LOCUS Ple162\(pEMS1148 21114 bp DNA circular 28-JUL-2008 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2455 - 5' - TGTTCTCCTCCAACCAAAAGGC - 3' Tm= Deg C Antisense primer: oEMS2446 - 5' - TAAAAGGGATCGGCTGGCAG - 3' Tm= Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 401bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|489082508| COMMENT VNTDBDATE|489082869| COMMENT LSOWNER| COMMENT VNTNAME|Ple162 (pEMS1148)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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1914717249 (CObList))) FEATURES Location/Qualifiers misc_feature 18800..137 /vntifkey="21" /label=pBS246\vector misc_recomb 14976..18787 /vntifkey="86" /label=5'\junction /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker 20107..21107 /vntifkey="22" /label=AmpR misc_marker 13504..14950 /vntifkey="22" /label=AmpR misc_feature 3803..3836 /vntifkey="21" /label=FRT\(F5\mutant) misc_feature 3837..3848 /vntifkey="21" /label=Kozak\consensus misc_feature 3849..4565 /vntifkey="21" /label=EGFP\(+mutated\stop) misc_feature 4566..5591 /vntifkey="21" /label=Cre misc_feature 5592..5664 /vntifkey="21" /label=NLS misc_feature 5668..5701 /vntifkey="21" /label=FRT misc_feature 5855..5905 /vntifkey="21" /label=SV40\early\polyA\signal misc_feature 160..3794 /vntifkey="21" /label=PITX3-D misc_feature 3127..3794 /vntifkey="21" /label=Prom\region primer 3297..3316 /vntifkey="27" /label=oEMS2446 primer 2916..2937 /vntifkey="27" /label=oEMS2455 /note="56.9" misc_feature 160..3126 /vntifkey="21" /label=Region\2 misc_feature 7687..13508 /vntifkey="21" /label=3'\arm\of\Hprt\homology misc_feature 5911..7686 /vntifkey="21" /label=HPRT\complementary\sequence misc_marker 14957..14974 /vntifkey="22" /label=I-SceI\cut\site BASE COUNT 5417 a 4993 c 4921 g 5782 t 1 others ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatataac 121 ttcgtataat gtatgcgaat tcgagctcct aggccggccc gattgtgggc tctcactatg 181 tccctggtct acctctctgg cttcaggact taagtgggtc cacatgacag ctgacttatt 241 gccatggggt tgtgtctctt ggaaaccatg gcctccccac tgtggcctcc ctggcctggg 301 atctgctctc caccttcata tgtcctggat atttcattct tgctgcagtc catgaccagg 361 cttcctgtaa ttccagcctg tactacagaa tcagagcata cctgtaggcc ctctgtactc 421 cctgggcctg ggattgccac cctgatacca gtgggactgg gccacacatc cctcacatcc 481 ctagcagggt gtggcaaaaa gtctaggggt ggggccggca gcccccttcc agagctggac 541 tcaccatcac tgttatcttc tgactctctc tgtccctgcc tgggcatttc ttactagcat 601 caaccaatcc tgcggcttcc agacctactg cttgttcttc cctatccact gagtgaggcg 661 aagtccctgg cctcagtccc aactccctag cttcctgacc tccagcagtt tgggggagct 721 taatcttcag ccagcctgtc tccagctcag acaacagctt ggcgccccca gcctttgcct 781 gaccagccca cccggacgtg agtcggctag aggtctatgc ccccaccctt ccatctctct 841 attgcctgcc tggctttact gttctttcct tctctcttca gtttcacggt caggtccctt 901 ctcagctgtg gctcctctct ctgctgttac tagcccatgc aagagcctaa aggcttcatt 961 ctttcctcta ccagtccctt gtccccaaag tggctcccag cttgggttac tctgcccatt 1021 ttgctgttac cttgttgact gggtcaccta tgctcaggac agagtgttca cagcatgtgt 1081 tgcctatctc tcagtaatgc agcgtactca ccttcattca ccagggaaac acttcactgg 1141 aggatacaga gtctgggcag atgactcact caaggaaggg cagacacagg gatgagatct 1201 tagccaccac tccaccctca gtgcctaact caatgcccag ctcatagaat gtgctaaata 1261 aacatttgtt taatgagtga ggaaaaaaaa ctactcaggt agagttttat ccctactcat 1321 atttacatgt ttatgttttt atctaaacct tataacaaca ctgaaggcaa gtagggaagg 1381 gatcatgaac ttcctttcgg agatgaagaa atgtgctcag agaggtaaag taacttgccg 1441 aggacacaca gctagtaagt tgtacagcca ggacgtgaat ctagatctgc tgactctaaa 1501 cctaatttct ttgggctata ccagatgact ttcctgagag gggcctgggc actttctgcc 1561 cctcctcccc accccatcct ctagctccag cattctcatg ccttctcctc actcagcagc 1621 atccagtgca gaacactccc ctggcccctt ggcctgctcc tcccccacac agagtcctta 1681 gctcctggga ctgagagctg aggttcagag gggccaggga ggcggtgggg gggggggggt 1741 gggggaaggg taataatggc tagctccaaa acagccccgg cagctgtccc tgtcacagag 1801 aggagactgt gtgacggtac gtgtctgtct gcctggatgt ggcagcgcat gtgtgggaga 1861 gtgtgtgttt gtgtgcccct agctccaagt ccaagtgctt attatgtctg agtgggggcc 1921 tgtgtgtgtc tgcacatgtg tctgtctgtc tctgggaaca cgcagcctga actcggcttt 1981 ctggcctcgg cttttcctcg cccatatccc ttctcaccct gcctccctct gctgaaataa 2041 agtgaaagca agatgcaggt atggaatgcc ggggacaggg taatttggaa aactgcaagt 2101 cgggagccaa agctgactgg aaggctaggt ggcaagggtg ggactgcttt tcccacaggt 2161 aaacccctct ctgggttttt tcccagctcc atgttgcttc catggcctct ggaaaagtca 2221 gggggatgat ttgcatttca agggcatctt gggtatgaag taaggggtat gaagtaaggg 2281 gagacttcag agcacccctt tttccaactc tcagttctaa cccttcctcc ctcatcttcc 2341 cagccaaggc taccatagtt ccttctcagg gacatctccc ttccatcaga gcattgttgg 2401 ccaaagtttt ccagctccaa accccctgac ctgacccgac ctggaagggg aagtaagctg 2461 agcacacctc cagttctggc ctgacttttc tcctgtcctg ggttccctcc agccctgtct 2521 cttcacaccc aataaaaggt ccaagatcta gacccctctg cctgtggaaa gagcagggac 2581 tctttctcaa cctcctgcct tgacctcggt tccactccca acctggcttg agaggtgggg 2641 tggggagtgg gggcacagct gtgatctctg agctgttgaa gggggcatta ccatgtgcaa 2701 atacaggctg ctgcccatct gcacctccct gagaaaggct cctaataaat ccagagcagc 2761 caagtgacac acatctcaat ctaggctaat ccaccacatt aatgccatcc tgtgcagcct 2821 gcattaagcc cctgttaatg gggaaggggt gagagaggca gagaggcccc tcccactcag 2881 ctctctaccc cttccctctg tccctagcct ccttctgttc tcctccaacc aaaaggcacc 2941 cagagtcttg agccccagta ttgcccattt cccttcctcc cagcagaggg gtcctgggag 3001 agcaggcaga aaaggggacc ctgcggggga aacttggaga aggaccctgg gaggctcctg 3061 ggcaatgtga ggctaagttt gggaaaggaa gtatgagtag gaaggtggtc taatgaagct 3121 cctgccggga ggattggaac agagacaaaa gggaggagag acggacagcg acaagtggag 3181 aaaatcggcg aaacttgagt ggcagagaag tctgagcgct gagacccggc ggccccgtgc 3241 gccttcccac ctggcgccga tccactttcc tcggggtagc ggcccaaccc acttcgctgc 3301 cagccgatcc cttttacccg tggctaccgg gaccactcta ctctcgccca cttggctctg 3361 cctaagcgtc ctagccggag cgcggtctct gccacgtggg gaggggcgcg gccgagttgc 3421 tgaagagcgc ttctgattgg ccagagggcg gggttcttgg cgtctcgccg gccagacccc 3481 tccctcaaag gcggggcctg gagatccaca gctggaaagg gcggagcccc agcagggcag 3541 ctggaaaggg gcggggcctg acgcgcgcgg ctcgccgcgg cgggctgggg gcgccctggt 3601 ctgccataaa gtgaatgggc gccggctggg ggtggcagta cgcggtgagg ctcactccct 3661 ccgagagtcc aggagcgccc gagcggagag gcggcccggg agcagggggg cggcccccac 3721 tccggccggg tgcccggccc ctggcccctg cctgccctct agatcgccgc cgcagccgcc 3781 gctactggga gtctggcgcg ccgaagttcc tattcttcaa aaggtatagg aacttcgccg 3841 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 3901 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 3961 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 4021 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 4081 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 4141 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 4201 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 4261 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 4321 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 4381 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 4441 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 4501 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 4561 agttatccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc gatgcaacga 4621 gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg ttttctgagc 4681 atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc aagttgaata 4741 accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta tatcttcagg 4801 cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac atgcttcatc 4861 gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt atgcggcgga 4921 tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg ttcgaacgca 4981 ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag gatatacgta 5041 atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa attgccagga 5101 tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat attggcagaa 5161 cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg gtaactaaac 5221 tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac tacctgtttt 5281 gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta tcaactcgcg 5341 ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag gatgactctg 5401 gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg cgagatatgg 5461 cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc aatgtaaata 5521 ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg cgcctgctgg 5581 aagatggcga tgatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtag 5641 atccaaaaaa gaagagaaag gtaataagaa gttcctattc tctagaaagt ataggaactt 5701 cagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 5761 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 5821 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 5881 acaaataaag catttttttc actgcgaatt ctcgtaaaac tcttcatggc agtagttatt 5941 attctctctc tctctctttt tcttttttct tgagacagga tatttctctg ttgcccaggc 6001 tggagtgcag tggcacagtc ttggctcact gcagcctgga cctcctgggc tcaagccatc 6061 ctcccacctc agcctcccaa gtagctgggg ctacaggcac atggccacca ggccagataa 6121 tttttcattt ttgtagagac tgagtctcac catgttaccc aggtttatta ttctcatttt 6181 ttagatgaag agactgaggt ccagagaagc tcaatgactt gcctagtttt acaaatctcc 6241 tgccatcaca tacccctcag cgtccttaat aagagggagg ccaccaacta tgtgctgggc 6301 actgtggtgg atgctggagc tatagggttg agtatataag aaatggtgtt gctggagcaa 6361 ctgttgcttg cttacctgac ctatctgaga attaattagc aggggaacat atttttgttt 6421 tcagattcaa tataagaact tgtgtgggca aaaataaaga tcagtagtaa taacagtagt 6481 tcccatttgc tgactgtact gtcctaagtg catatatata tacatacaca cacgcatacc 6541 tatactcctc taatactcaa aatgatcctg tttatgtatt gttaatatgc tcattttatt 6601 tttaaatttt tatttatttt tatttttatt tatttttgag acggagtctc attctgtcgc 6661 ggaggctgaa gtgcagtggt gcgatctcag ctcagtgcga cctccgcctc ccgggttcaa 6721 gtgattctcc tgcctcagct ccggactagc tgggattaca ggcgcccgcc tccacgccca 6781 gctaattttt gtatttttag tagagatggg gtttcgccat gttggccagg ctggtctcgt 6841 actcctgacc ttgagtgatc cacctgcctc ggcctcccaa agtgctggga ttacaggcat 6901 gagccaccgc gccgggctaa tatgctcatt ttagtgaggc aaaaatagag gctcagagtc 6961 tgatttgtac aaaactacag agcagttaag tgtcctctca gatgtgtacc ctgatctggg 7021 tgactctagg actctaggtc tcaactgtta caaccagtta agggtttggg gaagcactgg 7081 gccaagagtc aggaaaatgg aagccacagg tagtgcaagg tcttgggaat gggacgtctg 7141 gtccaaggat tcacgcgatg actggaaccc gaagagccgg ggcccggttt acggccgcca 7201 tgaagcaacg cgcgccggta ggtttgggaa tcagggagcc ctctgaatag gagactgagt 7261 tgggagggaa aggggcttcg ctgggggagc ctcggcttct tctgggagaa aattcccacg 7321 gctacctagt gagcctgcaa actggtaggc gccggcgtag gcgcgcgggc ggggccgggg 7381 gcggggcctg cggggcgtgg cggggcgggc agagggcggg gcctgcttct cctcagcttc 7441 aggcggctgc gacgagccct caggcgaacc tctcggcttt cccgcgcggc gccgcctctt 7501 gctgcgcctc cgcctcctcc tctgctccgc caccggcttc ctcctcctga gcagtcagcc 7561 cgcgcgccgg ccggctccgt tatggcgacc cgcagccctg gcgtcgtggt gagcagctcg 7621 gcctgccggc cctggccggt tcaggcccac gcggcaggtg gcggccgggc cctgaggcgc 7681 gggatcgatc catgacaccc ccccgccccc catatctcac tataattcac tgtcaaatgg 7741 cttttatgat ttggagtttg gggatacttt taaagacagg gtcttggtag ctcctagatg 7801 gcttgggatt cacttagtaa cccttgctgg cctaaaattc aggaacttct acctcatctt 7861 cccaagtact gggttagtag ggatgtccag catatttagc ttgctttaat gattttgctg 7921 tacttttttc tgtgtgtaca ggtgcatgta tatttataaa actaacataa tatattttac 7981 ccccaaagta ccataaccct aattaatgct attaaatgtt ttgtatgctg ggctctgggc 8041 atactaatat aaccaatatt atacaatgca aaacaatttt ggaatatctt ctgtaaaatt 8101 atcttattag taagttgtaa ccatctcaga cttctaagtt tgagtttgta aaggatcagt 8161 tttttttggt gacctacaca cacacacaca cacacacaca cacacacaca cacagggggg 8221 tggattctga gaatttagag aatttagatg gttaatacca gtttttattt ttattctgtg 8281 tgatatttat ttattaagat ttatttattt attacatgta ggtacactgt atctgtcttc 8341 agacacacca gaagagggca tcagatctcg ttacagatgg ttgtgagtca ccatgtggtt 8401 gctgggattt gaactcagga cctctggaag aacagtcagt gctcttaacc gttgagccat 8461 ctcaccagcc ccgtgatatt ttatttaact tactagaata tactgtgtta aaacatattt 8521 taggggattg gggagatggc ccattctgta aagtgtctgc catgtcatct tcaggacctg 8581 cgtttgtaac cccagcatgg agaagacaga gacagggaga cccctgggct tcctggcaag 8641 ctagactagc aaatttggtt aactaggttc aatgagagac tcagtctcaa aacataagaa 8701 gtgaattttt tagacccaat ttcaatctct gaactccata cgtatgcaca tgtacctgca 8761 tataaatata tatacacacc cataaacaca tgcacacaca aaaaatggtt caaaagttct 8821 taaaatgtat aagagtagaa agtgtagagc cgggagtggt ggcgcacgcc tttaatccca 8881 gcacttggga gcagaggcag gtggatttct gagttcgagg ccagcctggt ctacagagtg 8941 agttccagga cagccagagc tatacagaga aatcctgcct caaaaaacca aaaataaagt 9001 gtatatacta atctttcatg cctaaaatat ttatttttgc cacatttttc atacctcttt 9061 aatatttttc tttttctctt tgtgcttcaa aggaaatttt gtgtcatgtt ttacttttca 9121 ttcttgactc tattcagcag taagacgcag catggctttc ttagagaagt ataatctatt 9181 acaaaacggt aacatttaac tgctctacat accctcttgt agctaaattc ctctgttaaa 9241 ctaagactta ttatctaaac gtaattaaat gttgatatct tataatgctc cactttgaaa 9301 cagctgtctt tcttttgcag attagcgatg atgaaccagg ttatgaccta gatttgtttt 9361 gtatacctaa tcattatgcc gaggatttgg aaaaagtgtt tattcctcat ggactgatta 9421 tggacaggtt agtaagacct cgattgaagt tatttttaga gggtgtgtgt gtggtgtgtg 9481 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtaagagag agaggagagg catgtgtgat 9541 atatatgtga agtgtattgc atgtacatgg aatccagaga aggacattag gtgtcctact 9601 ctgttgctct cttattccct tgaatcagtg tctcttgctg aacctggggc atacatactg 9661 atttggctag gctgcctggc tagcaagacc cagggatctg cttgtctctg ccccccagtg 9721 ctgacgttac aagcaggtgc aaccacatct ggtttttata tgggtactgg gggatctgaa 9781 cttagggcct gatgcgtgcc caagtgctct cacccgccaa gccacctctc cagccaaagg 9841 gttttttttc ttgtattaat tctaacttag caactgttga gtagttgatg tttgaatata 9901 agctaagcca acttattaga ataaatgttt tctatttttt acttgtatgt gtgagtgcct 9961 gcatgtatgt atgtgaagca tatgtattcc aggatgtcag aattgtgatg gattccctgg 10021 aactggagtt acagaccatt gtgaaccact gtgtatgggt gctgggaact gaactttagg 10081 tcctctgcaa aaacagtaag gatcttaact gataaactat ctttctagcc ctaaggcttt 10141 ctacttttaa agttcgtttc tctttctctc tctctctctc tctctctctc tctctctccc 10201 tctccctccc tcctcctcct cctcctcttt cccccctctc gcctcctctt tctctctccc 10261 ctccctctct ctctctcaca cacacacaca gacagagaga ggagagagaa aggaagcctt 10321 actaattaat tacctagttt tagacataac tagtgggaat ccatagtaag taaatcaaca 10381 ttcttgaaga gaggttagaa gaattggctc tcgttgtcca tcgaggctgt ataagattat 10441 tcttctgttt tctcagctta ccatacatat tttctacgta gacaatacca attcaaatac 10501 actgtacgta aagtatatgc gcacacacac acacacacac acacacacac acacacaagt 10561 tacatattgt catatagtac taaaatttat ggactttttc atattaatac atctagagct 10621 atcatgtttt ttatctttta aaaattactt tacggggcta cagagatgtc tcagcagtta 10681 ggagcactga ctgctcttcc agaggtcctg agttcaattc ccagcaacca catggtggct 10741 cacaaccatc tgtaatggga tctgatccac tcttatggtg tgtctgaaga cagtgacagt 10801 gtactcatat acatgaaata aatctttaaa aaattacttt accatcaggg tatatatata 10861 tatatatatg tgtgtgtgtg tgtgtgtgtg tatgaagtat ttgctatttg agagggtctc 10921 actgtttagc tcagtctggc ttggaacgtt ctgtatagcc cagactggcc tcaactcagc 10981 agtccccctg aaaggctctc atatgtggag attacaggta tgtgctactg tgcccagccc 11041 tacatgttac tttgtttttg aaatagggta tcaaatagct tagactggct tctaagtcag 11101 tgtgtagcta aggatggtct gagcttctga acctgctact tcaactcctg gtgtgccatc 11161 atggccagtt tgcacagttg tgagagtaga actcaggacc tcctatgtgt tactagacat 11221 gttctctacc agctgagcta catcccaatt cgcctacatt tacttttatt ttttaaaaga 11281 tttatttatg tatacagcac tctgcctgca tgtacatctg catgccagaa gagggcacca 11341 gatctcatca tacatagttg tgagccacca tgtggttgct ggggattgaa ctcagggcct 11401 ctggaagagc agtcagtgct cttaacctct gagccatctc tgtagccccc tacatttatt 11461 ttaaagatta taggctattc tattcataga tgtgccataa ctgatttaac cagatcttca 11521 ttattttgta cttagtagag tatattagag ggaatgaatc ttaaatacac attttgacac 11581 ttgctcagct ttagacaagt aaaaatcact ggtcaaggaa atggtgctta gtggatgctt 11641 ctgaactgtc aaattgtcct ccaacaagtt acatcatttt atacttccaa cgttatgtga 11701 gagttcattt ttctttaggc tcttctagta ttttatattt gagaaataaa atttaaccaa 11761 aatttgccaa tctagtaagc ttgtttttat ttgtatcttt aaattttatg atagatgtta 11821 tagtgtactc tcctctcccc tcccctcccc tctcctcctc agcaggatct tgttatgtag 11881 ccctaaggga tctaatactt actctgtagt ccaggctgtc cttcagctca tgagacacac 11941 catcatgctt ggcatgtttc ttgttctgaa aggtatctgg ttttaatttt ggttgagctg 12001 gtgtgttggt gtatatctgt aatctcagca caggcaagta gagacaagga ttatgattac 12061 agtccagcct gggctattta gagggattct ctttaatctt tcctcatgcc ccaaaatctt 12121 acctttggta tatgaaaaat agtctccact tctgcaaaat attgctttat gaagtaagaa 12181 ttaattccct tcatagagac aaggaatgtg tcctgtaaaa gtttaatgtg taagaagtat 12241 ttgttataaa agataaatat tcagaatctt ctttttaatt cctgatttta tttctatagg 12301 actgaaagac ttgctcgaga tgtcatgaag gagatgggag gccatcacat tgtggccctc 12361 tgtgtgctca aggggggcta taagttcttt gctgacctgc tggattacat taaagcactg 12421 aatagaaata gtgatagatc cattcctatg actgtagatt ttatcagact gaagagctac 12481 tgtgtaagta taattaactt ataattaaaa aaatagggcc attctagttt tatctatatt 12541 ttttttaaac ttgtgcaaac tatgctacat aatcaactta cttgattatg taatatatat 12601 agtaatagtt ggaattacta ttattacata tttatttttc tgtgctgggg tagaacccag 12661 ggaactaagt actgtaataa ccgagcttca ttcatcctag tcatttttaa tttttttttt 12721 tttttttgtt ttttcgagac aaggtttctc tgtgtagccc tggctgtcct ggaactcact 12781 ctgtagacca ggctggcctc gaactcagaa atctgcctgc ctctgcctcc cagtgctgag 12841 attaatgacg tgcaccacca ctgcctggca tttttttaaa gttttaattt gagacccttt 12901 ctcactaact tgctcacact atcctcgaac tcattacgtg ggctggtcta gaaatttcag 12961 tcaacttgcc tcagcttctc aagtagctgg gattacaggc aggtgctacc acttctggcc 13021 atgatcattg ttgttgttgc tattattatt attattattt cactgatact tcatatcaca 13081 acacagaata gtaactgtag ttcatgacca acaaaaaagt tgtacagttt ctctgttgtc 13141 ttattttgat ctggattttg atttgtttca tggcactggg caggctaatc tcaaactgca 13201 tggctcaaat agtccttctg tcttaatctc ccatgttctg gaagtatagg tatcactaca 13261 cctagctgta ttttcatatt ttaaaaaaaa cgcctaagaa ttgctattga atgaggcgga 13321 gagatggctc agcagtcaca agtacttgta gaggactggg gtttggttac tagcacctca 13381 aagatggctt acaattatct gtagctccaa ttccagagga tctgtgtggt aaagctgcat 13441 gtatatgggc atgcaggtaa aactgctaaa catataaaaa tagctcagtc ttaagagaat 13501 tggtcgacgt aagctcattc gagtcgactt tttcgccctt tgacgttgga gtccacgttc 13561 tttaatagtg dgactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 13621 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 13681 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 13741 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 13801 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 13861 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 13921 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 13981 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 14041 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 14101 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 14161 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 14221 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 14281 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 14341 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 14401 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 14461 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 14521 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 14581 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 14641 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 14701 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 14761 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 14821 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 14881 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 14941 ccgctaccag gtcgagtagg gataacaggg taatggatcc tcgttgggca cctgcactca 15001 tgtacacagg cccacatata gatgtacaca cgcatatcat tcaaaataga aattatttat 15061 acaagtagtg cgttggagag ctggctcagg agttgttaat gcttgctacc cttgcaaagg 15121 acccaagttc aatttccagc acctgtgttg ggcatctcac aaccacatgt aaatctagtt 15181 tcaggggatt tcacccctct tctgaactct tcaggcactt atacccacaa atggacatct 15241 ccacatagag acacatactt ttaaaaaagt aatctagggt ctagagaaat ggttatagac 15301 cacggactgg gatcagttcc cagaagccac atcctgtggc tcacaattgc ctgtgaatca 15361 agttctagat ctggtggcct cgtctagcct gcagtcatca acaaacacat gcacacatcc 15421 atacacacat aatttaaaag aaagcgaaag taacctataa tgctaaacaa agcacattct 15481 cagtgtagac tgcagtggtg atgaaccttg aaaatatatt aatagaagcc agacacgtaa 15541 gtcacaaatg gtataactca atttatatga aattcccaga gagtccaaag ttaaaaaaaa 15601 caaaaaggca gattattggt tgtcagtagc tacagagatg ggacattgaa gatattaata 15661 ggtatagggt tgaggattca tggcctttcc ctcttccatg ttagcatgtc tattgttgtt 15721 aaccttattc aactcatgtt agggcaatca tattgatgag acttaatgag tatagcttga 15781 cattcctaga agacagagtc tcacatcaga ctctctgatc ctctgactct tagaatcttt 15841 caacaccctc ttctgaaatg atctctgagc ctttggttgt gactgaatta tgagaaagaa 15901 ttttatgttg aatgaatttt acttcagcat ttttacaaaa ctgaagctgg ggatggcgtt 15961 tcatgcgtgc agtccctacc atttgcctat aagcctggag gaggcagaag tagaattagg 16021 cttttcaggg acatcttcag ctgcatcatg agcttgagga caacttgagc tacataagat 16081 tcccatcccc acccccacac acacacacac atctagtatg tacctagtat gcagtctttt 16141 catatgttta aaatgtagaa aggtatatgt gaagtctccc ttttccccac aacttagtcc 16201 ctccaaagta ttgcatcagt ttccttgtac atgattttat gcatatataa gatacactat 16261 ttctctctct ctctctctcc ttccctcctt ccctccaacc tctgggtatg agttgagggt 16321 tatgtatgtt tcaagttgta ataagaattg ccactcaaac atttcatccc attttaagtt 16381 aagtttgtct gattttccac tttagaaggg cagccatgat ggctcatgcc tataatccca 16441 tcctagcact tgtaagccta atacataaga ctggaggatt gtcatcagct tgaggctagc 16501 ctggtctacc taatgagttc cagccagcca agagtagaat ataagaccct gtctccaaaa 16561 aatgtaccaa aggggctgga ggggtaactc agtggttaag aacagtagct gctcttccag 16621 aggacctggg ttcaattccc agcacccaca tggcagcttg caactatctg taactgtcca 16681 gctttgggat atccgacacc attataccaa ttacataaaa tgaaaaaatt gtaaaatgtg 16741 ctaaaatgag tggggagggg gagcatggtg tggttctgtg ggcttgggat tttgccatga 16801 atccatgact atatctcagc aatatgtgga ttatttctgg aaacctgtgg ggcatgggtg 16861 tatgggaaga ggggagagag agagagcccg catccagcca gagttcctgt gctctgggca 16921 ggcggacgtg ggaggactgc tggacgattt ccacttggcc ctgggtgggc atctggctgt 16981 gtgaagccac tgaccccact cagcgggggg tagataaggg gcatttccca taccaggttc 17041 tcagactcca gcattccacg ctggatacag cagaagagct gagaacagaa taggggcccc 17101 agggagacac tctcgtccca gagataaaga aggcagaggg agagagattc ctacgcaggc 17161 gagaggcctt agtctgggcc ttgatgaaca gagacggtct atggttttag agctttattg 17221 tagaaaggca gggggaaaga gagaaggtag aaagagagag accagccatg gccaagagga 17281 gagaaggggg gaaaggagag agagaagaaa ggctagagag taagaaaggt taaagagaca 17341 gaagtaaaag cttagagtaa gagagagaga gagagcaaga gggagagtaa gaaaagtaaa 17401 agcttaagag agcgaggagg ggccagcccc tctaatagtg ggcttgttat cttgttgttg 17461 ctaggtaact gtgaagcgtc tagcctgaag gccagaagct tgggacattg tctatgtgac 17521 tgaaagccat gagcctctac tgtgggggct gtggaggtgg taacttggac aggagccagg 17581 ggttccagga gacatgaggg aatgccttct gtcccatgta ggtaggaatt accaccaccg 17641 gctttcaccg tagttcactg gagactgttt gtacatagcc cattgcccca cagaaaccag 17701 gcactcactg tttcatttgc ataagaatta gaaggcttta ttttggctgg gattggtgac 17761 ttgccccaga aattccagct tttgggaagc cgaatcctgg agaaccagga gttcaaggtc 17821 atcctcaggt acctcatttg ttcaagacca gcctgtgcta ccatgagacc ttgtccctac 17881 tcccctcaac cccaaaacac gctgttttca ttttataggt tgtactaacc atggaggatg 17941 gtcatttgcc tttctcttga tttcagaagc gtagcgcggg ctttgcacca tgatctagtg 18001 tttccagata ggcttgcctc tccacagcca ggcttatcct gcttcagtcc catgtttggc 18061 aagggcaaca tatgactgaa gaacttgcat agccttatgg tgtatcatga ctcttagccc 18121 cagtttataa ctgtctaaca gctggtggct tttgtgaaaa aggaaagcat catttgaact 18181 tttaactttt cagctgtttg ttttgacttt gcataaatac tgagactagc taggaaatga 18241 gcttttgttt catttgctaa aaatgatttt tttttcatga ggcaatttca agcaatttga 18301 atttagcaat ctctacttgt ctgaaaagaa gttattaatt aaaaaaaaac aaacaaacag 18361 gaacctatta tgctggctag tcacatgaaa gaatccaaat gaacacagtc aaggctgaat 18421 caacacatga aattattttc taattcccca aattacattt atgctaatta gtatgtagta 18481 aaacatttta attgtttcca atgttaaaca ttacagaaat aattttgatg ttttcctaat 18541 aacccagcct ttgatcatgt ttgcatttat ttgattgttg tcagtttatt aaataattaa 18601 gtaagagcaa caagataaaa tgacttcatg agagtccttt tctgtaactt agaacgtcag 18661 tagtcatagg aactgcggtc gttcccttac ctttcagaag taggctaaag agttgaacgc 18721 aaaggtggtt tttcgaatct tcctgatcct taagccccca ctaggtttat attctatatt 18781 tgatatcgga ttcgaacccc tcgagcttag gatccggaac ccttaatata actaagttat 18841 taggtccctc gaagaggttc actagtactg gccattgcgg ccgcttcgag gctgcctcgc 18901 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 18961 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 19021 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 19081 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 19141 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 19201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 19261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 19321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 19381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 19441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 19501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 19561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 19621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 19681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 19741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 19801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 19861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 19921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 19981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 20041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 20101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 20161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 20221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 20281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 20341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 20401 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg 20461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 20521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 20581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 20641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 20701 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 20761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 20821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 20881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 20941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 21001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 21061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtg //