LOCUS Ple51\(pEMS1362) 17507 bp DNA circular 17-NOV-2008 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2478 - 5' - GGAAACAAGTAGGGAGATGGTTGG- 3' Tm=56.8 Deg C Antisense primer: oEMS2474 - 5' - CCCTCCTCTAATTGGCACTAATGAC- 3' Tm=56.3 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 398bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|499191635| COMMENT VNTDBDATE|499191635| COMMENT LSOWNER| COMMENT VNTNAME|Ple51 (pEMS1362)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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14 (16 0) 1 2 0 0 Nil -326 100) COMMENT (CObjectList) (CObjectList)) COMMENT #194=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -256 100) COMMENT (CObjectList) (CObjectList)) COMMENT #195=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -116 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #196=(AnlzFontBackColorItem 935 1005 41976063) COMMENT #197=(AnlzFontBackColorItem 138 632 33606707) COMMENT #198=(AnlzFontBackColorItem 657 934 33606707) COMMENT #199=(AnlzFontBackColorItem 633 656 43633766) COMMENT #200=(AnlzFontBackColorItem 1031 1196 41976063) COMMENT #201=(AnlzFontBackColorItem 1006 1030 43633766))) (CObList) 1699618931 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers misc_recomb 11370..15181 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker complement(16280..17501) /vntifkey="22" /label=AmpR misc_marker 9919..11344 /vntifkey="22" /label=AmpR misc_recomb 6278..9923 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 2324..6277 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 11351..11368 /vntifkey="21" /label=I-Sce\I misc_feature 1206..1239 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 1240..1250 /vntifkey="21" /label=Kozak\consensus misc_feature 1247..1966 /vntifkey="21" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 1967..2002 /vntifkey="21" /label=Tetracysteine\residue misc_feature 2003..2075 /vntifkey="21" /label=NLS misc_feature 2081..2114 /vntifkey="21" /label=MFRT misc_feature 2268..2318 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..1197 /vntifkey="21" /label=Ple51\(DBH-D) misc_feature 936..1197 /vntifkey="21" /label=Prom\region misc_feature 139..935 /vntifkey="21" /label=Region\3 primer 634..657 /vntifkey="27" /label=oEMS2478 primer 1007..1031 /vntifkey="27" /label=oEMS2474 BASE COUNT 4671 a 3927 c 3929 g 4980 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccgc tgtgctcagc taacagtcgc ctgtggtttt tttttttttt 181 tcctggctgc ataagtaata ccttccgcca ctgttggagt tatctccaca tgcacagaga 241 agggtataag tcagcttggg ccttagcatg acacgggatt tattgctcat ttaaaccctg 301 aaagcaaatg gatttttgaa aactcctgaa gcatcaggga agtctgtctc ttcatgatta 361 tctggttgta tttatggatc ctttgccaaa tataaggacg ctcctgtcac ttcacccatc 421 ttgagatggg ctgcttttct ataaatatca aaatcccaca attatttcat gatttaatcc 481 ccatctgaat tttaaccaat tgcaacaccc tttttattat tatcctctga tgtaatcagc 541 ctggagatac aggagccgtt tgagtttgga tggtgtaggg gtgtcgaggg aagaaggcag 601 aggcaagagg agttgttttg gcctcatctt aaaggaaaca agtagggaga tggttgggcc 661 ccctggaagt gccctgcacc ccagtccaca gccctgggtt tgaacctcag cttggccact 721 tcccagccct gtggtcttgt gtggagtctt cagttcaggc tccatcctca gtttcttaat 781 ctgtgaaata gggctgatga ttctcactgt gcaaaacttc catgaaaggg gtaggttatg 841 cttagagcat ttttggcacc cagggaagat gttagtatct gtaccaccct cttggtttga 901 tcagaaaggg caatgcatgt acactggtga gaagagctct tcagagccat ctcagaaggg 961 acagcatccg cctgtctact tcaactccca ctgatgacgt ccatgtgtca ttagtgccaa 1021 ttagaggagg gcagcaggct gagtgcttgg cctggggcgc aagcttgtgg gagggaaaat 1081 tggattcccc gctagacaaa tgtgattacc cgtgctgcct ggacccaccc cattcaggac 1141 cagggcataa atggccaggt gggaccagag agctcacccc agccatgccc gccctcaggc 1201 gcgccgaagt tcctattctt caaaaggtat aggaacttcc gccaccatgg tgagcaaggg 1261 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 1321 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 1381 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 1441 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 1501 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 1561 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 1621 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 1681 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 1741 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 1801 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 1861 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 1921 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagttatttc tgaactgctg 1981 cccgggctgc tgcatggaac cggatccaaa aaagaagaga aaggtagatc caaaaaagaa 2041 gagaaaggta gatccaaaaa agaagagaaa ggtaatataa gaagttccta ttctctagaa 2101 agtataggaa cttcagcggc cgcgactcta gatcataatc agccatacca catttgtaga 2161 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 2221 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 2281 catcacaaat ttcacaaata aagcattttt ttcactgcga attctcgtaa aactcttcat 2341 ggcagtagtt attattctct ctctctctct ttttcttttt tcttgagaca ggatatttct 2401 ctgttgccca ggctggagtg cagtggcaca gtcttggctc actgcagcct ggacctcctg 2461 ggctcaagcc atcctcccac ctcagcctcc caagtagctg gggctacagg cacatggcca 2521 ccaggcccag ataatttttc atttttgtag agactgagtc tcaccatgtt acccaggttt 2581 attattctca ttttttagat gaagagactg aggtccagag aagctcaatg acttgcctag 2641 ttttacaaat ctcctgccat cacatacccc tcagcgtcct taataagagg gaggccacca 2701 actatgtgct gggcactgtg gtggatgctg gagctatagg gttgagtata taagaaatgg 2761 tgttgctgga gcaactgttg cttgcttacc tgacctatct gagaattaat tagcagggga 2821 acatattttt gttttcagat tcaatataag aacttgtgtg ggcaaaaata aagatcagta 2881 gtaataacag tagttcccat ttgctgactg tactgtccta agtgcatata tatatacata 2941 cacacacgca tacctatact cctctaatac tcaaaatgat cctgtttatg tattgttaat 3001 atgctcattt tatttttaaa tttttattta tttttatttt tatttatttt tgagacggag 3061 tctcattctg tcgcggaggc tgaagtgcag tggtgcgatc tcagctcagt gcgacctccg 3121 cctcccgggt tcaagtgatt ctcctgcctc agcctccgga ctagctggga ttacaggcgc 3181 ccgcctccac gcccagctaa tttttgtatt tttagtagag atggggtttc gccatgttgg 3241 ccaggctggt ctcgtactcc tgaccttgag tgatccacct gcctcggcct cccaaagtgc 3301 tgggattaca ggcatgagcc accgcgccgg gctaatatgc tcattttagt gaggcaaaaa 3361 tagaggctca gagtctgatt tgtacaaaac tacagagcag ttaagtgtcc tctcagatgt 3421 gtaccctgat ctgggtgact ctaggactct aggtctcaac tgttacaacc agttaagggt 3481 ttggggaagc actgggccaa gagtcaggaa aatggaagcc acaggtagtg caaggtcttg 3541 ggaatgggac gtctggtcca aggattcacg cgatgactgg aacccgaaga gccggggccc 3601 ggtttacggc cgccatgaag caacgcgcgc cggtaggttt gggaatcagg gagccctctg 3661 aataggagac tgagttggga gggaaagggg cttcgctggg ggagcctcgg cttcttctgg 3721 gagaaaattc ccacggctac ctagtgagcc tgcaaactgg taggcgccgg cgtaggcgcg 3781 cgggcggggc cgggggcggg gcctgcgggg cgtggcgggg cgggcagagg gcggggcctg 3841 cttctcctca gcttcaggcg gctgcgacga gccctcaggc gaacctctcg gctttcccgc 3901 gcggcgccgc ctcttgctgc gcctccgcct cctcctctgc tccgccaccg gcttcctcct 3961 cctgagcagt cagcccgcgc gccggccggc tccgttatgg cgacccgcag ccctggcgtc 4021 gtggtgagca gctcggcctg ccggccctgg ccggttcagg cccacgcggc aggtggcggc 4081 cgggccctga ggcgcgggat cgatccatga cacccccccg ccccccatat ctcactataa 4141 ttcactgtca aatggctttt atgatttgga gtttggggat acttttaaag acagggtctt 4201 ggtagctcct agatggcttg ggattcactt agtaaccctt gctggcctaa aattcaggaa 4261 cttctacctc atcttcccaa gtactgggtt agtagggatg tccagcatat ttagcttgct 4321 ttaatgattt tgctgtactt ttttctgtgt gtacaggtgc atgtatattt ataaaactaa 4381 cataatatat tttaccccca aagtaccata accctaatta atgctattaa atgttttgta 4441 tgctgggctc tgggcatact aatataacca atattataca atgcaaaaca attttggaat 4501 atcttctgta aaattatctt attagtaagt tgtaaccatc tcagacttct aagtttgagt 4561 ttgtaaagga tcagtttttt ttggtgacct acacacacac acacacacac acacacacac 4621 acacacacag gggggtggat tctgagaatt tagagaattt agatggttaa taccagtttt 4681 tatttttatt ctgtgtgata tttatttatt aagatttatt tatttattac atgtaggtac 4741 actgtatctg tcttcagaca caccagaaga gggcatcaga tctcgttaca gatggttgtg 4801 agtcaccatg tggttgctgg gatttgaact caggacctct ggaagaacag tcagtgctct 4861 taaccgttga gccatctcac cagccccgtg atattttatt taacttacta gaatatactg 4921 tgttaaaaca tattttaggg gattggggag atggcccatt ctgtaaagtg tctgccatgt 4981 catcttcagg acctgcgttt gtaaccccag catggagaag acagagacag ggagacccct 5041 gggcttcctg gcaagctaga ctagcaaatt tggttaacta ggttcaatga gagactcagt 5101 ctcaaaacat aagaagtgaa ttttttagac ccaatttcaa tctctgaact ccatacgtat 5161 gcacatgtac ctgcatataa atatatatac acacccataa acacatgcac acacaaaaaa 5221 tggttcaaaa gttcttaaaa tgtataagag tagaaagtgt agagccggga gtggtggcgc 5281 acgcctttaa tcccagcact tgggagcaga ggcaggtgga tttctgagtt cgaggccagc 5341 ctggtctaca gagtgagttc caggacagcc agagctatac agagaaatcc tgcctcaaaa 5401 aaccaaaaat aaagtgtata tactaatctt tcatgcctaa aatatttatt tttgccacat 5461 ttttcatacc tctttaatat ttttcttttt ctctttgtgc ttcaaaggaa attttgtgtc 5521 atgttttact tttcattctt gactctattc agcagtaaga cgcagcatgg ctttcttaga 5581 gaagtataat ctattacaaa acggtaacat ttaactgctc tacataccct cttgtagcta 5641 aattcctctg ttaaactaag acttattatc taaacgtaat taaatgttga tatcttataa 5701 tgctccactt tgaaacagct gtctttcttt tgcagattag cgatgatgaa ccaggttatg 5761 acctagattt gttttgtata cctaatcatt atgccgagga tttggaaaaa gtgtttattc 5821 ctcatggact gattatggac aggttagtaa gacctcgatt gaagttattt ttagagggtg 5881 tgtgtgtggt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtaa gagagagagg 5941 agaggcatgt gtgatatata tgtgaagtgt attgcatgta catggaatcc agagaaggac 6001 attaggtgtc ctactctgtt gctctcttat tcccttgaat cagtgtctct tgctgaacct 6061 ggggcataca tactgatttg gctaggctgc ctggctagca agacccaggg atctgcttgt 6121 ctctgccccc cagtgctgac gttacaagca ggtgcaacca catctggttt ttatatgggt 6181 actgggggat ctgaacttag ggcctgatgc gtgcccaagt gctctcaccc gccaagccac 6241 ctctccagcc aaagggtttt ttttcttgta ttaattctaa cttagcaact gttgagtagt 6301 tgatgtttga atataagcta agccaactta ttagaataaa tgttttctat tttttacttg 6361 tatgtgtgag tgcctgcatg tatgtatgtg aagcatatgt attccaggat gtcagaattg 6421 tgatggattc cctggaactg gagttacaga ccattgtgaa ccactgtgta tgggtgctgg 6481 gaactgaact ttaggtcctc tgcaaaaaca gtaaggatct taactgataa actatctttc 6541 tagccctaag gctttctact tttaaagttc gtttctcttt ctctctctct ctctctctct 6601 ctctctctct ctccctctcc ctccctcctc ctcctcctcc tctttccccc ctctcgcctc 6661 ctctttctct ctcccctccc tctctctctc tcacacacac acacagacag agagaggaga 6721 gagaaaggaa gccttactaa ttaattacct agttttagac ataactagtg ggaatccata 6781 gtaagtaaat caacattctt gaagagaggt tagaagaatt ggctctcgtt gtccatcgag 6841 gctgtataag attattcttc tgttttctca gcttaccata catattttct acgtagacaa 6901 taccaattca aatacactgt acgtaaagta tatgcgcaca cacacacaca cacacacaca 6961 cacacacaca caagttacat attgtcatat agtactaaaa tttatggact ttttcatatt 7021 aatacatcta gagctatcat gttttttatc ttttaaaaat tactttacgg ggctacagag 7081 atgtctcagc agttaggagc actgactgct cttccagagg tcctgagttc aattcccagc 7141 aaccacatgg tggctcacaa ccatctgtaa tgggatctga tccactctta tggtgtgtct 7201 gaagacagtg acagtgtact catatacatg aaataaatct ttaaaaaatt actttaccat 7261 cagggtatat atatatatat atatgtgtgt gtgtgtgtgt gtgtgtatga agtatttgct 7321 atttgagagg gtctcactgt ttagctcagt ctggcttgga acgttctgta tagcccagac 7381 tggcctcaac tcagcagtcc ccctgaaagg ctctcatatg tggagattac aggtatgtgc 7441 tactgtgccc agccctacat gttactttgt ttttgaaata gggtatcaaa tagcttagac 7501 tggcttctaa gtcagtgtgt agctaaggat ggtctgagct tctgaacctg ctacttcaac 7561 tcctggtgtg ccatcatggc cagtttgcac agttgtgaga gtagaactca ggacctccta 7621 tgtgttacta gacatgttct ctaccagctg agctacatcc caattcgcct acatttactt 7681 ttatttttta aaagatttat ttatgtatac agcactctgc ctgcatgtac atctgcatgc 7741 cagaagaggg caccagatct catcatacat agttgtgagc caccatgtgg ttgctgggga 7801 ttgaactcag ggcctctgga agagcagtca gtgctcttaa cctctgagcc atctctgtag 7861 ccccctacat ttattttaaa gattataggc tattctattc atagatgtgc cataactgat 7921 ttaaccagat cttcattatt ttgtacttag tagagtatat tagagggaat gaatcttaaa 7981 tacacatttt gacacttgct cagctttaga caagtaaaaa tcactggtca aggaaatggt 8041 gcttagtgga tgcttctgaa ctgtcaaatt gtcctccaac aagttacatc attttatact 8101 tccaacgtta tgtgagagtt catttttctt taggctcttc tagtatttta tatttgagaa 8161 ataaaattta accaaaattt gccaatctag taagcttgtt tttatttgta tctttaaatt 8221 ttatgataga tgttatagtg tactctcctc tcccctcccc tcccctctcc tcctcagcag 8281 gatcttgtta tgtagcccta agggatctaa tacttactct gtagtccagg ctgtccttca 8341 gctcatgaga cacaccatca tgcttggcat gtttcttgtt ctgaaaggta tctggtttta 8401 attttggttg agctggtgtg ttggtgtata tctgtaatct cagcacaggc aagtagagac 8461 aaggattatg attacagtcc agcctgggct atttagaggg attctcttta atctttcctc 8521 atgccccaaa atcttacctt tggtatatga aaaatagtct ccacttctgc aaaatattgc 8581 tttatgaagt aagaattaat tcccttcata gagacaagga atgtgtcctg taaaagttta 8641 atgtgtaaga agtatttgtt ataaaagata aatattcaga atcttctttt taattcctga 8701 ttttatttct ataggactga aagacttgct cgagatgtca tgaaggagat gggaggccat 8761 cacattgtgg ccctctgtgt gctcaagggg ggctataagt tctttgctga cctgctggat 8821 tacattaaag cactgaatag aaatagtgat agatccattc ctatgactgt agattttatc 8881 agactgaaga gctactgtgt aagtataatt aacttataat taaaaaaata gggccattct 8941 agttttatct atattttttt taaacttgtg caaactatgc tacataatca acttacttga 9001 ttatgtaata tatatagtaa tagttggaat tactattatt acatatttat ttttctgtgc 9061 tggggtagaa cccagggaac taagtactgt aataaccgag cttcattcat cctagtcatt 9121 tttaattttt tttttttttt ttgttttttc gagacaaggt ttctctgtgt agccctggct 9181 gtcctggaac tcactctgta gaccaggctg gcctcgaact cagaaatctg cctgcctctg 9241 cctcccagtg ctgagattaa tgacgtgcac caccactgcc tggcattttt ttaaagtttt 9301 aatttgagac cctttctcac taacttgctc acactatcct cgaactcatt acgtgggctg 9361 gtctagaaat ttcagtcaac ttgcctcagc ttctcaagta gctgggatta caggcaggtg 9421 ctaccacttc tggccatgat cattgttgtt gttgctatta ttattattat tatttcactg 9481 atacttcata tcacaacaca gaatagtaac tgtagttcat gaccaacaaa aaagttgtac 9541 agtttctctg ttgtcttatt ttgatctgga ttttgatttg tttcatggca ctgggcaggc 9601 taatctcaaa ctgcatggct caaatagtcc ttctgtctta atctcccatg ttctggaagt 9661 ataggtatca ctacacctag ctgtattttc atattttaaa aaaaacgcct aagaattgct 9721 attgaatgag gcggagagat ggctcagcag tcacaagtac ttgtagagga ctggggtttg 9781 gttactagca cctcaaagat ggcttacaat tatctgtagc tccaattcca gaggatctgt 9841 gtggtaaagc tgcatgtata tgggcatgca ggtaaaactg ctaaacatat aaaaatagct 9901 cagtcttaag agaattggtc gactttttcg ccctttgacg ttggagtcca cgttctttaa 9961 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 10021 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 10081 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 10141 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 10201 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 10261 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 10321 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 10381 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 10441 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 10501 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 10561 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 10621 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 10681 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 10741 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 10801 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 10861 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 10921 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 10981 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 11041 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 11101 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 11161 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 11221 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 11281 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 11341 ccaggtcgag tagggataac agggtaatgg atcctcgttg ggcacctgca ctcatgtacg 11401 caggcccaca tatagatgta cacacgcata tcattcaaaa tagaaattat ttatacaagt 11461 agtgcgttgg agagctggct caggagttgt taatgcttgc tacccttgca aaggacccaa 11521 gttcaatttc cagcacctgt gttgggcatc tcacaaccac atgtaaatct agtttcaggg 11581 gatttcaccc ctcttctgaa ctcttcaggc acttataccc acaaatggac atctccacat 11641 agagacacat acttttaaaa aagtaatcta gggtctagag aaatggttat agaccacgga 11701 ctgggatcag ttcccagaag ccacatcctg tggctcacaa ttgcctgtga atcaagttct 11761 agatctggtg gcctcgtcta gcctgcagtc atcaacaaac acatgcacac atccatacac 11821 acataattta aaagaaagcg aaagtaacct ataatgctaa acaaagcaca ttctcagtgt 11881 agactgcagt ggtgatgaac cttgaaaata tattaataga agccagacac gtaagtcaca 11941 aatggtataa ctcaatttat atgaaattcc cagagagtcc aaagttaaaa aaaacaaaaa 12001 ggcagattat tggttgtcag tagctacaga gatgggacat tgaagatatt aataggtata 12061 gggttgagga ttcatggcct ttccctcttc catgttagca tgtctattgt tgttaacctt 12121 attcaactca tgttagggca atcatattga tgagacttaa tgagtatagc ttgacattcc 12181 tagaagacag agtctcacat cagactctct gatcctctga ctcttagaat ctttcaacac 12241 cctcttctga aatgatctct gagcctttgg ttgtgactga attatgagaa agaattttat 12301 gttgaatgaa ttttacttca gcatttttac aaaactgaag ctggggatgg cgtttcatgc 12361 gtgcagtccc taccatttgc ctataagcct ggaggaggca gaagtagaat taggcttttc 12421 agggacatct tcagctgcat catgagcttg aggacaactt gagctacata agattcccat 12481 ccccaccccc acacacacac acacatctag tatgtaccta gtatgcagtc ttttcatatg 12541 tttaaaatgt agaaaggtat atgtgaagtc tcccttttcc ccacaactta gtccctccaa 12601 agtattgcat cagtttcctt gtacatgatt ttatgcatat ataagataca ctatttctct 12661 ctctctctct ctccttccct ccttccctcc aacctctggg tatgagttga gggttatgta 12721 tgtttcaagt tgtaataaga attgccactc aaacatttca tcccatttta agttaagttt 12781 gtctgatttt ccactttaga agggcagcca tgatggctca tgcctataat cccatcctag 12841 cacttgtaag cctaatacat aagactggag gattgtcatc agcttgaggc tagcctggtc 12901 tacctaatga gttccagcca gccaagagta gaatataaga ccctgtctcc aaaaaatgta 12961 ccaaaggggc tggaggggta actcagtggt taagaacagt agctgctctt ccagaggacc 13021 tgggttcaat tcccagcacc cacatggcag cttgcaacta tctgtaactg tccagctttg 13081 ggatatccga caccattata ccaattacat aaaatgaaaa aattgtaaaa tgtgctaaaa 13141 tgagtgggga gggggagcat ggtgtggttc tgtgggcttg ggattttgcc atgaatccat 13201 gactatatct cagcaatatg tggattattt ctggaaacct gtggggcatg ggtgtatggg 13261 aagaggggag agagagagag cccgcatcca gccagagttc ctgtgctctg ggcaggcgga 13321 cgtgggagga ctgctggacg atttccactt ggccctgggt gggcatctgg ctgtgtgaag 13381 ccactgaccc cactcagcgg ggggtagata aggggcattt cccataccag gttctcagac 13441 tccagcattc cacgctggat acagcagaag agctgagaac agaatagggg ccccagggag 13501 acactctcgt cccagagata aagaaggcag agggagagag attcctacgc aggcgagagg 13561 ccttagtctg ggccttgatg aacagagacg gtctatggtt ttagagcttt attgtagaaa 13621 ggcaggggga aagagagaag gtagaaagag agagaccagc catggccaag aggagagaag 13681 gggggaaagg agagagagaa gaaaggctag agagtaagaa aggttaaaga gacagaagta 13741 aaagcttaga gtaagagaga gagagagagc aagagggaga gtaagaaaag taaaagctta 13801 agagagcgag gaggggccag cccctctaat agtgggcttg ttatcttgtt gttgctaggt 13861 aactgtgaag cgtctagcct gaaggccaga agcttgggac attgtctatg tgactgaaag 13921 ccatgagcct ctactgtggg ggctgtggag gtggtaactt ggacaggagc caggggttcc 13981 aggagacatg agggaatgcc ttctgtccca tgtaggtagg aattaccacc accggctttc 14041 accgtagttc actggagact gtttgtacat agcccattgc cccacagaaa ccaggcactc 14101 actgtttcat ttgcataaga attagaaggc tttattttgg ctgggattgg tgacttgccc 14161 cagaaattcc agcttttggg aagccgaatc ctggagaacc aggagttcaa ggtcatcctc 14221 aggtacctca tttgttcaag accagcctgt gctaccatga gaccttgtcc ctactcccct 14281 caaccccaaa acacgctgtt ttcattttat aggttgtact aaccatggag gatggtcatt 14341 tgcctttctc ttgatttcag aagcgtagcg cgggctttgc accatgatct agtgtttcca 14401 gataggcttg cctctccaca gccaggctta tcctgcttca gtcccatgtt tggcaagggc 14461 aacatatgac tgaagaactt gcatagcctt atggtgtatc atgactctta gccccagttt 14521 ataactgtct aacagctggt ggcttttgtg aaaaaggaaa gcatcatttg aacttttaac 14581 ttttcagctg tttgttttga ctttgcataa atactgagac tagctaggaa atgagctttt 14641 gtttcatttg ctaaaaatga tttttttttc atgaggcaat ttcaagcaat ttgaatttag 14701 caatctctac ttgtctgaaa agaagttatt aattaaaaaa aaacaaacaa acaggaacct 14761 attatgctgg ctagtcacat gaaagaatcc aaatgaacac agtcaaggct gaatcaacac 14821 atgaaattat tttctaattc cccaaattac atttatgcta attagtatgt agtaaaacat 14881 tttaattgtt tccaatgtta aacattacag aaataatttt gatgttttcc taataaccca 14941 gcctttgatc atgtttgcat ttatttgatt gttgtcagtt tattaaataa ttaagtaaga 15001 gcaacaagat aaaatgactt catgagagtc cttttctgta acttagaacg tcagtagtca 15061 taggaactgc ggtcgttccc ttacctttca gaagtaggct aaagagttga acgcaaaggt 15121 ggtttttcga atcttcctga tccttaagcc cccactaggt ttatattcta tatttgatat 15181 cggattcgaa cccctcgagc ttaggatccg gaacccttaa tataactaag ttattaggtc 15241 cctcgaagag gttcactagt actggccatt gcggccgctt cgaggctgcc tcgcgcgttt 15301 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 15361 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 15421 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat 15481 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 15541 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 15601 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 15661 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 15721 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 15781 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 15841 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 15901 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 15961 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 16021 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 16081 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 16141 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 16201 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 16261 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 16321 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 16381 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 16441 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 16501 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 16561 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 16621 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 16681 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 16741 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 16801 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt 16861 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 16921 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 16981 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 17041 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 17101 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact 17161 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 17221 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 17281 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 17341 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 17401 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 17461 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgg //