LOCUS Ple162\(pEMS1358 20087 bp DNA circular 17-NOV-2008 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2455 - 5' - TGTTCTCCTCCAACCAAAAGGC - 3' Tm=56.9 Deg C Antisense primer: oEMS2446 - 5' - TAAAAGGGATCGGCTGGCAG - 3' Tm=56.6 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 401bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|499190435| COMMENT VNTDBDATE|499190435| COMMENT LSOWNER| COMMENT VNTNAME|Ple162 (pEMS1358)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#191=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -255 100) COMMENT (CObjectList) (CObjectList)) COMMENT #192=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -185 100) COMMENT (CObjectList) (CObjectList)) COMMENT #193=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -395 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #194=(AnlzFontBackColorItem 3109 3278 41976063) COMMENT #195=(AnlzFontBackColorItem 138 2897 33606707) COMMENT #196=(AnlzFontBackColorItem 2920 3108 33606707) COMMENT #197=(AnlzFontBackColorItem 2898 2919 43633766) COMMENT #198=(AnlzFontBackColorItem 3299 3776 41976063) COMMENT #199=(AnlzFontBackColorItem 3279 3298 43633766))) (CObList) 17498130 COMMENT (CStringList) 0 327734 (CObList))) FEATURES Location/Qualifiers misc_recomb 13950..17761 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker complement(18860..20081) /vntifkey="22" /label=AmpR misc_marker 12499..13924 /vntifkey="22" /label=AmpR misc_recomb 8858..12503 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4904..8857 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 13931..13948 /vntifkey="21" /label=I-Sce\I misc_feature 3786..3819 /vntifkey="21" /label=MFRT\(F5mutant) misc_feature 3820..3830 /vntifkey="21" /label=Kozak\consensus misc_feature 3827..4546 /vntifkey="21" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 4547..4582 /vntifkey="21" /label=Tetracysteine\residue misc_feature 4583..4655 /vntifkey="21" /label=NLS misc_feature 4661..4694 /vntifkey="21" /label=MFRT misc_feature 4848..4898 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 3110..3777 /vntifkey="21" /label=Prom\region misc_feature 139..3109 /vntifkey="21" /label=Region\2 misc_feature 139..3777 /vntifkey="21" /label=Ple162\(PITX3-D) primer 2899..2920 /vntifkey="27" /label=oEMS2455 primer 3280..3299 /vntifkey="27" /label=oEMS2446 BASE COUNT 5152 a 4760 c 4645 g 5530 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatgaatt 121 cgagctccta ggccggcccg attgtgggct ctcactatgt ccctggtcta cctctctggc 181 ttcaggactt aagtgggtcc acatgacagc tgacttattg ccatggggtt gtgtctcttg 241 gaaaccatgg cctccccact gtggcctccc tggcctggga tctgctctcc accttcatat 301 gtcctggata tttcattctt gctgcagtcc atgaccaggc ttcctgtaat tccagcctgt 361 actacagaat cagagcatac ctgtaggccc tctgtactcc ctgggcctgg gattgccacc 421 ctgataccag tgggactggg ccacacatcc ctcacatccc tagcagggtg tggcaaaaag 481 tctaggggtg gggccggcag cccccttcca gagctggact caccatcact gttatcttct 541 gactctctct gtccctgcct gggcatttct tactagcatc aaccaatcct gcggcttcca 601 gacctactgc ttgttcttcc ctatccactg agtgaggcga agtccctggc ctcagtccca 661 actccctagc ttcctgacct ccagcagttt gggggagctt aatcttcagc cagcctgtct 721 ccagctcaga caacagcttg gcgcccccag cctttgcctg accagcccac ccggacgtga 781 gtcggctaga ggtctatgcc cccacccttc catctctcta ttgcctgcct ggctttactg 841 ttctttcctt ctctcttcag tttcacggtc aggtcccttc tcagctgtgg ctcctctctc 901 tgctgttact agcccatgca agagcctaaa ggcttcattc tttcctctac cagtcccttg 961 tccccaaagt ggctcccagc ttgggttact ctgcccattt tgctgttacc ttgttgactg 1021 ggtcacctat gctcaggaca gagtgttcac agcatgtgtt gcctatctct cagtaatgca 1081 gcgtactcac cttcattcac cagggaaaca cttcactgga ggatacagag tctgggcaga 1141 tgactcactc aaggaagggc agacacaggg atgagatctt agccaccact ccaccctcag 1201 tgcctaactc aatgcccagc tcatagaatg tgctaaataa acatttgttt aatgagtgag 1261 gaaaaaaaac tactcaggta gagttttatc cctactcata tttacatgtt tatgttttta 1321 tctaaacctt ataacaacac tgaaggcaag tagggaaggg atcatgaact tcctttcgga 1381 gatgaagaaa tgtgctcaga gaggtaaagt aacttgccga ggacacacag ctagtaagtt 1441 gtacagccag gacgtgaatc tagatctgct gactctaaac ctaatttctt tgggctatac 1501 cagatgactt tcctgagagg ggcctgggca ctttctgccc ctcctcccca ccccatcctc 1561 tagctccagc attctcatgc cttctcctca ctcagcagca tccagtgcag aacactcccc 1621 tggccccttg gcctgctcct cccccacaca gagtccttag ctcctgggac tgagagctga 1681 ggttcagagg ggccagggag gcggtggggg ggggggggtg ggtgggggaa gggtaataat 1741 ggctagctcc aaaacagccc cggcagctgt ccctgtcaca gagaggagac tgtgtgacgg 1801 tacgtgtctg tctgcctgga tgtggcagcg catgtgtggg agagtgtgtg tttgtgtgcc 1861 cctagctcca agtccaagtg cttattatgt ctgagtgggg gcctgtgtgt gtctgcacat 1921 gtgtctgtct gtctctggga acacgcagcc tgaactcggc tttctggcct cggcttttcc 1981 tcgcccatat cccttctcac cctgcctccc tctgctgaaa taaagtgaaa gcaagatgca 2041 ggtatggaat gccggggaca gggtaatttg gaaaactgca agtcgggagc caaagctgac 2101 tggaaggcta ggtggcaagg gtgggactgc ttttcccaca ggtaaacccc tctctgggtt 2161 ttttcccagc tccatgttgc ttccatggcc tctggaaaag tcagggggat gatttgcatt 2221 tcaagggcat cttgggtatg aagtaagggg tatgaagtaa ggggagactt cagagcaccc 2281 ctttttccaa ctctcagttc taacccttcc tccctcatct tcccagccaa ggctaccata 2341 gttccttctc agggacatct cccttccatc agagcattgt tggccaaagt tttccagctc 2401 caaaccccct gacctgaccc gacctggaag gggaagtaag ctgagcacac ctccagttct 2461 ggcctgactt ttctcctgtc ctgggttccc tccagccctg tctcttcaca cccaataaaa 2521 ggtccaagat ctagacccct ctgcctgtgg aaagagcagg gactctttct caacctcctg 2581 ccttgacctc ggttccactc ccaacctggc ttgagaggtg gggtggggag tgggggcaca 2641 gctgtgatct ctgagctgtt gaagggggca ttaccatgtg caaatacagg ctgctgccca 2701 tctgcacctc cctgagaaag gctcctaata aatccagagc agccaagtga cacacatctc 2761 aatctaggct aatccaccac attaatgcca tcctgtgcag cctgcattaa gcccctgtta 2821 atggggaagg ggtgagagag gcagagaggc ccctcccact cagctctcta ccccttccct 2881 ctgtccctag cctccttctg ttctcctcca accaaaaggc acccagagtc ttgagcccca 2941 gtattgccca tttcccttcc tcccagcaga ggggtcctgg gagagcaggc agaaaagggg 3001 accctgcggg ggaaacttgg agaaggaccc tgggaggctc ctgggcaatg tgaggctaag 3061 tttgggaaag gaagtatgag taggaaggtg gtctaatgaa gctcctgccg ggaggattgg 3121 aacagagaca aaagggagga gagacggaca gcgacaagtg gagaaaatcg gcgaaacttg 3181 agtggcagag aagtctgagc gctgagaccc ggcggccccg tgcgccttcc cacctggcgc 3241 cgatccactt tcctcggggt agcggcccaa cccacttcgc tgccagccga tcccttttac 3301 ccgtggctac cgggaccact ctactctcgc ccacttggct ctgcctaagc gtcctagccg 3361 gagcgcggtc tctgccacgt ggggaggggc gcggccgagt tgctgaagag cgcttctgat 3421 tggccagagg gcggggttct tggcgtctcg ccggccagac ccctccctca aaggcggggc 3481 ctggagatcc acagctggaa agggcggagc cccagcaggg cagctggaaa ggggcggggc 3541 ctgacgcgcg cggctcgccg cggcgggctg ggggcgccct ggtctgccat aaagtgaatg 3601 ggcgccggct gggggtggca gtacgcggtg aggctcactc cctccgagag tccaggagcg 3661 cccgagcgga gaggcggccc gggagcaggg gggcggcccc cactccggcc gggtgcccgg 3721 cccctggccc ctgcctgccc tctagatcgc cgccgcagcc gccgctactg ggagtctggc 3781 gcgccgaagt tcctattctt caaaaggtat aggaacttcc gccaccatgg tgagcaaggg 3841 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 3901 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 3961 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 4021 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 4081 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 4141 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 4201 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 4261 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 4321 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 4381 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 4441 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 4501 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagttatttc tgaactgctg 4561 cccgggctgc tgcatggaac cggatccaaa aaagaagaga aaggtagatc caaaaaagaa 4621 gagaaaggta gatccaaaaa agaagagaaa ggtaatataa gaagttccta ttctctagaa 4681 agtataggaa cttcagcggc cgcgactcta gatcataatc agccatacca catttgtaga 4741 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 4801 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 4861 catcacaaat ttcacaaata aagcattttt ttcactgcga attctcgtaa aactcttcat 4921 ggcagtagtt attattctct ctctctctct ttttcttttt tcttgagaca ggatatttct 4981 ctgttgccca ggctggagtg cagtggcaca gtcttggctc actgcagcct ggacctcctg 5041 ggctcaagcc atcctcccac ctcagcctcc caagtagctg gggctacagg cacatggcca 5101 ccaggcccag ataatttttc atttttgtag agactgagtc tcaccatgtt acccaggttt 5161 attattctca ttttttagat gaagagactg aggtccagag aagctcaatg acttgcctag 5221 ttttacaaat ctcctgccat cacatacccc tcagcgtcct taataagagg gaggccacca 5281 actatgtgct gggcactgtg gtggatgctg gagctatagg gttgagtata taagaaatgg 5341 tgttgctgga gcaactgttg cttgcttacc tgacctatct gagaattaat tagcagggga 5401 acatattttt gttttcagat tcaatataag aacttgtgtg ggcaaaaata aagatcagta 5461 gtaataacag tagttcccat ttgctgactg tactgtccta agtgcatata tatatacata 5521 cacacacgca tacctatact cctctaatac tcaaaatgat cctgtttatg tattgttaat 5581 atgctcattt tatttttaaa tttttattta tttttatttt tatttatttt tgagacggag 5641 tctcattctg tcgcggaggc tgaagtgcag tggtgcgatc tcagctcagt gcgacctccg 5701 cctcccgggt tcaagtgatt ctcctgcctc agcctccgga ctagctggga ttacaggcgc 5761 ccgcctccac gcccagctaa tttttgtatt tttagtagag atggggtttc gccatgttgg 5821 ccaggctggt ctcgtactcc tgaccttgag tgatccacct gcctcggcct cccaaagtgc 5881 tgggattaca ggcatgagcc accgcgccgg gctaatatgc tcattttagt gaggcaaaaa 5941 tagaggctca gagtctgatt tgtacaaaac tacagagcag ttaagtgtcc tctcagatgt 6001 gtaccctgat ctgggtgact ctaggactct aggtctcaac tgttacaacc agttaagggt 6061 ttggggaagc actgggccaa gagtcaggaa aatggaagcc acaggtagtg caaggtcttg 6121 ggaatgggac gtctggtcca aggattcacg cgatgactgg aacccgaaga gccggggccc 6181 ggtttacggc cgccatgaag caacgcgcgc cggtaggttt gggaatcagg gagccctctg 6241 aataggagac tgagttggga gggaaagggg cttcgctggg ggagcctcgg cttcttctgg 6301 gagaaaattc ccacggctac ctagtgagcc tgcaaactgg taggcgccgg cgtaggcgcg 6361 cgggcggggc cgggggcggg gcctgcgggg cgtggcgggg cgggcagagg gcggggcctg 6421 cttctcctca gcttcaggcg gctgcgacga gccctcaggc gaacctctcg gctttcccgc 6481 gcggcgccgc ctcttgctgc gcctccgcct cctcctctgc tccgccaccg gcttcctcct 6541 cctgagcagt cagcccgcgc gccggccggc tccgttatgg cgacccgcag ccctggcgtc 6601 gtggtgagca gctcggcctg ccggccctgg ccggttcagg cccacgcggc aggtggcggc 6661 cgggccctga ggcgcgggat cgatccatga cacccccccg ccccccatat ctcactataa 6721 ttcactgtca aatggctttt atgatttgga gtttggggat acttttaaag acagggtctt 6781 ggtagctcct agatggcttg ggattcactt agtaaccctt gctggcctaa aattcaggaa 6841 cttctacctc atcttcccaa gtactgggtt agtagggatg tccagcatat ttagcttgct 6901 ttaatgattt tgctgtactt ttttctgtgt gtacaggtgc atgtatattt ataaaactaa 6961 cataatatat tttaccccca aagtaccata accctaatta atgctattaa atgttttgta 7021 tgctgggctc tgggcatact aatataacca atattataca atgcaaaaca attttggaat 7081 atcttctgta aaattatctt attagtaagt tgtaaccatc tcagacttct aagtttgagt 7141 ttgtaaagga tcagtttttt ttggtgacct acacacacac acacacacac acacacacac 7201 acacacacag gggggtggat tctgagaatt tagagaattt agatggttaa taccagtttt 7261 tatttttatt ctgtgtgata tttatttatt aagatttatt tatttattac atgtaggtac 7321 actgtatctg tcttcagaca caccagaaga gggcatcaga tctcgttaca gatggttgtg 7381 agtcaccatg tggttgctgg gatttgaact caggacctct ggaagaacag tcagtgctct 7441 taaccgttga gccatctcac cagccccgtg atattttatt taacttacta gaatatactg 7501 tgttaaaaca tattttaggg gattggggag atggcccatt ctgtaaagtg tctgccatgt 7561 catcttcagg acctgcgttt gtaaccccag catggagaag acagagacag ggagacccct 7621 gggcttcctg gcaagctaga ctagcaaatt tggttaacta ggttcaatga gagactcagt 7681 ctcaaaacat aagaagtgaa ttttttagac ccaatttcaa tctctgaact ccatacgtat 7741 gcacatgtac ctgcatataa atatatatac acacccataa acacatgcac acacaaaaaa 7801 tggttcaaaa gttcttaaaa tgtataagag tagaaagtgt agagccggga gtggtggcgc 7861 acgcctttaa tcccagcact tgggagcaga ggcaggtgga tttctgagtt cgaggccagc 7921 ctggtctaca gagtgagttc caggacagcc agagctatac agagaaatcc tgcctcaaaa 7981 aaccaaaaat aaagtgtata tactaatctt tcatgcctaa aatatttatt tttgccacat 8041 ttttcatacc tctttaatat ttttcttttt ctctttgtgc ttcaaaggaa attttgtgtc 8101 atgttttact tttcattctt gactctattc agcagtaaga cgcagcatgg ctttcttaga 8161 gaagtataat ctattacaaa acggtaacat ttaactgctc tacataccct cttgtagcta 8221 aattcctctg ttaaactaag acttattatc taaacgtaat taaatgttga tatcttataa 8281 tgctccactt tgaaacagct gtctttcttt tgcagattag cgatgatgaa ccaggttatg 8341 acctagattt gttttgtata cctaatcatt atgccgagga tttggaaaaa gtgtttattc 8401 ctcatggact gattatggac aggttagtaa gacctcgatt gaagttattt ttagagggtg 8461 tgtgtgtggt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtaa gagagagagg 8521 agaggcatgt gtgatatata tgtgaagtgt attgcatgta catggaatcc agagaaggac 8581 attaggtgtc ctactctgtt gctctcttat tcccttgaat cagtgtctct tgctgaacct 8641 ggggcataca tactgatttg gctaggctgc ctggctagca agacccaggg atctgcttgt 8701 ctctgccccc cagtgctgac gttacaagca ggtgcaacca catctggttt ttatatgggt 8761 actgggggat ctgaacttag ggcctgatgc gtgcccaagt gctctcaccc gccaagccac 8821 ctctccagcc aaagggtttt ttttcttgta ttaattctaa cttagcaact gttgagtagt 8881 tgatgtttga atataagcta agccaactta ttagaataaa tgttttctat tttttacttg 8941 tatgtgtgag tgcctgcatg tatgtatgtg aagcatatgt attccaggat gtcagaattg 9001 tgatggattc cctggaactg gagttacaga ccattgtgaa ccactgtgta tgggtgctgg 9061 gaactgaact ttaggtcctc tgcaaaaaca gtaaggatct taactgataa actatctttc 9121 tagccctaag gctttctact tttaaagttc gtttctcttt ctctctctct ctctctctct 9181 ctctctctct ctccctctcc ctccctcctc ctcctcctcc tctttccccc ctctcgcctc 9241 ctctttctct ctcccctccc tctctctctc tcacacacac acacagacag agagaggaga 9301 gagaaaggaa gccttactaa ttaattacct agttttagac ataactagtg ggaatccata 9361 gtaagtaaat caacattctt gaagagaggt tagaagaatt ggctctcgtt gtccatcgag 9421 gctgtataag attattcttc tgttttctca gcttaccata catattttct acgtagacaa 9481 taccaattca aatacactgt acgtaaagta tatgcgcaca cacacacaca cacacacaca 9541 cacacacaca caagttacat attgtcatat agtactaaaa tttatggact ttttcatatt 9601 aatacatcta gagctatcat gttttttatc ttttaaaaat tactttacgg ggctacagag 9661 atgtctcagc agttaggagc actgactgct cttccagagg tcctgagttc aattcccagc 9721 aaccacatgg tggctcacaa ccatctgtaa tgggatctga tccactctta tggtgtgtct 9781 gaagacagtg acagtgtact catatacatg aaataaatct ttaaaaaatt actttaccat 9841 cagggtatat atatatatat atatgtgtgt gtgtgtgtgt gtgtgtatga agtatttgct 9901 atttgagagg gtctcactgt ttagctcagt ctggcttgga acgttctgta tagcccagac 9961 tggcctcaac tcagcagtcc ccctgaaagg ctctcatatg tggagattac aggtatgtgc 10021 tactgtgccc agccctacat gttactttgt ttttgaaata gggtatcaaa tagcttagac 10081 tggcttctaa gtcagtgtgt agctaaggat ggtctgagct tctgaacctg ctacttcaac 10141 tcctggtgtg ccatcatggc cagtttgcac agttgtgaga gtagaactca ggacctccta 10201 tgtgttacta gacatgttct ctaccagctg agctacatcc caattcgcct acatttactt 10261 ttatttttta aaagatttat ttatgtatac agcactctgc ctgcatgtac atctgcatgc 10321 cagaagaggg caccagatct catcatacat agttgtgagc caccatgtgg ttgctgggga 10381 ttgaactcag ggcctctgga agagcagtca gtgctcttaa cctctgagcc atctctgtag 10441 ccccctacat ttattttaaa gattataggc tattctattc atagatgtgc cataactgat 10501 ttaaccagat cttcattatt ttgtacttag tagagtatat tagagggaat gaatcttaaa 10561 tacacatttt gacacttgct cagctttaga caagtaaaaa tcactggtca aggaaatggt 10621 gcttagtgga tgcttctgaa ctgtcaaatt gtcctccaac aagttacatc attttatact 10681 tccaacgtta tgtgagagtt catttttctt taggctcttc tagtatttta tatttgagaa 10741 ataaaattta accaaaattt gccaatctag taagcttgtt tttatttgta tctttaaatt 10801 ttatgataga tgttatagtg tactctcctc tcccctcccc tcccctctcc tcctcagcag 10861 gatcttgtta tgtagcccta agggatctaa tacttactct gtagtccagg ctgtccttca 10921 gctcatgaga cacaccatca tgcttggcat gtttcttgtt ctgaaaggta tctggtttta 10981 attttggttg agctggtgtg ttggtgtata tctgtaatct cagcacaggc aagtagagac 11041 aaggattatg attacagtcc agcctgggct atttagaggg attctcttta atctttcctc 11101 atgccccaaa atcttacctt tggtatatga aaaatagtct ccacttctgc aaaatattgc 11161 tttatgaagt aagaattaat tcccttcata gagacaagga atgtgtcctg taaaagttta 11221 atgtgtaaga agtatttgtt ataaaagata aatattcaga atcttctttt taattcctga 11281 ttttatttct ataggactga aagacttgct cgagatgtca tgaaggagat gggaggccat 11341 cacattgtgg ccctctgtgt gctcaagggg ggctataagt tctttgctga cctgctggat 11401 tacattaaag cactgaatag aaatagtgat agatccattc ctatgactgt agattttatc 11461 agactgaaga gctactgtgt aagtataatt aacttataat taaaaaaata gggccattct 11521 agttttatct atattttttt taaacttgtg caaactatgc tacataatca acttacttga 11581 ttatgtaata tatatagtaa tagttggaat tactattatt acatatttat ttttctgtgc 11641 tggggtagaa cccagggaac taagtactgt aataaccgag cttcattcat cctagtcatt 11701 tttaattttt tttttttttt ttgttttttc gagacaaggt ttctctgtgt agccctggct 11761 gtcctggaac tcactctgta gaccaggctg gcctcgaact cagaaatctg cctgcctctg 11821 cctcccagtg ctgagattaa tgacgtgcac caccactgcc tggcattttt ttaaagtttt 11881 aatttgagac cctttctcac taacttgctc acactatcct cgaactcatt acgtgggctg 11941 gtctagaaat ttcagtcaac ttgcctcagc ttctcaagta gctgggatta caggcaggtg 12001 ctaccacttc tggccatgat cattgttgtt gttgctatta ttattattat tatttcactg 12061 atacttcata tcacaacaca gaatagtaac tgtagttcat gaccaacaaa aaagttgtac 12121 agtttctctg ttgtcttatt ttgatctgga ttttgatttg tttcatggca ctgggcaggc 12181 taatctcaaa ctgcatggct caaatagtcc ttctgtctta atctcccatg ttctggaagt 12241 ataggtatca ctacacctag ctgtattttc atattttaaa aaaaacgcct aagaattgct 12301 attgaatgag gcggagagat ggctcagcag tcacaagtac ttgtagagga ctggggtttg 12361 gttactagca cctcaaagat ggcttacaat tatctgtagc tccaattcca gaggatctgt 12421 gtggtaaagc tgcatgtata tgggcatgca ggtaaaactg ctaaacatat aaaaatagct 12481 cagtcttaag agaattggtc gactttttcg ccctttgacg ttggagtcca cgttctttaa 12541 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 12601 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 12661 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga 12721 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 12781 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 12841 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 12901 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 12961 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 13021 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 13081 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 13141 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 13201 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 13261 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 13321 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 13381 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 13441 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 13501 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 13561 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 13621 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 13681 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 13741 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 13801 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 13861 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 13921 ccaggtcgag tagggataac agggtaatgg atcctcgttg ggcacctgca ctcatgtacg 13981 caggcccaca tatagatgta cacacgcata tcattcaaaa tagaaattat ttatacaagt 14041 agtgcgttgg agagctggct caggagttgt taatgcttgc tacccttgca aaggacccaa 14101 gttcaatttc cagcacctgt gttgggcatc tcacaaccac atgtaaatct agtttcaggg 14161 gatttcaccc ctcttctgaa ctcttcaggc acttataccc acaaatggac atctccacat 14221 agagacacat acttttaaaa aagtaatcta gggtctagag aaatggttat agaccacgga 14281 ctgggatcag ttcccagaag ccacatcctg tggctcacaa ttgcctgtga atcaagttct 14341 agatctggtg gcctcgtcta gcctgcagtc atcaacaaac acatgcacac atccatacac 14401 acataattta aaagaaagcg aaagtaacct ataatgctaa acaaagcaca ttctcagtgt 14461 agactgcagt ggtgatgaac cttgaaaata tattaataga agccagacac gtaagtcaca 14521 aatggtataa ctcaatttat atgaaattcc cagagagtcc aaagttaaaa aaaacaaaaa 14581 ggcagattat tggttgtcag tagctacaga gatgggacat tgaagatatt aataggtata 14641 gggttgagga ttcatggcct ttccctcttc catgttagca tgtctattgt tgttaacctt 14701 attcaactca tgttagggca atcatattga tgagacttaa tgagtatagc ttgacattcc 14761 tagaagacag agtctcacat cagactctct gatcctctga ctcttagaat ctttcaacac 14821 cctcttctga aatgatctct gagcctttgg ttgtgactga attatgagaa agaattttat 14881 gttgaatgaa ttttacttca gcatttttac aaaactgaag ctggggatgg cgtttcatgc 14941 gtgcagtccc taccatttgc ctataagcct ggaggaggca gaagtagaat taggcttttc 15001 agggacatct tcagctgcat catgagcttg aggacaactt gagctacata agattcccat 15061 ccccaccccc acacacacac acacatctag tatgtaccta gtatgcagtc ttttcatatg 15121 tttaaaatgt agaaaggtat atgtgaagtc tcccttttcc ccacaactta gtccctccaa 15181 agtattgcat cagtttcctt gtacatgatt ttatgcatat ataagataca ctatttctct 15241 ctctctctct ctccttccct ccttccctcc aacctctggg tatgagttga gggttatgta 15301 tgtttcaagt tgtaataaga attgccactc aaacatttca tcccatttta agttaagttt 15361 gtctgatttt ccactttaga agggcagcca tgatggctca tgcctataat cccatcctag 15421 cacttgtaag cctaatacat aagactggag gattgtcatc agcttgaggc tagcctggtc 15481 tacctaatga gttccagcca gccaagagta gaatataaga ccctgtctcc aaaaaatgta 15541 ccaaaggggc tggaggggta actcagtggt taagaacagt agctgctctt ccagaggacc 15601 tgggttcaat tcccagcacc cacatggcag cttgcaacta tctgtaactg tccagctttg 15661 ggatatccga caccattata ccaattacat aaaatgaaaa aattgtaaaa tgtgctaaaa 15721 tgagtgggga gggggagcat ggtgtggttc tgtgggcttg ggattttgcc atgaatccat 15781 gactatatct cagcaatatg tggattattt ctggaaacct gtggggcatg ggtgtatggg 15841 aagaggggag agagagagag cccgcatcca gccagagttc ctgtgctctg ggcaggcgga 15901 cgtgggagga ctgctggacg atttccactt ggccctgggt gggcatctgg ctgtgtgaag 15961 ccactgaccc cactcagcgg ggggtagata aggggcattt cccataccag gttctcagac 16021 tccagcattc cacgctggat acagcagaag agctgagaac agaatagggg ccccagggag 16081 acactctcgt cccagagata aagaaggcag agggagagag attcctacgc aggcgagagg 16141 ccttagtctg ggccttgatg aacagagacg gtctatggtt ttagagcttt attgtagaaa 16201 ggcaggggga aagagagaag gtagaaagag agagaccagc catggccaag aggagagaag 16261 gggggaaagg agagagagaa gaaaggctag agagtaagaa aggttaaaga gacagaagta 16321 aaagcttaga gtaagagaga gagagagagc aagagggaga gtaagaaaag taaaagctta 16381 agagagcgag gaggggccag cccctctaat agtgggcttg ttatcttgtt gttgctaggt 16441 aactgtgaag cgtctagcct gaaggccaga agcttgggac attgtctatg tgactgaaag 16501 ccatgagcct ctactgtggg ggctgtggag gtggtaactt ggacaggagc caggggttcc 16561 aggagacatg agggaatgcc ttctgtccca tgtaggtagg aattaccacc accggctttc 16621 accgtagttc actggagact gtttgtacat agcccattgc cccacagaaa ccaggcactc 16681 actgtttcat ttgcataaga attagaaggc tttattttgg ctgggattgg tgacttgccc 16741 cagaaattcc agcttttggg aagccgaatc ctggagaacc aggagttcaa ggtcatcctc 16801 aggtacctca tttgttcaag accagcctgt gctaccatga gaccttgtcc ctactcccct 16861 caaccccaaa acacgctgtt ttcattttat aggttgtact aaccatggag gatggtcatt 16921 tgcctttctc ttgatttcag aagcgtagcg cgggctttgc accatgatct agtgtttcca 16981 gataggcttg cctctccaca gccaggctta tcctgcttca gtcccatgtt tggcaagggc 17041 aacatatgac tgaagaactt gcatagcctt atggtgtatc atgactctta gccccagttt 17101 ataactgtct aacagctggt ggcttttgtg aaaaaggaaa gcatcatttg aacttttaac 17161 ttttcagctg tttgttttga ctttgcataa atactgagac tagctaggaa atgagctttt 17221 gtttcatttg ctaaaaatga tttttttttc atgaggcaat ttcaagcaat ttgaatttag 17281 caatctctac ttgtctgaaa agaagttatt aattaaaaaa aaacaaacaa acaggaacct 17341 attatgctgg ctagtcacat gaaagaatcc aaatgaacac agtcaaggct gaatcaacac 17401 atgaaattat tttctaattc cccaaattac atttatgcta attagtatgt agtaaaacat 17461 tttaattgtt tccaatgtta aacattacag aaataatttt gatgttttcc taataaccca 17521 gcctttgatc atgtttgcat ttatttgatt gttgtcagtt tattaaataa ttaagtaaga 17581 gcaacaagat aaaatgactt catgagagtc cttttctgta acttagaacg tcagtagtca 17641 taggaactgc ggtcgttccc ttacctttca gaagtaggct aaagagttga acgcaaaggt 17701 ggtttttcga atcttcctga tccttaagcc cccactaggt ttatattcta tatttgatat 17761 cggattcgaa cccctcgagc ttaggatccg gaacccttaa tataactaag ttattaggtc 17821 cctcgaagag gttcactagt actggccatt gcggccgctt cgaggctgcc tcgcgcgttt 17881 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 17941 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 18001 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat 18061 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 18121 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 18181 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 18241 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 18301 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 18361 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 18421 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 18481 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 18541 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 18601 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 18661 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 18721 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 18781 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 18841 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 18901 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 18961 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 19021 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 19081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 19141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 19201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 19261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 19321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 19381 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt 19441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 19501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 19561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 19621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 19681 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact 19741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 19801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 19861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 19921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 19981 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 20041 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgg //