LOCUS Ple79\(pEMS1059) 18829 bp DNA circular 17-NOV-2008 SOURCE ORGANISM COMMENT PCR genotyping: Sense primer: oEMS2316 5'- ATGTGTCTTCCAACAGGTGAATACG -3' Tm= 56.0 Deg C Antisense primer: oEMS2308 5'- TGTTCCAATAGGCTCAGCGTTTAT -3' Tm=56.1 Deg C Cycling conditions: Step 1: 94 Deg C for 3 min Step 2: 94 Deg C for 1 min Step 3: 61 Deg C for 1 min Step 4: 72 Deg C for 45 sec Step 5: repeat Step 2-4 34 times Step 6: 72 Deg C for 5 min 327 bp product COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|499180241| COMMENT VNTDBDATE|499180241| COMMENT LSOWNER| COMMENT VNTNAME|Ple79 (pEMS1059)| COMMENT VNTAUTHORNAME|Russell Bonaguro| COMMENT VNTOAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#204=(AnlzFontBackColorItem 206 787 43633766) COMMENT #205=(AnlzFontBackColorItem 788 1455 41976063))) (CObList) 1699618931 COMMENT (CStringList) 0 0 (CObList))) FEATURES Location/Qualifiers misc_recomb 12671..16482 /vntifkey="86" /label=5'\arm\of\Hprt\homology /note="The 5 HPRT probe anneals to genomic sequence ~560bp upstream of the BamHI site at 9263" misc_marker 17823..18823 /vntifkey="22" /label=AmpR misc_marker 11220..12645 /vntifkey="22" /label=AmpR misc_recomb 5403..11224 /vntifkey="86" /label=3'\arm\of\Hprt\homology /note="Consists of part of Intron 2, all of Exon 3, and part of Intron 3" misc_recomb 3625..5402 /vntifkey="86" /label=HPRT\complementary\sequence /note="Consists of human HPRT promoter, human Exon1, Intron1, Exon2, and part of Intron2" misc_feature 116..149 /vntifkey="21" /label=LoxP misc_feature 16523..16556 /vntifkey="21" /label=LoxP misc_feature 3587..3619 /vntifkey="21" /label=FRT:\with\mutation\-\A\deletion misc_signal 3311..3383 /vntifkey="87" /label=NLS misc_signal 3536..3586 /vntifkey="87" /label=SV40\early\mRNA\polyA\signal misc_signal 2582..3301 /vntifkey="87" /label=EGFP\(mutated\stop\TAA\->\TTA) misc_feature 1489..1522 /vntifkey="21" /label=FRT CDS 1523..2551 /vntifkey="4" /label=Cre misc_feature 12652..12669 /vntifkey="21" /label=I-Sce\I misc_feature 207..1456 /vntifkey="21" /label=GABRA6-C /note="1040" primer 682..706 /vntifkey="27" /label=oEMS2316 primer 985..1008 /vntifkey="27" /label=oEMS2308 misc_feature 207..788 /vntifkey="21" /label=region\4 misc_feature 789..1456 /vntifkey="21" /label=Prom\region BASE COUNT 5077 a 4178 c 4230 g 5344 t ORIGIN 1 cggccgcacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 61 acgaggccct ttcgtcttca agaattaatt ccgatcatat tcaataaccc ttaatataac 121 ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa 181 gctcgaaggg gttcgaattc gagctctctt caagccttct ccagtatgat ctgattggac 241 aaacagtatc tagtgagaca attaaatcta acacaggtaa gaatttgacc aaaggatgga 301 aataatccct caggatgact ccagtgcaca ttttcaaaat atctatttct ttttccttcg 361 ccttagccat tctcagctaa gcatgctgtt ttgcagtgat gagtagcttt ccttaaaact 421 gattttgcaa ccaggtgcat ataaagattt gtgtaatctt ggaataagta gtttttttta 481 tgtatatgat ataaagaacc ctactgaaaa caataagttg atgtgtctct tcgctctatc 541 atctgctgat caagtagctt tctcatatac tgaaatgtac ctttgcttgt tttcttccag 601 tcaatgttaa tttaaaagtt gtcaagattt atattcaact gcattctttt tgcagactgt 661 cagcagtaat aatactgatg tatgtgtctt ccaacaggtg aatacgttat aatgacagtt 721 tacttccact tgcaaaggaa gatgggctac ttcatgatac agatatacac tccttgcatt 781 atgacagtat ggccgtgagt tgttctgttg tgttacaagt gctggtaaat aaatcatgag 841 caataatctt taaatacata agggtgtggc tgtagctagt aatgctgatg aaaatcagaa 901 agggaatcca agagggtcac aatagtgtta gactttttaa acagtaagag ctgtgtagga 961 atccttctgt gaaccataaa aataataaac gctgagccta ttggaacata atctcaatca 1021 gcaagatgcc aatgctttcc agatttcctc acagggccct gctaattcca tgccaaaact 1081 ggcagtggat ttcttccttc taatctccct taatgcctta ttcgattatt gtcactgctc 1141 tccgaccatg cactgtccct tcagactctc ctttcccaac tgagtcagtc agaggagcct 1201 gggtgtctgc aggacataat ctaagaccac aaaccacctt gttccacgtg agaaggaaac 1261 aagaaagaag ggagccaggg gaatcctgca aatttttagg caacctcttt atctattgga 1321 ttactgactt gaggcaaaca aggaacagag atatgaaggg tttgaaagat ttctccagtt 1381 gattggcaga gaagagctgg ctagcaggga ggacgaccct aggagggtga attctgcatt 1441 tcagtgcact gcaggaacgc gtcacgtggc gctaccggac tcagatctga agttcctatt 1501 ctctagaaag tataggaact tcatgtccaa tttactgacc gtacaccaaa atttgcctgc 1561 attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga 1621 tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc 1681 ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga 1741 ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg 1801 ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt 1861 ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca 1921 ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga 1981 tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg 2041 tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat 2101 gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact 2161 tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga 2221 tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac 2281 cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta 2341 cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca gtgcccgtgt 2401 cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg 2461 tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg 2521 ggcaatggtg cgcctgctgg aagatggcga tggtaccgcg ggcccgggat ccatcgccac 2581 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 2641 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 2701 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 2761 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 2821 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 2881 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 2941 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 3001 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 3061 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 3121 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 3181 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 3241 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtt 3301 aagcggccgc gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtaga 3361 tccaaaaaag aagagaaagg taagcggccg cgactctaga tcataatcag ccataccaca 3421 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 3481 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 3541 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcgaag ttcctattct 3601 ctagaaagta taggacttcg aattctcgta aaactcttca tggcagtagt tattattctc 3661 tctctctctc tttttctttt ttcttgagac aggatatttc tctgttgccc aggctggagt 3721 gcagtggcac agtcttggct cactgcagcc tggacctcct gggctcaagc catcctccca 3781 cctcagcctc ccaagtagct ggggctacag gcacatggcc accaggccca gataattttt 3841 catttttgta gagactgagt ctcaccatgt tacccaggtt tattattctc attttttaga 3901 tgaagagact gaggtccaga gaagctcaat gacttgccta gttttacaaa tctcctgcca 3961 tcacataccc ctcagcgtcc ttaataagag ggaggccacc aactatgtgc tgggcactgt 4021 ggtggatgct ggagctatag ggttgagtat ataagaaatg