LOCUS Ple148\(pEMS1169 17491 bp DNA circular 17-NOV-2008 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2364 5'- GCGTATCACGAGGCCCTTTC-3' Tm= 56.0 Deg C Antisense primer: oEMS2674 5'- TGGTCAGGCTGCAACTCACG-3' Tm=56.9 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 796 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|499183119| COMMENT VNTDBDATE|499183158| COMMENT LSOWNER| COMMENT VNTNAME|Ple148 (pEMS1169)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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0 Nil -325 100) COMMENT (CObjectList) (CObjectList)) COMMENT #192=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -325 100) COMMENT (CObjectList) (CObjectList)) COMMENT #193=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -186 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #194=(AnlzFontBackColorItem 138 709 45977377) COMMENT #195=(AnlzFontBackColorItem 710 807 41976063) COMMENT #196=(AnlzFontBackColorItem 53 72 43633766) COMMENT #197=(AnlzFontBackColorItem 828 1180 41976063) COMMENT #198=(AnlzFontBackColorItem 808 827 43633766))) (CObList) 17498130 COMMENT (CStringList) 0 327734 (CObList))) FEATURES Location/Qualifiers misc_recomb 11354..15165 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker complement(16264..17485) /vntifkey="22" /label=AmpR misc_marker 9903..11328 /vntifkey="22" /label=AmpR misc_recomb 6262..9907 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 2308..6261 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 11335..11352 /vntifkey="21" /label=I-Sce\I misc_feature 1190..1223 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 1224..1234 /vntifkey="21" /label=Kozak\consensus misc_feature 1231..1950 /vntifkey="21" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 1951..1986 /vntifkey="21" /label=Tetracysteine\residue misc_feature 1987..2059 /vntifkey="21" /label=NLS misc_feature 2065..2098 /vntifkey="21" /label=MFRT misc_feature 2252..2302 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 139..710 /vntifkey="21" /label=region\9 misc_feature 711..1181 /vntifkey="21" /label=Prom\region primer 54..73 /vntifkey="27" /label=oEMS2364 primer 809..828 /vntifkey="27" /label=oEMS2674 misc_feature 139..1181 /vntifkey="21" /label=Ple148\(OLIG1-A) BASE COUNT 4660 a 4011 c 3951 g 4869 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggccgc ttcacagcag tacaccccat ctttgttctt acagaggaga 181 gggatggtgc tacatctgaa acacactcac acatcactga ggctttgagt ccccagtgtc 241 tgccatttcc aaggtccaag cttcttgacc caaagcaaga gtagatggca gggacagggg 301 aatgcgcagg agacactagg tttctacaca aggaaaagga aatctgaggc acacacctct 361 gcagagtgaa aggaatcacc aacacacaca cacaagcccc tttaggcaaa gaaaagagcg 421 gttgttatgg tggacacaag ttgctcccca agcatgtcct caatgtggga ggccgcagca 481 gctgcctccc cttgagacag accccggggc ccgtgccagc ggccacagta actggctggc 541 ccttgccagc tgtcacactt tgcatgccgc tgcttcagcc cagcacaagg gaccatccaa 601 gtcaccagct ccaaccagtc ccaaatcccg tgcccaggct ggagctggat ggccacccag 661 aaccctcttc tgaggccctc taccaaggca ctgaaatatg cagcatatcc acataagacg 721 gtaaatttta tgttatgaat atgtgatcac agttaaaaaa aaatatgagg gaggttgttt 781 ttgagtagac agttgctgga ggaaggcacg tgagttgcag cctgaccagt gcgaccagtc 841 ctccttcaac agccccgctt gccttgaaaa ggcgccctta ctccgctcag ggggcctgtc 901 ccgcgcggct ggcacaggcg ctcacaataa cctcctaaag cggtgccagc cgcacctccg 961 cgcggccccg gcacaagcag ccaatgaaca cgcggctgcg cccggcctcg cgcctccatt 1021 ggctgcgccc cgccacccgc tgccccgcag gttcccaagc cgggtttaaa ggggtagggc 1081 gcgggccagg gccccaccat cgtttccccg cgcgcaggtc cgcggggagg ggcggcctgc 1141 cgaccggccc accccagggc gttcctgaag ggcgtcctcg gggcgcgccg aagttcctat 1201 tcttcaaaag gtataggaac ttccgccacc atggtgagca agggcgagga gctgttcacc 1261 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 1321 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 1381 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 1441 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 1501 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 1561 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 1621 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 1681 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 1741 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 1801 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 1861 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 1921 actctcggca tggacgagct gtacaagtta tttctgaact gctgcccggg ctgctgcatg 1981 gaaccggatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtagatcca 2041 aaaaagaaga gaaaggtaat ataagaagtt cctattctct agaaagtata ggaacttcag 2101 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta 2161 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 2221 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 2281 aataaagcat ttttttcact gcgaattctc gtaaaactct tcatggcagt agttattatt 2341 ctctctctct ctctttttct tttttcttga gacaggatat ttctctgttg cccaggctgg 2401 agtgcagtgg cacagtcttg gctcactgca gcctggacct cctgggctca agccatcctc 2461 ccacctcagc ctcccaagta gctggggcta caggcacatg gccaccaggc ccagataatt 2521 tttcattttt gtagagactg agtctcacca tgttacccag gtttattatt ctcatttttt 2581 agatgaagag actgaggtcc agagaagctc aatgacttgc ctagttttac aaatctcctg 2641 ccatcacata cccctcagcg tccttaataa gagggaggcc accaactatg tgctgggcac 2701 tgtggtggat gctggagcta tagggttgag tatataagaa atggtgttgc tggagcaact 2761 gttgcttgct tacctgacct atctgagaat taattagcag gggaacatat ttttgttttc 2821 agattcaata taagaacttg tgtgggcaaa aataaagatc agtagtaata acagtagttc 2881 ccatttgctg actgtactgt cctaagtgca tatatatata catacacaca cgcataccta 2941 tactcctcta atactcaaaa tgatcctgtt tatgtattgt taatatgctc attttatttt 3001 taaattttta tttattttta tttttattta tttttgagac ggagtctcat tctgtcgcgg 3061 aggctgaagt gcagtggtgc gatctcagct cagtgcgacc tccgcctccc gggttcaagt 3121 gattctcctg cctcagcctc cggactagct gggattacag gcgcccgcct ccacgcccag 3181 ctaatttttg tatttttagt agagatgggg tttcgccatg ttggccaggc tggtctcgta 3241 ctcctgacct tgagtgatcc acctgcctcg gcctcccaaa gtgctgggat tacaggcatg 3301 agccaccgcg ccgggctaat atgctcattt tagtgaggca aaaatagagg ctcagagtct 3361 gatttgtaca aaactacaga gcagttaagt gtcctctcag atgtgtaccc tgatctgggt 3421 gactctagga ctctaggtct caactgttac aaccagttaa gggtttgggg aagcactggg 3481 ccaagagtca ggaaaatgga agccacaggt agtgcaaggt cttgggaatg ggacgtctgg 3541 tccaaggatt cacgcgatga ctggaacccg aagagccggg gcccggttta cggccgccat 3601 gaagcaacgc gcgccggtag gtttgggaat cagggagccc tctgaatagg agactgagtt 3661 gggagggaaa ggggcttcgc tgggggagcc tcggcttctt ctgggagaaa attcccacgg 3721 ctacctagtg agcctgcaaa ctggtaggcg ccggcgtagg cgcgcgggcg gggccggggg 3781 cggggcctgc ggggcgtggc ggggcgggca gagggcgggg cctgcttctc ctcagcttca 3841 ggcggctgcg acgagccctc aggcgaacct ctcggctttc ccgcgcggcg ccgcctcttg 3901 ctgcgcctcc gcctcctcct ctgctccgcc accggcttcc tcctcctgag cagtcagccc 3961 gcgcgccggc cggctccgtt atggcgaccc gcagccctgg cgtcgtggtg agcagctcgg 4021 cctgccggcc ctggccggtt caggcccacg cggcaggtgg cggccgggcc ctgaggcgcg 4081 ggatcgatcc atgacacccc cccgcccccc atatctcact ataattcact gtcaaatggc 4141 ttttatgatt tggagtttgg ggatactttt aaagacaggg tcttggtagc tcctagatgg 4201 cttgggattc acttagtaac ccttgctggc ctaaaattca ggaacttcta cctcatcttc 4261 ccaagtactg ggttagtagg gatgtccagc atatttagct tgctttaatg attttgctgt 4321 acttttttct gtgtgtacag gtgcatgtat atttataaaa ctaacataat atattttacc 4381 cccaaagtac cataacccta attaatgcta ttaaatgttt tgtatgctgg gctctgggca 4441 tactaatata accaatatta tacaatgcaa aacaattttg gaatatcttc tgtaaaatta 4501 tcttattagt aagttgtaac catctcagac ttctaagttt gagtttgtaa aggatcagtt 4561 ttttttggtg acctacacac acacacacac acacacacac acacacacac acaggggggt 4621 ggattctgag aatttagaga atttagatgg ttaataccag tttttatttt tattctgtgt 4681 gatatttatt tattaagatt tatttattta ttacatgtag gtacactgta tctgtcttca 4741 gacacaccag aagagggcat cagatctcgt tacagatggt tgtgagtcac catgtggttg 4801 ctgggatttg aactcaggac ctctggaaga acagtcagtg ctcttaaccg ttgagccatc 4861 tcaccagccc cgtgatattt tatttaactt actagaatat actgtgttaa aacatatttt 4921 aggggattgg ggagatggcc cattctgtaa agtgtctgcc atgtcatctt caggacctgc 4981 gtttgtaacc ccagcatgga gaagacagag acagggagac ccctgggctt cctggcaagc 5041 tagactagca aatttggtta actaggttca atgagagact cagtctcaaa acataagaag 5101 tgaatttttt agacccaatt tcaatctctg aactccatac gtatgcacat gtacctgcat 5161 ataaatatat atacacaccc ataaacacat gcacacacaa aaaatggttc aaaagttctt 5221 aaaatgtata agagtagaaa gtgtagagcc gggagtggtg gcgcacgcct ttaatcccag 5281 cacttgggag cagaggcagg tggatttctg agttcgaggc cagcctggtc tacagagtga 5341 gttccaggac agccagagct atacagagaa atcctgcctc aaaaaaccaa aaataaagtg 5401 tatatactaa tctttcatgc ctaaaatatt tatttttgcc acatttttca tacctcttta 5461 atatttttct ttttctcttt gtgcttcaaa ggaaattttg tgtcatgttt tacttttcat 5521 tcttgactct attcagcagt aagacgcagc atggctttct tagagaagta taatctatta 5581 caaaacggta acatttaact gctctacata ccctcttgta gctaaattcc tctgttaaac 5641 taagacttat tatctaaacg taattaaatg ttgatatctt ataatgctcc actttgaaac 5701 agctgtcttt cttttgcaga ttagcgatga tgaaccaggt tatgacctag atttgttttg 5761 tatacctaat cattatgccg aggatttgga aaaagtgttt attcctcatg gactgattat 5821 ggacaggtta gtaagacctc gattgaagtt atttttagag ggtgtgtgtg tggtgtgtgt 5881 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtaagagaga gaggagaggc atgtgtgata 5941 tatatgtgaa gtgtattgca tgtacatgga atccagagaa ggacattagg tgtcctactc 6001 tgttgctctc ttattccctt gaatcagtgt ctcttgctga acctggggca tacatactga 6061 tttggctagg ctgcctggct agcaagaccc agggatctgc ttgtctctgc cccccagtgc 6121 tgacgttaca agcaggtgca accacatctg gtttttatat gggtactggg ggatctgaac 6181 ttagggcctg atgcgtgccc aagtgctctc acccgccaag ccacctctcc agccaaaggg 6241 ttttttttct tgtattaatt ctaacttagc aactgttgag tagttgatgt ttgaatataa 6301 gctaagccaa cttattagaa taaatgtttt ctatttttta cttgtatgtg tgagtgcctg 6361 catgtatgta tgtgaagcat atgtattcca ggatgtcaga attgtgatgg attccctgga 6421 actggagtta cagaccattg tgaaccactg tgtatgggtg ctgggaactg aactttaggt 6481 cctctgcaaa aacagtaagg atcttaactg ataaactatc tttctagccc taaggctttc 6541 tacttttaaa gttcgtttct ctttctctct ctctctctct ctctctctct ctctctccct 6601 ctccctccct cctcctcctc ctcctctttc ccccctctcg cctcctcttt ctctctcccc 6661 tccctctctc tctctcacac acacacacag acagagagag gagagagaaa ggaagcctta 6721 ctaattaatt acctagtttt agacataact agtgggaatc catagtaagt aaatcaacat 6781 tcttgaagag aggttagaag aattggctct cgttgtccat cgaggctgta taagattatt 6841 cttctgtttt ctcagcttac catacatatt ttctacgtag acaataccaa ttcaaataca 6901 ctgtacgtaa agtatatgcg cacacacaca cacacacaca cacacacaca cacacaagtt 6961 acatattgtc atatagtact aaaatttatg gactttttca tattaataca tctagagcta 7021 tcatgttttt tatcttttaa aaattacttt acggggctac agagatgtct cagcagttag 7081 gagcactgac tgctcttcca gaggtcctga gttcaattcc cagcaaccac atggtggctc 7141 acaaccatct gtaatgggat ctgatccact cttatggtgt gtctgaagac agtgacagtg 7201 tactcatata catgaaataa atctttaaaa aattacttta ccatcagggt atatatatat 7261 atatatatgt gtgtgtgtgt gtgtgtgtgt atgaagtatt tgctatttga gagggtctca 7321 ctgtttagct cagtctggct tggaacgttc tgtatagccc agactggcct caactcagca 7381 gtccccctga aaggctctca tatgtggaga ttacaggtat gtgctactgt gcccagccct 7441 acatgttact ttgtttttga aatagggtat caaatagctt agactggctt ctaagtcagt 7501 gtgtagctaa ggatggtctg agcttctgaa cctgctactt caactcctgg tgtgccatca 7561 tggccagttt gcacagttgt gagagtagaa ctcaggacct cctatgtgtt actagacatg 7621 ttctctacca gctgagctac atcccaattc gcctacattt acttttattt tttaaaagat 7681 ttatttatgt atacagcact ctgcctgcat gtacatctgc atgccagaag agggcaccag 7741 atctcatcat acatagttgt gagccaccat gtggttgctg gggattgaac tcagggcctc 7801 tggaagagca gtcagtgctc ttaacctctg agccatctct gtagccccct acatttattt 7861 taaagattat aggctattct attcatagat gtgccataac tgatttaacc agatcttcat 7921 tattttgtac ttagtagagt atattagagg gaatgaatct taaatacaca ttttgacact 7981 tgctcagctt tagacaagta aaaatcactg gtcaaggaaa tggtgcttag tggatgcttc 8041 tgaactgtca aattgtcctc caacaagtta catcatttta tacttccaac gttatgtgag 8101 agttcatttt tctttaggct cttctagtat tttatatttg agaaataaaa tttaaccaaa 8161 atttgccaat ctagtaagct tgtttttatt tgtatcttta aattttatga tagatgttat 8221 agtgtactct cctctcccct cccctcccct ctcctcctca gcaggatctt gttatgtagc 8281 cctaagggat ctaatactta ctctgtagtc caggctgtcc ttcagctcat gagacacacc 8341 atcatgcttg gcatgtttct tgttctgaaa ggtatctggt tttaattttg gttgagctgg 8401 tgtgttggtg tatatctgta atctcagcac aggcaagtag agacaaggat tatgattaca 8461 gtccagcctg ggctatttag agggattctc tttaatcttt cctcatgccc caaaatctta 8521 cctttggtat atgaaaaata gtctccactt ctgcaaaata ttgctttatg aagtaagaat 8581 taattccctt catagagaca aggaatgtgt cctgtaaaag tttaatgtgt aagaagtatt 8641 tgttataaaa gataaatatt cagaatcttc tttttaattc ctgattttat ttctatagga 8701 ctgaaagact tgctcgagat gtcatgaagg agatgggagg ccatcacatt gtggccctct 8761 gtgtgctcaa ggggggctat aagttctttg ctgacctgct ggattacatt aaagcactga 8821 atagaaatag tgatagatcc attcctatga ctgtagattt tatcagactg aagagctact 8881 gtgtaagtat aattaactta taattaaaaa aatagggcca ttctagtttt atctatattt 8941 tttttaaact tgtgcaaact atgctacata atcaacttac ttgattatgt aatatatata 9001 gtaatagttg gaattactat tattacatat ttatttttct gtgctggggt agaacccagg 9061 gaactaagta ctgtaataac cgagcttcat tcatcctagt catttttaat tttttttttt 9121 ttttttgttt tttcgagaca aggtttctct gtgtagccct ggctgtcctg gaactcactc 9181 tgtagaccag gctggcctcg aactcagaaa tctgcctgcc tctgcctccc agtgctgaga 9241 ttaatgacgt gcaccaccac tgcctggcat ttttttaaag ttttaatttg agaccctttc 9301 tcactaactt gctcacacta tcctcgaact cattacgtgg gctggtctag aaatttcagt 9361 caacttgcct cagcttctca agtagctggg attacaggca ggtgctacca cttctggcca 9421 tgatcattgt tgttgttgct attattatta ttattatttc actgatactt catatcacaa 9481 cacagaatag taactgtagt tcatgaccaa caaaaaagtt gtacagtttc tctgttgtct 9541 tattttgatc tggattttga tttgtttcat ggcactgggc aggctaatct caaactgcat 9601 ggctcaaata gtccttctgt cttaatctcc catgttctgg aagtataggt atcactacac 9661 ctagctgtat tttcatattt taaaaaaaac gcctaagaat tgctattgaa tgaggcggag 9721 agatggctca gcagtcacaa gtacttgtag aggactgggg tttggttact agcacctcaa 9781 agatggctta caattatctg tagctccaat tccagaggat ctgtgtggta aagctgcatg 9841 tatatgggca tgcaggtaaa actgctaaac atataaaaat agctcagtct taagagaatt 9901 ggtcgacttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 9961 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 10021 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 10081 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc 10141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 10201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 10261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 10321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 10381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 10441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 10501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 10561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 10621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 10681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 10741 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 10801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 10861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 10921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 10981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 11041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 11101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 11161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 11221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 11281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccaggt cgagtaggga 11341 taacagggta atggatcctc gttgggcacc tgcactcatg tacgcaggcc cacatataga 11401 tgtacacacg catatcattc aaaatagaaa ttatttatac aagtagtgcg ttggagagct 11461 ggctcaggag ttgttaatgc ttgctaccct tgcaaaggac ccaagttcaa tttccagcac 11521 ctgtgttggg catctcacaa ccacatgtaa atctagtttc aggggatttc acccctcttc 11581 tgaactcttc aggcacttat acccacaaat ggacatctcc acatagagac acatactttt 11641 aaaaaagtaa tctagggtct agagaaatgg ttatagacca cggactggga tcagttccca 11701 gaagccacat cctgtggctc acaattgcct gtgaatcaag ttctagatct ggtggcctcg 11761 tctagcctgc agtcatcaac aaacacatgc acacatccat acacacataa tttaaaagaa 11821 agcgaaagta acctataatg ctaaacaaag cacattctca gtgtagactg cagtggtgat 11881 gaaccttgaa aatatattaa tagaagccag acacgtaagt cacaaatggt ataactcaat 11941 ttatatgaaa ttcccagaga gtccaaagtt aaaaaaaaca aaaaggcaga ttattggttg 12001 tcagtagcta cagagatggg acattgaaga tattaatagg tatagggttg aggattcatg 12061 gcctttccct cttccatgtt agcatgtcta ttgttgttaa ccttattcaa ctcatgttag 12121 ggcaatcata ttgatgagac ttaatgagta tagcttgaca ttcctagaag acagagtctc 12181 acatcagact ctctgatcct ctgactctta gaatctttca acaccctctt ctgaaatgat 12241 ctctgagcct ttggttgtga ctgaattatg agaaagaatt ttatgttgaa tgaattttac 12301 ttcagcattt ttacaaaact gaagctgggg atggcgtttc atgcgtgcag tccctaccat 12361 ttgcctataa gcctggagga ggcagaagta gaattaggct tttcagggac atcttcagct 12421 gcatcatgag cttgaggaca acttgagcta cataagattc ccatccccac ccccacacac 12481 acacacacat ctagtatgta cctagtatgc agtcttttca tatgtttaaa atgtagaaag 12541 gtatatgtga agtctccctt ttccccacaa cttagtccct ccaaagtatt gcatcagttt 12601 ccttgtacat gattttatgc atatataaga tacactattt ctctctctct ctctctcctt 12661 ccctccttcc ctccaacctc tgggtatgag ttgagggtta tgtatgtttc aagttgtaat 12721 aagaattgcc actcaaacat ttcatcccat tttaagttaa gtttgtctga ttttccactt 12781 tagaagggca gccatgatgg ctcatgccta taatcccatc ctagcacttg taagcctaat 12841 acataagact ggaggattgt catcagcttg aggctagcct ggtctaccta atgagttcca 12901 gccagccaag agtagaatat aagaccctgt ctccaaaaaa tgtaccaaag gggctggagg 12961 ggtaactcag tggttaagaa cagtagctgc tcttccagag gacctgggtt caattcccag 13021 cacccacatg gcagcttgca actatctgta actgtccagc tttgggatat ccgacaccat 13081 tataccaatt acataaaatg aaaaaattgt aaaatgtgct aaaatgagtg gggaggggga 13141 gcatggtgtg gttctgtggg cttgggattt tgccatgaat ccatgactat atctcagcaa 13201 tatgtggatt atttctggaa acctgtgggg catgggtgta tgggaagagg ggagagagag 13261 agagcccgca tccagccaga gttcctgtgc tctgggcagg cggacgtggg aggactgctg 13321 gacgatttcc acttggccct gggtgggcat ctggctgtgt gaagccactg accccactca 13381 gcggggggta gataaggggc atttcccata ccaggttctc agactccagc attccacgct 13441 ggatacagca gaagagctga gaacagaata ggggccccag ggagacactc tcgtcccaga 13501 gataaagaag gcagagggag agagattcct acgcaggcga gaggccttag tctgggcctt 13561 gatgaacaga gacggtctat ggttttagag ctttattgta gaaaggcagg gggaaagaga 13621 gaaggtagaa agagagagac cagccatggc caagaggaga gaagggggga aaggagagag 13681 agaagaaagg ctagagagta agaaaggtta aagagacaga agtaaaagct tagagtaaga 13741 gagagagaga gagcaagagg gagagtaaga aaagtaaaag cttaagagag cgaggagggg 13801 ccagcccctc taatagtggg cttgttatct tgttgttgct aggtaactgt gaagcgtcta 13861 gcctgaaggc cagaagcttg ggacattgtc tatgtgactg aaagccatga gcctctactg 13921 tgggggctgt ggaggtggta acttggacag gagccagggg ttccaggaga catgagggaa 13981 tgccttctgt cccatgtagg taggaattac caccaccggc tttcaccgta gttcactgga 14041 gactgtttgt acatagccca ttgccccaca gaaaccaggc actcactgtt tcatttgcat 14101 aagaattaga aggctttatt ttggctggga ttggtgactt gccccagaaa ttccagcttt 14161 tgggaagccg aatcctggag aaccaggagt tcaaggtcat cctcaggtac ctcatttgtt 14221 caagaccagc ctgtgctacc atgagacctt gtccctactc ccctcaaccc caaaacacgc 14281 tgttttcatt ttataggttg tactaaccat ggaggatggt catttgcctt tctcttgatt 14341 tcagaagcgt agcgcgggct ttgcaccatg atctagtgtt tccagatagg cttgcctctc 14401 cacagccagg cttatcctgc ttcagtccca tgtttggcaa gggcaacata tgactgaaga 14461 acttgcatag ccttatggtg tatcatgact cttagcccca gtttataact gtctaacagc 14521 tggtggcttt tgtgaaaaag gaaagcatca tttgaacttt taacttttca gctgtttgtt 14581 ttgactttgc ataaatactg agactagcta ggaaatgagc ttttgtttca tttgctaaaa 14641 atgatttttt tttcatgagg caatttcaag caatttgaat ttagcaatct ctacttgtct 14701 gaaaagaagt tattaattaa aaaaaaacaa acaaacagga acctattatg ctggctagtc 14761 acatgaaaga atccaaatga acacagtcaa ggctgaatca acacatgaaa ttattttcta 14821 attccccaaa ttacatttat gctaattagt atgtagtaaa acattttaat tgtttccaat 14881 gttaaacatt acagaaataa ttttgatgtt ttcctaataa cccagccttt gatcatgttt 14941 gcatttattt gattgttgtc agtttattaa ataattaagt aagagcaaca agataaaatg 15001 acttcatgag agtccttttc tgtaacttag aacgtcagta gtcataggaa ctgcggtcgt 15061 tcccttacct ttcagaagta ggctaaagag ttgaacgcaa aggtggtttt tcgaatcttc 15121 ctgatcctta agcccccact aggtttatat tctatatttg atatcggatt cgaacccctc 15181 gagcttagga tccggaaccc ttaatataac taagttatta ggtccctcga agaggttcac 15241 tagtactggc cattgcggcc gcttcgaggc tgcctcgcgc gtttcggtga tgacggtgaa 15301 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 15361 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 15421 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga 15481 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 15541 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 15601 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 15661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 15721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 15781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 15841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 15901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 15961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 16021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 16081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 16141 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 16201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 16261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 16321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 16381 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 16441 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 16501 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 16561 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 16621 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 16681 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 16741 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 16801 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 16861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 16921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 16981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 17041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 17101 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 17161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 17221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 17281 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 17341 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 17401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 17461 gcacatttcc ccgaaaagtg ccacctgacg g //