LOCUS Ple80\(pEMS1060) 19763 bp DNA circular 17-NOV-2008 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2319 5'- TGGTCCAGAGAGTTTAAGAGCCCT -3' Tm= 56.5 Deg C Antisense primer: oEMS2308 5'- TGTTCCAATAGGCTCAGCGTTTAT -3' Tm=56.1 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 435 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|499180842| COMMENT VNTDBDATE|499180988| COMMENT LSOWNER| COMMENT VNTNAME|Ple80 (pEMS1060)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#205#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #207=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -953 100) COMMENT (CObjectList) (CObjectList)) COMMENT #208=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -186 100) COMMENT (CObjectList) (CObjectList)) COMMENT #209=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -395 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 COMMENT (CObList #210=(AnlzFontBackColorItem 206 1098 45977377) COMMENT #211=(AnlzFontBackColorItem 1099 1506 50305535) COMMENT #212=(AnlzFontBackColorItem 1722 1917 41976063) COMMENT #213=(AnlzFontBackColorItem 1531 1721 50305535) COMMENT #214=(AnlzFontBackColorItem 1507 1530 43633766) COMMENT #215=(AnlzFontBackColorItem 1942 2389 41976063) COMMENT #216=(AnlzFontBackColorItem 1918 1941 43633766))) (CObList) 1699618931 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers misc_recomb 13605..17416 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker 18757..19757 /vntifkey="22" /label=AmpR misc_marker 12154..13579 /vntifkey="22" /label=AmpR misc_recomb 6337..12158 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 4559..6336 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 116..149 /vntifkey="21" /label=LoxP misc_feature 17457..17490 /vntifkey="21" /label=LoxP misc_feature 4521..4553 /vntifkey="21" /label=FRT:\with\mutation\-\A\deletion misc_signal 4245..4317 /vntifkey="87" /label=NLS misc_signal 4470..4520 /vntifkey="87" /label=SV40\early\mRNA\polyA\signal misc_signal 3516..4235 /vntifkey="87" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 2423..2456 /vntifkey="21" /label=FRT CDS 2457..3485 /vntifkey="4" /label=Cre misc_feature 13586..13603 /vntifkey="21" /label=I-Sce\I misc_feature 207..1099 /vntifkey="21" /label=region\1 misc_feature 1100..1722 /vntifkey="21" /label=region\2 misc_feature 1723..2390 /vntifkey="21" /label=Prom\region misc_feature 207..2390 /vntifkey="21" /label=Ple80\(GABRA6-D) primer 1508..1531 /vntifkey="27" /label=oEMS2319 primer 1919..1942 /vntifkey="27" /label=oEMS2308 BASE COUNT 5348 a 4388 c 4421 g 5606 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatataac 121 ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa 181 gctcgaaggg gttcgaattc gagctctcac ataataacat gaagaacctg tattcccatg 241 aaatcctctg acctttccaa ggcacttcaa cattttattt gtactcttgc tctgagaatg 301 tccctgtgca tgacagtagc tttggagttt acatcttatc caagtccaac agaatccatg 361 tttaattgac ttccattctg cttgtgcttc tgtaacattt ccccagagca catcactggg 421 cttgaaccca aaattctgtc ctctcttttc tatactccaa gcatttgata aataggcaga 481 ttccagggca tgagtagcac tgccagaaga caatgctcag tagtccccta gtggcctgaa 541 agagttcaca cagtttacta acgtgcttat tctgttccat ggaggtgtga gagactcatt 601 aagcagcagt tcattaagta actgccaatt agcttgatcc tttctggaat ggccaggaag 661 caagtccagg attccacctt ttctaggttt tctctcttgt gggtcacacg acctatctga 721 gtgtcctact gtattgtcac aatctgaaat tctgctttca tatttggaat acagccttct 781 gagttaagct cctttgagaa cccagtgata gaaaacattt aagttgcctt gaagaaaaaa 841 gaggaattta taataaagac acaacccttt cttgggggaa accaaaggca gaaatgacac 901 ctagattatg aaggaataaa atcagggagg ctgtcagaga