gtgttgctgg agcaactgtt 4081 gcttgcttac ctgacctatc tgagaattaa ttagcagggg aacatatttt tgttttcaga 4141 ttcaatataa gaacttgtgt gggcaaaaat aaagatcagt agtaataaca gtagttccca 4201 tttgctgact gtactgtcct aagtgcatat atatatacat acacacacgc atacctatac 4261 tcctctaata ctcaaaatga tcctgtttat gtattgttaa tatgctcatt ttatttttaa 4321 atttttattt atttttattt ttatttattt ttgagacgga gtctcattct gtcgcggagg 4381 ctgaagtgca gtggtgcgat ctcagctcag tgcgacctcc gcctcccggg ttcaagtgat 4441 tctcctgcct cagcctccgg actagctggg attacaggcg cccgcctcca cgcccagcta 4501 atttttgtat ttttagtaga gatggggttt cgccatgttg gccaggctgg tctcgtactc 4561 ctgaccttga gtgatccacc tgcctcggcc tcccaaagtg ctgggattac aggcatgagc 4621 caccgcgccg ggctaatatg ctcattttag tgaggcaaaa atagaggctc agagtctgat 4681 ttgtacaaaa ctacagagca gttaagtgtc ctctcagatg tgtaccctga tctgggtgac 4741 tctaggactc taggtctcaa ctgttacaac cagttaaggg tttggggaag cactgggcca 4801 agagtcagga aaatggaagc cacaggtagt gcaaggtctt gggaatggga cgtctggtcc 4861 aaggattcac gcgatgactg gaacccgaag agccggggcc cggtttacgg ccgccatgaa 4921 gcaacgcgcg ccggtaggtt tgggaatcag ggagccctct gaataggaga ctgagttggg 4981 agggaaaggg gcttcgctgg gggagcctcg gcttcttctg ggagaaaatt cccacggcta 5041 cctagtgagc ctgcaaactg gtaggcgccg gcgtaggcgc gcgggcgggg ccgggggcgg 5101 ggcctgcggg gcgtggcggg gcgggcagag ggcggggcct gcttctcctc agcttcaggc 5161 ggctgcgacg agccctcagg cgaacctctc ggctttcccg cgcggcgccg cctcttgctg 5221 cgcctccgcc tcctcctctg ctccgccacc ggcttcctcc tcctgagcag tcagcccgcg 5281 cgccggccgg ctccgttatg gcgacccgca gccctggcgt cgtggtgagc agctcggcct 5341 gccggccctg gccggttcag gcccacgcgg caggtggcgg ccgggccctg aggcgcggga 5401 tcgatccatg acaccccccc gccccccata tctcactata attcactgtc aaatggcttt 5461 tatgatttgg agtttgggga tacttttaaa gacagggtct tggtagctcc tagatggctt 5521 gggattcact tagtaaccct tgctggccta aaattcagga acttctacct catcttccca 5581 agtactgggt tagtagggat gtccagcata tttagcttgc tttaatgatt ttgctgtact 5641 tttttctgtg tgtacaggtg catgtatatt tataaaacta acataatata ttttaccccc 5701 aaagtaccat aaccctaatt aatgctatta aatgttttgt atgctgggct ctgggcatac 5761 taatataacc aatattatac aatgcaaaac aattttggaa tatcttctgt aaaattatct 5821 tattagtaag ttgtaaccat ctcagacttc taagtttgag tttgtaaagg atcagttttt 5881 tttggtgacc tacacacaca cacacacaca cacacacaca cacacacaca ggggggtgga 5941 ttctgagaat ttagagaatt tagatggtta ataccagttt ttatttttat tctgtgtgat 6001 atttatttat taagatttat ttatttatta catgtaggta cactgtatct gtcttcagac 6061 acaccagaag agggcatcag atctcgttac agatggttgt gagtcaccat gtggttgctg 6121 ggatttgaac tcaggacctc tggaagaaca gtcagtgctc ttaaccgttg agccatctca 6181 ccagccccgt gatattttat ttaacttact agaatatact gtgttaaaac atattttagg 6241 ggattgggga gatggcccat tctgtaaagt gtctgccatg tcatcttcag gacctgcgtt 6301 tgtaacccca gcatggagaa gacagagaca gggagacccc tgggcttcct ggcaagctag 6361 actagcaaat ttggttaact aggttcaatg agagactcag tctcaaaaca taagaagtga 6421 attttttaga cccaatttca atctctgaac tccatacgta tgcacatgta cctgcatata 6481 aatatatata cacacccata aacacatgca cacacaaaaa atggttcaaa agttcttaaa 6541 atgtataaga gtagaaagtg tagagccggg agtggtggcg cacgccttta atcccagcac 6601 ttgggagcag aggcaggtgg atttctgagt tcgaggccag cctggtctac agagtgagtt 6661 ccaggacagc cagagctata cagagaaatc ctgcctcaaa aaaccaaaaa taaagtgtat 6721 atactaatct ttcatgccta aaatatttat ttttgccaca tttttcatac ctctttaata 6781 tttttctttt tctctttgtg cttcaaagga aattttgtgt catgttttac ttttcattct 6841 tgactctatt cagcagtaag acgcagcatg gctttcttag agaagtataa tctattacaa 6901 aacggtaaca tttaactgct ctacataccc tcttgtagct aaattcctct gttaaactaa 6961 gacttattat ctaaacgtaa ttaaatgttg atatcttata atgctccact ttgaaacagc 7021 tgtctttctt ttgcagatta gcgatgatga accaggttat gacctagatt tgttttgtat 7081 acctaatcat tatgccgagg atttggaaaa agtgtttatt cctcatggac tgattatgga 7141 caggttagta agacctcgat tgaagttatt tttagagggt gtgtgtgtgg tgtgtgtgtg 7201 tgtgtgtgtg tgtgtgtgtg tgtgtgtgta agagagagag gagaggcatg tgtgatatat 7261 atgtgaagtg tattgcatgt acatggaatc cagagaagga cattaggtgt cctactctgt 7321 tgctctctta ttcccttgaa tcagtgtctc ttgctgaacc tggggcatac atactgattt 7381 ggctaggctg cctggctagc aagacccagg gatctgcttg tctctgcccc ccagtgctga 7441 cgttacaagc aggtgcaacc acatctggtt tttatatggg tactggggga tctgaactta 7501 gggcctgatg cgtgcccaag tgctctcacc cgccaagcca cctctccagc caaagggttt 7561 tttttcttgt attaattcta acttagcaac tgttgagtag ttgatgtttg aatataagct 7621 aagccaactt attagaataa atgttttcta ttttttactt gtatgtgtga gtgcctgcat 7681 gtatgtatgt gaagcatatg tattccagga tgtcagaatt gtgatggatt ccctggaact 7741 ggagttacag accattgtga accactgtgt atgggtgctg ggaactgaac tttaggtcct 7801 ctgcaaaaac agtaaggatc ttaactgata aactatcttt ctagccctaa ggctttctac 7861 ttttaaagtt cgtttctctt tctctctctc tctctctctc tctctctctc tctccctctc 7921 cctccctcct cctcctcctc ctctttcccc cctctcgcct cctctttctc tctcccctcc 7981 ctctctctct ctcacacaca cacacagaca gagagaggag agagaaagga agccttacta 8041 attaattacc tagttttaga cataactagt gggaatccat agtaagtaaa tcaacattct 8101 tgaagagagg ttagaagaat tggctctcgt tgtccatcga ggctgtataa gattattctt 8161 ctgttttctc agcttaccat acatattttc tacgtagaca ataccaattc aaatacactg 8221 tacgtaaagt atatgcgcac acacacacac acacacacac acacacacac acaagttaca 8281 tattgtcata tagtactaaa atttatggac tttttcatat taatacatct agagctatca 8341 tgttttttat cttttaaaaa ttactttacg gggctacaga gatgtctcag cagttaggag 8401 cactgactgc tcttccagag gtcctgagtt caattcccag caaccacatg gtggctcaca 8461 accatctgta atgggatctg atccactctt atggtgtgtc tgaagacagt gacagtgtac 8521 tcatatacat gaaataaatc tttaaaaaat tactttacca tcagggtata tatatatata 8581 tatatgtgtg tgtgtgtgtg tgtgtgtatg aagtatttgc tatttgagag ggtctcactg 8641 tttagctcag tctggcttgg aacgttctgt atagcccaga ctggcctcaa ctcagcagtc 8701 cccctgaaag gctctcatat gtggagatta caggtatgtg ctactgtgcc cagccctaca 8761 tgttactttg tttttgaaat agggtatcaa atagcttaga ctggcttcta agtcagtgtg 8821 tagctaagga tggtctgagc ttctgaacct gctacttcaa ctcctggtgt gccatcatgg 8881 ccagtttgca cagttgtgag agtagaactc aggacctcct atgtgttact agacatgttc 8941 tctaccagct gagctacatc ccaattcgcc tacatttact tttatttttt aaaagattta 9001 tttatgtata cagcactctg cctgcatgta catctgcatg ccagaagagg gcaccagatc 9061 tcatcataca tagttgtgag ccaccatgtg gttgctgggg attgaactca gggcctctgg 9121 aagagcagtc agtgctctta acctctgagc catctctgta gccccctaca tttattttaa 9181 agattatagg ctattctatt catagatgtg ccataactga tttaaccaga tcttcattat 9241 tttgtactta gtagagtata ttagagggaa tgaatcttaa atacacattt tgacacttgc 9301 tcagctttag acaagtaaaa atcactggtc aaggaaatgg tgcttagtgg atgcttctga 9361 actgtcaaat tgtcctccaa caagttacat cattttatac ttccaacgtt atgtgagagt 9421 tcatttttct ttaggctctt ctagtatttt atatttgaga aataaaattt aaccaaaatt 9481 tgccaatcta gtaagcttgt ttttatttgt atctttaaat tttatgatag atgttatagt 9541 gtactctcct ctcccctccc ctcccctctc ctcctcagca ggatcttgtt atgtagccct 9601 aagggatcta atacttactc tgtagtccag gctgtccttc agctcatgag acacaccatc 9661 atgcttggca tgtttcttgt tctgaaaggt atctggtttt aattttggtt gagctggtgt 9721 gttggtgtat atctgtaatc tcagcacagg caagtagaga caaggattat gattacagtc 9781 cagcctgggc tatttagagg gattctcttt aatctttcct catgccccaa aatcttacct 9841 ttggtatatg aaaaatagtc tccacttctg caaaatattg ctttatgaag taagaattaa 9901 ttcccttcat agagacaagg aatgtgtcct gtaaaagttt aatgtgtaag aagtatttgt 9961 tataaaagat aaatattcag aatcttcttt ttaattcctg attttatttc tataggactg 10021 aaagacttgc tcgagatgtc atgaaggaga tgggaggcca tcacattgtg gccctctgtg 10081 tgctcaaggg gggctataag ttctttgctg acctgctgga ttacattaaa gcactgaata 10141 gaaatagtga tagatccatt cctatgactg tagattttat cagactgaag agctactgtg 10201 taagtataat taacttataa ttaaaaaaat agggccattc tagttttatc tatatttttt 10261 ttaaacttgt gcaaactatg ctacataatc aacttacttg attatgtaat atatatagta 10321 atagttggaa ttactattat tacatattta tttttctgtg ctggggtaga acccagggaa 10381 ctaagtactg taataaccga gcttcattca tcctagtcat ttttaatttt tttttttttt 10441 tttgtttttt cgagacaagg tttctctgtg tagccctggc tgtcctggaa ctcactctgt 10501 agaccaggct ggcctcgaac tcagaaatct gcctgcctct gcctcccagt gctgagatta 10561 atgacgtgca ccaccactgc ctggcatttt tttaaagttt taatttgaga ccctttctca 10621 ctaacttgct cacactatcc tcgaactcat tacgtgggct ggtctagaaa tttcagtcaa 10681 cttgcctcag cttctcaagt agctgggatt acaggcaggt gctaccactt ctggccatga 10741 tcattgttgt tgttgctatt attattatta ttatttcact gatacttcat atcacaacac 10801 agaatagtaa ctgtagttca tgaccaacaa aaaagttgta cagtttctct gttgtcttat 10861 tttgatctgg attttgattt gtttcatggc actgggcagg ctaatctcaa actgcatggc 10921 tcaaatagtc cttctgtctt aatctcccat gttctggaag tataggtatc actacaccta 10981 gctgtatttt catattttaa aaaaaacgcc taagaattgc tattgaatga ggcggagaga 11041 tggctcagca gtcacaagta cttgtagagg actggggttt ggttactagc acctcaaaga 11101 tggcttacaa ttatctgtag ctccaattcc agaggatctg tgtggtaaag ctgcatgtat 11161 atgggcatgc aggtaaaact gctaaacata taaaaatagc tcagtcttaa gagaattggt 11221 cgactttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 11281 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 11341 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 11401 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta 11461 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 11521 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 11581 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 11641 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 11701 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 11761 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 11821 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 11881 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 11941 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 12001 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 12061 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 12121 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 12181 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 12241 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 12301 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 12361 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 12421 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 12481 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 12541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 12601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accaggtcga gtagggataa 12661 cagggtaatg gatcctcgtt gggcacctgc actcatgtac gcaggcccac atatagatgt 12721 acacacgcat atcattcaaa atagaaatta tttatacaag tagtgcgttg gagagctggc 12781 tcaggagttg ttaatgcttg ctacccttgc aaaggaccca agttcaattt ccagcacctg 12841 tgttgggcat ctcacaacca catgtaaatc tagtttcagg ggatttcacc cctcttctga 12901 actcttcagg cacttatacc cacaaatgga catctccaca tagagacaca tacttttaaa 12961 aaagtaatct agggtctaga gaaatggtta tagaccacgg actgggatca gttcccagaa 13021 gccacatcct gtggctcaca attgcctgtg aatcaagttc tagatctggt ggcctcgtct 13081 agcctgcagt catcaacaaa cacatgcaca catccataca cacataattt aaaagaaagc 13141 gaaagtaacc tataatgcta aacaaagcac attctcagtg tagactgcag tggtgatgaa 13201 ccttgaaaat atattaatag aagccagaca cgtaagtcac aaatggtata actcaattta 13261 tatgaaattc ccagagagtc caaagttaaa aaaaacaaaa aggcagatta ttggttgtca 13321 gtagctacag agatgggaca ttgaagatat taataggtat agggttgagg attcatggcc 13381 tttccctctt ccatgttagc atgtctattg ttgttaacct tattcaactc atgttagggc 13441 aatcatattg atgagactta atgagtatag cttgacattc ctagaagaca gagtctcaca 13501 tcagactctc tgatcctctg actcttagaa tctttcaaca ccctcttctg aaatgatctc 13561 tgagcctttg gttgtgactg aattatgaga aagaatttta tgttgaatga attttacttc 13621 agcattttta caaaactgaa gctggggatg gcgtttcatg cgtgcagtcc ctaccatttg 13681 cctataagcc tggaggaggc agaagtagaa ttaggctttt cagggacatc ttcagctgca 13741 tcatgagctt gaggacaact tgagctacat aagattccca tccccacccc cacacacaca 13801 cacacatcta gtatgtacct agtatgcagt cttttcatat gtttaaaatg tagaaaggta 13861 tatgtgaagt ctcccttttc cccacaactt agtccctcca aagtattgca tcagtttcct 13921 tgtacatgat tttatgcata tataagatac actatttctc tctctctctc tctccttccc 13981 tccttccctc caacctctgg gtatgagttg agggttatgt atgtttcaag ttgtaataag 14041 aattgccact caaacatttc atcccatttt aagttaagtt tgtctgattt tccactttag 14101 aagggcagcc atgatggctc atgcctataa tcccatccta gcacttgtaa gcctaataca 14161 taagactgga ggattgtcat cagcttgagg ctagcctggt ctacctaatg agttccagcc 14221 agccaagagt agaatataag accctgtctc caaaaaatgt accaaagggg ctggaggggt 14281 aactcagtgg ttaagaacag tagctgctct tccagaggac ctgggttcaa ttcccagcac 14341 ccacatggca gcttgcaact atctgtaact gtccagcttt gggatatccg acaccattat 14401 accaattaca taaaatgaaa aaattgtaaa atgtgctaaa atgagtgggg agggggagca 14461 tggtgtggtt ctgtgggctt gggattttgc catgaatcca tgactatatc tcagcaatat 14521 gtggattatt tctggaaacc tgtggggcat gggtgtatgg gaagagggga gagagagaga 14581 gcccgcatcc agccagagtt cctgtgctct gggcaggcgg acgtgggagg actgctggac 14641 gatttccact tggccctggg tgggcatctg gctgtgtgaa gccactgacc ccactcagcg 14701 gggggtagat aaggggcatt tcccatacca ggttctcaga ctccagcatt ccacgctgga 14761 tacagcagaa gagctgagaa cagaataggg gccccaggga gacactctcg tcccagagat 14821 aaagaaggca gagggagaga gattcctacg caggcgagag gccttagtct gggccttgat 14881 gaacagagac ggtctatggt tttagagctt tattgtagaa aggcaggggg aaagagagaa 14941 ggtagaaaga gagagaccag ccatggccaa gaggagagaa ggggggaaag gagagagaga 15001 agaaaggcta gagagtaaga aaggttaaag agacagaagt aaaagcttag agtaagagag 15061 agagagagag caagagggag agtaagaaaa gtaaaagctt aagagagcga ggaggggcca 15121 gcccctctaa tagtgggctt gttatcttgt tgttgctagg taactgtgaa gcgtctagcc 15181 tgaaggccag aagcttggga cattgtctat gtgactgaaa gccatgagcc tctactgtgg 15241 gggctgtgga ggtggtaact tggacaggag ccaggggttc caggagacat gagggaatgc 15301 cttctgtccc atgtaggtag gaattaccac caccggcttt caccgtagtt cactggagac 15361 tgtttgtaca tagcccattg ccccacagaa accaggcact cactgtttca tttgcataag 15421 aattagaagg ctttattttg gctgggattg gtgacttgcc ccagaaattc cagcttttgg 15481 gaagccgaat cctggagaac caggagttca aggtcatcct caggtacctc atttgttcaa 15541 gaccagcctg tgctaccatg agaccttgtc cctactcccc tcaaccccaa aacacgctgt 15601 tttcatttta taggttgtac taaccatgga ggatggtcat ttgcctttct cttgatttca 15661 gaagcgtagc gcgggctttg caccatgatc tagtgtttcc agataggctt gcctctccac 15721 agccaggctt atcctgcttc agtcccatgt ttggcaaggg caacatatga ctgaagaact 15781 tgcatagcct tatggtgtat catgactctt agccccagtt tataactgtc taacagctgg 15841 tggcttttgt gaaaaaggaa agcatcattt gaacttttaa cttttcagct gtttgttttg 15901 actttgcata aatactgaga ctagctagga aatgagcttt tgtttcattt gctaaaaatg 15961 attttttttt catgaggcaa tttcaagcaa tttgaattta gcaatctcta cttgtctgaa 16021 aagaagttat taattaaaaa aaaacaaaca aacaggaacc tattatgctg gctagtcaca 16081 tgaaagaatc caaatgaaca cagtcaaggc tgaatcaaca catgaaatta ttttctaatt 16141 ccccaaatta catttatgct aattagtatg tagtaaaaca ttttaattgt ttccaatgtt 16201 aaacattaca gaaataattt tgatgttttc ctaataaccc agcctttgat catgtttgca 16261 tttatttgat tgttgtcagt ttattaaata attaagtaag agcaacaaga taaaatgact 16321 tcatgagagt ccttttctgt aacttagaac gtcagtagtc ataggaactg cggtcgttcc 16381 cttacctttc agaagtaggc taaagagttg aacgcaaagg tggtttttcg aatcttcctg 16441 atccttaagc ccccactagg tttatattct atatttgata tcggattcga acccctcgag 16501 cttaggatcc ggaaccctta atataacttc gtataatgta tgctatacga agttattagg 16561 tccctcgaag aggttcacta gtactggcca ttgcggccgc ttcgaggctg cctcgcgcgt 16621 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 16681 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 16741 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact 16801 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca 16861 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc 16921 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 16981 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 17041 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 17101 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 17161 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 17221 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 17281 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 17341 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 17401 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 17461 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 17521 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 17581 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 17641 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 17701 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 17761 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 17821 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 17881 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 17941 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 18001 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 18061 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 18121 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg 18181 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 18241 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 18301 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 18361 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 18421 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa 18481 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 18541 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 18601 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 18661 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 18721 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 18781 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgg //