aggagttgtt aatgcttgct acccttgcaa aggacccaag ttcaatttcc 14701 agcacctgtg ttgggcatct cacaaccaca tgtaaatcta gtttcagggg atttcacccc 14761 tcttctgaac tcttcaggca cttataccca caaatggaca tctccacata gagacacata 14821 cttttaaaaa agtaatctag ggtctagaga aatggttata gaccacggac tgggatcagt 14881 tcccagaagc cacatcctgt ggctcacaat tgcctgtgaa tcaagttcta gatctggtgg 14941 cctcgtctag cctgcagtca tcaacaaaca catgcacaca tccatacaca cataatttaa 15001 aagaaagcga aagtaaccta taatgctaaa caaagcacat tctcagtgta gactgcagtg 15061 gtgatgaacc ttgaaaatat attaatagaa gccagacacg taagtcacaa atggtataac 15121 tcaatttata tgaaattccc agagagtcca aagttaaaaa aaacaaaaag gcagattatt 15181 ggttgtcagt agctacagag atgggacatt gaagatatta ataggtatag ggttgaggat 15241 tcatggcctt tccctcttcc atgttagcat gtctattgtt gttaacctta ttcaactcat 15301 gttagggcaa tcatattgat gagacttaat gagtatagct tgacattcct agaagacaga 15361 gtctcacatc agactctctg atcctctgac tcttagaatc tttcaacacc ctcttctgaa 15421 atgatctctg agcctttggt tgtgactgaa ttatgagaaa gaattttatg ttgaatgaat 15481 tttacttcag catttttaca aaactgaagc tggggatggc gtttcatgcg tgcagtccct 15541 accatttgcc tataagcctg gaggaggcag aagtagaatt aggcttttca gggacatctt 15601 cagctgcatc atgagcttga ggacaacttg agctacataa gattcccatc cccaccccca 15661 cacacacaca cacatctagt atgtacctag tatgcagtct tttcatatgt ttaaaatgta 15721 gaaaggtata tgtgaagtct cccttttccc cacaacttag tccctccaaa gtattgcatc 15781 agtttccttg tacatgattt tatgcatata taagatacac tatttctctc tctctctctc 15841 tccttccctc cttccctcca acctctgggt atgagttgag ggttatgtat gtttcaagtt 15901 gtaataagaa ttgccactca aacatttcat cccattttaa gttaagtttg tctgattttc 15961 cactttagaa gggcagccat gatggctcat gcctataatc ccatcctagc acttgtaagc 16021 ctaatacata agactggagg attgtcatca gcttgaggct agcctggtct acctaatgag 16081 ttccagccag ccaagagtag aatataagac cctgtctcca aaaaatgtac caaaggggct 16141 ggaggggtaa ctcagtggtt aagaacagta gctgctcttc cagaggacct gggttcaatt 16201 cccagcaccc acatggcagc ttgcaactat ctgtaactgt ccagctttgg gatatccgac 16261 accattatac caattacata aaatgaaaaa attgtaaaat gtgctaaaat gagtggggag 16321 ggggagcatg gtgtggttct gtgggcttgg gattttgcca tgaatccatg actatatctc 16381 agcaatatgt ggattatttc tggaaacctg tggggcatgg gtgtatggga agaggggaga 16441 gagagagagc ccgcatccag ccagagttcc tgtgctctgg gcaggcggac gtgggaggac 16501 tgctggacga tttccacttg gccctgggtg ggcatctggc tgtgtgaagc cactgacccc 16561 actcagcggg gggtagataa ggggcatttc ccataccagg ttctcagact ccagcattcc 16621 acgctggata cagcagaaga gctgagaaca gaataggggc cccagggaga cactctcgtc 16681 ccagagataa agaaggcaga gggagagaga ttcctacgca ggcgagaggc cttagtctgg 16741 gccttgatga acagagacgg tctatggttt tagagcttta ttgtagaaag gcagggggaa 16801 agagagaagg tagaaagaga gagaccagcc atggccaaga ggagagaagg ggggaaagga 16861 gagagagaag aaaggctaga gagtaagaaa ggttaaagag acagaagtaa aagcttagag 16921 taagagagag agagagagca agagggagag taagaaaagt aaaagcttaa gagagcgagg 16981 aggggccagc ccctctaata gtgggcttgt tatcttgttg ttgctaggta actgtgaagc 17041 gtctagcctg aaggccagaa gcttgggaca ttgtctatgt gactgaaagc catgagcctc 17101 tactgtgggg gctgtggagg tggtaacttg gacaggagcc aggggttcca ggagacatga 17161 gggaatgcct tctgtcccat gtaggtagga attaccacca ccggctttca ccgtagttca 17221 ctggagactg tttgtacata gcccattgcc ccacagaaac caggcactca ctgtttcatt 17281 tgcataagaa ttagaaggct ttattttggc tgggattggt gacttgcccc agaaattcca 17341 gcttttggga agccgaatcc tggagaacca ggagttcaag gtcatcctca ggtacctcat 17401 ttgttcaaga ccagcctgtg ctaccatgag accttgtccc tactcccctc aaccccaaaa 17461 cacgctgttt tcattttata ggttgtacta accatggagg atggtcattt gcctttctct 17521 tgatttcaga agcgtagcgc gggctttgca ccatgatcta gtgtttccag ataggcttgc 17581 ctctccacag ccaggcttat cctgcttcag tcccatgttt ggcaagggca acatatgact 17641 gaagaacttg catagcctta tggtgtatca tgactcttag ccccagttta taactgtcta 17701 acagctggtg gcttttgtga aaaaggaaag catcatttga acttttaact tttcagctgt 17761 ttgttttgac tttgcataaa tactgagact agctaggaaa tgagcttttg tttcatttgc 17821 taaaaatgat ttttttttca tgaggcaatt tcaagcaatt tgaatttagc aatctctact 17881 tgtctgaaaa gaagttatta attaaaaaaa aacaaacaaa caggaaccta ttatgctggc 17941 tagtcacatg aaagaatcca aatgaacaca gtcaaggctg aatcaacaca tgaaattatt 18001 ttctaattcc ccaaattaca tttatgctaa ttagtatgta gtaaaacatt ttaattgttt 18061 ccaatgttaa acattacaga aataattttg atgttttcct aataacccag cctttgatca 18121 tgtttgcatt tatttgattg ttgtcagttt attaaataat taagtaagag caacaagata 18181 aaatgacttc atgagagtcc ttttctgtaa cttagaacgt cagtagtcat aggaactgcg 18241 gtcgttccct tacctttcag aagtaggcta aagagttgaa cgcaaaggtg gtttttcgaa 18301 tcttcctgat ccttaagccc ccactaggtt tatattctat atttgatatc ggattcgaac 18361 ccctcgagct taggatccgg aacccttaat ataactaagt tattaggtcc ctcgaagagg 18421 ttcactagta ctggccattg cggccgcttc gaggctgcct cgcgcgtttc ggtgatgacg 18481 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 18541 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 18601 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 18661 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 18721 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 18781 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 18841 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 18901 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 18961 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 19021 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 19081 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 19141 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 19201 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 19261 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 19321 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 19381 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 19441 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 19501 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 19561 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 19621 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 19681 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 19741 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 19801 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 19861 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 19921 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 19981 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 20041 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 20101 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 20161 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 20221 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 20281 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 20341 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 20401 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 20461 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 20521 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 20581 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 20641 tccgcgcaca tttccccgaa aagtgccacc tgacgg //