tccagacagc tgctctctca 961 gattttgggg ttgcagtgac tcttgcctat gcctccttcc atggggctgc tgcagttcgg 1021 tttctttctg tctctgttgt tgcattaact cagccaaaca tgcctgcccc caaaaccgac 1081 agccttagga aatgacactt tctgaaggtg atctttctca aaatataaaa aaccacttga 1141 taaataaaag ttaaattgaa tgaataccag ttgagcaaat ataaatcata cattcatata 1201 catgtgctct ttaagaagtg tctttttggt tctttggtat ttgtgttttg aagattgaaa 1261 cagctattcc ctgcctatat tttttggggg tcccagaatg ccctctcatg tactcaactt 1321 tctactccta accaagccaa aacaatttaa agctgcagtc cagagaagtg tcgctttatt 1381 gtcctttgct tcccagagca attaggtcct tattgcaccg gatcctccaa agggatcaca 1441 tggggattta gtcacagtgt ccactgagat ggtgggtatt atttaacaac aatataaaaa 1501 ctattattgg tccagagagt ttaagagccc taactttaga gaaaggatca gatctgatat 1561 aatgcacaaa gcacaatgta taaaagagtg tattttggca tcagaggtaa taatagctct 1621 gagtcctcag gtaataacat atttttgtga gtctcattct cctcttttgt aaaatgaaca 1681 taaaaaggct attttgtaaa gtagttgcac agattagaga caatggccgt gagttgttct 1741 gttgtgttac aagtgctggt aaataaatca tgagcaataa tctttaaata cataagggtg 1801 tggctgtagc tagtaatgct gatgaaaatc agaaagggaa tccaagaggg tcacaatagt 1861 gttagacttt ttaaacagta agagctgtgt aggaatcctt ctgtgaacca taaaaataat 1921 aaacgctgag cctattggaa cataatctca atcagcaaga tgccaatgct ttccagattt 1981 cctcacaggg ccctgctaat tccatgccaa aactggcagt ggatttcttc cttctaatct 2041 cccttaatgc cttattcgat tattgtcact gctctccgac catgcactgt cccttcagac 2101 tctcctttcc caactgagtc agtcagagga gcctgggtgt ctgcaggaca taatctaaga 2161 ccacaaacca ccttgttcca cgtgagaagg aaacaagaaa gaagggagcc aggggaatcc 2221 tgcaaatttt taggcaacct ctttatctat tggattactg acttgaggca aacaaggaac 2281 agagatatga agggtttgaa agatttctcc agttgattgg cagagaagag ctggctagca 2341 gggaggacga ccctaggagg gtgaattctg catttcagtg cactgcagga acgcgtcacg 2401 tggcgctacc ggactcagat ctgaagttcc tattctctag aaagtatagg aacttcatgt 2461 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg 2521 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct 2581 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga 2641 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg 2701 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt 2761 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa 2821 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt 2881 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg 2941 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg 3001 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa 3061 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg 3121 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg 3181 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg 3241 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga 3301 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg 3361 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca 3421 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg 3481 gcgatggtac cgcgggcccg ggatccatcg ccaccatggt gagcaagggc gaggagctgt 3541 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 3601 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 3661 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 3721 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 3781 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 3841 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 3901 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 3961 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 4021 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 4081 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 4141 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 4201 ggatcactct cggcatggac gagctgtaca agttaagcgg ccgcgatcca aaaaagaaga 4261 gaaaggtaga tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtaagcg 4321 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 4381 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 4441 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 4501 taaagcattt ttttcactgc gaagttccta ttctctagaa agtataggac ttcgaattct 4561 cgtaaaactc ttcatggcag tagttattat tctctctctc tctctttttc ttttttcttg 4621 agacaggata tttctctgtt gcccaggctg gagtgcagtg gcacagtctt ggctcactgc 4681 agcctggacc tcctgggctc aagccatcct cccacctcag cctcccaagt agctggggct 4741 acaggcacat ggccaccagg cccagataat ttttcatttt tgtagagact gagtctcacc 4801 atgttaccca ggtttattat tctcattttt tagatgaaga gactgaggtc cagagaagct 4861 caatgacttg cctagtttta caaatctcct gccatcacat acccctcagc gtccttaata 4921 agagggaggc caccaactat gtgctgggca ctgtggtgga tgctggagct atagggttga 4981 gtatataaga aatggtgttg ctggagcaac tgttgcttgc ttacctgacc tatctgagaa 5041 ttaattagca ggggaacata tttttgtttt cagattcaat ataagaactt gtgtgggcaa 5101 aaataaagat cagtagtaat aacagtagtt cccatttgct gactgtactg tcctaagtgc 5161 atatatatat acatacacac acgcatacct atactcctct aatactcaaa atgatcctgt 5221 ttatgtattg ttaatatgct cattttattt ttaaattttt atttattttt atttttattt 5281 atttttgaga cggagtctca ttctgtcgcg gaggctgaag tgcagtggtg cgatctcagc 5341 tcagtgcgac ctccgcctcc cgggttcaag tgattctcct gcctcagcct ccggactagc 5401 tgggattaca ggcgcccgcc tccacgccca gctaattttt gtatttttag tagagatggg 5461 gtttcgccat gttggccagg ctggtctcgt actcctgacc ttgagtgatc cacctgcctc 5521 ggcctcccaa agtgctggga ttacaggcat gagccaccgc gccgggctaa tatgctcatt 5581 ttagtgaggc aaaaatagag gctcagagtc tgatttgtac aaaactacag agcagttaag 5641 tgtcctctca gatgtgtacc ctgatctggg tgactctagg actctaggtc tcaactgtta 5701 caaccagtta agggtttggg gaagcactgg gccaagagtc aggaaaatgg aagccacagg 5761 tagtgcaagg tcttgggaat gggacgtctg gtccaaggat tcacgcgatg actggaaccc 5821 gaagagccgg ggcccggttt acggccgcca tgaagcaacg cgcgccggta ggtttgggaa 5881 tcagggagcc ctctgaatag gagactgagt tgggagggaa aggggcttcg ctgggggagc 5941 ctcggcttct tctgggagaa aattcccacg gctacctagt gagcctgcaa actggtaggc 6001 gccggcgtag gcgcgcgggc ggggccgggg gcggggcctg cggggcgtgg cggggcgggc 6061 agagggcggg gcctgcttct cctcagcttc aggcggctgc gacgagccct caggcgaacc 6121 tctcggcttt cccgcgcggc gccgcctctt gctgcgcctc cgcctcctcc tctgctccgc 6181 caccggcttc ctcctcctga gcagtcagcc cgcgcgccgg ccggctccgt tatggcgacc 6241 cgcagccctg gcgtcgtggt gagcagctcg gcctgccggc cctggccggt tcaggcccac 6301 gcggcaggtg gcggccgggc cctgaggcgc gggatcgatc catgacaccc ccccgccccc 6361 catatctcac tataattcac tgtcaaatgg cttttatgat ttggagtttg gggatacttt 6421 taaagacagg gtcttggtag ctcctagatg gcttgggatt cacttagtaa cccttgctgg 6481 cctaaaattc aggaacttct acctcatctt cccaagtact gggttagtag ggatgtccag 6541 catatttagc ttgctttaat gattttgctg tacttttttc tgtgtgtaca ggtgcatgta 6601 tatttataaa actaacataa tatattttac ccccaaagta ccataaccct aattaatgct 6661 attaaatgtt ttgtatgctg ggctctgggc atactaatat aaccaatatt atacaatgca 6721 aaacaatttt ggaatatctt ctgtaaaatt atcttattag taagttgtaa ccatctcaga 6781 cttctaagtt tgagtttgta aaggatcagt tttttttggt gacctacaca cacacacaca 6841 cacacacaca cacacacaca cacagggggg tggattctga gaatttagag aatttagatg 6901 gttaatacca gtttttattt ttattctgtg tgatatttat ttattaagat ttatttattt 6961 attacatgta ggtacactgt atctgtcttc agacacacca gaagagggca tcagatctcg 7021 ttacagatgg ttgtgagtca ccatgtggtt gctgggattt gaactcagga cctctggaag 7081 aacagtcagt gctcttaacc gttgagccat ctcaccagcc ccgtgatatt ttatttaact 7141 tactagaata tactgtgtta aaacatattt taggggattg gggagatggc ccattctgta 7201 aagtgtctgc catgtcatct tcaggacctg cgtttgtaac cccagcatgg agaagacaga 7261 gacagggaga cccctgggct tcctggcaag ctagactagc aaatttggtt aactaggttc 7321 aatgagagac tcagtctcaa aacataagaa gtgaattttt tagacccaat ttcaatctct 7381 gaactccata cgtatgcaca tgtacctgca tataaatata tatacacacc cataaacaca 7441 tgcacacaca aaaaatggtt caaaagttct taaaatgtat aagagtagaa agtgtagagc 7501 cgggagtggt ggcgcacgcc tttaatccca gcacttggga gcagaggcag gtggatttct 7561 gagttcgagg ccagcctggt ctacagagtg agttccagga cagccagagc tatacagaga 7621 aatcctgcct caaaaaacca aaaataaagt gtatatacta atctttcatg cctaaaatat 7681 ttatttttgc cacatttttc atacctcttt aatatttttc tttttctctt tgtgcttcaa 7741 aggaaatttt gtgtcatgtt ttacttttca ttcttgactc tattcagcag taagacgcag 7801 catggctttc ttagagaagt ataatctatt acaaaacggt aacatttaac tgctctacat 7861 accctcttgt agctaaattc ctctgttaaa ctaagactta ttatctaaac gtaattaaat 7921 gttgatatct tataatgctc cactttgaaa cagctgtctt tcttttgcag attagcgatg 7981 atgaaccagg ttatgaccta gatttgtttt gtatacctaa tcattatgcc gaggatttgg 8041 aaaaagtgtt tattcctcat ggactgatta tggacaggtt agtaagacct cgattgaagt 8101 tatttttaga gggtgtgtgt gtggtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 8161 tgtaagagag agaggagagg catgtgtgat atatatgtga agtgtattgc atgtacatgg 8221 aatccagaga aggacattag gtgtcctact ctgttgctct cttattccct tgaatcagtg 8281 tctcttgctg aacctggggc atacatactg atttggctag gctgcctggc tagcaagacc 8341 cagggatctg cttgtctctg ccccccagtg ctgacgttac aagcaggtgc aaccacatct 8401 ggtttttata tgggtactgg gggatctgaa cttagggcct gatgcgtgcc caagtgctct 8461 cacccgccaa gccacctctc cagccaaagg gttttttttc ttgtattaat tctaacttag 8521 caactgttga gtagttgatg tttgaatata agctaagcca acttattaga ataaatgttt 8581 tctatttttt acttgtatgt gtgagtgcct gcatgtatgt atgtgaagca tatgtattcc 8641 aggatgtcag aattgtgatg gattccctgg aactggagtt acagaccatt gtgaaccact 8701 gtgtatgggt gctgggaact gaactttagg tcctctgcaa aaacagtaag gatcttaact 8761 gataaactat ctttctagcc ctaaggcttt ctacttttaa agttcgtttc tctttctctc 8821 tctctctctc tctctctctc tctctctccc tctccctccc tcctcctcct cctcctcttt 8881 cccccctctc gcctcctctt tctctctccc ctccctctct ctctctcaca cacacacaca 8941 gacagagaga ggagagagaa aggaagcctt actaattaat tacctagttt tagacataac 9001 tagtgggaat ccatagtaag taaatcaaca ttcttgaaga gaggttagaa gaattggctc 9061 tcgttgtcca tcgaggctgt ataagattat tcttctgttt tctcagctta ccatacatat 9121 tttctacgta gacaatacca attcaaatac actgtacgta aagtatatgc gcacacacac 9181 acacacacac acacacacac acacacaagt tacatattgt catatagtac taaaatttat 9241 ggactttttc atattaatac atctagagct atcatgtttt ttatctttta aaaattactt 9301 tacggggcta cagagatgtc tcagcagtta ggagcactga ctgctcttcc agaggtcctg 9361 agttcaattc ccagcaacca catggtggct cacaaccatc tgtaatggga tctgatccac 9421 tcttatggtg tgtctgaaga cagtgacagt gtactcatat acatgaaata aatctttaaa 9481 aaattacttt accatcaggg tatatatata tatatatatg tgtgtgtgtg tgtgtgtgtg 9541 tatgaagtat ttgctatttg agagggtctc actgtttagc tcagtctggc ttggaacgtt 9601 ctgtatagcc cagactggcc tcaactcagc agtccccctg aaaggctctc atatgtggag 9661 attacaggta tgtgctactg tgcccagccc tacatgttac tttgtttttg aaatagggta 9721 tcaaatagct tagactggct tctaagtcag tgtgtagcta aggatggtct gagcttctga 9781 acctgctact tcaactcctg gtgtgccatc atggccagtt tgcacagttg tgagagtaga 9841 actcaggacc tcctatgtgt tactagacat gttctctacc agctgagcta catcccaatt 9901 cgcctacatt tacttttatt ttttaaaaga tttatttatg tatacagcac tctgcctgca 9961 tgtacatctg catgccagaa gagggcacca gatctcatca tacatagttg tgagccacca 10021 tgtggttgct ggggattgaa ctcagggcct ctggaagagc agtcagtgct cttaacctct 10081 gagccatctc tgtagccccc tacatttatt ttaaagatta taggctattc tattcataga 10141 tgtgccataa ctgatttaac cagatcttca ttattttgta cttagtagag tatattagag 10201 ggaatgaatc ttaaatacac attttgacac ttgctcagct ttagacaagt aaaaatcact 10261 ggtcaaggaa atggtgctta gtggatgctt ctgaactgtc aaattgtcct ccaacaagtt 10321 acatcatttt atacttccaa cgttatgtga gagttcattt ttctttaggc tcttctagta 10381 ttttatattt gagaaataaa atttaaccaa aatttgccaa tctagtaagc ttgtttttat 10441 ttgtatcttt aaattttatg atagatgtta tagtgtactc tcctctcccc tcccctcccc 10501 tctcctcctc agcaggatct tgttatgtag ccctaaggga tctaatactt actctgtagt 10561 ccaggctgtc cttcagctca tgagacacac catcatgctt ggcatgtttc ttgttctgaa 10621 aggtatctgg ttttaatttt ggttgagctg gtgtgttggt gtatatctgt aatctcagca 10681 caggcaagta gagacaagga ttatgattac agtccagcct gggctattta gagggattct 10741 ctttaatctt tcctcatgcc ccaaaatctt acctttggta tatgaaaaat agtctccact 10801 tctgcaaaat attgctttat gaagtaagaa ttaattccct tcatagagac aaggaatgtg 10861 tcctgtaaaa gtttaatgtg taagaagtat ttgttataaa agataaatat tcagaatctt 10921 ctttttaatt cctgatttta tttctatagg actgaaagac ttgctcgaga tgtcatgaag 10981 gagatgggag gccatcacat tgtggccctc tgtgtgctca aggggggcta taagttcttt 11041 gctgacctgc tggattacat taaagcactg aatagaaata gtgatagatc cattcctatg 11101 actgtagatt ttatcagact gaagagctac tgtgtaagta taattaactt ataattaaaa 11161 aaatagggcc attctagttt tatctatatt ttttttaaac ttgtgcaaac tatgctacat 11221 aatcaactta cttgattatg taatatatat agtaatagtt ggaattacta ttattacata 11281 tttatttttc tgtgctgggg tagaacccag ggaactaagt actgtaataa ccgagcttca 11341 ttcatcctag tcatttttaa tttttttttt tttttttgtt ttttcgagac aaggtttctc 11401 tgtgtagccc tggctgtcct ggaactcact ctgtagacca ggctggcctc gaactcagaa 11461 atctgcctgc ctctgcctcc cagtgctgag attaatgacg tgcaccacca ctgcctggca 11521 tttttttaaa gttttaattt gagacccttt ctcactaact tgctcacact atcctcgaac 11581 tcattacgtg ggctggtcta gaaatttcag tcaacttgcc tcagcttctc aagtagctgg 11641 gattacaggc aggtgctacc acttctggcc atgatcattg ttgttgttgc tattattatt 11701 attattattt cactgatact tcatatcaca acacagaata gtaactgtag ttcatgacca 11761 acaaaaaagt tgtacagttt ctctgttgtc ttattttgat ctggattttg atttgtttca 11821 tggcactggg caggctaatc tcaaactgca tggctcaaat agtccttctg tcttaatctc 11881 ccatgttctg gaagtatagg tatcactaca cctagctgta ttttcatatt ttaaaaaaaa 11941 cgcctaagaa ttgctattga atgaggcgga gagatggctc agcagtcaca agtacttgta 12001 gaggactggg gtttggttac tagcacctca aagatggctt acaattatct gtagctccaa 12061 ttccagagga tctgtgtggt aaagctgcat gtatatgggc atgcaggtaa aactgctaaa 12121 catataaaaa tagctcagtc ttaagagaat tggtcgactt tttcgccctt tgacgttgga 12181 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 12241 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 12301 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt 12361 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 12421 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 12481 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 12541 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 12601 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 12661 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 12721 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 12781 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 12841 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 12901 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 12961 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 13021 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 13081 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 13141 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 13201 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 13261 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 13321 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 13381 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 13441 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 13501 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 13561 aaaaaaccac cgctaccagg tcgagtaggg ataacagggt aatggatcct cgttgggcac 13621 ctgcactcat gtacgcaggc ccacatatag atgtacacac gcatatcatt caaaatagaa 13681 attatttata caagtagtgc gttggagagc tggctcagga gttgttaatg cttgctaccc 13741 ttgcaaagga cccaagttca atttccagca cctgtgttgg gcatctcaca accacatgta 13801 aatctagttt caggggattt cacccctctt ctgaactctt caggcactta tacccacaaa 13861 tggacatctc cacatagaga cacatacttt taaaaaagta atctagggtc tagagaaatg 13921 gttatagacc acggactggg atcagttccc agaagccaca tcctgtggct cacaattgcc 13981 tgtgaatcaa gttctagatc tggtggcctc gtctagcctg cagtcatcaa caaacacatg 14041 cacacatcca tacacacata atttaaaaga aagcgaaagt aacctataat gctaaacaaa 14101 gcacattctc agtgtagact gcagtggtga tgaaccttga aaatatatta atagaagcca 14161 gacacgtaag tcacaaatgg tataactcaa tttatatgaa attcccagag agtccaaagt 14221 taaaaaaaac aaaaaggcag attattggtt gtcagtagct acagagatgg gacattgaag 14281 atattaatag gtatagggtt gaggattcat ggcctttccc tcttccatgt tagcatgtct 14341 attgttgtta accttattca actcatgtta gggcaatcat attgatgaga cttaatgagt 14401 atagcttgac attcctagaa gacagagtct cacatcagac tctctgatcc tctgactctt 14461 agaatctttc aacaccctct tctgaaatga tctctgagcc tttggttgtg actgaattat 14521 gagaaagaat tttatgttga atgaatttta cttcagcatt tttacaaaac tgaagctggg 14581 gatggcgttt catgcgtgca gtccctacca tttgcctata agcctggagg aggcagaagt 14641 agaattaggc ttttcaggga catcttcagc tgcatcatga gcttgaggac aacttgagct 14701 acataagatt cccatcccca cccccacaca cacacacaca tctagtatgt acctagtatg 14761 cagtcttttc atatgtttaa aatgtagaaa ggtatatgtg aagtctccct tttccccaca 14821 acttagtccc tccaaagtat tgcatcagtt tccttgtaca tgattttatg catatataag 14881 atacactatt tctctctctc tctctctcct tccctccttc cctccaacct ctgggtatga 14941 gttgagggtt atgtatgttt caagttgtaa taagaattgc cactcaaaca tttcatccca 15001 ttttaagtta agtttgtctg attttccact ttagaagggc agccatgatg gctcatgcct 15061 ataatcccat cctagcactt gtaagcctaa tacataagac tggaggattg tcatcagctt 15121 gaggctagcc tggtctacct aatgagttcc agccagccaa gagtagaata taagaccctg 15181 tctccaaaaa atgtaccaaa ggggctggag gggtaactca gtggttaaga acagtagctg 15241 ctcttccaga ggacctgggt tcaattccca gcacccacat ggcagcttgc aactatctgt 15301 aactgtccag ctttgggata tccgacacca ttataccaat tacataaaat gaaaaaattg 15361 taaaatgtgc taaaatgagt ggggaggggg agcatggtgt ggttctgtgg gcttgggatt 15421 ttgccatgaa tccatgacta tatctcagca atatgtggat tatttctgga aacctgtggg 15481 gcatgggtgt atgggaagag gggagagaga gagagcccgc atccagccag agttcctgtg 15541 ctctgggcag gcggacgtgg gaggactgct ggacgatttc cacttggccc tgggtgggca 15601 tctggctgtg tgaagccact gaccccactc agcggggggt agataagggg catttcccat 15661 accaggttct cagactccag cattccacgc tggatacagc agaagagctg agaacagaat 15721 aggggcccca gggagacact ctcgtcccag agataaagaa ggcagaggga gagagattcc 15781 tacgcaggcg agaggcctta gtctgggcct tgatgaacag agacggtcta tggttttaga 15841 gctttattgt agaaaggcag ggggaaagag agaaggtaga aagagagaga ccagccatgg 15901 ccaagaggag agaagggggg aaaggagaga gagaagaaag gctagagagt aagaaaggtt 15961 aaagagacag aagtaaaagc ttagagtaag agagagagag agagcaagag ggagagtaag 16021 aaaagtaaaa gcttaagaga gcgaggaggg gccagcccct ctaatagtgg gcttgttatc 16081 ttgttgttgc taggtaactg tgaagcgtct agcctgaagg ccagaagctt gggacattgt 16141 ctatgtgact gaaagccatg agcctctact gtgggggctg tggaggtggt aacttggaca 16201 ggagccaggg gttccaggag acatgaggga atgccttctg tcccatgtag gtaggaatta 16261 ccaccaccgg ctttcaccgt agttcactgg agactgtttg tacatagccc attgccccac 16321 agaaaccagg cactcactgt ttcatttgca taagaattag aaggctttat tttggctggg 16381 attggtgact tgccccagaa attccagctt ttgggaagcc gaatcctgga gaaccaggag 16441 ttcaaggtca tcctcaggta cctcatttgt tcaagaccag cctgtgctac catgagacct 16501 tgtccctact cccctcaacc ccaaaacacg ctgttttcat tttataggtt gtactaacca 16561 tggaggatgg tcatttgcct ttctcttgat ttcagaagcg tagcgcgggc tttgcaccat 16621 gatctagtgt ttccagatag gcttgcctct ccacagccag gcttatcctg cttcagtccc 16681 atgtttggca agggcaacat atgactgaag aacttgcata gccttatggt gtatcatgac 16741 tcttagcccc agtttataac tgtctaacag ctggtggctt ttgtgaaaaa ggaaagcatc 16801 atttgaactt ttaacttttc agctgtttgt tttgactttg cataaatact gagactagct 16861 aggaaatgag cttttgtttc atttgctaaa aatgattttt ttttcatgag gcaatttcaa 16921 gcaatttgaa tttagcaatc tctacttgtc tgaaaagaag ttattaatta aaaaaaaaca 16981 aacaaacagg aacctattat gctggctagt cacatgaaag aatccaaatg aacacagtca 17041 aggctgaatc aacacatgaa attattttct aattccccaa attacattta tgctaattag 17101 tatgtagtaa aacattttaa ttgtttccaa tgttaaacat tacagaaata attttgatgt 17161 tttcctaata acccagcctt tgatcatgtt tgcatttatt tgattgttgt cagtttatta 17221 aataattaag taagagcaac aagataaaat gacttcatga gagtcctttt ctgtaactta 17281 gaacgtcagt agtcatagga actgcggtcg ttcccttacc tttcagaagt aggctaaaga 17341 gttgaacgca aaggtggttt ttcgaatctt cctgatcctt aagcccccac taggtttata 17401 ttctatattt gatatcggat tcgaacccct cgagcttagg atccggaacc cttaatataa 17461 cttcgtataa tgtatgctat acgaagttat taggtccctc gaagaggttc actagtactg 17521 gccattgcgg ccgcttcgag gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg 17581 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 17641 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc 17701 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg 17761 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 17821 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 17881 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 17941 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 18001 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 18061 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 18121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 18181 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 18241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 18301 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 18361 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 18421 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 18481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 18541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 18601 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 18661 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 18721 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 18781 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 18841 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 18901 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 18961 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 19021 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 19081 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 19141 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 19201 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 19261 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 19321 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 19381 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 19441 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 19501 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 19561 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 19621 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 19681 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 19741 ccccgaaaag tgccacctga cgg //